Restriction map for
pF1KB7918
(Circular) (Six-base) MAPSORT of: pF1KB7918.seq Check: 8877 from: 1 to: 4151
pF1KB7918 4151 bp
With 182 enzymes: SgfI *
November 10, 2008 10:17 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 709 1090 1976 2408 3155 3423 540
Size: 169 381 886 432 747 268 1268
Fragments arranged by size:
1268 886 747 432 381 268 169
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AhdI GACnn_n'nnGTC
Cuts at: 365 365
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 517 1456 3023 517
Size: 939 1567 1645
Fragments arranged by size:
1645 1567 939
ApaI G_GGCC'C
Cuts at: 490 490
Size: 4151
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 123 184 483 800 1171 123
Size: 61 299 317 371 3103
Fragments arranged by size:
3103 371 317 299 61
BamHI G'GATC_C
Cuts at: 132 941 1176 132
Size: 809 235 3107
Fragments arranged by size:
3107 809 235
BanI G'GyrC_C
Cuts at: 397 1135 1167 1581 1829 1864 397
Size: 738 32 414 248 35 2684
Fragments arranged by size:
2684 738 414 248 35 32
BanII G_rGCy'C
Cuts at: 490 822 1046 1098 1165 2195 490
Size: 332 224 52 67 1030 2446
Fragments arranged by size:
2446 1030 332 224 67 52
BbeI G_GCGC'C
Cuts at: 401 1585 1833 401
Size: 1184 248 2719
Fragments arranged by size:
2719 1184 248
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 490 509 1138 1776 1869 2925 490
Size: 19 629 638 93 1056 1716
Fragments arranged by size:
1716 1056 638 629 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 1138 704
Size: 434 3717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 997 3855 997
Size: 2858 1293
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 200 398 709 1582 1830 2532 4028 200
Size: 198 311 873 248 702 1496 323
Fragments arranged by size:
1496 873 702 323 311 248 198
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 320 350 320
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 253 825 1078 208
Size: 45 572 253 3281
Fragments arranged by size:
3281 572 253 45
BseYI C'CCAG_C
Cuts at: 476 618 821 1097 1576 2334 2911 3407 476
Size: 142 203 276 479 758 577 496 1220
Fragments arranged by size:
1220 758 577 496 479 276 203 142
BsiEI CG_ry'CG
Cuts at: 104 581 749 1693 1739 2947 104
Size: 477 168 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 477 168 46
BsiHKAI G_wGCw'C
Cuts at: 1046 1165 1943 2133 2925 1046
Size: 119 778 190 792 2272
Fragments arranged by size:
2272 792 778 190 119
Bsp1286I G_dGCh'C
Cuts at: 490 509 822 1046 1098 1138 1165 1776 1869
Size: 19 313 224 52 40 27 611 93
Cuts at: 1869 1943 2133 2195 2925 490
Size: 74 190 62 730 1716
Fragments arranged by size:
1716 730 611 313 224 190 93 74
62 52 40 27 19
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 701 3327 701
Size: 2626 1525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1134 1201 1415 1717 2098 1134
Size: 67 214 302 381 3187
Fragments arranged by size:
3187 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 235 840 1023 2443 2497 3981 4146 50
Size: 185 605 183 1420 54 1484 165 55
Fragments arranged by size:
1484 1420 605 185 183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 567 2063 567
Size: 1496 2655
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 607 1528 607
Size: 921 3230
BstBI TT'CG_AA
Cuts at: 883 2512 883
Size: 1629 2522
BstYI r'GATC_y
Cuts at: 1 132 941 1176 1643 1666 2001 2247 3259
Size: 131 809 235 467 23 335 246 1012
Cuts at: 3259 3345 3357 3703 1
Size: 86 12 346 449
Fragments arranged by size:
1012 809 467 449 346 335 246 235
131 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1048 1048
Size: 4151
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 146 254 378 578 1736 1910 2301 2328 3673
Size: 108 124 200 1158 174 391 27 1345
Cuts at: 3673 146
Size: 624
Fragments arranged by size:
1345 1158 624 391 200 174 124 108
27
EagI C'GGCC_G
Cuts at: 578 1736 578
Size: 1158 2993
EarI CTCTTCn'nnn_
Cuts at: 810 2174 2384 810
Size: 1364 210 2577
Fragments arranged by size:
2577 1364 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 205 861 2359 2669 2815 3961 205
Size: 656 1498 310 146 1146 395
Fragments arranged by size:
1498 1146 656 395 310 146
EcoICRI GAG'CTC
Cuts at: 1044 1163 1044
Size: 119 4032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 704 709 1090 1138 1976 2408 3155 3423
Size: 164 5 381 48 838 432 747 268
Cuts at: 3423 540
Size: 1268
Fragments arranged by size:
1268 838 747 432 381 268 164 48
5
EcoNI CCTnn'n_nnAGG
Cuts at: 949 949
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 181 487 514 1290 181
Size: 306 27 776 3042
Fragments arranged by size:
3042 776 306 27
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 401 483 526 1585 1833 2855 3834 401
Size: 82 43 1059 248 1022 979 718
Fragments arranged by size:
1059 1022 979 718 248 82 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 534 566 1993 2023 2025 2055 351
Size: 32 151 32 1427 30 2 30 2447
Fragments arranged by size:
2447 1427 151 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 397 1581 1829 397
Size: 1184 248 2719
Fragments arranged by size:
2719 1184 248
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 256 1912 256
Size: 1656 2495
MslI CAynn'nnrTG
Cuts at: 112 2140 2267 112
Size: 2028 127 1996
Fragments arranged by size:
2028 1996 127
MspA1I CmG'CkG
Cuts at: 139 154 250 372 394 421 496 534 593
Size: 15 96 122 22 27 75 38 59
Cuts at: 593 1101 1262 1576 1936 2949 3194 139
Size: 508 161 314 360 1013 245 1096
Fragments arranged by size:
1096 1013 508 360 314 245 161 122
96 75 59 38 27 22 15
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 398 1582 1830 398
Size: 1184 248 2719
Fragments arranged by size:
2719 1184 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 117 999 1204 1704 2611 117
Size: 882 205 500 907 1657
Fragments arranged by size:
1657 907 882 500 205
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 181 514 181
Size: 333 3818
PshAI GACnn'nnGTC
Cuts at: 125 125
Size: 4151
PspOMI G'GGCC_C
Cuts at: 486 486
Size: 4151
PstI C_TGCA'G
Cuts at: 212 522 1031 1106 1198 1883 212
Size: 310 509 75 92 685 2480
Fragments arranged by size:
2480 685 509 310 92 75
PvuI CG_AT'CG
Cuts at: 104 749 104
Size: 645 3506
PvuII CAG'CTG
Cuts at: 372 394 1101 1576 1936 372
Size: 22 707 475 360 2587
Fragments arranged by size:
2587 707 475 360 22
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1046 1165 1046
Size: 119 4032
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SanDI GG'GwC_CC
Cuts at: 181 181
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 810 2174 2384 810
Size: 1364 210 2577
Fragments arranged by size:
2577 1364 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 208 518 1027 1102 1194 1879 2872 3063
Size: 175 310 509 75 92 685 993 191
Cuts at: 3063 33
Size: 1121
Fragments arranged by size:
1121 993 685 509 310 191 175 92
75
SfoI GGC'GCC
Cuts at: 399 1583 1831 399
Size: 1184 248 2719
Fragments arranged by size:
2719 1184 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 125 485 1173 125
Size: 360 688 3103
Fragments arranged by size:
3103 688 360
SmlI C'TyrA_G
Cuts at: 1306 2221 2713 2975 1306
Size: 915 492 262 2482
Fragments arranged by size:
2482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 999 1204 1704 999
Size: 205 500 3446
Fragments arranged by size:
3446 500 205
SrfI GCCC'GGGC
Cuts at: 485 485
Size: 4151
StuI AGG'CCT
Cuts at: 955 955
Size: 4151
StyI C'CwwG_G
Cuts at: 107 950 1285 1639 107
Size: 843 335 354 2619
Fragments arranged by size:
2619 843 354 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 448 1734 1867 2326 2535 448
Size: 1286 133 459 209 2064
Fragments arranged by size:
2064 1286 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 320 352 3668 3700 320
Size: 32 3316 32 771
Fragments arranged by size:
3316 771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 361 1646 114
Size: 247 1285 2619
Fragments arranged by size:
2619 1285 247
XmaI C'CCGG_G
Cuts at: 123 483 1171 123
Size: 360 688 3103
Fragments arranged by size:
3103 688 360
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI