Restriction map for
pF1KB7861
(Circular) (Six-base) MAPSORT of: pF1KB7861.seq Check: 9952 from: 1 to: 4775
pF1KB7861 4775 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1210 1210
Size: 4775
AccI GT'mk_AC
Cuts at: 1813 1813
Size: 4775
Acc65I G'GTAC_C
Cuts at: 219 1791 219
Size: 1572 3203
AclI AA'CG_TT
Cuts at: 4615 4615
Size: 4775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 585 753 1041 1209 1461 1545 1629 2600
Size: 376 168 288 168 252 84 84 971
Cuts at: 2600 3032 3779 4047 209
Size: 432 747 268 937
Fragments arranged by size:
971 937 747 432 376 288 268 252
168 168 84 84
AfeI AGC'GCT
Cuts at: 449 1138 1306 449
Size: 689 168 3918
Fragments arranged by size:
3918 689 168
AflIII A'CryG_T
Cuts at: 1283 2127 3231 4208 1283
Size: 844 1104 977 1850
Fragments arranged by size:
1850 1104 977 844
AgeI A'CCGG_T
Cuts at: 1031 3163 1031
Size: 2132 2643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 4775
AlwNI CAG_nnn'CTG
Cuts at: 2080 3647 2080
Size: 1567 3208
ApaI G_GGCC'C
Cuts at: 621 1697 621
Size: 1076 3699
ApaLI G'TGCA_C
Cuts at: 696 3545 696
Size: 2849 1926
ApoI r'AATT_y
Cuts at: 1779 4128 4384 1779
Size: 2349 256 2170
Fragments arranged by size:
2349 2170 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4775
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
AvaI C'yCGr_G
Cuts at: 410 509 648 1459 1795 410
Size: 99 139 811 336 3390
Fragments arranged by size:
3390 811 336 139 99
BamHI G'GATC_C
Cuts at: 395 1800 395
Size: 1405 3370
BanI G'GyrC_C
Cuts at: 219 313 327 970 1342 1354 1791 2205 2453
Size: 94 14 643 372 12 437 414 248
Cuts at: 2453 2488 219
Size: 35 2506
Fragments arranged by size:
2506 643 437 414 372 248 94 35
14 12
BanII G_rGCy'C
Cuts at: 266 309 381 621 655 841 926 1405 1697
Size: 43 72 240 34 186 85 479 292
Cuts at: 1697 1743 1789 2819 266
Size: 46 46 1030 2222
Fragments arranged by size:
2222 1030 479 292 240 186 85 72
46 46 43 34
BbeI G_GCGC'C
Cuts at: 974 1358 2209 2457 974
Size: 384 851 248 3292
Fragments arranged by size:
3292 851 384 248
BciVI GTATCCnnnnn_n'
Cuts at: 2673 3434 4775 2673
Size: 761 1341 2673
Fragments arranged by size:
2673 1341 761
BclI T'GATC_A
Cuts at: 2295 2295
Size: 4775
BglII A'GATC_T
Cuts at: 2290 4327 2290
Size: 2037 2738
BlpI GC'TnA_GC
Cuts at: 872 1887 872
Size: 1015 3760
Bme1580I G_kGCm'C
Cuts at: 330 621 700 1345 1375 1459 1697 2400 2493
Size: 291 79 645 30 84 238 703 93
Cuts at: 2493 3549 330
Size: 1056 1556
Fragments arranged by size:
1556 1056 703 645 291 238 93 84
79 30
BmrI ACTGGGnnnn_n'
Cuts at: 598 905 1445 1987 2068 2403 4547 598
Size: 307 540 542 81 335 2144 826
Fragments arranged by size:
2144 826 542 540 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 294 3142 294
Size: 2848 1927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 639
Size: 4775
Bpu10I CC'TnA_GC
Cuts at: 840 840
Size: 4775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 608 1951 2830 3322 3620 293
Size: 315 1343 879 492 298 1448
Fragments arranged by size:
1448 1343 879 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 422 4479 422
Size: 4057 718
BsaAI yAC'GTr
Cuts at: 1284 2128 1284
Size: 844 3931
BsaBI GATnn'nnATC
Cuts at: 6 2289 4326 6
Size: 2283 2037 455
Fragments arranged by size:
2283 2037 455
BsaHI Gr'CG_yC
Cuts at: 800 971 1355 2206 2454 3156 4652 800
Size: 171 384 851 248 702 1496 923
Fragments arranged by size:
1496 923 851 702 384 248 171
BsaWI w'CCGG_w
Cuts at: 1031 1267 1964 2485 3163 3437 3584 3995 1031
Size: 236 697 521 678 274 147 411 1811
Fragments arranged by size:
1811 697 678 521 411 274 236 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 529 973 976 1003 442
Size: 87 444 3 27 4214
Fragments arranged by size:
4214 444 87 27 3
BseYI C'CCAG_C
Cuts at: 207 323 1719 2200 2958 3535 4031 207
Size: 116 1396 481 758 577 496 951
Fragments arranged by size:
1396 951 758 577 496 481 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 748 1104 1648 232
Size: 516 356 544 3359
Fragments arranged by size:
3359 544 516 356
BsiEI CG_ry'CG
Cuts at: 104 834 1146 1293 2317 2363 3571 104
Size: 730 312 147 1024 46 1208 1308
Fragments arranged by size:
1308 1208 1024 730 312 147 46
BsiHKAI G_wGCw'C
Cuts at: 700 841 1405 1789 2567 2757 3549 700
Size: 141 564 384 778 190 792 1926
Fragments arranged by size:
1926 792 778 564 384 190 141
Bsp1286I G_dGCh'C
Cuts at: 266 309 330 381 621 655 700 841 926
Size: 43 21 51 240 34 45 141 85
Cuts at: 926 1345 1375 1405 1459 1697 1743 1789 2400
Size: 419 30 30 54 238 46 46 611
Cuts at: 2400 2493 2567 2757 2819 3549 266
Size: 93 74 190 62 730 1492
Fragments arranged by size:
1492 730 611 419 240 238 190 141
93 85 74 62 54 51 46 46
45 43 34 30 30 21
BspEI T'CCGG_A
Cuts at: 3995 3995
Size: 4775
BspHI T'CATG_A
Cuts at: 3951 3951
Size: 4775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1670 1825 2039 2341 2722 1210
Size: 460 155 214 302 381 3263
Fragments arranged by size:
3263 460 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 1319 1762 3067 3121 4605 4770 50
Size: 1269 443 1305 54 1484 165 55
Fragments arranged by size:
1484 1305 1269 443 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2687 2687
Size: 4775
BsrFI r'CCGG_y
Cuts at: 431 659 846 867 1031 1085 1649 1667 2773
Size: 228 187 21 164 54 564 18 1106
Cuts at: 2773 2954 3163 431
Size: 181 209 2043
Fragments arranged by size:
2043 1106 564 228 209 187 181 164
54 21 18
BssHII G'CGCG_C
Cuts at: 812 812
Size: 4775
BssSI C'ACGA_G
Cuts at: 1672 3046 3404 1672
Size: 1374 358 3043
Fragments arranged by size:
3043 1374 358
BstAPI GCAn_nnn'nTGC
Cuts at: 726 1127 2152 726
Size: 401 1025 3349
Fragments arranged by size:
3349 1025 401
BstBI TT'CG_AA
Cuts at: 3136 3136
Size: 4775
BstEII G'GTnAC_C
Cuts at: 1329 1329
Size: 4775
BstXI CCAn_nnnn'nTGG
Cuts at: 1704 1704
Size: 4775
BstYI r'GATC_y
Cuts at: 1 395 1679 1800 2267 2290 2625 2871 3883
Size: 394 1284 121 467 23 335 246 1012
Cuts at: 3883 3969 3981 4327 1
Size: 86 12 346 449
Fragments arranged by size:
1284 1012 467 449 394 346 335 246
121 86 23 12
BtgI C'CryG_G
Cuts at: 740 917 1602 740
Size: 177 685 3913
Fragments arranged by size:
3913 685 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 275 1482 2725 2906 3069 275
Size: 1207 1243 181 163 1981
Fragments arranged by size:
1981 1243 1207 181 163
BtsI GCAGTG_nn'
Cuts at: 244 455 814 1048 1536 2018 244
Size: 211 359 234 488 482 3001
Fragments arranged by size:
3001 488 482 359 234 211
DraI TTT'AAA
Cuts at: 1774 1774
Size: 4775
DraIII CAC_nnn'GTG
Cuts at: 1077 1077
Size: 4775
DrdI GACnn_nn'nnGTC
Cuts at: 2481 3339 2481
Size: 858 3917
EaeI y'GGCC_r
Cuts at: 831 848 1143 1706 1745 2360 2534 2925 2952
Size: 17 295 563 39 615 174 391 27
Cuts at: 2952 4297 831
Size: 1345 1309
Fragments arranged by size:
1345 1309 615 563 391 295 174 39
27 17
EagI C'GGCC_G
Cuts at: 831 1143 2360 831
Size: 312 1217 3246
Fragments arranged by size:
3246 1217 312
EarI CTCTTCn'nnn_
Cuts at: 263 367 1018 1213 2798 3008 263
Size: 104 651 195 1585 210 2030
Fragments arranged by size:
2030 1585 651 210 195 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 137 389 890 2983 3293 3439 4585 137
Size: 252 501 2093 310 146 1146 327
Fragments arranged by size:
2093 1146 501 327 310 252 146
EcoICRI GAG'CTC
Cuts at: 839 1403 1787 839
Size: 564 384 3827
Fragments arranged by size:
3827 564 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 585 639 753 1041 1209 1461 1545 1629
Size: 376 54 114 288 168 252 84 84
Cuts at: 1629 2600 3032 3779 4047 209
Size: 971 432 747 268 937
Fragments arranged by size:
971 937 747 432 376 288 268 252
168 114 84 84 54
EcoNI CCTnn'n_nnAGG
Cuts at: 603 603
Size: 4775
EcoO109I rG'GnC_Cy
Cuts at: 618 993 1701 1914 618
Size: 375 708 213 3479
Fragments arranged by size:
3479 708 375 213
EcoRI G'AATT_C
Cuts at: 1779 1779
Size: 4775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 4775
FseI GG_CCGG'CC
Cuts at: 850 850
Size: 4775
FspI TGC'GCA
Cuts at: 2556 2556
Size: 4775
HaeII r_GCGC'y
Cuts at: 451 974 1140 1308 1358 1411 1656 2209 2457
Size: 523 166 168 50 53 245 553 248
Cuts at: 2457 3479 4458 451
Size: 1022 979 768
Fragments arranged by size:
1022 979 768 553 523 248 245 168
166 53 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 2617 2647 2649 2679 274
Size: 32 2311 30 2 30 2370
Fragments arranged by size:
2370 2311 32 30 30 2
HincII GTy'rAC
Cuts at: 1814 1814
Size: 4775
KasI G'GCGC_C
Cuts at: 970 1354 2205 2453 970
Size: 384 851 248 3292
Fragments arranged by size:
3292 851 384 248
KpnI G_GTAC'C
Cuts at: 223 1795 223
Size: 1572 3203
MluI A'CGCG_T
Cuts at: 4208 4208
Size: 4775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1514 1682 3446 3630 1514
Size: 168 1764 184 2659
Fragments arranged by size:
2659 1764 184 168
MscI TGG'CCA
Cuts at: 1708 1747 2536 1708
Size: 39 789 3947
Fragments arranged by size:
3947 789 39
MslI CAynn'nnrTG
Cuts at: 256 1714 2764 2891 256
Size: 1458 1050 127 2140
Fragments arranged by size:
2140 1458 1050 127
MspA1I CmG'CkG
Cuts at: 1061 1295 1886 2200 2560 3573 3818 1061
Size: 234 591 314 360 1013 245 2018
Fragments arranged by size:
2018 1013 591 360 314 245 234
NaeI GCC'GGC
Cuts at: 661 848 869 1651 2956 661
Size: 187 21 782 1305 2480
Fragments arranged by size:
2480 1305 782 187 21
NarI GG'CG_CC
Cuts at: 971 1355 2206 2454 971
Size: 384 851 248 3292
Fragments arranged by size:
3292 851 384 248
NgoMIV G'CCGG_C
Cuts at: 659 846 867 1649 2954 659
Size: 187 21 782 1305 2480
Fragments arranged by size:
2480 1305 782 187 21
NspI r_CATG'y
Cuts at: 1828 2328 3235 1828
Size: 500 907 3368
Fragments arranged by size:
3368 907 500
PciI A'CATG_T
Cuts at: 3231 3231
Size: 4775
PflMI CCAn_nnn'nTGG
Cuts at: 175 175
Size: 4775
PfoI T'CCnGG_A
Cuts at: 131 941 131
Size: 810 3965
PmeI GTTT'AAAC
Cuts at: 1774 1774
Size: 4775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4743
PshAI GACnn'nnGTC
Cuts at: 911 911
Size: 4775
PspOMI G'GGCC_C
Cuts at: 617 1693 617
Size: 1076 3699
PstI C_TGCA'G
Cuts at: 117 447 569 1822 2507 117
Size: 330 122 1253 685 2385
Fragments arranged by size:
2385 1253 685 330 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4775
PvuII CAG'CTG
Cuts at: 1061 2200 2560 1061
Size: 1139 360 3276
Fragments arranged by size:
3276 1139 360
RsrII CG'GwC_CG
Cuts at: 2970 2970
Size: 4775
SacI G_AGCT'C
Cuts at: 841 1405 1789 841
Size: 564 384 3827
Fragments arranged by size:
3827 564 384
SalI G'TCGA_C
Cuts at: 1812 1812
Size: 4775
SapI GCTCTTCn'nnn_
Cuts at: 263 1018 1213 2798 3008 263
Size: 755 195 1585 210 2030
Fragments arranged by size:
2030 1585 755 210 195
SbfI CC_TGCA'GG
Cuts at: 1822 1822
Size: 4775
ScaI AGT'ACT
Cuts at: 4292 4292
Size: 4775
SexAI A'CCwGG_T
Cuts at: 1073 1073
Size: 4775
SfcI C'TryA_G
Cuts at: 33 113 443 552 565 1818 2503 3496 3687
Size: 80 330 109 13 1253 685 993 191
Cuts at: 3687 33
Size: 1121
Fragments arranged by size:
1253 1121 993 685 330 191 109 80
13
SfoI GGC'GCC
Cuts at: 972 1356 2207 2455 972
Size: 384 851 248 3292
Fragments arranged by size:
3292 851 384 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
SgrAI Cr'CCGG_yG
Cuts at: 1085 1085
Size: 4775
SmaI CCC'GGG
Cuts at: 511 1797 511
Size: 1286 3489
SmlI C'TyrA_G
Cuts at: 308 623 1930 2845 3337 3599 308
Size: 315 1307 915 492 262 1484
Fragments arranged by size:
1484 1307 915 492 315 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4775
SphI G_CATG'C
Cuts at: 1828 2328 1828
Size: 500 4275
StuI AGG'CCT
Cuts at: 609 646 609
Size: 37 4738
StyI C'CwwG_G
Cuts at: 750 1697 1909 2263 750
Size: 947 212 354 3262
Fragments arranged by size:
3262 947 354 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 1348 1360 1432 1444 1612 2358 2491 2950
Size: 1194 12 72 12 168 746 133 459
Cuts at: 2950 3159 154
Size: 209 1770
Fragments arranged by size:
1770 1194 746 459 209 168 133 72
12 12
TatI w'GTAC_w
Cuts at: 4290 4290
Size: 4775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4292 4324 4292
Size: 32 4743
Tth111I GACn'n_nGTC
Cuts at: 1327 2572 1327
Size: 1245 3530
XbaI T'CTAG_A
Cuts at: 1806 1806
Size: 4775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1716 2270 1716
Size: 554 4221
XmaI C'CCGG_G
Cuts at: 509 1795 509
Size: 1286 3489
XmnI GAAnn'nnTTC
Cuts at: 766 1138 1222 766
Size: 372 84 4319
Fragments arranged by size:
4319 372 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SnaBI SrfI SspI
SwaI XhoI ZraI