Restriction map for
pF1KB7857
(Circular) (Six-base) MAPSORT of: pF1KB7857.seq Check: 6306 from: 1 to: 4772
pF1KB7857 4772 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AatII G_ACGT'C
Cuts at: 1000 1000
Size: 4772
AccI GT'mk_AC
Cuts at: 1810 1810
Size: 4772
Acc65I G'GTAC_C
Cuts at: 1788 1788
Size: 4772
AclI AA'CG_TT
Cuts at: 4612 4612
Size: 4772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 972 1140 1308 2597 3029 3776 4044 972
Size: 168 168 1289 432 747 268 1700
Fragments arranged by size:
1700 1289 747 432 268 168 168
AflIII A'CryG_T
Cuts at: 1046 2124 3228 4205 1046
Size: 1078 1104 977 1613
Fragments arranged by size:
1613 1104 1078 977
AgeI A'CCGG_T
Cuts at: 3160 3160
Size: 4772
AleI CACnn'nnGTG
Cuts at: 520 1199 520
Size: 679 4093
AlwNI CAG_nnn'CTG
Cuts at: 744 1443 2077 3644 744
Size: 699 634 1567 1872
Fragments arranged by size:
1872 1567 699 634
ApaI G_GGCC'C
Cuts at: 180 1738 180
Size: 1558 3214
ApaLI G'TGCA_C
Cuts at: 701 1360 3542 701
Size: 659 2182 1931
Fragments arranged by size:
2182 1931 659
ApoI r'AATT_y
Cuts at: 1776 4125 4381 1776
Size: 2349 256 2167
Fragments arranged by size:
2349 2167 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
AvaI C'yCGr_G
Cuts at: 500 613 1731 1792 500
Size: 113 1118 61 3480
Fragments arranged by size:
3480 1118 113 61
BamHI G'GATC_C
Cuts at: 1610 1797 1610
Size: 187 4585
BanI G'GyrC_C
Cuts at: 448 487 1097 1501 1788 2202 2450 2485 448
Size: 39 610 404 287 414 248 35 2735
Fragments arranged by size:
2735 610 414 404 287 248 39 35
BanII G_rGCy'C
Cuts at: 158 180 284 827 916 1336 1721 1738 1786
Size: 22 104 543 89 420 385 17 48
Cuts at: 1786 2816 158
Size: 1030 2114
Fragments arranged by size:
2114 1030 543 420 385 104 89 48
22 17
BbeI G_GCGC'C
Cuts at: 1505 2206 2454 1505
Size: 701 248 3823
Fragments arranged by size:
3823 701 248
BbsI GAAGACnn'nnnn_
Cuts at: 426 1633 426
Size: 1207 3565
BbvCI CC'TCA_GC
Cuts at: 1174 1174
Size: 4772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 885 919 1473 1507 885
Size: 34 554 34 4150
Fragments arranged by size:
4150 554 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2670 3431 4772 2670
Size: 761 1341 2670
Fragments arranged by size:
2670 1341 761
BclI T'GATC_A
Cuts at: 2292 2292
Size: 4772
BglI GCCn_nnn'nGGC
Cuts at: 175 687 175
Size: 512 4260
BglII A'GATC_T
Cuts at: 2287 4324 2287
Size: 2037 2735
BlpI GC'TnA_GC
Cuts at: 1513 1884 1513
Size: 371 4401
Bme1580I G_kGCm'C
Cuts at: 180 705 1054 1364 1738 2397 2490 3546 180
Size: 525 349 310 374 659 93 1056 1406
Fragments arranged by size:
1406 1056 659 525 374 349 310 93
BmrI ACTGGGnnnn_n'
Cuts at: 287 546 966 1984 2065 2400 4544 287
Size: 259 420 1018 81 335 2144 515
Fragments arranged by size:
2144 1018 515 420 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 4772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 372 401 766 286
Size: 86 29 365 4292
Fragments arranged by size:
4292 365 86 29
Bpu10I CC'TnA_GC
Cuts at: 558 604 628 683 1174 558
Size: 46 24 55 491 4156
Fragments arranged by size:
4156 491 55 46 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1948 2827 3319 3617 910
Size: 1038 879 492 298 2065
Fragments arranged by size:
2065 1038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4476 4476
Size: 4772
BsaAI yAC'GTr
Cuts at: 2125 2125
Size: 4772
BsaBI GATnn'nnATC
Cuts at: 6 2286 4323 6
Size: 2280 2037 455
Fragments arranged by size:
2280 2037 455
BsaHI Gr'CG_yC
Cuts at: 620 679 997 1039 1206 1502 2203 2451 3153
Size: 59 318 42 167 296 701 248 702
Cuts at: 3153 4649 620
Size: 1496 743
Fragments arranged by size:
1496 743 702 701 318 296 248 167
59 42
BsaWI w'CCGG_w
Cuts at: 1961 2482 3160 3434 3581 3992 1961
Size: 521 678 274 147 411 2741
Fragments arranged by size:
2741 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 292 799 913 1074 1326 160
Size: 132 507 114 161 252 3606
Fragments arranged by size:
3606 507 252 161 132 114
BseYI C'CCAG_C
Cuts at: 225 660 843 862 1430 1737 2197 2955 3532
Size: 435 183 19 568 307 460 758 577
Cuts at: 3532 4028 225
Size: 496 969
Fragments arranged by size:
969 758 577 568 496 460 435 307
183 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 912 1429 1639 803
Size: 109 517 210 3936
Fragments arranged by size:
3936 517 210 109
BsiEI CG_ry'CG
Cuts at: 104 762 1118 1401 2314 2360 3568 104
Size: 658 356 283 913 46 1208 1308
Fragments arranged by size:
1308 1208 913 658 356 283 46
BsiHKAI G_wGCw'C
Cuts at: 705 916 1364 1786 2564 2754 3546 705
Size: 211 448 422 778 190 792 1931
Fragments arranged by size:
1931 792 778 448 422 211 190
BsiWI C'GTAC_G
Cuts at: 1273 1273
Size: 4772
BsmI GAATG_Cn'
Cuts at: 886 1526 886
Size: 640 4132
BsmBI CGTCTCn'nnnn_
Cuts at: 1030 1752 1030
Size: 722 4050
Bsp1286I G_dGCh'C
Cuts at: 158 180 284 705 827 916 1054 1336 1364
Size: 22 104 421 122 89 138 282 28
Cuts at: 1364 1721 1738 1786 2397 2490 2564 2754 2816
Size: 357 17 48 611 93 74 190 62
Cuts at: 2816 3546 158
Size: 730 1384
Fragments arranged by size:
1384 730 611 421 357 282 190 138
122 104 93 89 74 62 48 28
22 17
BspEI T'CCGG_A
Cuts at: 3992 3992
Size: 4772
BspHI T'CATG_A
Cuts at: 3948 3948
Size: 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1822 2036 2338 2719 1822
Size: 214 302 381 3875
Fragments arranged by size:
3875 381 302 214
BsrBI CCG'CTC
Cuts at: 50 687 954 1458 3064 3118 4602 4767 50
Size: 637 267 504 1606 54 1484 165 55
Fragments arranged by size:
1606 1484 637 504 267 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2684 2684
Size: 4772
BsrFI r'CCGG_y
Cuts at: 445 754 1520 1618 2770 2951 3160 445
Size: 309 766 98 1152 181 209 2057
Fragments arranged by size:
2057 1152 766 309 209 181 98
BssHII G'CGCG_C
Cuts at: 1304 1304
Size: 4772
BssSI C'ACGA_G
Cuts at: 248 392 1267 3043 3401 248
Size: 144 875 1776 358 1619
Fragments arranged by size:
1776 1619 875 358 144
BstAPI GCAn_nnn'nTGC
Cuts at: 657 890 2149 657
Size: 233 1259 3280
Fragments arranged by size:
3280 1259 233
BstBI TT'CG_AA
Cuts at: 878 3133 878
Size: 2255 2517
BstXI CCAn_nnnn'nTGG
Cuts at: 1201 1201
Size: 4772
BstYI r'GATC_y
Cuts at: 1 1190 1610 1797 2264 2287 2622 2868 3880
Size: 1189 420 187 467 23 335 246 1012
Cuts at: 3880 3966 3978 4324 1
Size: 86 12 346 449
Fragments arranged by size:
1189 1012 467 449 420 346 335 246
187 86 23 12
BtgI C'CryG_G
Cuts at: 107 515 1161 107
Size: 408 646 3718
Fragments arranged by size:
3718 646 408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2722 2903 3066 2722
Size: 181 163 4428
Fragments arranged by size:
4428 181 163
BtsI GCAGTG_nn'
Cuts at: 570 1567 2015 570
Size: 997 448 3327
Fragments arranged by size:
3327 997 448
DraI TTT'AAA
Cuts at: 1771 1771
Size: 4772
DraIII CAC_nnn'GTG
Cuts at: 1228 1228
Size: 4772
DrdI GACnn_nn'nnGTC
Cuts at: 2478 3336 2478
Size: 858 3914
EaeI y'GGCC_r
Cuts at: 388 816 1115 1230 1398 1566 2357 2531 2922
Size: 428 299 115 168 168 791 174 391
Cuts at: 2922 2949 4294 388
Size: 27 1345 866
Fragments arranged by size:
1345 866 791 428 391 299 174 168
168 115 27
EagI C'GGCC_G
Cuts at: 1115 1398 2357 1115
Size: 283 959 3530
Fragments arranged by size:
3530 959 283
EarI CTCTTCn'nnn_
Cuts at: 860 904 1600 2795 3005 860
Size: 44 696 1195 210 2627
Fragments arranged by size:
2627 1195 696 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2980 3290 3436 4582 2980
Size: 310 146 1146 3170
Fragments arranged by size:
3170 1146 310 146
EcoICRI GAG'CTC
Cuts at: 914 1784 914
Size: 870 3902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 372 401 766 972 1140 1308 2597 3029
Size: 86 29 365 206 168 168 1289 432
Cuts at: 3029 3776 4044 286
Size: 747 268 1014
Fragments arranged by size:
1289 1014 747 432 365 268 206 168
168 86 29
EcoNI CCTnn'n_nnAGG
Cuts at: 330 330
Size: 4772
EcoO109I rG'GnC_Cy
Cuts at: 176 177 711 741 1911 176
Size: 1 534 30 1170 3037
Fragments arranged by size:
3037 1170 534 30 1
EcoRI G'AATT_C
Cuts at: 1776 1776
Size: 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 4772
FspI TGC'GCA
Cuts at: 1221 2553 1221
Size: 1332 3440
HaeII r_GCGC'y
Cuts at: 737 1505 1587 2206 2454 3476 4455 737
Size: 768 82 619 248 1022 979 1054
Fragments arranged by size:
1054 1022 979 768 619 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2614 2644 2646 2676 2614
Size: 30 2 30 4710
Fragments arranged by size:
4710 30 30 2
HincII GTy'rAC
Cuts at: 1811 1811
Size: 4772
KasI G'GCGC_C
Cuts at: 1501 2202 2450 1501
Size: 701 248 3823
Fragments arranged by size:
3823 701 248
KpnI G_GTAC'C
Cuts at: 1792 1792
Size: 4772
MluI A'CGCG_T
Cuts at: 1046 4205 1046
Size: 3159 1613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 285 941 1193 1445 3443 3627 285
Size: 656 252 252 1998 184 1430
Fragments arranged by size:
1998 1430 656 252 252 184
MscI TGG'CCA
Cuts at: 390 818 2533 390
Size: 428 1715 2629
Fragments arranged by size:
2629 1715 428
MslI CAynn'nnrTG
Cuts at: 520 1199 2761 2888 520
Size: 679 1562 127 2404
Fragments arranged by size:
2404 1562 679 127
MspA1I CmG'CkG
Cuts at: 1031 1133 1469 1759 1883 2197 2557 3570 3815
Size: 102 336 290 124 314 360 1013 245
Cuts at: 3815 1031
Size: 1988
Fragments arranged by size:
1988 1013 360 336 314 290 245 124
102
NaeI GCC'GGC
Cuts at: 756 2953 756
Size: 2197 2575
NarI GG'CG_CC
Cuts at: 1502 2203 2451 1502
Size: 701 248 3823
Fragments arranged by size:
3823 701 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4772
NgoMIV G'CCGG_C
Cuts at: 754 2951 754
Size: 2197 2575
NotI GC'GGCC_GC
Cuts at: 1398 1398
Size: 4772
NspI r_CATG'y
Cuts at: 655 1825 2325 3232 655
Size: 1170 500 907 2195
Fragments arranged by size:
2195 1170 907 500
PasI CC'CwG_GG
Cuts at: 284 715 284
Size: 431 4341
PciI A'CATG_T
Cuts at: 3228 3228
Size: 4772
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 4772
PfoI T'CCnGG_A
Cuts at: 158 316 349 158
Size: 158 33 4581
Fragments arranged by size:
4581 158 33
PmeI GTTT'AAAC
Cuts at: 1771 1771
Size: 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4740
PpuMI rG'GwC_Cy
Cuts at: 711 741 711
Size: 30 4742
PspOMI G'GGCC_C
Cuts at: 176 1734 176
Size: 1558 3214
PstI C_TGCA'G
Cuts at: 476 1819 2504 476
Size: 1343 685 2744
Fragments arranged by size:
2744 1343 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4772
PvuII CAG'CTG
Cuts at: 2197 2557 2197
Size: 360 4412
RsrII CG'GwC_CG
Cuts at: 2967 2967
Size: 4772
SacI G_AGCT'C
Cuts at: 916 1786 916
Size: 870 3902
SalI G'TCGA_C
Cuts at: 1809 1809
Size: 4772
SapI GCTCTTCn'nnn_
Cuts at: 860 904 2795 3005 860
Size: 44 1891 210 2627
Fragments arranged by size:
2627 1891 210 44
SbfI CC_TGCA'GG
Cuts at: 1819 1819
Size: 4772
ScaI AGT'ACT
Cuts at: 4289 4289
Size: 4772
SfcI C'TryA_G
Cuts at: 33 327 472 1815 2500 3493 3684 33
Size: 294 145 1343 685 993 191 1121
Fragments arranged by size:
1343 1121 993 685 294 191 145
SfoI GGC'GCC
Cuts at: 1503 2204 2452 1503
Size: 701 248 3823
Fragments arranged by size:
3823 701 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
SgrAI Cr'CCGG_yG
Cuts at: 1520 1520
Size: 4772
SmaI CCC'GGG
Cuts at: 1794 1794
Size: 4772
SmlI C'TyrA_G
Cuts at: 925 1927 2842 3334 3596 925
Size: 1002 915 492 262 2101
Fragments arranged by size:
2101 1002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4772
SphI G_CATG'C
Cuts at: 655 1825 2325 655
Size: 1170 500 3102
Fragments arranged by size:
3102 1170 500
StuI AGG'CCT
Cuts at: 609 985 1069 1153 1489 609
Size: 376 84 84 336 3892
Fragments arranged by size:
3892 376 336 84 84
StyI C'CwwG_G
Cuts at: 107 1496 1906 2260 107
Size: 1389 410 354 2619
Fragments arranged by size:
2619 1389 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 955 1444 2355 2488 2947 3156 769
Size: 186 489 911 133 459 209 2385
Fragments arranged by size:
2385 911 489 459 209 186 133
TatI w'GTAC_w
Cuts at: 131 4287 131
Size: 4156 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 383 415 695 727 4289 4321 383
Size: 32 280 32 3562 32 834
Fragments arranged by size:
3562 834 280 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2569 2569
Size: 4772
XbaI T'CTAG_A
Cuts at: 1803 1803
Size: 4772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 268 2267 268
Size: 1999 2773
XmaI C'CCGG_G
Cuts at: 1792 1792
Size: 4772
XmnI GAAnn'nnTTC
Cuts at: 874 1126 1294 1378 1546 874
Size: 252 168 84 168 4100
Fragments arranged by size:
4100 252 168 168 84
ZraI GAC'GTC
Cuts at: 998 998
Size: 4772
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI