Restriction map for
pF1KB7888
(Circular) (Six-base) MAPSORT of: pF1KB7888.seq Check: 4624 from: 1 to: 5066
pF1KB7888 5066 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 2055 2104 2055
Size: 49 5017
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5066
AclI AA'CG_TT
Cuts at: 4906 4906
Size: 5066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 839 1743 2891 3323 4070 4338 237
Size: 602 904 1148 432 747 268 965
Fragments arranged by size:
1148 965 904 747 602 432 268
AflIII A'CryG_T
Cuts at: 2418 3522 4499 2418
Size: 1104 977 2985
Fragments arranged by size:
2985 1104 977
AgeI A'CCGG_T
Cuts at: 3454 3454
Size: 5066
AlwNI CAG_nnn'CTG
Cuts at: 225 2371 3938 225
Size: 2146 1567 1353
Fragments arranged by size:
2146 1567 1353
ApaLI G'TGCA_C
Cuts at: 1298 3836 1298
Size: 2538 2528
ApoI r'AATT_y
Cuts at: 367 475 713 845 1025 1174 2007 2070 4419
Size: 108 238 132 180 149 833 63 2349
Cuts at: 4419 4675 367
Size: 256 758
Fragments arranged by size:
2349 833 758 256 238 180 149 132
108 63
AseI AT'TA_AT
Cuts at: 20 1361 20
Size: 1341 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
AvaI C'yCGr_G
Cuts at: 1043 2086 1043
Size: 1043 4023
BamHI G'GATC_C
Cuts at: 1476 2091 1476
Size: 615 4451
BanI G'GyrC_C
Cuts at: 184 322 2082 2496 2744 2779 184
Size: 138 1760 414 248 35 2471
Fragments arranged by size:
2471 1760 414 248 138 35
BanII G_rGCy'C
Cuts at: 271 2080 3110 271
Size: 1809 1030 2227
Fragments arranged by size:
2227 1809 1030
BbeI G_GCGC'C
Cuts at: 326 2500 2748 326
Size: 2174 248 2644
Fragments arranged by size:
2644 2174 248
BciVI GTATCCnnnnn_n'
Cuts at: 2964 3725 5066 2964
Size: 761 1341 2964
Fragments arranged by size:
2964 1341 761
BclI T'GATC_A
Cuts at: 2586 2586
Size: 5066
BfrBI ATG'CAT
Cuts at: 1804 1804
Size: 5066
BglI GCCn_nnn'nGGC
Cuts at: 182 182
Size: 5066
BglII A'GATC_T
Cuts at: 723 792 2581 4618 723
Size: 69 1789 2037 1171
Fragments arranged by size:
2037 1789 1171 69
BlpI GC'TnA_GC
Cuts at: 1458 2178 1458
Size: 720 4346
Bme1580I G_kGCm'C
Cuts at: 423 1302 2691 2784 3840 423
Size: 879 1389 93 1056 1649
Fragments arranged by size:
1649 1389 1056 879 93
BmrI ACTGGGnnnn_n'
Cuts at: 2278 2359 2694 4838 2278
Size: 81 335 2144 2506
Fragments arranged by size:
2506 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 5066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 887 590
Size: 297 4769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1287 1322 2242 3121 3613 3911 1287
Size: 35 920 879 492 298 2442
Fragments arranged by size:
2442 920 879 492 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 4770 4770
Size: 5066
BsaAI yAC'GTr
Cuts at: 2419 2419
Size: 5066
BsaBI GATnn'nnATC
Cuts at: 6 136 797 2580 4617 6
Size: 130 661 1783 2037 455
Fragments arranged by size:
2037 1783 661 455 130
BsaHI Gr'CG_yC
Cuts at: 323 2497 2745 3447 4943 323
Size: 2174 248 702 1496 446
Fragments arranged by size:
2174 1496 702 446 248
BsaWI w'CCGG_w
Cuts at: 1197 2255 2776 3454 3728 3875 4286 1197
Size: 1058 521 678 274 147 411 1977
Fragments arranged by size:
1977 1058 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 292 295 289
Size: 3 3 5060
Fragments arranged by size:
5060 3 3
BseYI C'CCAG_C
Cuts at: 239 2491 3249 3826 4322 239
Size: 2252 758 577 496 983
Fragments arranged by size:
2252 983 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 2024 761
Size: 1263 3803
BsiEI CG_ry'CG
Cuts at: 104 2608 2654 3862 104
Size: 2504 46 1208 1308
Fragments arranged by size:
2504 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1302 2080 2858 3048 3840 1302
Size: 778 778 190 792 2528
Fragments arranged by size:
2528 792 778 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 184 184
Size: 5066
Bsp1286I G_dGCh'C
Cuts at: 271 423 1302 2080 2691 2784 2858 3048 3110
Size: 152 879 778 611 93 74 190 62
Cuts at: 3110 3840 271
Size: 730 1497
Fragments arranged by size:
1497 879 778 730 611 190 152 93
74 62
BspEI T'CCGG_A
Cuts at: 1197 4286 1197
Size: 3089 1977
BspHI T'CATG_A
Cuts at: 1277 4242 1277
Size: 2965 2101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 150 206 2116 2330 2632 3013 150
Size: 56 1910 214 302 381 2203
Fragments arranged by size:
2203 1910 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 3358 3412 4896 5061 50
Size: 3308 54 1484 165 55
Fragments arranged by size:
3308 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1198 2978 1198
Size: 1780 3286
BsrFI r'CCGG_y
Cuts at: 3064 3245 3454 3064
Size: 181 209 4676
Fragments arranged by size:
4676 209 181
BsrGI T'GTAC_A
Cuts at: 1620 1620
Size: 5066
BssHII G'CGCG_C
Cuts at: 120 120
Size: 5066
BssSI C'ACGA_G
Cuts at: 3337 3695 3337
Size: 358 4708
BstAPI GCAn_nnn'nTGC
Cuts at: 1230 2443 1230
Size: 1213 3853
BstBI TT'CG_AA
Cuts at: 696 3427 696
Size: 2731 2335
BstEII G'GTnAC_C
Cuts at: 913 913
Size: 5066
BstXI CCAn_nnnn'nTGG
Cuts at: 1737 1818 1737
Size: 81 4985
BstYI r'GATC_y
Cuts at: 1 723 745 792 1476 2091 2558 2581 2916
Size: 722 22 47 684 615 467 23 335
Cuts at: 2916 3162 4174 4260 4272 4618 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 722 684 615 467 449 346 335
246 86 47 23 22 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3016 3197 3360 3016
Size: 181 163 4722
Fragments arranged by size:
4722 181 163
BtsI GCAGTG_nn'
Cuts at: 131 2309 131
Size: 2178 2888
DraI TTT'AAA
Cuts at: 2065 2065
Size: 5066
DraIII CAC_nnn'GTG
Cuts at: 1083 1083
Size: 5066
DrdI GACnn_nn'nnGTC
Cuts at: 1331 2772 3630 1331
Size: 1441 858 2767
Fragments arranged by size:
2767 1441 858
EaeI y'GGCC_r
Cuts at: 316 1308 2651 2825 3216 3243 4588 316
Size: 992 1343 174 391 27 1345 794
Fragments arranged by size:
1345 1343 992 794 391 174 27
EagI C'GGCC_G
Cuts at: 2651 2651
Size: 5066
EarI CTCTTCn'nnn_
Cuts at: 940 1629 3089 3299 940
Size: 689 1460 210 2707
Fragments arranged by size:
2707 1460 689 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3274 3584 3730 4876 3274
Size: 310 146 1146 3464
Fragments arranged by size:
3464 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2078 2078
Size: 5066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 590 839 887 1743 2891 3323 4070 4338
Size: 353 249 48 856 1148 432 747 268
Cuts at: 4338 237
Size: 965
Fragments arranged by size:
1148 965 856 747 432 353 268 249
48
EcoO109I rG'GnC_Cy
Cuts at: 1333 2205 1333
Size: 872 4194
EcoRI G'AATT_C
Cuts at: 2070 2070
Size: 5066
EcoRV GAT'ATC
Cuts at: 548 548
Size: 5066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1319 3085 1319
Size: 1766 3300
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5066
HaeII r_GCGC'y
Cuts at: 326 2500 2748 3770 4749 326
Size: 2174 248 1022 979 643
Fragments arranged by size:
2174 1022 979 643 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 481 506 513 1089 1121 1150 1182 1272
Size: 7 25 7 576 32 29 32 90
Cuts at: 1272 1304 1476 1508 2908 2938 2940 2970 474
Size: 32 172 32 1400 30 2 30 2570
Fragments arranged by size:
2570 1400 576 172 90 32 32 32
32 30 30 29 25 7 7 2
HincII GTy'rAC
Cuts at: 930 2105 930
Size: 1175 3891
HindIII A'AGCT_T
Cuts at: 1340 1340
Size: 5066
HpaI GTT'AAC
Cuts at: 930 930
Size: 5066
KasI G'GCGC_C
Cuts at: 322 2496 2744 322
Size: 2174 248 2644
Fragments arranged by size:
2644 2174 248
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5066
MfeI C'AATT_G
Cuts at: 541 1588 541
Size: 1047 4019
MluI A'CGCG_T
Cuts at: 4499 4499
Size: 5066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 684 3737 3921 684
Size: 3053 184 1829
Fragments arranged by size:
3053 1829 184
MscI TGG'CCA
Cuts at: 1310 2827 1310
Size: 1517 3549
MslI CAynn'nnrTG
Cuts at: 1084 1211 1279 1628 1816 1961 3055 3182 1084
Size: 127 68 349 188 145 1094 127 2968
Fragments arranged by size:
2968 1094 349 188 145 127 127 68
MspA1I CmG'CkG
Cuts at: 207 243 2177 2491 2851 3864 4109 207
Size: 36 1934 314 360 1013 245 1164
Fragments arranged by size:
1934 1164 1013 360 314 245 36
NaeI GCC'GGC
Cuts at: 3247 3247
Size: 5066
NarI GG'CG_CC
Cuts at: 323 2497 2745 323
Size: 2174 248 2644
Fragments arranged by size:
2644 2174 248
NgoMIV G'CCGG_C
Cuts at: 3245 3245
Size: 5066
NsiI A_TGCA'T
Cuts at: 1806 1806
Size: 5066
NspI r_CATG'y
Cuts at: 1714 2119 2619 3526 1714
Size: 405 500 907 3254
Fragments arranged by size:
3254 907 500 405
PciI A'CATG_T
Cuts at: 3522 3522
Size: 5066
PflMI CCAn_nnn'nTGG
Cuts at: 171 171
Size: 5066
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 5034
PpuMI rG'GwC_Cy
Cuts at: 1333 1333
Size: 5066
PsiI TTA'TAA
Cuts at: 2018 2018
Size: 5066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1700 1732 1700
Size: 32 5034
PstI C_TGCA'G
Cuts at: 2113 2798 2113
Size: 685 4381
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5066
PvuII CAG'CTG
Cuts at: 2491 2851 2491
Size: 360 4706
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5066
SacI G_AGCT'C
Cuts at: 2080 2080
Size: 5066
SalI G'TCGA_C
Cuts at: 2103 2103
Size: 5066
SapI GCTCTTCn'nnn_
Cuts at: 3089 3299 3089
Size: 210 4856
SbfI CC_TGCA'GG
Cuts at: 2113 2113
Size: 5066
ScaI AGT'ACT
Cuts at: 1751 4583 1751
Size: 2832 2234
SexAI A'CCwGG_T
Cuts at: 963 1703 963
Size: 740 4326
SfcI C'TryA_G
Cuts at: 33 732 1669 1784 2109 2794 3787 3978 33
Size: 699 937 115 325 685 993 191 1121
Fragments arranged by size:
1121 993 937 699 685 325 191 115
SfoI GGC'GCC
Cuts at: 324 2498 2746 324
Size: 2174 248 2644
Fragments arranged by size:
2644 2174 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
SmaI CCC'GGG
Cuts at: 2088 2088
Size: 5066
SmlI C'TyrA_G
Cuts at: 1266 1337 2221 3136 3628 3890 1266
Size: 71 884 915 492 262 2442
Fragments arranged by size:
2442 915 884 492 262 71
SpeI A'CTAG_T
Cuts at: 67 2058 67
Size: 1991 3075
SphI G_CATG'C
Cuts at: 2119 2619 2119
Size: 500 4566
SspI AAT'ATT
Cuts at: 1596 1596
Size: 5066
StyI C'CwwG_G
Cuts at: 388 1730 2200 2554 388
Size: 1342 470 354 2900
Fragments arranged by size:
2900 1342 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 2649 2782 3241 3450 310
Size: 2339 133 459 209 1926
Fragments arranged by size:
2339 1926 459 209 133
TatI w'GTAC_w
Cuts at: 1620 1749 4581 1620
Size: 129 2832 2105
Fragments arranged by size:
2832 2105 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4583 4615 4583
Size: 32 5034
Tth111I GACn'n_nGTC
Cuts at: 2863 2863
Size: 5066
XbaI T'CTAG_A
Cuts at: 2097 2097
Size: 5066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2561 2561
Size: 5066
XmaI C'CCGG_G
Cuts at: 2086 2086
Size: 5066
XmnI GAAnn'nnTTC
Cuts at: 1052 1385 1052
Size: 333 4733
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI FseI FspAI NcoI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI