Restriction map for
pF1KB7904
(Circular) (Six-base) MAPSORT of: pF1KB7904.seq Check: 5847 from: 1 to: 4097
pF1KB7904 4097 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 705 1135 705
Size: 430 3667
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 127 3937 127
Size: 3810 287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 401 876 1922 2354 3101 3369 362
Size: 39 475 1046 432 747 268 1090
Fragments arranged by size:
1090 1046 747 475 432 268 39
AflIII A'CryG_T
Cuts at: 440 1449 2553 3530 440
Size: 1009 1104 977 1007
Fragments arranged by size:
1104 1009 1007 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 1402 2969 1402
Size: 1567 2530
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 691 736 1101 3450 3706 691
Size: 45 365 2349 256 1082
Fragments arranged by size:
2349 1082 365 256 45
AseI AT'TA_AT
Cuts at: 20 320 20
Size: 300 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 1117 1117
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 1111 2141 1111
Size: 1030 3067
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 432 509 432
Size: 77 4020
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglII A'GATC_T
Cuts at: 384 1612 3649 384
Size: 1228 2037 832
Fragments arranged by size:
2037 1228 832
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 1722 1815 2871 1722
Size: 93 1056 2948
Fragments arranged by size:
2948 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
Bpu10I CC'TnA_GC
Cuts at: 551 551
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 353 383 353
Size: 30 4067
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 946 1111 1889 2079 2871 946
Size: 165 778 190 792 2172
Fragments arranged by size:
2172 792 778 190 165
Bsp1286I G_dGCh'C
Cuts at: 946 1111 1722 1815 1889 2079 2141 2871 946
Size: 165 611 93 74 190 62 730 2172
Fragments arranged by size:
2172 730 611 190 165 93 74 62
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 273 3273 273
Size: 3000 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 1147 1361 1663 2044 230
Size: 917 214 302 381 2283
Fragments arranged by size:
2283 917 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 602 797 2009 602
Size: 195 1212 2690
Fragments arranged by size:
2690 1212 195
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstEII G'GTnAC_C
Cuts at: 870 870
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 1030 1030
Size: 4097
BstYI r'GATC_y
Cuts at: 1 384 840 1122 1589 1612 1947 2193 3205
Size: 383 456 282 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 456 449 383 346 335 282
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 989 2047 2228 2391 989
Size: 1058 181 163 2695
Fragments arranged by size:
2695 1058 181 163
BtsI GCAGTG_nn'
Cuts at: 220 944 1340 220
Size: 724 396 2977
Fragments arranged by size:
2977 724 396
ClaI AT'CG_AT
Cuts at: 482 482
Size: 4097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 495 530 495
Size: 35 4062
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 1682 1856 2247 2274 3619 1682
Size: 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 275 854 2120 2330 275
Size: 579 1266 210 2042
Fragments arranged by size:
2042 1266 579 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 401 876 1922 2354 3101 3369 362
Size: 39 475 1046 432 747 268 1090
Fragments arranged by size:
1090 1046 747 475 432 268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1062 1062
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 885 1236 885
Size: 351 3746
EcoRI G'AATT_C
Cuts at: 691 1101 691
Size: 410 3687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 403 1878 403
Size: 1475 2622
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 1939 1969 1971 2001 445
Size: 32 1462 30 2 30 2541
Fragments arranged by size:
2541 1462 32 30 30 2
HincII GTy'rAC
Cuts at: 706 1136 706
Size: 430 3667
HindIII A'AGCT_T
Cuts at: 728 728
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 731 2768 2952 731
Size: 2037 184 1876
Fragments arranged by size:
2037 1876 184
MscI TGG'CCA
Cuts at: 1858 1858
Size: 4097
MslI CAynn'nnrTG
Cuts at: 974 2086 2213 974
Size: 1112 127 2858
Fragments arranged by size:
2858 1112 127
MspA1I CmG'CkG
Cuts at: 354 1208 1522 1882 2895 3140 354
Size: 854 314 360 1013 245 1311
Fragments arranged by size:
1311 1013 854 360 314 245
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 107
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 444 1150 1650 2557 444
Size: 706 500 907 1984
Fragments arranged by size:
1984 907 706 500
PacI TTA_AT'TAA
Cuts at: 320 320
Size: 4097
PciI A'CATG_T
Cuts at: 440 2553 440
Size: 2113 1984
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 885 885
Size: 4097
PstI C_TGCA'G
Cuts at: 760 951 1144 1829 760
Size: 191 193 685 3028
Fragments arranged by size:
3028 685 193 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 354 1522 1882 354
Size: 1168 360 2569
Fragments arranged by size:
2569 1168 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 704 1134 704
Size: 430 3667
SanDI GG'GwC_CC
Cuts at: 885 885
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 854 2120 2330 854
Size: 1266 210 2621
Fragments arranged by size:
2621 1266 210
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 167 756 947 1017 1140 1825 2818 3009
Size: 134 589 191 70 123 685 993 191
Cuts at: 3009 33
Size: 1121
Fragments arranged by size:
1121 993 685 589 191 191 134 123
70
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StuI AGG'CCT
Cuts at: 267 267
Size: 4097
StyI C'CwwG_G
Cuts at: 107 875 1231 1585 107
Size: 768 356 354 2619
Fragments arranged by size:
2619 768 356 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
XmnI GAAnn'nnTTC
Cuts at: 386 386
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PacI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI