Restriction map for
pF1KB7868
(Circular) (Six-base) MAPSORT of: pF1KB7868.seq Check: 6343 from: 1 to: 5015
pF1KB7868 5015 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1001 2053 1001
Size: 1052 3963
Acc65I G'GTAC_C
Cuts at: 2031 2031
Size: 5015
AclI AA'CG_TT
Cuts at: 2009 4855 2009
Size: 2846 2169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 2840 3272 4019 4287 717
Size: 2123 432 747 268 1445
Fragments arranged by size:
2123 1445 747 432 268
AflIII A'CryG_T
Cuts at: 204 1944 2367 3471 4448 204
Size: 1740 423 1104 977 771
Fragments arranged by size:
1740 1104 977 771 423
AgeI A'CCGG_T
Cuts at: 3403 3403
Size: 5015
AleI CACnn'nnGTG
Cuts at: 1486 1486
Size: 5015
AlwNI CAG_nnn'CTG
Cuts at: 193 261 790 841 1386 2320 3887 193
Size: 68 529 51 545 934 1567 1321
Fragments arranged by size:
1567 1321 934 545 529 68 51
ApaLI G'TGCA_C
Cuts at: 534 3785 534
Size: 3251 1764
ApoI r'AATT_y
Cuts at: 2019 4368 4624 2019
Size: 2349 256 2410
Fragments arranged by size:
2410 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 2035 2035
Size: 5015
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 193 226 193
Size: 33 4982
BamHI G'GATC_C
Cuts at: 2040 2040
Size: 5015
BanI G'GyrC_C
Cuts at: 415 624 1805 2031 2445 2693 2728 415
Size: 209 1181 226 414 248 35 2702
Fragments arranged by size:
2702 1181 414 248 226 209 35
BanII G_rGCy'C
Cuts at: 318 670 950 1208 2029 3059 318
Size: 352 280 258 821 1030 2274
Fragments arranged by size:
2274 1030 821 352 280 258
BbeI G_GCGC'C
Cuts at: 419 2449 2697 419
Size: 2030 248 2737
Fragments arranged by size:
2737 2030 248
BbsI GAAGACnn'nnnn_
Cuts at: 1383 1397 1711 1383
Size: 14 314 4687
Fragments arranged by size:
4687 314 14
BbvCI CC'TCA_GC
Cuts at: 457 564 1174 457
Size: 107 610 4298
Fragments arranged by size:
4298 610 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1900 1934 1900
Size: 34 4981
BciVI GTATCCnnnnn_n'
Cuts at: 2913 3674 5015 2913
Size: 761 1341 2913
Fragments arranged by size:
2913 1341 761
BclI T'GATC_A
Cuts at: 357 2535 357
Size: 2178 2837
BglII A'GATC_T
Cuts at: 1059 1086 1559 2530 4567 1059
Size: 27 473 971 2037 1507
Fragments arranged by size:
2037 1507 971 473 27
BlpI GC'TnA_GC
Cuts at: 2127 2127
Size: 5015
Bme1580I G_kGCm'C
Cuts at: 234 538 646 1483 2640 2733 3789 234
Size: 304 108 837 1157 93 1056 1460
Fragments arranged by size:
1460 1157 1056 837 304 108 93
BmgBI CAC'GTC
Cuts at: 1484 1945 1484
Size: 461 4554
BmrI ACTGGGnnnn_n'
Cuts at: 125 392 1005 2227 2308 2643 4787 125
Size: 267 613 1222 81 335 2144 353
Fragments arranged by size:
2144 1222 613 353 335 267 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1898 3382 1898
Size: 1484 3531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 904 1084 1122 1550 672
Size: 232 180 38 428 4137
Fragments arranged by size:
4137 428 232 180 38
Bpu10I CC'TnA_GC
Cuts at: 457 564 1174 457
Size: 107 610 4298
Fragments arranged by size:
4298 610 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2191 3070 3562 3860 1705
Size: 486 879 492 298 2860
Fragments arranged by size:
2860 879 492 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 948 1723 4719 948
Size: 775 2996 1244
Fragments arranged by size:
2996 1244 775
BsaAI yAC'GTr
Cuts at: 1005 1617 2368 1005
Size: 612 751 3652
Fragments arranged by size:
3652 751 612
BsaBI GATnn'nnATC
Cuts at: 6 2529 4566 6
Size: 2523 2037 455
Fragments arranged by size:
2523 2037 455
BsaHI Gr'CG_yC
Cuts at: 416 2446 2694 3396 4892 416
Size: 2030 248 702 1496 539
Fragments arranged by size:
2030 1496 702 539 248
BsaWI w'CCGG_w
Cuts at: 950 2204 2725 3403 3677 3824 4235 950
Size: 1254 521 678 274 147 411 1730
Fragments arranged by size:
1730 1254 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1485 1515 1994 2024 1485
Size: 30 479 30 4476
Fragments arranged by size:
4476 479 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 110 330 830 1081 1090 1093 1237 1334 2015
Size: 220 500 251 9 3 144 97 681
Cuts at: 2015 110
Size: 3110
Fragments arranged by size:
3110 681 500 251 220 144 97 9
3
BseYI C'CCAG_C
Cuts at: 619 715 834 943 1026 2440 3198 3775 4271
Size: 96 119 109 83 1414 758 577 496
Cuts at: 4271 619
Size: 1363
Fragments arranged by size:
1414 1363 758 577 496 119 109 96
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 5015
BsiEI CG_ry'CG
Cuts at: 104 1351 1642 2557 2603 3811 104
Size: 1247 291 915 46 1208 1308
Fragments arranged by size:
1308 1247 1208 915 291 46
BsiHKAI G_wGCw'C
Cuts at: 509 538 1700 2029 2807 2997 3789 509
Size: 29 1162 329 778 190 792 1735
Fragments arranged by size:
1735 1162 792 778 329 190 29
Bsp1286I G_dGCh'C
Cuts at: 234 318 509 538 646 670 950 1208 1483
Size: 84 191 29 108 24 280 258 275
Cuts at: 1483 1700 2029 2640 2733 2807 2997 3059 3789
Size: 217 329 611 93 74 190 62 730
Cuts at: 3789 234
Size: 1460
Fragments arranged by size:
1460 730 611 329 280 275 258 217
191 190 108 93 84 74 62 29
24
BspEI T'CCGG_A
Cuts at: 950 4235 950
Size: 3285 1730
BspHI T'CATG_A
Cuts at: 4191 4191
Size: 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1218 1279 2065 2279 2581 2962 1218
Size: 61 786 214 302 381 3271
Fragments arranged by size:
3271 786 381 302 214 61
BsrBI CCG'CTC
Cuts at: 50 1353 3307 3361 4845 5010 50
Size: 1303 1954 54 1484 165 55
Fragments arranged by size:
1954 1484 1303 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2927 2927
Size: 5015
BsrFI r'CCGG_y
Cuts at: 1610 1802 3013 3194 3403 1610
Size: 192 1211 181 209 3222
Fragments arranged by size:
3222 1211 209 192 181
BssHII G'CGCG_C
Cuts at: 673 673
Size: 5015
BssSI C'ACGA_G
Cuts at: 3286 3644 3286
Size: 358 4657
BstAPI GCAn_nnn'nTGC
Cuts at: 261 790 2392 261
Size: 529 1602 2884
Fragments arranged by size:
2884 1602 529
BstBI TT'CG_AA
Cuts at: 3376 3376
Size: 5015
BstEII G'GTnAC_C
Cuts at: 222 222
Size: 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 405 405
Size: 5015
BstYI r'GATC_y
Cuts at: 1 1059 1086 1382 1559 2040 2507 2530 2865
Size: 1058 27 296 177 481 467 23 335
Cuts at: 2865 3111 4123 4209 4221 4567 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1058 1012 481 467 449 346 335 296
246 177 86 27 23 12
BstZ17I GTA'TAC
Cuts at: 1002 1002
Size: 5015
Bsu36I CC'TnA_GG
Cuts at: 218 1163 218
Size: 945 4070
BtgI C'CryG_G
Cuts at: 107 662 1472 1850 107
Size: 555 810 378 3272
Fragments arranged by size:
3272 810 555 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 293 917 2965 3146 3309 293
Size: 624 2048 181 163 1999
Fragments arranged by size:
2048 1999 624 181 163
BtsI GCAGTG_nn'
Cuts at: 203 800 807 858 935 1472 1548 2258 203
Size: 597 7 51 77 537 76 710 2960
Fragments arranged by size:
2960 710 597 537 77 76 51 7
DraI TTT'AAA
Cuts at: 2014 2014
Size: 5015
DraIII CAC_nnn'GTG
Cuts at: 525 1974 525
Size: 1449 3566
DrdI GACnn_nn'nnGTC
Cuts at: 2721 3579 2721
Size: 858 4157
EaeI y'GGCC_r
Cuts at: 450 1431 1639 1922 2600 2774 3165 3192 4537
Size: 981 208 283 678 174 391 27 1345
Cuts at: 4537 450
Size: 928
Fragments arranged by size:
1345 981 928 678 391 283 208 174
27
EagI C'GGCC_G
Cuts at: 1639 2600 1639
Size: 961 4054
EarI CTCTTCn'nnn_
Cuts at: 1067 1326 3038 3248 1067
Size: 259 1712 210 2834
Fragments arranged by size:
2834 1712 259 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 158 3223 3533 3679 4825 158
Size: 3065 310 146 1146 348
Fragments arranged by size:
3065 1146 348 310 146
EcoICRI GAG'CTC
Cuts at: 2027 2027
Size: 5015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 717 904 1084 1122 1550 2840 3272 4019
Size: 45 187 180 38 428 1290 432 747
Cuts at: 4019 4287 672
Size: 268 1400
Fragments arranged by size:
1400 1290 747 432 428 268 187 180
45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1286 1286
Size: 5015
EcoO109I rG'GnC_Cy
Cuts at: 363 1159 1279 1524 2154 363
Size: 796 120 245 630 3224
Fragments arranged by size:
3224 796 630 245 120
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 5015
FspI TGC'GCA
Cuts at: 2796 2796
Size: 5015
HaeII r_GCGC'y
Cuts at: 419 1033 1803 2449 2697 3719 4698 419
Size: 614 770 646 248 1022 979 736
Fragments arranged by size:
1022 979 770 736 646 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 745 777 2857 2887 2889 2919 694
Size: 32 19 32 2080 30 2 30 2790
Fragments arranged by size:
2790 2080 32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 483 1097 1233 2054 483
Size: 614 136 821 3444
Fragments arranged by size:
3444 821 614 136
KasI G'GCGC_C
Cuts at: 415 2445 2693 415
Size: 2030 248 2737
Fragments arranged by size:
2737 2030 248
KpnI G_GTAC'C
Cuts at: 2035 2035
Size: 5015
MluI A'CGCG_T
Cuts at: 4448 4448
Size: 5015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3686 3870 3686
Size: 184 4831
MscI TGG'CCA
Cuts at: 1433 2776 1433
Size: 1343 3672
MslI CAynn'nnrTG
Cuts at: 1486 3004 3131 1486
Size: 1518 127 3370
Fragments arranged by size:
3370 1518 127
MspA1I CmG'CkG
Cuts at: 156 193 1904 2126 2440 2800 3813 4058 156
Size: 37 1711 222 314 360 1013 245 1113
Fragments arranged by size:
1711 1113 1013 360 314 245 222 37
NaeI GCC'GGC
Cuts at: 1804 3196 1804
Size: 1392 3623
NarI GG'CG_CC
Cuts at: 416 2446 2694 416
Size: 2030 248 2737
Fragments arranged by size:
2737 2030 248
NcoI C'CATG_G
Cuts at: 107 662 107
Size: 555 4460
NdeI CA'TA_TG
Cuts at: 1266 1266
Size: 5015
NgoMIV G'CCGG_C
Cuts at: 1802 3194 1802
Size: 1392 3623
NspI r_CATG'y
Cuts at: 208 1371 1605 1626 2068 2568 3475 208
Size: 1163 234 21 442 500 907 1748
Fragments arranged by size:
1748 1163 907 500 442 234 21
PasI CC'CwG_GG
Cuts at: 740 740
Size: 5015
PciI A'CATG_T
Cuts at: 204 3471 204
Size: 3267 1748
PflMI CCAn_nnn'nTGG
Cuts at: 1386 1974 1386
Size: 588 4427
PfoI T'CCnGG_A
Cuts at: 631 1884 631
Size: 1253 3762
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PmlI CAC'GTG
Cuts at: 1617 1617
Size: 5015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4983
PpuMI rG'GwC_Cy
Cuts at: 363 1279 1524 363
Size: 916 245 3854
Fragments arranged by size:
3854 916 245
PstI C_TGCA'G
Cuts at: 198 266 594 795 2062 2747 198
Size: 68 328 201 1267 685 2466
Fragments arranged by size:
2466 1267 685 328 201 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 193 2440 2800 193
Size: 2247 360 2408
Fragments arranged by size:
2408 2247 360
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 2029 2029
Size: 5015
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SanDI GG'GwC_CC
Cuts at: 1524 1524
Size: 5015
SapI GCTCTTCn'nnn_
Cuts at: 3038 3248 3038
Size: 210 4805
SbfI CC_TGCA'GG
Cuts at: 2062 2062
Size: 5015
ScaI AGT'ACT
Cuts at: 4532 4532
Size: 5015
SfcI C'TryA_G
Cuts at: 33 194 262 590 791 2058 2743 3736 3927
Size: 161 68 328 201 1267 685 993 191
Cuts at: 3927 33
Size: 1121
Fragments arranged by size:
1267 1121 993 685 328 201 191 161
68
SfoI GGC'GCC
Cuts at: 417 2447 2695 417
Size: 2030 248 2737
Fragments arranged by size:
2737 2030 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 2037 2037
Size: 5015
SmlI C'TyrA_G
Cuts at: 1720 2170 3085 3577 3839 1720
Size: 450 915 492 262 2896
Fragments arranged by size:
2896 915 492 450 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5015
SphI G_CATG'C
Cuts at: 1605 2068 2568 1605
Size: 463 500 4052
Fragments arranged by size:
4052 500 463
StuI AGG'CCT
Cuts at: 1585 1585
Size: 5015
StyI C'CwwG_G
Cuts at: 107 662 1361 2149 2503 107
Size: 555 699 788 354 2619
Fragments arranged by size:
2619 788 699 555 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1489 1974 2598 2731 3190 3399 1489
Size: 485 624 133 459 209 3105
Fragments arranged by size:
3105 624 485 459 209 133
TatI w'GTAC_w
Cuts at: 4530 4530
Size: 5015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1485 1517 1527 1559 1949 1981 4532 4564 1485
Size: 32 10 32 390 32 2551 32 1936
Fragments arranged by size:
2551 1936 390 32 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 478 2812 478
Size: 2334 2681
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 992 1146 2510 234
Size: 758 154 1364 2739
Fragments arranged by size:
2739 1364 758 154
XmaI C'CCGG_G
Cuts at: 2035 2035
Size: 5015
XmnI GAAnn'nnTTC
Cuts at: 1585 1585
Size: 5015
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI