Restriction map for
pF1KB7911
(Circular) (Six-base) MAPSORT of: pF1KB7911.seq Check: 6342 from: 1 to: 3554
pF1KB7911 3554 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 542 592 542
Size: 50 3504
Acc65I G'GTAC_C
Cuts at: 570 570
Size: 3554
AclI AA'CG_TT
Cuts at: 548 3394 548
Size: 2846 708
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1811 2558 2826 1379
Size: 432 747 268 2107
Fragments arranged by size:
2107 747 432 268
AflIII A'CryG_T
Cuts at: 906 2010 2987 906
Size: 1104 977 1473
Fragments arranged by size:
1473 1104 977
AgeI A'CCGG_T
Cuts at: 1942 1942
Size: 3554
AlwNI CAG_nnn'CTG
Cuts at: 859 2426 859
Size: 1567 1987
ApaLI G'TGCA_C
Cuts at: 2324 2324
Size: 3554
ApoI r'AATT_y
Cuts at: 558 2907 3163 558
Size: 2349 256 949
Fragments arranged by size:
2349 949 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
AvaI C'yCGr_G
Cuts at: 121 574 121
Size: 453 3101
BamHI G'GATC_C
Cuts at: 579 579
Size: 3554
BanI G'GyrC_C
Cuts at: 570 984 1232 1267 570
Size: 414 248 35 2857
Fragments arranged by size:
2857 414 248 35
BanII G_rGCy'C
Cuts at: 568 1598 568
Size: 1030 2524
BbeI G_GCGC'C
Cuts at: 988 1236 988
Size: 248 3306
BbsI GAAGACnn'nnnn_
Cuts at: 228 298 500 228
Size: 70 202 3282
Fragments arranged by size:
3282 202 70
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2213 3554 1452
Size: 761 1341 1452
Fragments arranged by size:
1452 1341 761
BclI T'GATC_A
Cuts at: 149 1074 149
Size: 925 2629
BglII A'GATC_T
Cuts at: 1069 3106 1069
Size: 2037 1517
BlpI GC'TnA_GC
Cuts at: 666 666
Size: 3554
Bme1580I G_kGCm'C
Cuts at: 1179 1272 2328 1179
Size: 93 1056 2405
Fragments arranged by size:
2405 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 766 847 1182 3326 766
Size: 81 335 2144 994
Fragments arranged by size:
2144 994 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1609 2101 2399 730
Size: 879 492 298 1885
Fragments arranged by size:
1885 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3258 3258
Size: 3554
BsaAI yAC'GTr
Cuts at: 907 907
Size: 3554
BsaBI GATnn'nnATC
Cuts at: 6 1068 3105 6
Size: 1062 2037 455
Fragments arranged by size:
2037 1062 455
BsaHI Gr'CG_yC
Cuts at: 985 1233 1935 3431 985
Size: 248 702 1496 1108
Fragments arranged by size:
1496 1108 702 248
BsaWI w'CCGG_w
Cuts at: 743 1264 1942 2216 2363 2774 743
Size: 521 678 274 147 411 1523
Fragments arranged by size:
1523 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 979 1737 2314 2810 979
Size: 758 577 496 1723
Fragments arranged by size:
1723 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1096 1142 2350 104
Size: 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 46
BsiHKAI G_wGCw'C
Cuts at: 317 568 1346 1536 2328 317
Size: 251 778 190 792 1543
Fragments arranged by size:
1543 792 778 251 190
BsmI GAATG_Cn'
Cuts at: 306 306
Size: 3554
Bsp1286I G_dGCh'C
Cuts at: 317 568 1179 1272 1346 1536 1598 2328 317
Size: 251 611 93 74 190 62 730 1543
Fragments arranged by size:
1543 730 611 251 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2774 2774
Size: 3554
BspHI T'CATG_A
Cuts at: 2730 2730
Size: 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 143 604 818 1120 1501 143
Size: 461 214 302 381 2196
Fragments arranged by size:
2196 461 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1846 1900 3384 3549 50
Size: 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 281 1466 281
Size: 1185 2369
BsrFI r'CCGG_y
Cuts at: 1552 1733 1942 1552
Size: 181 209 3164
Fragments arranged by size:
3164 209 181
BssSI C'ACGA_G
Cuts at: 1825 2183 1825
Size: 358 3196
BstAPI GCAn_nnn'nTGC
Cuts at: 931 931
Size: 3554
BstBI TT'CG_AA
Cuts at: 1915 1915
Size: 3554
BstYI r'GATC_y
Cuts at: 1 579 1046 1069 1404 1650 2662 2748 2760
Size: 578 467 23 335 246 1012 86 12
Cuts at: 2760 3106 1
Size: 346 449
Fragments arranged by size:
1012 578 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 323 107
Size: 216 3338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1504 1685 1848 1504
Size: 181 163 3210
Fragments arranged by size:
3210 181 163
BtsI GCAGTG_nn'
Cuts at: 797 797
Size: 3554
DraI TTT'AAA
Cuts at: 553 553
Size: 3554
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2118 1260
Size: 858 2696
EaeI y'GGCC_r
Cuts at: 1139 1313 1704 1731 3076 1139
Size: 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1139 1139
Size: 3554
EarI CTCTTCn'nnn_
Cuts at: 323 452 524 1577 1787 323
Size: 129 72 1053 210 2090
Fragments arranged by size:
2090 1053 210 129 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1762 2072 2218 3364 127
Size: 1635 310 146 1146 317
Fragments arranged by size:
1635 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 566 566
Size: 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 1811 2558 2826 1379
Size: 432 747 268 2107
Fragments arranged by size:
2107 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 126 693 126
Size: 567 2987
EcoRI G'AATT_C
Cuts at: 558 558
Size: 3554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 1573
Size: 3554
FspI TGC'GCA
Cuts at: 1335 1335
Size: 3554
HaeII r_GCGC'y
Cuts at: 175 215 988 1236 2258 3237 175
Size: 40 773 248 1022 979 492
Fragments arranged by size:
1022 979 773 492 248 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 1396 1426 1428 1458 301
Size: 32 1063 30 2 30 2397
Fragments arranged by size:
2397 1063 32 30 30 2
HincII GTy'rAC
Cuts at: 189 593 189
Size: 404 3150
KasI G'GCGC_C
Cuts at: 984 1232 984
Size: 248 3306
KpnI G_GTAC'C
Cuts at: 574 574
Size: 3554
MluI A'CGCG_T
Cuts at: 2987 2987
Size: 3554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2409 2225
Size: 184 3370
MscI TGG'CCA
Cuts at: 1315 1315
Size: 3554
MslI CAynn'nnrTG
Cuts at: 1543 1670 1543
Size: 127 3427
MspA1I CmG'CkG
Cuts at: 345 665 979 1339 2352 2597 345
Size: 320 314 360 1013 245 1302
Fragments arranged by size:
1302 1013 360 320 314 245
NaeI GCC'GGC
Cuts at: 1735 1735
Size: 3554
NarI GG'CG_CC
Cuts at: 985 1233 985
Size: 248 3306
NcoI C'CATG_G
Cuts at: 107 323 107
Size: 216 3338
NgoMIV G'CCGG_C
Cuts at: 1733 1733
Size: 3554
NspI r_CATG'y
Cuts at: 607 1107 2014 607
Size: 500 907 2147
Fragments arranged by size:
2147 907 500
PciI A'CATG_T
Cuts at: 2010 2010
Size: 3554
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 3554
PmeI GTTT'AAAC
Cuts at: 553 553
Size: 3554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 354 2693 2725 322
Size: 32 2339 32 1151
Fragments arranged by size:
2339 1151 32 32
PpuMI rG'GwC_Cy
Cuts at: 126 126
Size: 3554
PstI C_TGCA'G
Cuts at: 601 1286 601
Size: 685 2869
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3554
PvuII CAG'CTG
Cuts at: 979 1339 979
Size: 360 3194
RsrII CG'GwC_CG
Cuts at: 1749 1749
Size: 3554
SacI G_AGCT'C
Cuts at: 568 568
Size: 3554
SalI G'TCGA_C
Cuts at: 591 591
Size: 3554
SapI GCTCTTCn'nnn_
Cuts at: 452 1577 1787 452
Size: 1125 210 2219
Fragments arranged by size:
2219 1125 210
SbfI CC_TGCA'GG
Cuts at: 601 601
Size: 3554
ScaI AGT'ACT
Cuts at: 3071 3071
Size: 3554
SfcI C'TryA_G
Cuts at: 33 597 1282 2275 2466 33
Size: 564 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 191
SfoI GGC'GCC
Cuts at: 986 1234 986
Size: 248 3306
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3554
SmaI CCC'GGG
Cuts at: 576 576
Size: 3554
SmlI C'TyrA_G
Cuts at: 709 1624 2116 2378 709
Size: 915 492 262 1885
Fragments arranged by size:
1885 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3554
SphI G_CATG'C
Cuts at: 607 1107 607
Size: 500 3054
StuI AGG'CCT
Cuts at: 412 412
Size: 3554
StyI C'CwwG_G
Cuts at: 107 197 323 688 1042 107
Size: 90 126 365 354 2619
Fragments arranged by size:
2619 365 354 126 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1270 1729 1938 1137
Size: 133 459 209 2753
Fragments arranged by size:
2753 459 209 133
TatI w'GTAC_w
Cuts at: 3069 3069
Size: 3554
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 3522
Tth111I GACn'n_nGTC
Cuts at: 184 1351 184
Size: 1167 2387
XbaI T'CTAG_A
Cuts at: 585 585
Size: 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1049 330
Size: 719 2835
XmaI C'CCGG_G
Cuts at: 574 574
Size: 3554
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI