Restriction map for
pF1KB7916
(Circular) (Six-base) MAPSORT of: pF1KB7916.seq Check: 6614 from: 1 to: 4148
pF1KB7916 4148 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 1052 1052
Size: 4148
AccI GT'mk_AC
Cuts at: 878 1186 878
Size: 308 3840
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1973 2405 3152 3420 446
Size: 1527 432 747 268 1174
Fragments arranged by size:
1527 1174 747 432 268
AfeI AGC'GCT
Cuts at: 942 942
Size: 4148
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 843 2536 843
Size: 1693 2455
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaI G_GGCC'C
Cuts at: 434 434
Size: 4148
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 1152 3501 3757 1152
Size: 2349 256 1543
Fragments arranged by size:
2349 1543 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 299 536 1168 299
Size: 237 632 3279
Fragments arranged by size:
3279 632 237
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 338 371 338
Size: 33 4115
BamHI G'GATC_C
Cuts at: 272 1173 272
Size: 901 3247
BanI G'GyrC_C
Cuts at: 1121 1164 1578 1826 1861 1121
Size: 43 414 248 35 3408
Fragments arranged by size:
3408 414 248 43 35
BanII G_rGCy'C
Cuts at: 299 434 582 860 980 1162 2192 299
Size: 135 148 278 120 182 1030 2255
Fragments arranged by size:
2255 1030 278 182 148 135 120
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BbsI GAAGACnn'nnnn_
Cuts at: 335 335
Size: 4148
BbvCI CC'TCA_GC
Cuts at: 652 652
Size: 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 271 305 90
Size: 34 147 34 3933
Fragments arranged by size:
3933 147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglI GCCn_nnn'nGGC
Cuts at: 619 1099 1130 619
Size: 480 31 3637
Fragments arranged by size:
3637 480 31
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 434 1773 1866 2922 434
Size: 1339 93 1056 1660
Fragments arranged by size:
1660 1339 1056 93
BmgBI CAC'GTC
Cuts at: 913 913
Size: 4148
BmrI ACTGGGnnnn_n'
Cuts at: 928 1360 1441 1776 3920 928
Size: 432 81 335 2144 1156
Fragments arranged by size:
2144 1156 432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 842 2515 842
Size: 1673 2475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 333
Size: 4148
Bpu10I CC'TnA_GC
Cuts at: 652 652
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 398 3852 398
Size: 3454 694
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1049 1579 1827 2529 4025 1049
Size: 530 248 702 1496 1172
Fragments arranged by size:
1496 1172 702 530 248
BsaWI w'CCGG_w
Cuts at: 843 1337 1858 2536 2810 2957 3368 843
Size: 494 521 678 274 147 411 1623
Fragments arranged by size:
1623 678 521 494 411 274 147
BseYI C'CCAG_C
Cuts at: 245 668 1573 2331 2908 3404 245
Size: 423 905 758 577 496 989
Fragments arranged by size:
989 905 758 577 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 1004 177
Size: 827 3321
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1162 1940 2130 2922 1162
Size: 778 190 792 2388
Fragments arranged by size:
2388 792 778 190
BsmI GAATG_Cn'
Cuts at: 1133 1133
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 299 434 582 860 980 1162 1773 1866 1940
Size: 135 148 278 120 182 611 93 74
Cuts at: 1940 2130 2192 2922 299
Size: 190 62 730 1525
Fragments arranged by size:
1525 730 611 278 190 182 148 135
120 93 74 62
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1412 1714 2095 1198
Size: 214 302 381 3251
Fragments arranged by size:
3251 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 843 2146 2327 2536 843
Size: 1303 181 209 2455
Fragments arranged by size:
2455 1303 209 181
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 272 1173 1640 1663 1998 2244 3256 3342
Size: 271 901 467 23 335 246 1012 86
Cuts at: 3342 3354 3700 1
Size: 12 346 449
Fragments arranged by size:
1012 901 467 449 346 335 271 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 491 491
Size: 4148
BtgI C'CryG_G
Cuts at: 517 947 517
Size: 430 3718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DraIII CAC_nnn'GTG
Cuts at: 795 795
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 316 917 1854 2712 316
Size: 601 937 858 1752
Fragments arranged by size:
1752 937 858 601
EaeI y'GGCC_r
Cuts at: 915 1733 1907 2298 2325 3670 915
Size: 818 174 391 27 1345 1393
Fragments arranged by size:
1393 1345 818 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 1007 2171 2381 1007
Size: 1164 210 2774
Fragments arranged by size:
2774 1164 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 817 2356 2666 2812 3958 817
Size: 1539 310 146 1146 1007
Fragments arranged by size:
1539 1146 1007 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 446 1973 2405 3152 3420 333
Size: 113 1527 432 747 268 1061
Fragments arranged by size:
1527 1061 747 432 268 113
EcoNI CCTnn'n_nnAGG
Cuts at: 1006 1006
Size: 4148
EcoO109I rG'GnC_Cy
Cuts at: 430 431 1287 430
Size: 1 856 3291
Fragments arranged by size:
3291 856 1
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 133 1929 133
Size: 1796 2352
HaeII r_GCGC'y
Cuts at: 944 1582 1830 2852 3831 944
Size: 638 248 1022 979 1261
Fragments arranged by size:
1261 1022 979 638 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1990 2020 2022 2052 1990
Size: 30 2 30 4086
Fragments arranged by size:
4086 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 390 495 2819 3003 147
Size: 243 105 2324 184 1292
Fragments arranged by size:
2324 1292 243 184 105
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 2137 2264 2137
Size: 127 4021
MspA1I CmG'CkG
Cuts at: 588 1259 1573 1933 2946 3191 588
Size: 671 314 360 1013 245 1545
Fragments arranged by size:
1545 1013 671 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 947 947
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 292 1201 1701 2608 292
Size: 909 500 907 1832
Fragments arranged by size:
1832 909 907 500
PasI CC'CwG_GG
Cuts at: 573 1117 573
Size: 544 3604
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PspOMI G'GGCC_C
Cuts at: 430 430
Size: 4148
PstI C_TGCA'G
Cuts at: 963 1081 1195 1880 963
Size: 118 114 685 3231
Fragments arranged by size:
3231 685 118 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 1573 1933 1573
Size: 360 3788
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 959 1077 1191 1876 2869 3060 33
Size: 926 118 114 685 993 191 1121
Fragments arranged by size:
1121 993 926 685 191 118 114
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 301 538 1170 301
Size: 237 632 3279
Fragments arranged by size:
3279 632 237
SmlI C'TyrA_G
Cuts at: 1303 2218 2710 2972 1303
Size: 915 492 262 2479
Fragments arranged by size:
2479 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 292 1201 1701 292
Size: 909 500 2739
Fragments arranged by size:
2739 909 500
StyI C'CwwG_G
Cuts at: 342 617 947 1282 1636 342
Size: 275 330 335 354 2854
Fragments arranged by size:
2854 354 335 330 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1731 1864 2323 2532 1731
Size: 133 459 209 3347
Fragments arranged by size:
3347 459 209 133
TatI w'GTAC_w
Cuts at: 3663 3663
Size: 4148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 253 1643 253
Size: 1390 2758
XmaI C'CCGG_G
Cuts at: 299 536 1168 299
Size: 237 632 3279
Fragments arranged by size:
3279 632 237
ZraI GAC'GTC
Cuts at: 1050 1050
Size: 4148
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BfrBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI