Restriction map for
pF1KB7863
(Circular) (Six-base) MAPSORT of: pF1KB7863.seq Check: 4845 from: 1 to: 4775
pF1KB7863 4775 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 746 1813 746
Size: 1067 3708
Acc65I G'GTAC_C
Cuts at: 1791 1791
Size: 4775
AclI AA'CG_TT
Cuts at: 4615 4615
Size: 4775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 555 708 808 1373 1545 2600 3032 3779
Size: 101 153 100 565 172 1055 432 747
Cuts at: 3779 4047 454
Size: 268 1182
Fragments arranged by size:
1182 1055 747 565 432 268 172 153
101 100
AflII C'TTAA_G
Cuts at: 589 589
Size: 4775
AflIII A'CryG_T
Cuts at: 1648 2127 3231 4208 1648
Size: 479 1104 977 2215
Fragments arranged by size:
2215 1104 977 479
AgeI A'CCGG_T
Cuts at: 3163 3163
Size: 4775
AlwNI CAG_nnn'CTG
Cuts at: 118 2080 3647 118
Size: 1962 1567 1246
Fragments arranged by size:
1962 1567 1246
ApaLI G'TGCA_C
Cuts at: 3545 3545
Size: 4775
ApoI r'AATT_y
Cuts at: 1499 1667 1763 1779 4128 4384 1499
Size: 168 96 16 2349 256 1890
Fragments arranged by size:
2349 1890 256 168 96 16
AseI AT'TA_AT
Cuts at: 20 809 20
Size: 789 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
AvaI C'yCGr_G
Cuts at: 1152 1795 1152
Size: 643 4132
BamHI G'GATC_C
Cuts at: 1800 1800
Size: 4775
BanI G'GyrC_C
Cuts at: 1791 2205 2453 2488 1791
Size: 414 248 35 4078
Fragments arranged by size:
4078 414 248 35
BanII G_rGCy'C
Cuts at: 1789 2819 1789
Size: 1030 3745
BbeI G_GCGC'C
Cuts at: 2209 2457 2209
Size: 248 4527
BbsI GAAGACnn'nnnn_
Cuts at: 561 1758 561
Size: 1197 3578
BciVI GTATCCnnnnn_n'
Cuts at: 500 1409 2673 3434 4775 500
Size: 909 1264 761 1341 500
Fragments arranged by size:
1341 1264 909 761 500
BclI T'GATC_A
Cuts at: 2295 2295
Size: 4775
BfrBI ATG'CAT
Cuts at: 1167 1167
Size: 4775
BglII A'GATC_T
Cuts at: 2290 4327 2290
Size: 2037 2738
BlpI GC'TnA_GC
Cuts at: 1887 1887
Size: 4775
Bme1580I G_kGCm'C
Cuts at: 1440 2400 2493 3549 1440
Size: 960 93 1056 2666
Fragments arranged by size:
2666 1056 960 93
BmgBI CAC'GTC
Cuts at: 315 315
Size: 4775
BmrI ACTGGGnnnn_n'
Cuts at: 148 365 450 1025 1987 2068 2403 4547 148
Size: 217 85 575 962 81 335 2144 376
Fragments arranged by size:
2144 962 575 376 335 217 85 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 693 874 3142 442
Size: 251 181 2268 2075
Fragments arranged by size:
2268 2075 251 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1121 1289 1541 1625 343
Size: 778 168 252 84 3493
Fragments arranged by size:
3493 778 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1951 2830 3322 3620 1951
Size: 879 492 298 3106
Fragments arranged by size:
3106 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4479 4479
Size: 4775
BsaAI yAC'GTr
Cuts at: 600 1649 2128 600
Size: 1049 479 3247
Fragments arranged by size:
3247 1049 479
BsaBI GATnn'nnATC
Cuts at: 6 218 2289 4326 6
Size: 212 2071 2037 455
Fragments arranged by size:
2071 2037 455 212
BsaHI Gr'CG_yC
Cuts at: 2206 2454 3156 4652 2206
Size: 248 702 1496 2329
Fragments arranged by size:
2329 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1964 2485 3163 3437 3584 3995 1964
Size: 521 678 274 147 411 2744
Fragments arranged by size:
2744 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 434 283
Size: 151 4624
BseYI C'CCAG_C
Cuts at: 981 2200 2958 3535 4031 981
Size: 1219 758 577 496 1725
Fragments arranged by size:
1725 1219 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2317 2363 3571 104
Size: 2213 46 1208 1308
Fragments arranged by size:
2213 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1789 2567 2757 3549 1789
Size: 778 190 792 3015
Fragments arranged by size:
3015 792 778 190
BsmI GAATG_Cn'
Cuts at: 1627 1627
Size: 4775
Bsp1286I G_dGCh'C
Cuts at: 1440 1789 2400 2493 2567 2757 2819 3549 1440
Size: 349 611 93 74 190 62 730 2666
Fragments arranged by size:
2666 730 611 349 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3995 3995
Size: 4775
BspHI T'CATG_A
Cuts at: 165 627 1671 3951 165
Size: 462 1044 2280 989
Fragments arranged by size:
2280 1044 989 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1825 2039 2341 2722 1825
Size: 214 302 381 3878
Fragments arranged by size:
3878 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3067 3121 4605 4770 50
Size: 3017 54 1484 165 55
Fragments arranged by size:
3017 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 562 1720 2687 562
Size: 1158 967 2650
Fragments arranged by size:
2650 1158 967
BsrFI r'CCGG_y
Cuts at: 2773 2954 3163 2773
Size: 181 209 4385
Fragments arranged by size:
4385 209 181
BssSI C'ACGA_G
Cuts at: 3046 3404 3046
Size: 358 4417
BstAPI GCAn_nnn'nTGC
Cuts at: 2152 2152
Size: 4775
BstBI TT'CG_AA
Cuts at: 1749 3136 1749
Size: 1387 3388
BstYI r'GATC_y
Cuts at: 1 1800 2267 2290 2625 2871 3883 3969 3981
Size: 1799 467 23 335 246 1012 86 12
Cuts at: 3981 4327 1
Size: 346 449
Fragments arranged by size:
1799 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 747 747
Size: 4775
BtgI C'CryG_G
Cuts at: 107 129 107
Size: 22 4753
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2725 2906 3069 2725
Size: 181 163 4431
Fragments arranged by size:
4431 181 163
BtsI GCAGTG_nn'
Cuts at: 2018 2018
Size: 4775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1614 1649 1614
Size: 35 4740
DraI TTT'AAA
Cuts at: 1774 1774
Size: 4775
DrdI GACnn_nn'nnGTC
Cuts at: 2481 3339 2481
Size: 858 3917
EaeI y'GGCC_r
Cuts at: 341 2360 2534 2925 2952 4297 341
Size: 2019 174 391 27 1345 819
Fragments arranged by size:
2019 1345 819 391 174 27
EagI C'GGCC_G
Cuts at: 2360 2360
Size: 4775
EarI CTCTTCn'nnn_
Cuts at: 392 1349 1685 2798 3008 392
Size: 957 336 1113 210 2159
Fragments arranged by size:
2159 1113 957 336 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2983 3293 3439 4585 2983
Size: 310 146 1146 3173
Fragments arranged by size:
3173 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1787 1787
Size: 4775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 454 555 708 808 1121 1289 1373 1541
Size: 111 101 153 100 313 168 84 168
Cuts at: 1541 1545 1625 2600 3032 3779 4047 343
Size: 4 80 975 432 747 268 1071
Fragments arranged by size:
1071 975 747 432 313 268 168 168
153 111 101 100 84 80 4
EcoO109I rG'GnC_Cy
Cuts at: 150 1914 150
Size: 1764 3011
EcoRI G'AATT_C
Cuts at: 1667 1779 1667
Size: 112 4663
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 673 2794 673
Size: 2121 2654
FspI TGC'GCA
Cuts at: 2556 2556
Size: 4775
HaeII r_GCGC'y
Cuts at: 2209 2457 3479 4458 2209
Size: 248 1022 979 2526
Fragments arranged by size:
2526 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 2617 2647 2649 2679 344
Size: 32 2241 30 2 30 2440
Fragments arranged by size:
2440 2241 32 30 30 2
HincII GTy'rAC
Cuts at: 1814 1814
Size: 4775
HindIII A'AGCT_T
Cuts at: 1724 1724
Size: 4775
KasI G'GCGC_C
Cuts at: 2205 2453 2205
Size: 248 4527
KpnI G_GTAC'C
Cuts at: 1795 1795
Size: 4775
MfeI C'AATT_G
Cuts at: 511 511
Size: 4775
MluI A'CGCG_T
Cuts at: 4208 4208
Size: 4775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 1091 3446 3630 368
Size: 723 2355 184 1513
Fragments arranged by size:
2355 1513 723 184
MscI TGG'CCA
Cuts at: 343 2536 343
Size: 2193 2582
MslI CAynn'nnrTG
Cuts at: 780 1032 2764 2891 780
Size: 252 1732 127 2664
Fragments arranged by size:
2664 1732 252 127
MspA1I CmG'CkG
Cuts at: 115 1886 2200 2560 3573 3818 115
Size: 1771 314 360 1013 245 1072
Fragments arranged by size:
1771 1072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2956 2956
Size: 4775
NarI GG'CG_CC
Cuts at: 2206 2454 2206
Size: 248 4527
NcoI C'CATG_G
Cuts at: 107 129 107
Size: 22 4753
NdeI CA'TA_TG
Cuts at: 833 1029 833
Size: 196 4579
NgoMIV G'CCGG_C
Cuts at: 2954 2954
Size: 4775
NsiI A_TGCA'T
Cuts at: 1169 1169
Size: 4775
NspI r_CATG'y
Cuts at: 1005 1171 1425 1509 1828 2328 3235 1005
Size: 166 254 84 319 500 907 2545
Fragments arranged by size:
2545 907 500 319 254 166 84
PciI A'CATG_T
Cuts at: 3231 3231
Size: 4775
PmeI GTTT'AAAC
Cuts at: 1774 1774
Size: 4775
PmlI CAC'GTG
Cuts at: 600 1649 600
Size: 1049 3726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4743
PpuMI rG'GwC_Cy
Cuts at: 150 150
Size: 4775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 597 629 597
Size: 32 4743
PstI C_TGCA'G
Cuts at: 1822 2507 1822
Size: 685 4090
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4775
PvuII CAG'CTG
Cuts at: 115 2200 2560 115
Size: 2085 360 2330
Fragments arranged by size:
2330 2085 360
RsrII CG'GwC_CG
Cuts at: 2970 2970
Size: 4775
SacI G_AGCT'C
Cuts at: 1789 1789
Size: 4775
SalI G'TCGA_C
Cuts at: 1812 1812
Size: 4775
SanDI GG'GwC_CC
Cuts at: 150 150
Size: 4775
SapI GCTCTTCn'nnn_
Cuts at: 2798 3008 2798
Size: 210 4565
SbfI CC_TGCA'GG
Cuts at: 1822 1822
Size: 4775
ScaI AGT'ACT
Cuts at: 1150 4292 1150
Size: 3142 1633
SfcI C'TryA_G
Cuts at: 33 306 972 1818 2503 3496 3687 33
Size: 273 666 846 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 666 273 191
SfoI GGC'GCC
Cuts at: 2207 2455 2207
Size: 248 4527
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
SmaI CCC'GGG
Cuts at: 1797 1797
Size: 4775
SmlI C'TyrA_G
Cuts at: 589 1930 2845 3337 3599 589
Size: 1341 915 492 262 1765
Fragments arranged by size:
1765 1341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4775
SphI G_CATG'C
Cuts at: 1828 2328 1828
Size: 500 4275
SspI AAT'ATT
Cuts at: 741 741
Size: 4775
StuI AGG'CCT
Cuts at: 1363 1558 1363
Size: 195 4580
StyI C'CwwG_G
Cuts at: 107 129 429 1909 2263 107
Size: 22 300 1480 354 2619
Fragments arranged by size:
2619 1480 354 300 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1504 2358 2491 2950 3159 277
Size: 1227 854 133 459 209 1893
Fragments arranged by size:
1893 1227 854 459 209 133
TatI w'GTAC_w
Cuts at: 613 1148 4290 613
Size: 535 3142 1098
Fragments arranged by size:
3142 1098 535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 4292 4324 179
Size: 32 4081 32 630
Fragments arranged by size:
4081 630 32 32
Tth111I GACn'n_nGTC
Cuts at: 2572 2572
Size: 4775
XbaI T'CTAG_A
Cuts at: 1806 1806
Size: 4775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 2270
Size: 4775
XmaI C'CCGG_G
Cuts at: 1795 1795
Size: 4775
XmnI GAAnn'nnTTC
Cuts at: 1054 1138 1222 1642 1763 1054
Size: 84 84 420 121 4066
Fragments arranged by size:
4066 420 121 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PasI PflMI PfoI PshAI
PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI