Restriction map for
pF1KB7854
(Circular) (Six-base) MAPSORT of: pF1KB7854.seq Check: 2289 from: 1 to: 4988
pF1KB7854 4988 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2026 2026
Size: 4988
Acc65I G'GTAC_C
Cuts at: 2004 2004
Size: 4988
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 785 1002 2813 3245 3992 4260 608
Size: 177 217 1811 432 747 268 1336
Fragments arranged by size:
1811 1336 747 432 268 217 177
AflIII A'CryG_T
Cuts at: 821 2340 3444 4421 821
Size: 1519 1104 977 1388
Fragments arranged by size:
1519 1388 1104 977
AgeI A'CCGG_T
Cuts at: 3376 3376
Size: 4988
AleI CACnn'nnGTG
Cuts at: 577 577
Size: 4988
AlwNI CAG_nnn'CTG
Cuts at: 2293 3860 2293
Size: 1567 3421
ApaI G_GGCC'C
Cuts at: 904 904
Size: 4988
ApaLI G'TGCA_C
Cuts at: 622 3758 622
Size: 3136 1852
ApoI r'AATT_y
Cuts at: 504 787 1992 4341 4597 504
Size: 283 1205 2349 256 895
Fragments arranged by size:
2349 1205 895 283 256
AseI AT'TA_AT
Cuts at: 20 269 20
Size: 249 4739
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 329 538 632 2008 329
Size: 209 94 1376 3309
Fragments arranged by size:
3309 1376 209 94
BamHI G'GATC_C
Cuts at: 2013 2013
Size: 4988
BanI G'GyrC_C
Cuts at: 403 2004 2418 2666 2701 403
Size: 1601 414 248 35 2690
Fragments arranged by size:
2690 1601 414 248 35
BanII G_rGCy'C
Cuts at: 547 904 2002 3032 547
Size: 357 1098 1030 2503
Fragments arranged by size:
2503 1098 1030 357
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BbsI GAAGACnn'nnnn_
Cuts at: 383 1008 1031 1344 383
Size: 625 23 313 4027
Fragments arranged by size:
4027 625 313 23
BbvCI CC'TCA_GC
Cuts at: 812 812
Size: 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 529 563 529
Size: 34 4954
BciVI GTATCCnnnnn_n'
Cuts at: 2886 3647 4988 2886
Size: 761 1341 2886
Fragments arranged by size:
2886 1341 761
BclI T'GATC_A
Cuts at: 828 2508 828
Size: 1680 3308
BfrBI ATG'CAT
Cuts at: 1762 1762
Size: 4988
BglI GCCn_nnn'nGGC
Cuts at: 253 253
Size: 4988
BglII A'GATC_T
Cuts at: 2503 4540 2503
Size: 2037 2951
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4988
Bme1580I G_kGCm'C
Cuts at: 155 626 692 904 2613 2706 3762 155
Size: 471 66 212 1709 93 1056 1381
Fragments arranged by size:
1709 1381 1056 471 212 93 66
BmgBI CAC'GTC
Cuts at: 687 1188 687
Size: 501 4487
BmrI ACTGGGnnnn_n'
Cuts at: 155 2200 2281 2616 4760 155
Size: 2045 81 335 2144 383
Fragments arranged by size:
2144 2045 383 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1926 3355 1926
Size: 1429 3559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 4988
Bpu10I CC'TnA_GC
Cuts at: 812 812
Size: 4988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1248 2164 3043 3535 3833 1248
Size: 916 879 492 298 2403
Fragments arranged by size:
2403 916 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 946 4692 946
Size: 3746 1242
BsaAI yAC'GTr
Cuts at: 1731 2341 1731
Size: 610 4378
BsaBI GATnn'nnATC
Cuts at: 6 1210 2502 4539 6
Size: 1204 1292 2037 455
Fragments arranged by size:
2037 1292 1204 455
BsaHI Gr'CG_yC
Cuts at: 2419 2667 3369 4865 2419
Size: 248 702 1496 2542
Fragments arranged by size:
2542 1496 702 248
BsaWI w'CCGG_w
Cuts at: 741 955 1585 2177 2698 3376 3650 3797 4208
Size: 214 630 592 521 678 274 147 411
Cuts at: 4208 741
Size: 1521
Fragments arranged by size:
1521 678 630 592 521 411 274 214
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 808
Size: 30 4958
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 829 1233 1443 1681 449
Size: 380 404 210 238 3756
Fragments arranged by size:
3756 404 380 238 210
BseYI C'CCAG_C
Cuts at: 2413 3171 3748 4244 2413
Size: 758 577 496 3157
Fragments arranged by size:
3157 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3784 104
Size: 2426 46 1208 1308
Fragments arranged by size:
2426 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 547 626 1234 1724 1907 2002 2780 2970 3762
Size: 79 608 490 183 95 778 190 792
Cuts at: 3762 547
Size: 1773
Fragments arranged by size:
1773 792 778 608 490 190 183 95
79
Bsp1286I G_dGCh'C
Cuts at: 155 547 626 692 904 1234 1724 1907 2002
Size: 392 79 66 212 330 490 183 95
Cuts at: 2002 2613 2706 2780 2970 3032 3762 155
Size: 611 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 490 392 330 212 190
183 95 93 79 74 66 62
BspEI T'CCGG_A
Cuts at: 741 4208 741
Size: 3467 1521
BspHI T'CATG_A
Cuts at: 4164 4164
Size: 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1583 2038 2252 2554 2935 1583
Size: 455 214 302 381 3636
Fragments arranged by size:
3636 455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1395 3280 3334 4818 4983 50
Size: 1345 1885 54 1484 165 55
Fragments arranged by size:
1885 1484 1345 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1106 2900 1106
Size: 1794 3194
BsrFI r'CCGG_y
Cuts at: 2986 3167 3376 2986
Size: 181 209 4598
Fragments arranged by size:
4598 209 181
BssSI C'ACGA_G
Cuts at: 3259 3617 3259
Size: 358 4630
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 2365 1165
Size: 1200 3788
BstBI TT'CG_AA
Cuts at: 3349 3349
Size: 4988
BstYI r'GATC_y
Cuts at: 1 2013 2480 2503 2838 3084 4096 4182 4194
Size: 2012 467 23 335 246 1012 86 12
Cuts at: 4194 4540 1
Size: 346 449
Fragments arranged by size:
2012 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1658 1829 1658
Size: 171 4817
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 1635 2231 1635
Size: 596 4392
DraI TTT'AAA
Cuts at: 1987 1987
Size: 4988
DrdI GACnn_nn'nnGTC
Cuts at: 2694 3552 2694
Size: 858 4130
EaeI y'GGCC_r
Cuts at: 698 2573 2747 3138 3165 4510 698
Size: 1875 174 391 27 1345 1176
Fragments arranged by size:
1875 1345 1176 391 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 180 641 1145 1190 1385 1486 1552 1658 3011
Size: 461 504 45 195 101 66 106 1353
Cuts at: 3011 3221 180
Size: 210 1947
Fragments arranged by size:
1947 1353 504 461 210 195 106 101
66 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3196 3506 3652 4798 3196
Size: 310 146 1146 3386
Fragments arranged by size:
3386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 545 2000 545
Size: 1455 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 608 785 1002 2813 3245 3992 4260 520
Size: 88 177 217 1811 432 747 268 1248
Fragments arranged by size:
1811 1248 747 432 268 217 177 88
EcoO109I rG'GnC_Cy
Cuts at: 900 901 1098 1791 2127 900
Size: 1 197 693 336 3761
Fragments arranged by size:
3761 693 336 197 1
EcoRI G'AATT_C
Cuts at: 504 1992 504
Size: 1488 3500
EcoRV GAT'ATC
Cuts at: 1808 1808
Size: 4988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 1143 3007 240
Size: 903 1864 2221
Fragments arranged by size:
2221 1864 903
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4988
HaeII r_GCGC'y
Cuts at: 713 2422 2670 3692 4671 713
Size: 1709 248 1022 979 1030
Fragments arranged by size:
1709 1030 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 437 469 647 676 679 708 820 852 968
Size: 32 178 29 3 29 112 32 116
Cuts at: 968 1000 1228 1260 1516 1548 2830 2860 2862
Size: 32 228 32 256 32 1282 30 2
Cuts at: 2862 2892 437
Size: 30 2533
Fragments arranged by size:
2533 1282 256 228 178 116 112 32
32 32 32 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 2027 2027
Size: 4988
HindIII A'AGCT_T
Cuts at: 1846 1846
Size: 4988
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 2008 2008
Size: 4988
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 101 999 3659 3843 101
Size: 898 2660 184 1246
Fragments arranged by size:
2660 1246 898 184
MscI TGG'CCA
Cuts at: 700 2749 700
Size: 2049 2939
MslI CAynn'nnrTG
Cuts at: 559 577 1216 1876 1972 2977 3104 559
Size: 18 639 660 96 1005 127 2443
Fragments arranged by size:
2443 1005 660 639 127 96 18
MspA1I CmG'CkG
Cuts at: 2099 2413 2773 3786 4031 2099
Size: 314 360 1013 245 3056
Fragments arranged by size:
3056 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 4988
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NcoI C'CATG_G
Cuts at: 1658 1658
Size: 4988
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 4988
NsiI A_TGCA'T
Cuts at: 1764 1764
Size: 4988
NspI r_CATG'y
Cuts at: 825 2041 2541 3448 825
Size: 1216 500 907 2365
Fragments arranged by size:
2365 1216 907 500
PciI A'CATG_T
Cuts at: 821 3444 821
Size: 2623 2365
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PpuMI rG'GwC_Cy
Cuts at: 1098 1098
Size: 4988
PsiI TTA'TAA
Cuts at: 1301 1301
Size: 4988
PspOMI G'GGCC_C
Cuts at: 900 900
Size: 4988
PstI C_TGCA'G
Cuts at: 1580 2035 2720 1580
Size: 455 685 3848
Fragments arranged by size:
3848 685 455
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 2413 2773 2413
Size: 360 4628
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 547 2002 547
Size: 1455 3533
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 180 641 1385 3011 3221 180
Size: 461 744 1626 210 1947
Fragments arranged by size:
1947 1626 744 461 210
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SfcI C'TryA_G
Cuts at: 33 1576 2031 2716 3709 3900 33
Size: 1543 455 685 993 191 1121
Fragments arranged by size:
1543 1121 993 685 455 191
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 634 2010 634
Size: 1376 3612
SmlI C'TyrA_G
Cuts at: 329 1227 2143 3058 3550 3812 329
Size: 898 916 915 492 262 1505
Fragments arranged by size:
1505 916 915 898 492 262
SpeI A'CTAG_T
Cuts at: 67 138 67
Size: 71 4917
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
StuI AGG'CCT
Cuts at: 1429 1429
Size: 4988
StyI C'CwwG_G
Cuts at: 251 399 1658 2122 2476 251
Size: 148 1259 464 354 2763
Fragments arranged by size:
2763 1259 464 354 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 2571 2704 3163 3372 718
Size: 1853 133 459 209 2334
Fragments arranged by size:
2334 1853 459 209 133
TatI w'GTAC_w
Cuts at: 521 818 854 4503 521
Size: 297 36 3649 1006
Fragments arranged by size:
3649 1006 297 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 560 592 4505 4537 560
Size: 32 3913 32 1011
Fragments arranged by size:
3913 1011 32 32
Tth111I GACn'n_nGTC
Cuts at: 2785 2785
Size: 4988
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4988
XhoI C'TCGA_G
Cuts at: 329 329
Size: 4988
XmaI C'CCGG_G
Cuts at: 632 2008 632
Size: 1376 3612
XmnI GAAnn'nnTTC
Cuts at: 1207 1207
Size: 4988
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI