Restriction map for
pF1KB7853
(Circular) (Six-base) MAPSORT of: pF1KB7853.seq Check: 7277 from: 1 to: 4760
pF1KB7853 4760 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 970 1342 970
Size: 372 4388
AccI GT'mk_AC
Cuts at: 1798 1798
Size: 4760
Acc65I G'GTAC_C
Cuts at: 1776 1776
Size: 4760
AclI AA'CG_TT
Cuts at: 4600 4600
Size: 4760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 2585 3017 3764 4032 1037
Size: 1548 432 747 268 1765
Fragments arranged by size:
1765 1548 747 432 268
AflIII A'CryG_T
Cuts at: 2112 3216 4193 2112
Size: 1104 977 2679
Fragments arranged by size:
2679 1104 977
AgeI A'CCGG_T
Cuts at: 3148 3148
Size: 4760
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 157
Size: 32 4728
AlwNI CAG_nnn'CTG
Cuts at: 190 375 483 628 2065 3632 190
Size: 185 108 145 1437 1567 1318
Fragments arranged by size:
1567 1437 1318 185 145 108
ApaLI G'TGCA_C
Cuts at: 1419 3530 1419
Size: 2111 2649
ApoI r'AATT_y
Cuts at: 612 901 1002 1365 1764 4113 4369 612
Size: 289 101 363 399 2349 256 1003
Fragments arranged by size:
2349 1003 399 363 289 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 4760
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
AvaI C'yCGr_G
Cuts at: 1704 1780 1704
Size: 76 4684
AvrII C'CTAG_G
Cuts at: 132 132
Size: 4760
BamHI G'GATC_C
Cuts at: 1785 1785
Size: 4760
BanI G'GyrC_C
Cuts at: 588 1776 2190 2438 2473 588
Size: 1188 414 248 35 2875
Fragments arranged by size:
2875 1188 414 248 35
BanII G_rGCy'C
Cuts at: 575 1031 1311 1705 1774 2804 575
Size: 456 280 394 69 1030 2531
Fragments arranged by size:
2531 1030 456 394 280 69
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4512
BbsI GAAGACnn'nnnn_
Cuts at: 1357 1357
Size: 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4726
BciVI GTATCCnnnnn_n'
Cuts at: 511 2658 3419 4760 511
Size: 2147 761 1341 511
Fragments arranged by size:
2147 1341 761 511
BclI T'GATC_A
Cuts at: 725 2280 725
Size: 1555 3205
BglI GCCn_nnn'nGGC
Cuts at: 1315 1315
Size: 4760
BglII A'GATC_T
Cuts at: 2275 4312 2275
Size: 2037 2723
BlpI GC'TnA_GC
Cuts at: 1872 1872
Size: 4760
Bme1580I G_kGCm'C
Cuts at: 1423 2385 2478 3534 1423
Size: 962 93 1056 2649
Fragments arranged by size:
2649 1056 962 93
BmrI ACTGGGnnnn_n'
Cuts at: 165 825 1972 2053 2388 4532 165
Size: 660 1147 81 335 2144 393
Fragments arranged by size:
2144 1147 660 393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 225 1150 3127 225
Size: 925 1977 1858
Fragments arranged by size:
1977 1858 925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 733 850 1673 1719 195
Size: 538 117 823 46 3236
Fragments arranged by size:
3236 823 538 117 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1101 1175 1288 1936 2815 3307 3605 628
Size: 473 74 113 648 879 492 298 1783
Fragments arranged by size:
1783 879 648 492 473 298 113 74
BsaI GGTCTCn'nnnn_
Cuts at: 214 271 725 826 855 1276 4464 214
Size: 57 454 101 29 421 3188 510
Fragments arranged by size:
3188 510 454 421 101 57 29
BsaAI yAC'GTr
Cuts at: 2113 2113
Size: 4760
BsaBI GATnn'nnATC
Cuts at: 6 499 2274 4311 6
Size: 493 1775 2037 455
Fragments arranged by size:
2037 1775 493 455
BsaHI Gr'CG_yC
Cuts at: 2191 2439 3141 4637 2191
Size: 248 702 1496 2314
Fragments arranged by size:
2314 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1399 1949 2470 3148 3422 3569 3980 1399
Size: 550 521 678 274 147 411 2179
Fragments arranged by size:
2179 678 550 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1178 1285 1178
Size: 107 4653
BseYI C'CCAG_C
Cuts at: 698 759 2185 2943 3520 4016 698
Size: 61 1426 758 577 496 1442
Fragments arranged by size:
1442 1426 758 577 496 61
BsiEI CG_ry'CG
Cuts at: 104 2302 2348 3556 104
Size: 2198 46 1208 1308
Fragments arranged by size:
2198 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1031 1423 1705 1774 2552 2742 3534 1031
Size: 392 282 69 778 190 792 2257
Fragments arranged by size:
2257 792 778 392 282 190 69
Bsp1286I G_dGCh'C
Cuts at: 575 1031 1311 1423 1705 1774 2385 2478 2552
Size: 456 280 112 282 69 611 93 74
Cuts at: 2552 2742 2804 3534 575
Size: 190 62 730 1801
Fragments arranged by size:
1801 730 611 456 282 280 190 112
93 74 69 62
BspEI T'CCGG_A
Cuts at: 3980 3980
Size: 4760
BspHI T'CATG_A
Cuts at: 273 3936 273
Size: 3663 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 970 1342 1810 2024 2326 2707 970
Size: 372 468 214 302 381 3023
Fragments arranged by size:
3023 468 381 372 302 214
BsrBI CCG'CTC
Cuts at: 50 3052 3106 4590 4755 50
Size: 3002 54 1484 165 55
Fragments arranged by size:
3002 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 496 2672 496
Size: 2176 2584
BsrFI r'CCGG_y
Cuts at: 2758 2939 3148 2758
Size: 181 209 4370
Fragments arranged by size:
4370 209 181
BssSI C'ACGA_G
Cuts at: 1552 3031 3389 1552
Size: 1479 358 2923
Fragments arranged by size:
2923 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1427 2137 1427
Size: 710 4050
BstBI TT'CG_AA
Cuts at: 3121 3121
Size: 4760
BstYI r'GATC_y
Cuts at: 1 344 353 395 819 947 1726 1785 2252
Size: 343 9 42 424 128 779 59 467
Cuts at: 2252 2275 2610 2856 3868 3954 3966 4312 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 779 467 449 424 346 343 335
246 128 86 59 42 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 593 621 770 1139 593
Size: 28 149 369 4214
Fragments arranged by size:
4214 369 149 28
BtgI C'CryG_G
Cuts at: 1313 1313
Size: 4760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 131 2710 2891 3054 131
Size: 2579 181 163 1837
Fragments arranged by size:
2579 1837 181 163
BtsI GCAGTG_nn'
Cuts at: 449 751 2003 449
Size: 302 1252 3206
Fragments arranged by size:
3206 1252 302
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4760
DraIII CAC_nnn'GTG
Cuts at: 651 1262 651
Size: 611 4149
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3324 2466
Size: 858 3902
EaeI y'GGCC_r
Cuts at: 2345 2519 2910 2937 4282 2345
Size: 174 391 27 1345 2823
Fragments arranged by size:
2823 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4760
EarI CTCTTCn'nnn_
Cuts at: 431 905 1270 2783 2993 431
Size: 474 365 1513 210 2198
Fragments arranged by size:
2198 1513 474 365 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2968 3278 3424 4570 2968
Size: 310 146 1146 3158
Fragments arranged by size:
3158 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1029 1703 1772 1029
Size: 674 69 4017
Fragments arranged by size:
4017 674 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 733 850 1037 1673 1719 2585 3017 3764
Size: 538 117 187 636 46 866 432 747
Cuts at: 3764 4032 195
Size: 268 923
Fragments arranged by size:
923 866 747 636 538 432 268 187
117 46
EcoNI CCTnn'n_nnAGG
Cuts at: 199 1241 1359 199
Size: 1042 118 3600
Fragments arranged by size:
3600 1042 118
EcoO109I rG'GnC_Cy
Cuts at: 129 471 625 738 1899 129
Size: 342 154 113 1161 2990
Fragments arranged by size:
2990 1161 342 154 113
EcoRI G'AATT_C
Cuts at: 612 1365 1764 612
Size: 753 399 3608
Fragments arranged by size:
3608 753 399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1764 2779 1764
Size: 1015 3745
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4760
HaeII r_GCGC'y
Cuts at: 2194 2442 3464 4443 2194
Size: 248 1022 979 2511
Fragments arranged by size:
2511 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 506 511 528 538 543 560 601 633 2602
Size: 5 17 10 5 17 41 32 1969
Cuts at: 2602 2632 2634 2664 506
Size: 30 2 30 2602
Fragments arranged by size:
2602 1969 41 32 30 30 17 17
10 5 5 2
HincII GTy'rAC
Cuts at: 117 1799 117
Size: 1682 3078
HindIII A'AGCT_T
Cuts at: 459 1049 459
Size: 590 4170
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4512
KpnI G_GTAC'C
Cuts at: 1780 1780
Size: 4760
MluI A'CGCG_T
Cuts at: 4193 4193
Size: 4760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 3431 3615 344
Size: 3087 184 1489
Fragments arranged by size:
3087 1489 184
MscI TGG'CCA
Cuts at: 2521 2521
Size: 4760
MslI CAynn'nnrTG
Cuts at: 938 2749 2876 938
Size: 1811 127 2822
Fragments arranged by size:
2822 1811 127
MspA1I CmG'CkG
Cuts at: 1182 1697 1871 2185 2545 3558 3803 1182
Size: 515 174 314 360 1013 245 2139
Fragments arranged by size:
2139 1013 515 360 314 245 174
NaeI GCC'GGC
Cuts at: 2941 2941
Size: 4760
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4512
NcoI C'CATG_G
Cuts at: 1313 1313
Size: 4760
NgoMIV G'CCGG_C
Cuts at: 2939 2939
Size: 4760
NspI r_CATG'y
Cuts at: 1039 1813 2313 3220 1039
Size: 774 500 907 2579
Fragments arranged by size:
2579 907 774 500
PciI A'CATG_T
Cuts at: 3216 3216
Size: 4760
PflMI CCAn_nnn'nTGG
Cuts at: 430 1262 430
Size: 832 3928
PfoI T'CCnGG_A
Cuts at: 423 423
Size: 4760
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4728
PpuMI rG'GwC_Cy
Cuts at: 471 738 471
Size: 267 4493
PsiI TTA'TAA
Cuts at: 1667 1667
Size: 4760
PspXI vC'TCGA_Gb
Cuts at: 1704 1704
Size: 4760
PstI C_TGCA'G
Cuts at: 1339 1807 2492 1339
Size: 468 685 3607
Fragments arranged by size:
3607 685 468
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4760
PvuII CAG'CTG
Cuts at: 1182 1697 2185 2545 1182
Size: 515 488 360 3397
Fragments arranged by size:
3397 515 488 360
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4760
SacI G_AGCT'C
Cuts at: 1031 1705 1774 1031
Size: 674 69 4017
Fragments arranged by size:
4017 674 69
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4760
SanDI GG'GwC_CC
Cuts at: 471 471
Size: 4760
SapI GCTCTTCn'nnn_
Cuts at: 905 2783 2993 905
Size: 1878 210 2672
Fragments arranged by size:
2672 1878 210
SbfI CC_TGCA'GG
Cuts at: 1339 1807 1339
Size: 468 4292
ScaI AGT'ACT
Cuts at: 4277 4277
Size: 4760
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 4760
SfcI C'TryA_G
Cuts at: 33 316 1335 1803 2488 3481 3672 33
Size: 283 1019 468 685 993 191 1121
Fragments arranged by size:
1121 1019 993 685 468 283 191
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4512
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4760
SmlI C'TyrA_G
Cuts at: 607 1080 1190 1267 1704 1915 2830 3322 3584
Size: 473 110 77 437 211 915 492 262
Cuts at: 3584 607
Size: 1783
Fragments arranged by size:
1783 915 492 473 437 262 211 110
77
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4760
SphI G_CATG'C
Cuts at: 1039 1813 2313 1039
Size: 774 500 3486
Fragments arranged by size:
3486 774 500
StuI AGG'CCT
Cuts at: 137 137
Size: 4760
StyI C'CwwG_G
Cuts at: 132 1313 1894 2248 132
Size: 1181 581 354 2644
Fragments arranged by size:
2644 1181 581 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 396 2343 2476 2935 3144 396
Size: 1947 133 459 209 2012
Fragments arranged by size:
2012 1947 459 209 133
TatI w'GTAC_w
Cuts at: 4275 4275
Size: 4760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4277 4309 4277
Size: 32 4728
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4760
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 262 2255 262
Size: 1993 2767
XhoI C'TCGA_G
Cuts at: 1704 1704
Size: 4760
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4760
XmnI GAAnn'nnTTC
Cuts at: 1606 1690 1606
Size: 84 4676
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspOMI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI