Restriction map for
pF1KB7893
(Circular) (Six-base) MAPSORT of: pF1KB7893.seq Check: 3831 from: 1 to: 4877
pF1KB7893 4877 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1915 1915
Size: 4877
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4877
AclI AA'CG_TT
Cuts at: 4717 4717
Size: 4877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 372 2702 3134 3881 4149 307
Size: 65 2330 432 747 268 1035
Fragments arranged by size:
2330 1035 747 432 268 65
AflIII A'CryG_T
Cuts at: 2229 3333 4310 2229
Size: 1104 977 2796
Fragments arranged by size:
2796 1104 977
AgeI A'CCGG_T
Cuts at: 3265 3265
Size: 4877
AlwNI CAG_nnn'CTG
Cuts at: 2182 3749 2182
Size: 1567 3310
ApaI G_GGCC'C
Cuts at: 181 181
Size: 4877
ApaLI G'TGCA_C
Cuts at: 1496 3647 1496
Size: 2151 2726
ApoI r'AATT_y
Cuts at: 1243 1608 1881 4230 4486 1243
Size: 365 273 2349 256 1634
Fragments arranged by size:
2349 1634 365 273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 541 569 748 1196 1649 1897 541
Size: 28 179 448 453 248 3521
Fragments arranged by size:
3521 453 448 248 179 28
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4877
BanI G'GyrC_C
Cuts at: 202 721 1627 1893 2307 2555 2590 202
Size: 519 906 266 414 248 35 2489
Fragments arranged by size:
2489 906 519 414 266 248 35
BanII G_rGCy'C
Cuts at: 148 172 181 434 542 755 809 1891 2921
Size: 24 9 253 108 213 54 1082 1030
Cuts at: 2921 148
Size: 2104
Fragments arranged by size:
2104 1082 1030 253 213 108 54 24
9
BbeI G_GCGC'C
Cuts at: 725 2311 2559 725
Size: 1586 248 3043
Fragments arranged by size:
3043 1586 248
BbvCI CC'TCA_GC
Cuts at: 260 260
Size: 4877
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3536 4877 2775
Size: 761 1341 2775
Fragments arranged by size:
2775 1341 761
BclI T'GATC_A
Cuts at: 1142 2397 1142
Size: 1255 3622
BfrBI ATG'CAT
Cuts at: 1367 1367
Size: 4877
BglI GCCn_nnn'nGGC
Cuts at: 176 185 176
Size: 9 4868
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 1989 1989
Size: 4877
Bme1580I G_kGCm'C
Cuts at: 181 245 1500 2502 2595 3651 181
Size: 64 1255 1002 93 1056 1407
Fragments arranged by size:
1407 1255 1056 1002 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 176 1864 2089 2170 2505 4649 176
Size: 1688 225 81 335 2144 404
Fragments arranged by size:
2144 1688 404 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1683 3244 1683
Size: 1561 3316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 214 418 736 1057 1090 1123 1327 1653
Size: 103 204 318 321 33 33 204 326
Cuts at: 1653 111
Size: 3335
Fragments arranged by size:
3335 326 321 318 204 204 103 33
33
Bpu10I CC'TnA_GC
Cuts at: 260 1402 260
Size: 1142 3735
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 931 2053 2932 3424 3722 688
Size: 243 1122 879 492 298 1843
Fragments arranged by size:
1843 1122 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 541 728 1588 4581 541
Size: 187 860 2993 837
Fragments arranged by size:
2993 860 837 187
BsaAI yAC'GTr
Cuts at: 2230 2230
Size: 4877
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 722 2308 2556 3258 4754 722
Size: 1586 248 702 1496 845
Fragments arranged by size:
1586 1496 845 702 248
BsaWI w'CCGG_w
Cuts at: 1500 1550 2066 2587 3265 3539 3686 4097 1500
Size: 50 516 521 678 274 147 411 2280
Fragments arranged by size:
2280 678 521 516 411 274 147 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 607 637 1630 1660 361
Size: 30 216 30 993 30 3578
Fragments arranged by size:
3578 993 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 628 904 1656 1707 628
Size: 276 752 51 3798
Fragments arranged by size:
3798 752 276 51
BseYI C'CCAG_C
Cuts at: 438 1772 2302 3060 3637 4133 438
Size: 1334 530 758 577 496 1182
Fragments arranged by size:
1334 1182 758 577 530 496
BsiEI CG_ry'CG
Cuts at: 104 2419 2465 3673 104
Size: 2315 46 1208 1308
Fragments arranged by size:
2315 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 809 863 1500 1891 2669 2859 3651 809
Size: 54 637 391 778 190 792 2035
Fragments arranged by size:
2035 792 778 637 391 190 54
BsiWI C'GTAC_G
Cuts at: 422 422
Size: 4877
BsmI GAATG_Cn'
Cuts at: 1673 1673
Size: 4877
BsmBI CGTCTCn'nnnn_
Cuts at: 904 1577 904
Size: 673 4204
Bsp1286I G_dGCh'C
Cuts at: 148 172 181 245 434 542 755 809 863
Size: 24 9 64 189 108 213 54 54
Cuts at: 863 1500 1891 2502 2595 2669 2859 2921 3651
Size: 637 391 611 93 74 190 62 730
Cuts at: 3651 148
Size: 1374
Fragments arranged by size:
1374 730 637 611 391 213 190 189
108 93 74 64 62 54 54 24
9
BspEI T'CCGG_A
Cuts at: 1550 4097 1550
Size: 2547 2330
BspHI T'CATG_A
Cuts at: 4053 4053
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1361 1703 1927 2141 2443 2824 838
Size: 523 342 224 214 302 381 2891
Fragments arranged by size:
2891 523 381 342 302 224 214
BsrBI CCG'CTC
Cuts at: 50 3169 3223 4707 4872 50
Size: 3119 54 1484 165 55
Fragments arranged by size:
3119 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 330 2789 330
Size: 2459 2418
BsrFI r'CCGG_y
Cuts at: 1820 2875 3056 3265 1820
Size: 1055 181 209 3432
Fragments arranged by size:
3432 1055 209 181
BssSI C'ACGA_G
Cuts at: 3148 3506 3148
Size: 358 4519
BstAPI GCAn_nnn'nTGC
Cuts at: 1806 2254 1806
Size: 448 4429
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstEII G'GTnAC_C
Cuts at: 1444 1444
Size: 4877
BstYI r'GATC_y
Cuts at: 1 1902 2369 2392 2727 2973 3985 4071 4083
Size: 1901 467 23 335 246 1012 86 12
Cuts at: 4083 4429 1
Size: 346 449
Fragments arranged by size:
1901 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 521 107
Size: 414 4463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1435 2827 3008 3171 1435
Size: 1392 181 163 3141
Fragments arranged by size:
3141 1392 181 163
BtsI GCAGTG_nn'
Cuts at: 922 2120 922
Size: 1198 3679
DraI TTT'AAA
Cuts at: 1876 1876
Size: 4877
DrdI GACnn_nn'nnGTC
Cuts at: 918 1699 1809 2583 3441 918
Size: 781 110 774 858 2354
Fragments arranged by size:
2354 858 781 774 110
EaeI y'GGCC_r
Cuts at: 1578 1822 2462 2636 3027 3054 4399 1578
Size: 244 640 174 391 27 1345 2056
Fragments arranged by size:
2056 1345 640 391 244 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 814 1507 2900 3110 814
Size: 693 1393 210 2581
Fragments arranged by size:
2581 1393 693 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 570 1411 3085 3395 3541 4687 570
Size: 841 1674 310 146 1146 760
Fragments arranged by size:
1674 1146 841 760 310 146
EcoICRI GAG'CTC
Cuts at: 807 1889 807
Size: 1082 3795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 214 307 372 418 736 1057 1090 1123
Size: 103 93 65 46 318 321 33 33
Cuts at: 1123 1327 1653 2702 3134 3881 4149 111
Size: 204 326 1049 432 747 268 839
Fragments arranged by size:
1049 839 747 432 326 321 318 268
204 103 93 65 46 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1306 1306
Size: 4877
EcoO109I rG'GnC_Cy
Cuts at: 178 1053 1065 1317 2016 178
Size: 875 12 252 699 3039
Fragments arranged by size:
3039 875 699 252 12
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 490 2658 490
Size: 2168 2709
HaeII r_GCGC'y
Cuts at: 284 311 725 2311 2559 3581 4560 284
Size: 27 414 1586 248 1022 979 601
Fragments arranged by size:
1586 1022 979 601 414 248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 329 361 393 2719 2749 2751 2781 329
Size: 32 32 2326 30 2 30 2425
Fragments arranged by size:
2425 2326 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1836 1916 1836
Size: 80 4797
KasI G'GCGC_C
Cuts at: 721 2307 2555 721
Size: 1586 248 3043
Fragments arranged by size:
3043 1586 248
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4877
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 1883 3548 3732 404
Size: 1479 1665 184 1549
Fragments arranged by size:
1665 1549 1479 184
MscI TGG'CCA
Cuts at: 2638 2638
Size: 4877
MslI CAynn'nnrTG
Cuts at: 1454 2866 2993 1454
Size: 1412 127 3338
Fragments arranged by size:
3338 1412 127
MspA1I CmG'CkG
Cuts at: 259 438 744 939 1110 1206 1988 2302 2662
Size: 179 306 195 171 96 782 314 360
Cuts at: 2662 3675 3920 259
Size: 1013 245 1216
Fragments arranged by size:
1216 1013 782 360 314 306 245 195
179 171 96
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4877
NarI GG'CG_CC
Cuts at: 722 2308 2556 722
Size: 1586 248 3043
Fragments arranged by size:
3043 1586 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4877
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4877
NsiI A_TGCA'T
Cuts at: 1369 1369
Size: 4877
NspI r_CATG'y
Cuts at: 852 1371 1930 2430 3337 852
Size: 519 559 500 907 2392
Fragments arranged by size:
2392 907 559 519 500
PciI A'CATG_T
Cuts at: 3333 3333
Size: 4877
PfoI T'CCnGG_A
Cuts at: 127 511 758 854 1034 1049 127
Size: 384 247 96 180 15 3955
Fragments arranged by size:
3955 384 247 180 96 15
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4845
PpuMI rG'GwC_Cy
Cuts at: 1053 1065 1053
Size: 12 4865
PspOMI G'GGCC_C
Cuts at: 177 177
Size: 4877
PspXI vC'TCGA_Gb
Cuts at: 748 748
Size: 4877
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 367 399 367
Size: 32 4845
PstI C_TGCA'G
Cuts at: 120 944 1241 1924 2609 120
Size: 824 297 683 685 2388
Fragments arranged by size:
2388 824 685 683 297
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 259 438 744 939 1206 2302 2662 259
Size: 179 306 195 267 1096 360 2474
Fragments arranged by size:
2474 1096 360 306 267 195 179
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 809 1891 809
Size: 1082 3795
SalI G'TCGA_C
Cuts at: 1914 1914
Size: 4877
SapI GCTCTTCn'nnn_
Cuts at: 814 2900 3110 814
Size: 2086 210 2581
Fragments arranged by size:
2581 2086 210
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4877
ScaI AGT'ACT
Cuts at: 1663 4394 1663
Size: 2731 2146
SexAI A'CCwGG_T
Cuts at: 1055 1055
Size: 4877
SfcI C'TryA_G
Cuts at: 33 116 940 1237 1920 2605 3598 3789 33
Size: 83 824 297 683 685 993 191 1121
Fragments arranged by size:
1121 993 824 685 683 297 191 83
SfoI GGC'GCC
Cuts at: 723 2309 2557 723
Size: 1586 248 3043
Fragments arranged by size:
3043 1586 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 543 571 1198 1651 1899 543
Size: 28 627 453 248 3521
Fragments arranged by size:
3521 627 453 248 28
SmlI C'TyrA_G
Cuts at: 703 748 946 2032 2947 3439 3701 703
Size: 45 198 1086 915 492 262 1879
Fragments arranged by size:
1879 1086 915 492 262 198 45
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 1371 1930 2430 1371
Size: 559 500 3818
Fragments arranged by size:
3818 559 500
StuI AGG'CCT
Cuts at: 682 682
Size: 4877
StyI C'CwwG_G
Cuts at: 107 206 896 1169 1589 1707 1766 2011 2365
Size: 99 690 273 420 118 59 245 354
Cuts at: 2365 107
Size: 2619
Fragments arranged by size:
2619 690 420 354 273 245 118 99
59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 835 1551 1615 2460 2593 3052 3261 835
Size: 716 64 845 133 459 209 2451
Fragments arranged by size:
2451 845 716 459 209 133 64
TatI w'GTAC_w
Cuts at: 1661 4392 1661
Size: 2731 2146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 4394 4426 607
Size: 32 3755 32 1058
Fragments arranged by size:
3755 1058 32 32
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 2372 189
Size: 2183 2694
XhoI C'TCGA_G
Cuts at: 748 748
Size: 4877
XmaI C'CCGG_G
Cuts at: 541 569 1196 1649 1897 541
Size: 28 627 453 248 3521
Fragments arranged by size:
3521 627 453 248 28
XmnI GAAnn'nnTTC
Cuts at: 1477 1609 1477
Size: 132 4745
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsgI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI