Restriction map for
pF1KB7836
(Circular) (Six-base) MAPSORT of: pF1KB7836.seq Check: 6708 from: 1 to: 4949
pF1KB7836 4949 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1987 1987
Size: 4949
Acc65I G'GTAC_C
Cuts at: 1965 1965
Size: 4949
AclI AA'CG_TT
Cuts at: 4789 4789
Size: 4949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 1011 1263 1515 2774 3206 3953 4221 927
Size: 84 252 252 1259 432 747 268 1655
Fragments arranged by size:
1655 1259 747 432 268 252 252 84
AflII C'TTAA_G
Cuts at: 1446 1446
Size: 4949
AflIII A'CryG_T
Cuts at: 760 1768 2301 3405 4382 760
Size: 1008 533 1104 977 1327
Fragments arranged by size:
1327 1104 1008 977 533
AgeI A'CCGG_T
Cuts at: 3337 3337
Size: 4949
AlwNI CAG_nnn'CTG
Cuts at: 2254 3821 2254
Size: 1567 3382
ApaLI G'TGCA_C
Cuts at: 3719 3719
Size: 4949
ApoI r'AATT_y
Cuts at: 525 592 699 705 1145 1229 1313 1481 1649
Size: 67 107 6 440 84 84 168 168
Cuts at: 1649 1817 1901 1953 4302 4558 525
Size: 168 84 52 2349 256 916
Fragments arranged by size:
2349 916 440 256 168 168 168 107
84 84 84 67 52 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4949
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 1969 1969
Size: 4949
BamHI G'GATC_C
Cuts at: 1974 1974
Size: 4949
BanI G'GyrC_C
Cuts at: 1965 2379 2627 2662 1965
Size: 414 248 35 4252
Fragments arranged by size:
4252 414 248 35
BanII G_rGCy'C
Cuts at: 1963 2993 1963
Size: 1030 3919
BbeI G_GCGC'C
Cuts at: 2383 2631 2383
Size: 248 4701
BbsI GAAGACnn'nnnn_
Cuts at: 615 615
Size: 4949
BciVI GTATCCnnnnn_n'
Cuts at: 2847 3608 4949 2847
Size: 761 1341 2847
Fragments arranged by size:
2847 1341 761
BclI T'GATC_A
Cuts at: 2469 2469
Size: 4949
BfrBI ATG'CAT
Cuts at: 1569 1852 1569
Size: 283 4666
BglII A'GATC_T
Cuts at: 2464 4501 2464
Size: 2037 2912
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 2574 2667 3723 2574
Size: 93 1056 3800
Fragments arranged by size:
3800 1056 93
BmgBI CAC'GTC
Cuts at: 162 162
Size: 4949
BmrI ACTGGGnnnn_n'
Cuts at: 274 2161 2242 2577 4721 274
Size: 1887 81 335 2144 502
Fragments arranged by size:
2144 1887 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1310 1562 3316 1310
Size: 252 1754 2943
Fragments arranged by size:
2943 1754 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 839 1259 1427 1511 1595 1679 1847 259
Size: 580 420 168 84 84 84 168 3361
Fragments arranged by size:
3361 580 420 168 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2125 3004 3496 3794 1055
Size: 1070 879 492 298 2210
Fragments arranged by size:
2210 1070 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 4653 264
Size: 4389 560
BsaAI yAC'GTr
Cuts at: 2302 2302
Size: 4949
BsaBI GATnn'nnATC
Cuts at: 6 2463 4500 6
Size: 2457 2037 455
Fragments arranged by size:
2457 2037 455
BsaHI Gr'CG_yC
Cuts at: 350 2380 2628 3330 4826 350
Size: 2030 248 702 1496 473
Fragments arranged by size:
2030 1496 702 473 248
BsaWI w'CCGG_w
Cuts at: 1321 2138 2659 3337 3611 3758 4169 1321
Size: 817 521 678 274 147 411 2101
Fragments arranged by size:
2101 817 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 345 345
Size: 4949
BseYI C'CCAG_C
Cuts at: 115 2374 3132 3709 4205 115
Size: 2259 758 577 496 859
Fragments arranged by size:
2259 859 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 1730
Size: 4949
BsiEI CG_ry'CG
Cuts at: 104 2491 2537 3745 104
Size: 2387 46 1208 1308
Fragments arranged by size:
2387 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 376 1963 2741 2931 3723 173
Size: 203 1587 778 190 792 1399
Fragments arranged by size:
1587 1399 792 778 203 190
BsmI GAATG_Cn'
Cuts at: 1571 1571
Size: 4949
BsmBI CGTCTCn'nnnn_
Cuts at: 357 357
Size: 4949
Bsp1286I G_dGCh'C
Cuts at: 173 376 1963 2574 2667 2741 2931 2993 3723
Size: 203 1587 611 93 74 190 62 730
Cuts at: 3723 173
Size: 1399
Fragments arranged by size:
1587 1399 730 611 203 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4169 4169
Size: 4949
BspHI T'CATG_A
Cuts at: 1473 4125 1473
Size: 2652 2297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1999 2213 2515 2896 1999
Size: 214 302 381 4052
Fragments arranged by size:
4052 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1546 3241 3295 4779 4944 50
Size: 1496 1695 54 1484 165 55
Fragments arranged by size:
1695 1496 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1350 2861 1350
Size: 1511 3438
BsrFI r'CCGG_y
Cuts at: 2947 3128 3337 2947
Size: 181 209 4559
Fragments arranged by size:
4559 209 181
BssSI C'ACGA_G
Cuts at: 3220 3578 3220
Size: 358 4591
BstAPI GCAn_nnn'nTGC
Cuts at: 2326 2326
Size: 4949
BstBI TT'CG_AA
Cuts at: 3310 3310
Size: 4949
BstYI r'GATC_y
Cuts at: 1 318 475 1974 2441 2464 2799 3045 4057
Size: 317 157 1499 467 23 335 246 1012
Cuts at: 4057 4143 4155 4501 1
Size: 86 12 346 449
Fragments arranged by size:
1499 1012 467 449 346 335 317 246
157 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1613 2899 3080 3243 1613
Size: 1286 181 163 3319
Fragments arranged by size:
3319 1286 181 163
BtsI GCAGTG_nn'
Cuts at: 2192 2192
Size: 4949
ClaI AT'CG_AT
Cuts at: 1674 1674
Size: 4949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 1332
Size: 35 4914
DraI TTT'AAA
Cuts at: 834 851 1254 1758 1948 834
Size: 17 403 504 190 3835
Fragments arranged by size:
3835 504 403 190 17
DrdI GACnn_nn'nnGTC
Cuts at: 2655 3513 2655
Size: 858 4091
EaeI y'GGCC_r
Cuts at: 597 1701 2534 2708 3099 3126 4471 597
Size: 1104 833 174 391 27 1345 1075
Fragments arranged by size:
1345 1104 1075 833 391 174 27
EagI C'GGCC_G
Cuts at: 2534 2534
Size: 4949
EarI CTCTTCn'nnn_
Cuts at: 485 1907 2972 3182 485
Size: 1422 1065 210 2252
Fragments arranged by size:
2252 1422 1065 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3157 3467 3613 4759 3157
Size: 310 146 1146 3347
Fragments arranged by size:
3347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1961 1961
Size: 4949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 839 927 1011 1259 1263 1427 1511 1515
Size: 580 88 84 248 4 164 84 4
Cuts at: 1515 1595 1679 1847 2774 3206 3953 4221 259
Size: 80 84 168 927 432 747 268 987
Fragments arranged by size:
987 927 747 580 432 268 248 168
164 88 84 84 84 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 134 977 2088 134
Size: 843 1111 2995
Fragments arranged by size:
2995 1111 843
EcoRI G'AATT_C
Cuts at: 699 1229 1313 1481 1649 1817 1953 699
Size: 530 84 168 168 168 136 3695
Fragments arranged by size:
3695 530 168 168 168 136 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 2968 1185
Size: 1783 3166
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 2383 2631 3653 4632 2383
Size: 248 1022 979 2700
Fragments arranged by size:
2700 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2791 2821 2823 2853 2791
Size: 30 2 30 4887
Fragments arranged by size:
4887 30 30 2
HincII GTy'rAC
Cuts at: 395 1988 395
Size: 1593 3356
HindIII A'AGCT_T
Cuts at: 854 854
Size: 4949
KasI G'GCGC_C
Cuts at: 2379 2627 2379
Size: 248 4701
KpnI G_GTAC'C
Cuts at: 1969 1969
Size: 4949
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3620 3804 3620
Size: 184 4765
MscI TGG'CCA
Cuts at: 599 1703 2710 599
Size: 1104 1007 2838
Fragments arranged by size:
2838 1104 1007
MslI CAynn'nnrTG
Cuts at: 584 666 1506 1617 1674 2938 3065 584
Size: 82 840 111 57 1264 127 2468
Fragments arranged by size:
2468 1264 840 127 111 82 57
MspA1I CmG'CkG
Cuts at: 123 2060 2374 2734 3747 3992 123
Size: 1937 314 360 1013 245 1080
Fragments arranged by size:
1937 1080 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3130 3130
Size: 4949
NarI GG'CG_CC
Cuts at: 2380 2628 2380
Size: 248 4701
NcoI C'CATG_G
Cuts at: 107 107
Size: 4949
NgoMIV G'CCGG_C
Cuts at: 3128 3128
Size: 4949
NsiI A_TGCA'T
Cuts at: 1571 1854 1571
Size: 283 4666
NspI r_CATG'y
Cuts at: 764 1772 1856 1942 2002 2502 3409 764
Size: 1008 84 86 60 500 907 2304
Fragments arranged by size:
2304 1008 907 500 86 84 60
PasI CC'CwG_GG
Cuts at: 323 323
Size: 4949
PciI A'CATG_T
Cuts at: 760 1768 3405 760
Size: 1008 1637 2304
Fragments arranged by size:
2304 1637 1008
PflMI CCAn_nnn'nTGG
Cuts at: 987 987
Size: 4949
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 4088 4120 244
Size: 32 3812 32 1073
Fragments arranged by size:
3812 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 977 977
Size: 4949
PsiI TTA'TAA
Cuts at: 1898 1898
Size: 4949
PstI C_TGCA'G
Cuts at: 1996 2681 1996
Size: 685 4264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 2374 2734 2374
Size: 360 4589
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 1963 1963
Size: 4949
SalI G'TCGA_C
Cuts at: 1986 1986
Size: 4949
SapI GCTCTTCn'nnn_
Cuts at: 2972 3182 2972
Size: 210 4739
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 4466 4466
Size: 4949
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 4949
SfcI C'TryA_G
Cuts at: 33 246 566 999 1335 1992 2677 3670 3861
Size: 213 320 433 336 657 685 993 191
Cuts at: 3861 33
Size: 1121
Fragments arranged by size:
1121 993 685 657 433 336 320 213
191
SfoI GGC'GCC
Cuts at: 2381 2629 2381
Size: 248 4701
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1971 1971
Size: 4949
SmlI C'TyrA_G
Cuts at: 1070 1446 2104 3019 3511 3773 1070
Size: 376 658 915 492 262 2246
Fragments arranged by size:
2246 915 658 492 376 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 2002 2502 2002
Size: 500 4449
StyI C'CwwG_G
Cuts at: 107 2083 2437 107
Size: 1976 354 2619
Fragments arranged by size:
2619 1976 354
SwaI ATTT'AAAT
Cuts at: 834 834
Size: 4949
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2532 2665 3124 3333 2532
Size: 133 459 209 4148
Fragments arranged by size:
4148 459 209 133
TatI w'GTAC_w
Cuts at: 757 4464 757
Size: 3707 1242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 4466 4498 772
Size: 32 3662 32 1223
Fragments arranged by size:
3662 1223 32 32
Tth111I GACn'n_nGTC
Cuts at: 390 2746 390
Size: 2356 2593
XbaI T'CTAG_A
Cuts at: 1980 1980
Size: 4949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2444 2444
Size: 4949
XmaI C'CCGG_G
Cuts at: 1969 1969
Size: 4949
XmnI GAAnn'nnTTC
Cuts at: 940 1313 940
Size: 373 4576
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI ZraI