Restriction map for
pF1KB7885
(Circular) (Six-base) MAPSORT of: pF1KB7885.seq Check: 9661 from: 1 to: 4856
pF1KB7885 4856 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1894 1894
Size: 4856
Acc65I G'GTAC_C
Cuts at: 1872 1872
Size: 4856
AclI AA'CG_TT
Cuts at: 4696 4696
Size: 4856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1244 1310 2681 3113 3860 4128 254
Size: 990 66 1371 432 747 268 982
Fragments arranged by size:
1371 990 982 747 432 268 66
AflIII A'CryG_T
Cuts at: 1256 2208 3312 4289 1256
Size: 952 1104 977 1823
Fragments arranged by size:
1823 1104 977 952
AgeI A'CCGG_T
Cuts at: 3244 3244
Size: 4856
AhdI GACnn_n'nnGTC
Cuts at: 1614 1656 1614
Size: 42 4814
AleI CACnn'nnGTG
Cuts at: 617 724 1292 617
Size: 107 568 4181
Fragments arranged by size:
4181 568 107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 514 546 514
Size: 32 4824
AlwNI CAG_nnn'CTG
Cuts at: 783 1200 1481 2161 3728 783
Size: 417 281 680 1567 1911
Fragments arranged by size:
1911 1567 680 417 281
ApaLI G'TGCA_C
Cuts at: 145 153 3626 145
Size: 8 3473 1375
Fragments arranged by size:
3473 1375 8
ApoI r'AATT_y
Cuts at: 380 813 1125 1384 1597 1860 4209 4465 380
Size: 433 312 259 213 263 2349 256 771
Fragments arranged by size:
2349 771 433 312 263 259 256 213
AseI AT'TA_AT
Cuts at: 20 20
Size: 4856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
AvaI C'yCGr_G
Cuts at: 1876 1876
Size: 4856
BamHI G'GATC_C
Cuts at: 348 1881 348
Size: 1533 3323
BanI G'GyrC_C
Cuts at: 1412 1872 2286 2534 2569 1412
Size: 460 414 248 35 3699
Fragments arranged by size:
3699 460 414 248 35
BanII G_rGCy'C
Cuts at: 1870 2900 1870
Size: 1030 3826
BbeI G_GCGC'C
Cuts at: 2290 2538 2290
Size: 248 4608
BbsI GAAGACnn'nnnn_
Cuts at: 178 550 1567 1618 178
Size: 372 1017 51 3416
Fragments arranged by size:
3416 1017 372 51
BciVI GTATCCnnnnn_n'
Cuts at: 2754 3515 4856 2754
Size: 761 1341 2754
Fragments arranged by size:
2754 1341 761
BclI T'GATC_A
Cuts at: 330 2376 330
Size: 2046 2810
BglII A'GATC_T
Cuts at: 1216 2371 4408 1216
Size: 1155 2037 1664
Fragments arranged by size:
2037 1664 1155
BlpI GC'TnA_GC
Cuts at: 1968 1968
Size: 4856
Bme1580I G_kGCm'C
Cuts at: 149 157 2481 2574 3630 149
Size: 8 2324 93 1056 1375
Fragments arranged by size:
2324 1375 1056 93 8
BmrI ACTGGGnnnn_n'
Cuts at: 1269 2068 2149 2484 4628 1269
Size: 799 81 335 2144 1497
Fragments arranged by size:
2144 1497 799 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1365 3223 1365
Size: 1858 2998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 904 1543 250
Size: 654 639 3563
Fragments arranged by size:
3563 654 639
Bpu10I CC'TnA_GC
Cuts at: 696 696
Size: 4856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1526 2032 2911 3403 3701 1030
Size: 496 506 879 492 298 2185
Fragments arranged by size:
2185 879 506 496 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4560 4560
Size: 4856
BsaAI yAC'GTr
Cuts at: 2209 2209
Size: 4856
BsaBI GATnn'nnATC
Cuts at: 6 2370 4407 6
Size: 2364 2037 455
Fragments arranged by size:
2364 2037 455
BsaHI Gr'CG_yC
Cuts at: 2287 2535 3237 4733 2287
Size: 248 702 1496 2410
Fragments arranged by size:
2410 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2045 2566 3244 3518 3665 4076 2045
Size: 521 678 274 147 411 2825
Fragments arranged by size:
2825 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 514 544 1229 1259 514
Size: 30 685 30 4111
Fragments arranged by size:
4111 685 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 253 1051 1054 211
Size: 42 798 3 4013
Fragments arranged by size:
4013 798 42 3
BseYI C'CCAG_C
Cuts at: 118 158 2281 3039 3616 4112 118
Size: 40 2123 758 577 496 862
Fragments arranged by size:
2123 862 758 577 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1316
Size: 4856
BsiEI CG_ry'CG
Cuts at: 104 2398 2444 3652 104
Size: 2294 46 1208 1308
Fragments arranged by size:
2294 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 157 1870 2648 2838 3630 149
Size: 8 1713 778 190 792 1375
Fragments arranged by size:
1713 1375 792 778 190 8
Bsp1286I G_dGCh'C
Cuts at: 149 157 1870 2481 2574 2648 2838 2900 3630
Size: 8 1713 611 93 74 190 62 730
Cuts at: 3630 149
Size: 1375
Fragments arranged by size:
1713 1375 730 611 190 93 74 62
8
BspEI T'CCGG_A
Cuts at: 4076 4076
Size: 4856
BspHI T'CATG_A
Cuts at: 735 4032 735
Size: 3297 1559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1906 2120 2422 2803 1906
Size: 214 302 381 3959
Fragments arranged by size:
3959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1014 3148 3202 4686 4851 50
Size: 964 2134 54 1484 165 55
Fragments arranged by size:
2134 1484 964 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1172 2768 1172
Size: 1596 3260
BsrFI r'CCGG_y
Cuts at: 2854 3035 3244 2854
Size: 181 209 4466
Fragments arranged by size:
4466 209 181
BsrGI T'GTAC_A
Cuts at: 1422 1422
Size: 4856
BssSI C'ACGA_G
Cuts at: 3127 3485 3127
Size: 358 4498
BstAPI GCAn_nnn'nTGC
Cuts at: 1365 2233 1365
Size: 868 3988
BstBI TT'CG_AA
Cuts at: 939 3217 939
Size: 2278 2578
BstEII G'GTnAC_C
Cuts at: 1083 1083
Size: 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1784 114
Size: 1670 3186
BstYI r'GATC_y
Cuts at: 1 348 1109 1216 1477 1802 1881 2348 2371
Size: 347 761 107 261 325 79 467 23
Cuts at: 2371 2706 2952 3964 4050 4062 4408 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 761 467 449 347 346 335 325
261 246 107 86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 1739 1739
Size: 4856
BtgI C'CryG_G
Cuts at: 107 107
Size: 4856
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2806 2987 3150 2806
Size: 181 163 4512
Fragments arranged by size:
4512 181 163
BtsI GCAGTG_nn'
Cuts at: 367 2099 367
Size: 1732 3124
DraI TTT'AAA
Cuts at: 1855 1855
Size: 4856
DrdI GACnn_nn'nnGTC
Cuts at: 2562 3420 2562
Size: 858 3998
EaeI y'GGCC_r
Cuts at: 506 2441 2615 3006 3033 4378 506
Size: 1935 174 391 27 1345 984
Fragments arranged by size:
1935 1345 984 391 174 27
EagI C'GGCC_G
Cuts at: 2441 2441
Size: 4856
EarI CTCTTCn'nnn_
Cuts at: 230 1220 2879 3089 230
Size: 990 1659 210 1997
Fragments arranged by size:
1997 1659 990 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3064 3374 3520 4666 3064
Size: 310 146 1146 3254
Fragments arranged by size:
3254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1868 1868
Size: 4856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 254 904 1244 1310 1543 2681 3113 3860
Size: 4 650 340 66 233 1138 432 747
Cuts at: 3860 4128 250
Size: 268 978
Fragments arranged by size:
1138 978 747 650 432 340 268 233
66 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1766 1766
Size: 4856
EcoO109I rG'GnC_Cy
Cuts at: 700 843 944 1995 700
Size: 143 101 1051 3561
Fragments arranged by size:
3561 1051 143 101
EcoRI G'AATT_C
Cuts at: 813 1860 813
Size: 1047 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4856
FspI TGC'GCA
Cuts at: 2637 2637
Size: 4856
HaeII r_GCGC'y
Cuts at: 2290 2538 3560 4539 2290
Size: 248 1022 979 2607
Fragments arranged by size:
2607 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 333 365 949 981 1462 1494 1600 1632 1642
Size: 32 584 32 481 32 106 32 10
Cuts at: 1642 1674 2698 2728 2730 2760 333
Size: 32 1024 30 2 30 2429
Fragments arranged by size:
2429 1024 584 481 106 32 32 32
32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 1284 1895 1284
Size: 611 4245
KasI G'GCGC_C
Cuts at: 2286 2534 2286
Size: 248 4608
KpnI G_GTAC'C
Cuts at: 1876 1876
Size: 4856
MfeI C'AATT_G
Cuts at: 221 221
Size: 4856
MluI A'CGCG_T
Cuts at: 4289 4289
Size: 4856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3527 3711 3527
Size: 184 4672
MscI TGG'CCA
Cuts at: 508 2617 508
Size: 2109 2747
MslI CAynn'nnrTG
Cuts at: 112 617 724 1292 2845 2972 112
Size: 505 107 568 1553 127 1996
Fragments arranged by size:
1996 1553 568 505 127 107
MspA1I CmG'CkG
Cuts at: 141 1967 2281 2641 3654 3899 141
Size: 1826 314 360 1013 245 1098
Fragments arranged by size:
1826 1098 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3037 3037
Size: 4856
NarI GG'CG_CC
Cuts at: 2287 2535 2287
Size: 248 4608
NcoI C'CATG_G
Cuts at: 107 107
Size: 4856
NgoMIV G'CCGG_C
Cuts at: 3035 3035
Size: 4856
NspI r_CATG'y
Cuts at: 1260 1909 2409 3316 1260
Size: 649 500 907 2800
Fragments arranged by size:
2800 907 649 500
PasI CC'CwG_GG
Cuts at: 1433 1433
Size: 4856
PciI A'CATG_T
Cuts at: 1256 3312 1256
Size: 2056 2800
PfoI T'CCnGG_A
Cuts at: 1112 1373 1112
Size: 261 4595
PmeI GTTT'AAAC
Cuts at: 1855 1855
Size: 4856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 514 546 3995 4027 514
Size: 32 3449 32 1343
Fragments arranged by size:
3449 1343 32 32
PpuMI rG'GwC_Cy
Cuts at: 843 944 843
Size: 101 4755
PshAI GACnn'nnGTC
Cuts at: 949 1694 949
Size: 745 4111
PsiI TTA'TAA
Cuts at: 310 310
Size: 4856
PstI C_TGCA'G
Cuts at: 483 1903 2588 483
Size: 1420 685 2751
Fragments arranged by size:
2751 1420 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4856
PvuII CAG'CTG
Cuts at: 2281 2641 2281
Size: 360 4496
RsrII CG'GwC_CG
Cuts at: 3051 3051
Size: 4856
SacI G_AGCT'C
Cuts at: 1870 1870
Size: 4856
SalI G'TCGA_C
Cuts at: 1893 1893
Size: 4856
SapI GCTCTTCn'nnn_
Cuts at: 1220 2879 3089 1220
Size: 1659 210 2987
Fragments arranged by size:
2987 1659 210
SbfI CC_TGCA'GG
Cuts at: 1903 1903
Size: 4856
ScaI AGT'ACT
Cuts at: 4373 4373
Size: 4856
SfcI C'TryA_G
Cuts at: 33 479 1899 2584 3577 3768 33
Size: 446 1420 685 993 191 1121
Fragments arranged by size:
1420 1121 993 685 446 191
SfoI GGC'GCC
Cuts at: 2288 2536 2288
Size: 248 4608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
SmaI CCC'GGG
Cuts at: 1878 1878
Size: 4856
SmlI C'TyrA_G
Cuts at: 1009 1541 2011 2926 3418 3680 1009
Size: 532 470 915 492 262 2185
Fragments arranged by size:
2185 915 532 492 470 262
SpeI A'CTAG_T
Cuts at: 67 931 67
Size: 864 3992
SphI G_CATG'C
Cuts at: 1909 2409 1909
Size: 500 4356
StyI C'CwwG_G
Cuts at: 107 509 839 1990 2344 107
Size: 402 330 1151 354 2619
Fragments arranged by size:
2619 1151 402 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 2439 2572 3031 3240 172
Size: 2267 133 459 209 1788
Fragments arranged by size:
2267 1788 459 209 133
TatI w'GTAC_w
Cuts at: 730 1422 4371 730
Size: 692 2949 1215
Fragments arranged by size:
2949 1215 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4373 4405 4373
Size: 32 4824
Tth111I GACn'n_nGTC
Cuts at: 2653 2653
Size: 4856
XbaI T'CTAG_A
Cuts at: 1887 1887
Size: 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 4856
XmaI C'CCGG_G
Cuts at: 1876 1876
Size: 4856
XmnI GAAnn'nnTTC
Cuts at: 175 1315 175
Size: 1140 3716
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI