Restriction map for
pF1KB7905
(Circular) (Six-base) MAPSORT of: pF1KB7905.seq Check: 1314 from: 1 to: 3485
pF1KB7905 3485 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 523 523
Size: 3485
Acc65I G'GTAC_C
Cuts at: 501 501
Size: 3485
AclI AA'CG_TT
Cuts at: 3325 3325
Size: 3485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1742 2489 2757 1310
Size: 432 747 268 2038
Fragments arranged by size:
2038 747 432 268
AflIII A'CryG_T
Cuts at: 837 1941 2918 837
Size: 1104 977 1404
Fragments arranged by size:
1404 1104 977
AgeI A'CCGG_T
Cuts at: 1873 1873
Size: 3485
AlwNI CAG_nnn'CTG
Cuts at: 790 2357 790
Size: 1567 1918
ApaLI G'TGCA_C
Cuts at: 2255 2255
Size: 3485
ApoI r'AATT_y
Cuts at: 489 2838 3094 489
Size: 2349 256 880
Fragments arranged by size:
2349 880 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3485
AvaI C'yCGr_G
Cuts at: 505 505
Size: 3485
BamHI G'GATC_C
Cuts at: 510 510
Size: 3485
BanI G'GyrC_C
Cuts at: 501 915 1163 1198 501
Size: 414 248 35 2788
Fragments arranged by size:
2788 414 248 35
BanII G_rGCy'C
Cuts at: 499 1529 499
Size: 1030 2455
BbeI G_GCGC'C
Cuts at: 919 1167 919
Size: 248 3237
BbsI GAAGACnn'nnnn_
Cuts at: 131 342 360 131
Size: 211 18 3256
Fragments arranged by size:
3256 211 18
BciVI GTATCCnnnnn_n'
Cuts at: 1383 2144 3485 1383
Size: 761 1341 1383
Fragments arranged by size:
1383 1341 761
BclI T'GATC_A
Cuts at: 1005 1005
Size: 3485
BglII A'GATC_T
Cuts at: 282 1000 3037 282
Size: 718 2037 730
Fragments arranged by size:
2037 730 718
BlpI GC'TnA_GC
Cuts at: 597 597
Size: 3485
Bme1580I G_kGCm'C
Cuts at: 1110 1203 2259 1110
Size: 93 1056 2336
Fragments arranged by size:
2336 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 697 778 1113 3257 697
Size: 81 335 2144 925
Fragments arranged by size:
2144 925 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1540 2032 2330 661
Size: 879 492 298 1816
Fragments arranged by size:
1816 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3189 3189
Size: 3485
BsaAI yAC'GTr
Cuts at: 838 838
Size: 3485
BsaBI GATnn'nnATC
Cuts at: 6 999 3036 6
Size: 993 2037 455
Fragments arranged by size:
2037 993 455
BsaHI Gr'CG_yC
Cuts at: 916 1164 1866 3362 916
Size: 248 702 1496 1039
Fragments arranged by size:
1496 1039 702 248
BsaWI w'CCGG_w
Cuts at: 674 1195 1873 2147 2294 2705 674
Size: 521 678 274 147 411 1454
Fragments arranged by size:
1454 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 176 176
Size: 3485
BseYI C'CCAG_C
Cuts at: 910 1668 2245 2741 910
Size: 758 577 496 1654
Fragments arranged by size:
1654 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 188
Size: 3485
BsiEI CG_ry'CG
Cuts at: 104 1027 1073 2281 104
Size: 923 46 1208 1308
Fragments arranged by size:
1308 1208 923 46
BsiHKAI G_wGCw'C
Cuts at: 499 1277 1467 2259 499
Size: 778 190 792 1725
Fragments arranged by size:
1725 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 499 1110 1203 1277 1467 1529 2259 499
Size: 611 93 74 190 62 730 1725
Fragments arranged by size:
1725 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2705 2705
Size: 3485
BspHI T'CATG_A
Cuts at: 2661 2661
Size: 3485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 749 1051 1432 535
Size: 214 302 381 2588
Fragments arranged by size:
2588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1777 1831 3315 3480 50
Size: 1727 54 1484 165 55
Fragments arranged by size:
1727 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 329 1397 329
Size: 1068 2417
BsrFI r'CCGG_y
Cuts at: 1483 1664 1873 1483
Size: 181 209 3095
Fragments arranged by size:
3095 209 181
BssSI C'ACGA_G
Cuts at: 1756 2114 1756
Size: 358 3127
BstAPI GCAn_nnn'nTGC
Cuts at: 862 862
Size: 3485
BstBI TT'CG_AA
Cuts at: 378 1846 378
Size: 1468 2017
BstYI r'GATC_y
Cuts at: 1 282 475 510 977 1000 1335 1581 2593
Size: 281 193 35 467 23 335 246 1012
Cuts at: 2593 2679 2691 3037 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 281 246 193
86 35 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 231 1435 1616 1779 231
Size: 1204 181 163 1937
Fragments arranged by size:
1937 1204 181 163
BtsI GCAGTG_nn'
Cuts at: 728 728
Size: 3485
DraI TTT'AAA
Cuts at: 484 484
Size: 3485
DrdI GACnn_nn'nnGTC
Cuts at: 1191 2049 1191
Size: 858 2627
EaeI y'GGCC_r
Cuts at: 1070 1244 1635 1662 3007 1070
Size: 174 391 27 1345 1548
Fragments arranged by size:
1548 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1070 1070
Size: 3485
EarI CTCTTCn'nnn_
Cuts at: 1508 1718 1508
Size: 210 3275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1693 2003 2149 3295 1693
Size: 310 146 1146 1883
Fragments arranged by size:
1883 1146 310 146
EcoICRI GAG'CTC
Cuts at: 497 497
Size: 3485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1310 1742 2489 2757 1310
Size: 432 747 268 2038
Fragments arranged by size:
2038 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 384 624 384
Size: 240 3245
EcoRI G'AATT_C
Cuts at: 489 489
Size: 3485
EcoRV GAT'ATC
Cuts at: 363 363
Size: 3485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 433 1504 433
Size: 1071 2414
FspI TGC'GCA
Cuts at: 1266 1266
Size: 3485
HaeII r_GCGC'y
Cuts at: 919 1167 2189 3168 919
Size: 248 1022 979 1236
Fragments arranged by size:
1236 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1327 1357 1359 1389 1327
Size: 30 2 30 3423
Fragments arranged by size:
3423 30 30 2
HincII GTy'rAC
Cuts at: 524 524
Size: 3485
KasI G'GCGC_C
Cuts at: 915 1163 915
Size: 248 3237
KpnI G_GTAC'C
Cuts at: 505 505
Size: 3485
MfeI C'AATT_G
Cuts at: 329 329
Size: 3485
MluI A'CGCG_T
Cuts at: 2918 2918
Size: 3485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2340 2156
Size: 184 3301
MscI TGG'CCA
Cuts at: 1246 1246
Size: 3485
MslI CAynn'nnrTG
Cuts at: 307 1474 1601 307
Size: 1167 127 2191
Fragments arranged by size:
2191 1167 127
MspA1I CmG'CkG
Cuts at: 596 910 1270 2283 2528 596
Size: 314 360 1013 245 1553
Fragments arranged by size:
1553 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1666 1666
Size: 3485
NarI GG'CG_CC
Cuts at: 916 1164 916
Size: 248 3237
NcoI C'CATG_G
Cuts at: 107 107
Size: 3485
NgoMIV G'CCGG_C
Cuts at: 1664 1664
Size: 3485
NspI r_CATG'y
Cuts at: 538 1038 1945 538
Size: 500 907 2078
Fragments arranged by size:
2078 907 500
PciI A'CATG_T
Cuts at: 1941 1941
Size: 3485
PmeI GTTT'AAAC
Cuts at: 484 484
Size: 3485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2624 2656 2624
Size: 32 3453
PpuMI rG'GwC_Cy
Cuts at: 384 384
Size: 3485
PstI C_TGCA'G
Cuts at: 532 1217 532
Size: 685 2800
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3485
PvuII CAG'CTG
Cuts at: 910 1270 910
Size: 360 3125
RsrII CG'GwC_CG
Cuts at: 1680 1680
Size: 3485
SacI G_AGCT'C
Cuts at: 499 499
Size: 3485
SalI G'TCGA_C
Cuts at: 522 522
Size: 3485
SapI GCTCTTCn'nnn_
Cuts at: 1508 1718 1508
Size: 210 3275
SbfI CC_TGCA'GG
Cuts at: 532 532
Size: 3485
ScaI AGT'ACT
Cuts at: 3002 3002
Size: 3485
SfcI C'TryA_G
Cuts at: 33 528 1213 2206 2397 33
Size: 495 685 993 191 1121
Fragments arranged by size:
1121 993 685 495 191
SfoI GGC'GCC
Cuts at: 917 1165 917
Size: 248 3237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3485
SmaI CCC'GGG
Cuts at: 507 507
Size: 3485
SmlI C'TyrA_G
Cuts at: 640 1555 2047 2309 640
Size: 915 492 262 1816
Fragments arranged by size:
1816 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3485
SphI G_CATG'C
Cuts at: 538 1038 538
Size: 500 2985
StyI C'CwwG_G
Cuts at: 107 388 619 973 107
Size: 281 231 354 2619
Fragments arranged by size:
2619 354 281 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1068 1201 1660 1869 1068
Size: 133 459 209 2684
Fragments arranged by size:
2684 459 209 133
TatI w'GTAC_w
Cuts at: 348 3000 348
Size: 2652 833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 3453
Tth111I GACn'n_nGTC
Cuts at: 341 1282 341
Size: 941 2544
XbaI T'CTAG_A
Cuts at: 516 516
Size: 3485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 980 980
Size: 3485
XmaI C'CCGG_G
Cuts at: 505 505
Size: 3485
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI