(Circular) (Six-base) MAPSORT of: pF1KB7905.seq Check: 1314 from: 1 to: 3485 pF1KB7905 3485 bp With 182 enzymes: SgfI * March 2, 2010 14:27 .. AccI GT'mk_AC Cuts at: 523 523 Size: 3485 Acc65I G'GTAC_C Cuts at: 501 501 Size: 3485 AclI AA'CG_TT Cuts at: 3325 3325 Size: 3485 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1310 1742 2489 2757 1310 Size: 432 747 268 2038 Fragments arranged by size: 2038 747 432 268 AflIII A'CryG_T Cuts at: 837 1941 2918 837 Size: 1104 977 1404 Fragments arranged by size: 1404 1104 977 AgeI A'CCGG_T Cuts at: 1873 1873 Size: 3485 AlwNI CAG_nnn'CTG Cuts at: 790 2357 790 Size: 1567 1918 ApaLI G'TGCA_C Cuts at: 2255 2255 Size: 3485 ApoI r'AATT_y Cuts at: 489 2838 3094 489 Size: 2349 256 880 Fragments arranged by size: 2349 880 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3485 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3485 AvaI C'yCGr_G Cuts at: 505 505 Size: 3485 BamHI G'GATC_C Cuts at: 510 510 Size: 3485 BanI G'GyrC_C Cuts at: 501 915 1163 1198 501 Size: 414 248 35 2788 Fragments arranged by size: 2788 414 248 35 BanII G_rGCy'C Cuts at: 499 1529 499 Size: 1030 2455 BbeI G_GCGC'C Cuts at: 919 1167 919 Size: 248 3237 BbsI GAAGACnn'nnnn_ Cuts at: 131 342 360 131 Size: 211 18 3256 Fragments arranged by size: 3256 211 18 BciVI GTATCCnnnnn_n' Cuts at: 1383 2144 3485 1383 Size: 761 1341 1383 Fragments arranged by size: 1383 1341 761 BclI T'GATC_A Cuts at: 1005 1005 Size: 3485 BglII A'GATC_T Cuts at: 282 1000 3037 282 Size: 718 2037 730 Fragments arranged by size: 2037 730 718 BlpI GC'TnA_GC Cuts at: 597 597 Size: 3485 Bme1580I G_kGCm'C Cuts at: 1110 1203 2259 1110 Size: 93 1056 2336 Fragments arranged by size: 2336 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 697 778 1113 3257 697 Size: 81 335 2144 925 Fragments arranged by size: 2144 925 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1852 1852 Size: 3485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 661 1540 2032 2330 661 Size: 879 492 298 1816 Fragments arranged by size: 1816 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3189 3189 Size: 3485 BsaAI yAC'GTr Cuts at: 838 838 Size: 3485 BsaBI GATnn'nnATC Cuts at: 6 999 3036 6 Size: 993 2037 455 Fragments arranged by size: 2037 993 455 BsaHI Gr'CG_yC Cuts at: 916 1164 1866 3362 916 Size: 248 702 1496 1039 Fragments arranged by size: 1496 1039 702 248 BsaWI w'CCGG_w Cuts at: 674 1195 1873 2147 2294 2705 674 Size: 521 678 274 147 411 1454 Fragments arranged by size: 1454 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 176 176 Size: 3485 BseYI C'CCAG_C Cuts at: 910 1668 2245 2741 910 Size: 758 577 496 1654 Fragments arranged by size: 1654 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 188 188 Size: 3485 BsiEI CG_ry'CG Cuts at: 104 1027 1073 2281 104 Size: 923 46 1208 1308 Fragments arranged by size: 1308 1208 923 46 BsiHKAI G_wGCw'C Cuts at: 499 1277 1467 2259 499 Size: 778 190 792 1725 Fragments arranged by size: 1725 792 778 190 Bsp1286I G_dGCh'C Cuts at: 499 1110 1203 1277 1467 1529 2259 499 Size: 611 93 74 190 62 730 1725 Fragments arranged by size: 1725 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2705 2705 Size: 3485 BspHI T'CATG_A Cuts at: 2661 2661 Size: 3485 BspMI ACCTGCnnnn'nnnn_ Cuts at: 535 749 1051 1432 535 Size: 214 302 381 2588 Fragments arranged by size: 2588 381 302 214 BsrBI CCG'CTC Cuts at: 50 1777 1831 3315 3480 50 Size: 1727 54 1484 165 55 Fragments arranged by size: 1727 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 329 1397 329 Size: 1068 2417 BsrFI r'CCGG_y Cuts at: 1483 1664 1873 1483 Size: 181 209 3095 Fragments arranged by size: 3095 209 181 BssSI C'ACGA_G Cuts at: 1756 2114 1756 Size: 358 3127 BstAPI GCAn_nnn'nTGC Cuts at: 862 862 Size: 3485 BstBI TT'CG_AA Cuts at: 378 1846 378 Size: 1468 2017 BstYI r'GATC_y Cuts at: 1 282 475 510 977 1000 1335 1581 2593 Size: 281 193 35 467 23 335 246 1012 Cuts at: 2593 2679 2691 3037 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 346 335 281 246 193 86 35 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3485 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 231 1435 1616 1779 231 Size: 1204 181 163 1937 Fragments arranged by size: 1937 1204 181 163 BtsI GCAGTG_nn' Cuts at: 728 728 Size: 3485 DraI TTT'AAA Cuts at: 484 484 Size: 3485 DrdI GACnn_nn'nnGTC Cuts at: 1191 2049 1191 Size: 858 2627 EaeI y'GGCC_r Cuts at: 1070 1244 1635 1662 3007 1070 Size: 174 391 27 1345 1548 Fragments arranged by size: 1548 1345 391 174 27 EagI C'GGCC_G Cuts at: 1070 1070 Size: 3485 EarI CTCTTCn'nnn_ Cuts at: 1508 1718 1508 Size: 210 3275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1693 2003 2149 3295 1693 Size: 310 146 1146 1883 Fragments arranged by size: 1883 1146 310 146 EcoICRI GAG'CTC Cuts at: 497 497 Size: 3485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1310 1742 2489 2757 1310 Size: 432 747 268 2038 Fragments arranged by size: 2038 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 384 624 384 Size: 240 3245 EcoRI G'AATT_C Cuts at: 489 489 Size: 3485 EcoRV GAT'ATC Cuts at: 363 363 Size: 3485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 433 1504 433 Size: 1071 2414 FspI TGC'GCA Cuts at: 1266 1266 Size: 3485 HaeII r_GCGC'y Cuts at: 919 1167 2189 3168 919 Size: 248 1022 979 1236 Fragments arranged by size: 1236 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1327 1357 1359 1389 1327 Size: 30 2 30 3423 Fragments arranged by size: 3423 30 30 2 HincII GTy'rAC Cuts at: 524 524 Size: 3485 KasI G'GCGC_C Cuts at: 915 1163 915 Size: 248 3237 KpnI G_GTAC'C Cuts at: 505 505 Size: 3485 MfeI C'AATT_G Cuts at: 329 329 Size: 3485 MluI A'CGCG_T Cuts at: 2918 2918 Size: 3485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2156 2340 2156 Size: 184 3301 MscI TGG'CCA Cuts at: 1246 1246 Size: 3485 MslI CAynn'nnrTG Cuts at: 307 1474 1601 307 Size: 1167 127 2191 Fragments arranged by size: 2191 1167 127 MspA1I CmG'CkG Cuts at: 596 910 1270 2283 2528 596 Size: 314 360 1013 245 1553 Fragments arranged by size: 1553 1013 360 314 245 NaeI GCC'GGC Cuts at: 1666 1666 Size: 3485 NarI GG'CG_CC Cuts at: 916 1164 916 Size: 248 3237 NcoI C'CATG_G Cuts at: 107 107 Size: 3485 NgoMIV G'CCGG_C Cuts at: 1664 1664 Size: 3485 NspI r_CATG'y Cuts at: 538 1038 1945 538 Size: 500 907 2078 Fragments arranged by size: 2078 907 500 PciI A'CATG_T Cuts at: 1941 1941 Size: 3485 PmeI GTTT'AAAC Cuts at: 484 484 Size: 3485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2624 2656 2624 Size: 32 3453 PpuMI rG'GwC_Cy Cuts at: 384 384 Size: 3485 PstI C_TGCA'G Cuts at: 532 1217 532 Size: 685 2800 PvuI CG_AT'CG Cuts at: 104 104 Size: 3485 PvuII CAG'CTG Cuts at: 910 1270 910 Size: 360 3125 RsrII CG'GwC_CG Cuts at: 1680 1680 Size: 3485 SacI G_AGCT'C Cuts at: 499 499 Size: 3485 SalI G'TCGA_C Cuts at: 522 522 Size: 3485 SapI GCTCTTCn'nnn_ Cuts at: 1508 1718 1508 Size: 210 3275 SbfI CC_TGCA'GG Cuts at: 532 532 Size: 3485 ScaI AGT'ACT Cuts at: 3002 3002 Size: 3485 SfcI C'TryA_G Cuts at: 33 528 1213 2206 2397 33 Size: 495 685 993 191 1121 Fragments arranged by size: 1121 993 685 495 191 SfoI GGC'GCC Cuts at: 917 1165 917 Size: 248 3237 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3485 SmaI CCC'GGG Cuts at: 507 507 Size: 3485 SmlI C'TyrA_G Cuts at: 640 1555 2047 2309 640 Size: 915 492 262 1816 Fragments arranged by size: 1816 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3485 SphI G_CATG'C Cuts at: 538 1038 538 Size: 500 2985 StyI C'CwwG_G Cuts at: 107 388 619 973 107 Size: 281 231 354 2619 Fragments arranged by size: 2619 354 281 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1068 1201 1660 1869 1068 Size: 133 459 209 2684 Fragments arranged by size: 2684 459 209 133 TatI w'GTAC_w Cuts at: 348 3000 348 Size: 2652 833 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3002 3034 3002 Size: 32 3453 Tth111I GACn'n_nGTC Cuts at: 341 1282 341 Size: 941 2544 XbaI T'CTAG_A Cuts at: 516 516 Size: 3485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 980 980 Size: 3485 XmaI C'CCGG_G Cuts at: 505 505 Size: 3485 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI