Restriction map for
pF1KB7886
(Circular) (Six-base) MAPSORT of: pF1KB7886.seq Check: 3201 from: 1 to: 5066
pF1KB7886 5066 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2104 2104
Size: 5066
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5066
AclI AA'CG_TT
Cuts at: 4906 4906
Size: 5066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 926 1549 1633 1828 1886 2891 3323 4070
Size: 616 623 84 195 58 1005 432 747
Cuts at: 4070 4338 310
Size: 268 1038
Fragments arranged by size:
1038 1005 747 623 616 432 268 195
84 58
AflIII A'CryG_T
Cuts at: 130 661 2418 3522 4499 130
Size: 531 1757 1104 977 697
Fragments arranged by size:
1757 1104 977 697 531
AgeI A'CCGG_T
Cuts at: 3454 3454
Size: 5066
AlwNI CAG_nnn'CTG
Cuts at: 622 1572 1716 2371 3938 622
Size: 950 144 655 1567 1750
Fragments arranged by size:
1750 1567 950 655 144
ApaLI G'TGCA_C
Cuts at: 3836 3836
Size: 5066
ApoI r'AATT_y
Cuts at: 388 651 739 833 1351 1575 1822 2070 4419
Size: 263 88 94 518 224 247 248 2349
Cuts at: 4419 4675 388
Size: 256 779
Fragments arranged by size:
2349 779 518 263 256 248 247 224
94 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 5066
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
AvaI C'yCGr_G
Cuts at: 581 2086 581
Size: 1505 3561
BamHI G'GATC_C
Cuts at: 2091 2091
Size: 5066
BanI G'GyrC_C
Cuts at: 114 2082 2496 2744 2779 114
Size: 1968 414 248 35 2401
Fragments arranged by size:
2401 1968 414 248 35
BanII G_rGCy'C
Cuts at: 2026 2080 3110 2026
Size: 54 1030 3982
Fragments arranged by size:
3982 1030 54
BbeI G_GCGC'C
Cuts at: 2500 2748 2500
Size: 248 4818
BbsI GAAGACnn'nnnn_
Cuts at: 1668 1859 1668
Size: 191 4875
BciVI GTATCCnnnnn_n'
Cuts at: 2964 3725 5066 2964
Size: 761 1341 2964
Fragments arranged by size:
2964 1341 761
BclI T'GATC_A
Cuts at: 587 2586 587
Size: 1999 3067
BfrBI ATG'CAT
Cuts at: 880 880
Size: 5066
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5066
BglII A'GATC_T
Cuts at: 1386 1757 2581 4618 1386
Size: 371 824 2037 1834
Fragments arranged by size:
2037 1834 824 371
BlpI GC'TnA_GC
Cuts at: 2178 2178
Size: 5066
Bme1580I G_kGCm'C
Cuts at: 1949 2691 2784 3840 1949
Size: 742 93 1056 3175
Fragments arranged by size:
3175 1056 742 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 2045 2278 2359 2694 4838 203
Size: 1842 233 81 335 2144 431
Fragments arranged by size:
2144 1842 431 335 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 5066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1760 859
Size: 901 4165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 847 2242 3121 3613 3911 843
Size: 4 1395 879 492 298 1998
Fragments arranged by size:
1998 1395 879 492 298 4
BsaI GGTCTCn'nnnn_
Cuts at: 4770 4770
Size: 5066
BsaAI yAC'GTr
Cuts at: 2419 2419
Size: 5066
BsaBI GATnn'nnATC
Cuts at: 6 638 1136 1153 1969 2580 4617 6
Size: 632 498 17 816 611 2037 455
Fragments arranged by size:
2037 816 632 611 498 455 17
BsaHI Gr'CG_yC
Cuts at: 2497 2745 3447 4943 2497
Size: 248 702 1496 2620
Fragments arranged by size:
2620 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2255 2776 3454 3728 3875 4286 2255
Size: 521 678 274 147 411 3035
Fragments arranged by size:
3035 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1736 1766 1736
Size: 30 5036
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1757 1757
Size: 5066
BseYI C'CCAG_C
Cuts at: 177 2491 3249 3826 4322 177
Size: 2314 758 577 496 921
Fragments arranged by size:
2314 921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 1746 1774 1390
Size: 356 28 4682
Fragments arranged by size:
4682 356 28
BsiEI CG_ry'CG
Cuts at: 104 152 2608 2654 3862 104
Size: 48 2456 46 1208 1308
Fragments arranged by size:
2456 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 2080 2858 3048 3840 2080
Size: 778 190 792 3306
Fragments arranged by size:
3306 792 778 190
BsmI GAATG_Cn'
Cuts at: 882 1444 882
Size: 562 4504
Bsp1286I G_dGCh'C
Cuts at: 1949 2026 2080 2691 2784 2858 3048 3110 3840
Size: 77 54 611 93 74 190 62 730
Cuts at: 3840 1949
Size: 3175
Fragments arranged by size:
3175 730 611 190 93 77 74 62
54
BspEI T'CCGG_A
Cuts at: 4286 4286
Size: 5066
BspHI T'CATG_A
Cuts at: 4242 4242
Size: 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2116 2330 2632 3013 2116
Size: 214 302 381 4169
Fragments arranged by size:
4169 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1535 1584 3358 3412 4896 5061 50
Size: 1485 49 1774 54 1484 165 55
Fragments arranged by size:
1774 1485 1484 165 55 54 49
BsrDI GCAATG_nn'
Cuts at: 2978 2978
Size: 5066
BsrFI r'CCGG_y
Cuts at: 3064 3245 3454 3064
Size: 181 209 4676
Fragments arranged by size:
4676 209 181
BsrGI T'GTAC_A
Cuts at: 1197 2039 1197
Size: 842 4224
BssSI C'ACGA_G
Cuts at: 3337 3695 3337
Size: 358 4708
BstAPI GCAn_nnn'nTGC
Cuts at: 2443 2443
Size: 5066
BstBI TT'CG_AA
Cuts at: 753 3427 753
Size: 2674 2392
BstYI r'GATC_y
Cuts at: 1 345 1131 1386 1757 2091 2558 2581 2916
Size: 344 786 255 371 334 467 23 335
Cuts at: 2916 3162 4174 4260 4272 4618 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 786 467 449 371 346 344 335
334 255 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 709 944 1464 107
Size: 602 235 520 3709
Fragments arranged by size:
3709 602 520 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 617 1466 3016 3197 3360 617
Size: 849 1550 181 163 2323
Fragments arranged by size:
2323 1550 849 181 163
BtsI GCAGTG_nn'
Cuts at: 2309 2309
Size: 5066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 713 748 2000 2018 2035 2053 713
Size: 35 1252 18 17 18 3726
Fragments arranged by size:
3726 1252 35 18 18 17
DraI TTT'AAA
Cuts at: 2065 2065
Size: 5066
DrdI GACnn_nn'nnGTC
Cuts at: 2772 3630 2772
Size: 858 4208
EaeI y'GGCC_r
Cuts at: 149 2651 2825 3216 3243 4588 149
Size: 2502 174 391 27 1345 627
Fragments arranged by size:
2502 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 149 2651 149
Size: 2502 2564
EarI CTCTTCn'nnn_
Cuts at: 482 1804 1862 3089 3299 482
Size: 1322 58 1227 210 2249
Fragments arranged by size:
2249 1322 1227 210 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3274 3584 3730 4876 3274
Size: 310 146 1146 3464
Fragments arranged by size:
3464 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2078 2078
Size: 5066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 859 926 1549 1633 1760 1828 1886 2891
Size: 549 67 623 84 127 68 58 1005
Cuts at: 2891 3323 4070 4338 310
Size: 432 747 268 1038
Fragments arranged by size:
1038 1005 747 623 549 432 268 127
84 68 67 58
EcoO109I rG'GnC_Cy
Cuts at: 1281 2205 1281
Size: 924 4142
EcoRI G'AATT_C
Cuts at: 388 2070 388
Size: 1682 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 473 3085 473
Size: 2612 2454
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5066
HaeII r_GCGC'y
Cuts at: 1047 2500 2748 3770 4749 1047
Size: 1453 248 1022 979 1364
Fragments arranged by size:
1453 1364 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 533 565 1014 1046 1689 1721 2908 2938 2940
Size: 32 449 32 643 32 1187 30 2
Cuts at: 2940 2970 533
Size: 30 2629
Fragments arranged by size:
2629 1187 643 449 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1398 1638 2105 1398
Size: 240 467 4359
Fragments arranged by size:
4359 467 240
HindIII A'AGCT_T
Cuts at: 1332 1332
Size: 5066
HpaI GTT'AAC
Cuts at: 1398 1638 1398
Size: 240 4826
KasI G'GCGC_C
Cuts at: 2496 2744 2496
Size: 248 4818
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5066
MluI A'CGCG_T
Cuts at: 130 4499 130
Size: 4369 697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 841 848 3737 3921 841
Size: 7 2889 184 1986
Fragments arranged by size:
2889 1986 184 7
MscI TGG'CCA
Cuts at: 2827 2827
Size: 5066
MslI CAynn'nnrTG
Cuts at: 774 1057 3055 3182 774
Size: 283 1998 127 2658
Fragments arranged by size:
2658 1998 283 127
MspA1I CmG'CkG
Cuts at: 339 1738 1773 2177 2491 2851 3864 4109 339
Size: 1399 35 404 314 360 1013 245 1296
Fragments arranged by size:
1399 1296 1013 404 360 314 245 35
NaeI GCC'GGC
Cuts at: 3247 3247
Size: 5066
NarI GG'CG_CC
Cuts at: 2497 2745 2497
Size: 248 4818
NcoI C'CATG_G
Cuts at: 107 1464 107
Size: 1357 3709
NgoMIV G'CCGG_C
Cuts at: 3245 3245
Size: 5066
NotI GC'GGCC_GC
Cuts at: 149 149
Size: 5066
NruI TCG'CGA
Cuts at: 1142 1142
Size: 5066
NsiI A_TGCA'T
Cuts at: 882 882
Size: 5066
NspI r_CATG'y
Cuts at: 665 2046 2119 2619 3526 665
Size: 1381 73 500 907 2205
Fragments arranged by size:
2205 1381 907 500 73
PciI A'CATG_T
Cuts at: 661 3522 661
Size: 2861 2205
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 5034
PpuMI rG'GwC_Cy
Cuts at: 1281 1281
Size: 5066
PshAI GACnn'nnGTC
Cuts at: 280 662 280
Size: 382 4684
PstI C_TGCA'G
Cuts at: 168 2113 2798 168
Size: 1945 685 2436
Fragments arranged by size:
2436 1945 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5066
PvuII CAG'CTG
Cuts at: 339 2491 2851 339
Size: 2152 360 2554
Fragments arranged by size:
2554 2152 360
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5066
SacI G_AGCT'C
Cuts at: 2080 2080
Size: 5066
SalI G'TCGA_C
Cuts at: 2103 2103
Size: 5066
SapI GCTCTTCn'nnn_
Cuts at: 1862 3089 3299 1862
Size: 1227 210 3629
Fragments arranged by size:
3629 1227 210
SbfI CC_TGCA'GG
Cuts at: 2113 2113
Size: 5066
ScaI AGT'ACT
Cuts at: 4583 4583
Size: 5066
SfcI C'TryA_G
Cuts at: 33 164 1034 1221 1873 2109 2794 3787 3978
Size: 131 870 187 652 236 685 993 191
Cuts at: 3978 33
Size: 1121
Fragments arranged by size:
1121 993 870 685 652 236 191 187
131
SfoI GGC'GCC
Cuts at: 2498 2746 2498
Size: 248 4818
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
SmaI CCC'GGG
Cuts at: 2088 2088
Size: 5066
SmlI C'TyrA_G
Cuts at: 822 862 2221 3136 3628 3890 822
Size: 40 1359 915 492 262 1998
Fragments arranged by size:
1998 1359 915 492 262 40
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5066
SphI G_CATG'C
Cuts at: 2119 2619 2119
Size: 500 4566
StyI C'CwwG_G
Cuts at: 107 457 1464 2200 2554 107
Size: 350 1007 736 354 2619
Fragments arranged by size:
2619 1007 736 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1195 2649 2782 3241 3450 1195
Size: 1454 133 459 209 2811
Fragments arranged by size:
2811 1454 459 209 133
TatI w'GTAC_w
Cuts at: 293 407 1197 2039 4581 293
Size: 114 790 842 2542 778
Fragments arranged by size:
2542 842 790 778 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1736 1768 4583 4615 1736
Size: 32 2815 32 2187
Fragments arranged by size:
2815 2187 32 32
Tth111I GACn'n_nGTC
Cuts at: 545 2863 545
Size: 2318 2748
XbaI T'CTAG_A
Cuts at: 2097 2097
Size: 5066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 707 2561 707
Size: 1854 3212
XmaI C'CCGG_G
Cuts at: 2086 2086
Size: 5066
XmnI GAAnn'nnTTC
Cuts at: 740 2007 740
Size: 1267 3799
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI
PciI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI MfeI NdeI
NheI PacI PasI PflMI PfoI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI