Restriction map for
pF1KB7894
(Circular) (Six-base) MAPSORT of: pF1KB7894.seq Check: 5907 from: 1 to: 5081
pF1KB7894 5081 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 1648 1648
Size: 5081
AccI GT'mk_AC
Cuts at: 2119 2119
Size: 5081
Acc65I G'GTAC_C
Cuts at: 2097 2097
Size: 5081
AclI AA'CG_TT
Cuts at: 4921 4921
Size: 5081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1423 2906 3338 4085 4353 1394
Size: 29 1483 432 747 268 2122
Fragments arranged by size:
2122 1483 747 432 268 29
AfeI AGC'GCT
Cuts at: 1618 1618
Size: 5081
AflIII A'CryG_T
Cuts at: 1593 2433 3537 4514 1593
Size: 840 1104 977 2160
Fragments arranged by size:
2160 1104 977 840
AgeI A'CCGG_T
Cuts at: 3469 3469
Size: 5081
AhdI GACnn_n'nnGTC
Cuts at: 159 1215 159
Size: 1056 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1408 1439 1408
Size: 31 5050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1442 1474 1442
Size: 32 5049
AlwNI CAG_nnn'CTG
Cuts at: 1233 1749 2386 3953 1233
Size: 516 637 1567 2361
Fragments arranged by size:
2361 1567 637 516
ApaI G_GGCC'C
Cuts at: 694 744 1241 694
Size: 50 497 4534
Fragments arranged by size:
4534 497 50
ApaLI G'TGCA_C
Cuts at: 3851 3851
Size: 5081
ApoI r'AATT_y
Cuts at: 2085 4434 4690 2085
Size: 2349 256 2476
Fragments arranged by size:
2476 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5081
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
AvaI C'yCGr_G
Cuts at: 413 506 743 1756 2101 413
Size: 93 237 1013 345 3393
Fragments arranged by size:
3393 1013 345 237 93
BamHI G'GATC_C
Cuts at: 966 2106 966
Size: 1140 3941
BanI G'GyrC_C
Cuts at: 1577 1802 2097 2511 2759 2794 1577
Size: 225 295 414 248 35 3864
Fragments arranged by size:
3864 414 295 248 225 35
BanII G_rGCy'C
Cuts at: 413 514 694 744 1241 1363 1832 2095 3125
Size: 101 180 50 497 122 469 263 1030
Cuts at: 3125 413
Size: 2369
Fragments arranged by size:
2369 1030 497 469 263 180 122 101
50
BbeI G_GCGC'C
Cuts at: 2515 2763 2515
Size: 248 4833
BciVI GTATCCnnnnn_n'
Cuts at: 2979 3740 5081 2979
Size: 761 1341 2979
Fragments arranged by size:
2979 1341 761
BclI T'GATC_A
Cuts at: 2601 2601
Size: 5081
BglI GCCn_nnn'nGGC
Cuts at: 816 816
Size: 5081
BglII A'GATC_T
Cuts at: 1556 2596 4633 1556
Size: 1040 2037 2004
Fragments arranged by size:
2037 2004 1040
BlpI GC'TnA_GC
Cuts at: 2193 2193
Size: 5081
Bme1580I G_kGCm'C
Cuts at: 694 744 1241 1428 1580 1756 1807 2706 2799
Size: 50 497 187 152 176 51 899 93
Cuts at: 2799 3855 694
Size: 1056 1920
Fragments arranged by size:
1920 1056 899 497 187 176 152 93
51 50
BmrI ACTGGGnnnn_n'
Cuts at: 1221 2293 2374 2709 4853 1221
Size: 1072 81 335 2144 1449
Fragments arranged by size:
2144 1449 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 960 3448 960
Size: 2488 2593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 1726 2046 122
Size: 1604 320 3157
Fragments arranged by size:
3157 1604 320
Bpu10I CC'TnA_GC
Cuts at: 935 935
Size: 5081
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2257 3136 3628 3926 1489
Size: 768 879 492 298 2644
Fragments arranged by size:
2644 879 768 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4785 4785
Size: 5081
BsaAI yAC'GTr
Cuts at: 1486 1594 1816 2434 1486
Size: 108 222 618 4133
Fragments arranged by size:
4133 618 222 108
BsaBI GATnn'nnATC
Cuts at: 6 2595 4632 6
Size: 2589 2037 455
Fragments arranged by size:
2589 2037 455
BsaHI Gr'CG_yC
Cuts at: 787 892 1645 2512 2760 3462 4958 787
Size: 105 753 867 248 702 1496 910
Fragments arranged by size:
1496 910 867 753 702 248 105
BsaWI w'CCGG_w
Cuts at: 207 272 2270 2791 3469 3743 3890 4301 207
Size: 65 1998 521 678 274 147 411 987
Fragments arranged by size:
1998 987 678 521 411 274 147 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1099 1129 2044 2074 1099
Size: 30 915 30 4106
Fragments arranged by size:
4106 915 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1147 1531 1147
Size: 384 4697
BseYI C'CCAG_C
Cuts at: 559 1259 1264 1334 1883 2506 3264 3841 4337
Size: 700 5 70 549 623 758 577 496
Cuts at: 4337 559
Size: 1303
Fragments arranged by size:
1303 758 700 623 577 549 496 70
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1013 2002 345
Size: 668 989 3424
Fragments arranged by size:
3424 989 668
BsiEI CG_ry'CG
Cuts at: 104 2073 2623 2669 3877 104
Size: 1969 550 46 1208 1308
Fragments arranged by size:
1969 1308 1208 550 46
BsiHKAI G_wGCw'C
Cuts at: 1382 1763 1832 2095 2873 3063 3855 1382
Size: 381 69 263 778 190 792 2608
Fragments arranged by size:
2608 792 778 381 263 190 69
BsmI GAATG_Cn'
Cuts at: 183 1403 183
Size: 1220 3861
Bsp1286I G_dGCh'C
Cuts at: 413 514 694 744 1241 1363 1382 1428 1580
Size: 101 180 50 497 122 19 46 152
Cuts at: 1580 1756 1763 1807 1832 2095 2706 2799 2873
Size: 176 7 44 25 263 611 93 74
Cuts at: 2873 3063 3125 3855 413
Size: 190 62 730 1639
Fragments arranged by size:
1639 730 611 497 263 190 180 176
152 122 101 93 74 62 50 46
44 25 19 7
BspEI T'CCGG_A
Cuts at: 272 4301 272
Size: 4029 1052
BspHI T'CATG_A
Cuts at: 224 4257 224
Size: 4033 1048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 1559 2063 2131 2345 2647 3028 467
Size: 1092 504 68 214 302 381 2520
Fragments arranged by size:
2520 1092 504 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 381 396 3373 3427 4911 5076 50
Size: 331 15 2977 54 1484 165 55
Fragments arranged by size:
2977 1484 331 165 55 54 15
BsrDI GCAATG_nn'
Cuts at: 252 396 1367 1974 2993 252
Size: 144 971 607 1019 2340
Fragments arranged by size:
2340 1019 971 607 144
BsrFI r'CCGG_y
Cuts at: 716 815 3079 3260 3469 716
Size: 99 2264 181 209 2328
Fragments arranged by size:
2328 2264 209 181 99
BssSI C'ACGA_G
Cuts at: 3352 3710 3352
Size: 358 4723
BstAPI GCAn_nnn'nTGC
Cuts at: 1503 2458 1503
Size: 955 4126
BstBI TT'CG_AA
Cuts at: 3442 3442
Size: 5081
BstYI r'GATC_y
Cuts at: 1 701 966 1325 1556 2106 2573 2596 2931
Size: 700 265 359 231 550 467 23 335
Cuts at: 2931 3177 4189 4275 4287 4633 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 700 550 467 449 359 346 335
265 246 231 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 917 917
Size: 5081
BtgI C'CryG_G
Cuts at: 107 258 1011 107
Size: 151 753 4177
Fragments arranged by size:
4177 753 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3031 3212 3375 3031
Size: 181 163 4737
Fragments arranged by size:
4737 181 163
BtsI GCAGTG_nn'
Cuts at: 411 820 893 1085 1219 1354 2324 411
Size: 409 73 192 134 135 970 3168
Fragments arranged by size:
3168 970 409 192 135 134 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1656 1691 1656
Size: 35 5046
DraI TTT'AAA
Cuts at: 2080 2080
Size: 5081
DrdI GACnn_nn'nnGTC
Cuts at: 2787 3645 2787
Size: 858 4223
EaeI y'GGCC_r
Cuts at: 290 551 817 2666 2840 3231 3258 4603 290
Size: 261 266 1849 174 391 27 1345 768
Fragments arranged by size:
1849 1345 768 391 266 261 174 27
EagI C'GGCC_G
Cuts at: 2666 2666
Size: 5081
EarI CTCTTCn'nnn_
Cuts at: 1034 1066 1370 1699 3104 3314 1034
Size: 32 304 329 1405 210 2801
Fragments arranged by size:
2801 1405 329 304 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3289 3599 3745 4891 3289
Size: 310 146 1146 3479
Fragments arranged by size:
3479 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1830 2093 1830
Size: 263 4818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 1394 1423 1726 2046 2906 3338 4085 4353
Size: 1272 29 303 320 860 432 747 268
Cuts at: 4353 122
Size: 850
Fragments arranged by size:
1272 860 850 747 432 320 303 268
29
EcoNI CCTnn'n_nnAGG
Cuts at: 979 979
Size: 5081
EcoO109I rG'GnC_Cy
Cuts at: 690 691 809 954 998 1209 1237 1770 2220
Size: 1 118 145 44 211 28 533 450
Cuts at: 2220 690
Size: 3551
Fragments arranged by size:
3551 533 450 211 145 118 44 28
1
EcoRI G'AATT_C
Cuts at: 2085 2085
Size: 5081
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 5081
FspI TGC'GCA
Cuts at: 1975 2862 1975
Size: 887 4194
FspAI rTGC'GCAy
Cuts at: 1975 1975
Size: 5081
HaeII r_GCGC'y
Cuts at: 468 1620 1863 2515 2763 3785 4764 468
Size: 1152 243 652 248 1022 979 785
Fragments arranged by size:
1152 1022 979 785 652 248 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 342 374 1099 1131 1201 1233 2044
Size: 32 165 32 725 32 70 32 811
Cuts at: 2044 2076 2923 2953 2955 2985 145
Size: 32 847 30 2 30 2241
Fragments arranged by size:
2241 847 811 725 165 70 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2120 2120
Size: 5081
KasI G'GCGC_C
Cuts at: 2511 2759 2511
Size: 248 4833
KpnI G_GTAC'C
Cuts at: 2101 2101
Size: 5081
MluI A'CGCG_T
Cuts at: 4514 4514
Size: 5081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3752 3936 3752
Size: 184 4897
MscI TGG'CCA
Cuts at: 292 553 2842 292
Size: 261 2289 2531
Fragments arranged by size:
2531 2289 261
MslI CAynn'nnrTG
Cuts at: 1981 3070 3197 1981
Size: 1089 127 3865
Fragments arranged by size:
3865 1089 127
MspA1I CmG'CkG
Cuts at: 735 1786 2192 2506 2866 3879 4124 735
Size: 1051 406 314 360 1013 245 1692
Fragments arranged by size:
1692 1051 1013 406 360 314 245
NaeI GCC'GGC
Cuts at: 718 817 3262 718
Size: 99 2445 2537
Fragments arranged by size:
2537 2445 99
NarI GG'CG_CC
Cuts at: 2512 2760 2512
Size: 248 4833
NcoI C'CATG_G
Cuts at: 107 258 1011 107
Size: 151 753 4177
Fragments arranged by size:
4177 753 151
NgoMIV G'CCGG_C
Cuts at: 716 815 3260 716
Size: 99 2445 2537
Fragments arranged by size:
2537 2445 99
NspI r_CATG'y
Cuts at: 612 632 1096 2134 2634 3541 612
Size: 20 464 1038 500 907 2152
Fragments arranged by size:
2152 1038 907 500 464 20
PciI A'CATG_T
Cuts at: 3537 3537
Size: 5081
PflMI CCAn_nnn'nTGG
Cuts at: 1233 1233
Size: 5081
PfoI T'CCnGG_A
Cuts at: 302 1052 302
Size: 750 4331
PmeI GTTT'AAAC
Cuts at: 2080 2080
Size: 5081
PmlI CAC'GTG
Cuts at: 1594 1594
Size: 5081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 4220
Size: 32 5049
PpuMI rG'GwC_Cy
Cuts at: 954 1209 1770 954
Size: 255 561 4265
Fragments arranged by size:
4265 561 255
PshAI GACnn'nnGTC
Cuts at: 650 650
Size: 5081
PspOMI G'GGCC_C
Cuts at: 690 740 1237 690
Size: 50 497 4534
Fragments arranged by size:
4534 497 50
PstI C_TGCA'G
Cuts at: 464 1556 2128 2813 464
Size: 1092 572 685 2732
Fragments arranged by size:
2732 1092 685 572
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5081
PvuII CAG'CTG
Cuts at: 735 1786 2506 2866 735
Size: 1051 720 360 2950
Fragments arranged by size:
2950 1051 720 360
RsrII CG'GwC_CG
Cuts at: 3276 3276
Size: 5081
SacI G_AGCT'C
Cuts at: 1832 2095 1832
Size: 263 4818
SalI G'TCGA_C
Cuts at: 2118 2118
Size: 5081
SanDI GG'GwC_CC
Cuts at: 1770 1770
Size: 5081
SapI GCTCTTCn'nnn_
Cuts at: 1370 3104 3314 1370
Size: 1734 210 3137
Fragments arranged by size:
3137 1734 210
SbfI CC_TGCA'GG
Cuts at: 2128 2128
Size: 5081
ScaI AGT'ACT
Cuts at: 760 4598 760
Size: 3838 1243
SfcI C'TryA_G
Cuts at: 33 460 923 1552 2124 2809 3802 3993 33
Size: 427 463 629 572 685 993 191 1121
Fragments arranged by size:
1121 993 685 629 572 463 427 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 816 816
Size: 5081
SfoI GGC'GCC
Cuts at: 2513 2761 2513
Size: 248 4833
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
SmaI CCC'GGG
Cuts at: 415 745 2103 415
Size: 330 1358 3393
Fragments arranged by size:
3393 1358 330
SmlI C'TyrA_G
Cuts at: 1468 2236 3151 3643 3905 1468
Size: 768 915 492 262 2644
Fragments arranged by size:
2644 915 768 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5081
SphI G_CATG'C
Cuts at: 2134 2634 2134
Size: 500 4581
StuI AGG'CCT
Cuts at: 441 441
Size: 5081
StyI C'CwwG_G
Cuts at: 107 258 293 545 805 1011 1205 2215 2569
Size: 151 35 252 260 206 194 1010 354
Cuts at: 2569 107
Size: 2619
Fragments arranged by size:
2619 1010 354 260 252 206 194 151
35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 844 1202 2664 2797 3256 3465 573
Size: 271 358 1462 133 459 209 2189
Fragments arranged by size:
2189 1462 459 358 271 209 133
TatI w'GTAC_w
Cuts at: 758 4596 758
Size: 3838 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 5049
Tth111I GACn'n_nGTC
Cuts at: 2878 2878
Size: 5081
XbaI T'CTAG_A
Cuts at: 2112 2112
Size: 5081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1648 2576 1648
Size: 928 4153
XmaI C'CCGG_G
Cuts at: 413 743 2101 413
Size: 330 1358 3393
Fragments arranged by size:
3393 1358 330
XmnI GAAnn'nnTTC
Cuts at: 682 1403 682
Size: 721 4360
ZraI GAC'GTC
Cuts at: 1646 1646
Size: 5081
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AleI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI DraIII EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspXI PsrI SacII SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI