Restriction map for
pF1KB7919
(Circular) (Six-base) MAPSORT of: pF1KB7919.seq Check: 9633 from: 1 to: 3653
pF1KB7919 3653 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 958 2525 958
Size: 1567 2086
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 247 669 1083 1331 1366 247
Size: 422 414 248 35 2534
Fragments arranged by size:
2534 422 414 248 35
BanII G_rGCy'C
Cuts at: 127 667 1697 127
Size: 540 1030 2083
Fragments arranged by size:
2083 1030 540
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 141 141
Size: 3653
BglII A'GATC_T
Cuts at: 471 1168 3205 471
Size: 697 2037 919
Fragments arranged by size:
2037 919 697
BlpI GC'TnA_GC
Cuts at: 446 765 446
Size: 319 3334
Bme1580I G_kGCm'C
Cuts at: 250 1278 1371 2427 250
Size: 1028 93 1056 1476
Fragments arranged by size:
1476 1056 1028 93
BmrI ACTGGGnnnn_n'
Cuts at: 183 273 865 946 1281 3425 183
Size: 90 592 81 335 2144 411
Fragments arranged by size:
2144 592 411 335 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 461 2020 461
Size: 1559 2094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 197 842 1363 2041 2315 2462 2873 197
Size: 645 521 678 274 147 411 977
Fragments arranged by size:
977 678 645 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 629 629
Size: 3653
BseYI C'CCAG_C
Cuts at: 1078 1836 2413 2909 1078
Size: 758 577 496 1822
Fragments arranged by size:
1822 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 174
Size: 3653
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 447 667 1445 1635 2427 447
Size: 220 778 190 792 1673
Fragments arranged by size:
1673 792 778 220 190
Bsp1286I G_dGCh'C
Cuts at: 127 250 447 667 1278 1371 1445 1635 1697
Size: 123 197 220 611 93 74 190 62
Cuts at: 1697 2427 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 220 197 190 123 93
74 62
BspEI T'CCGG_A
Cuts at: 197 2873 197
Size: 2676 977
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 703 917 1219 1600 285
Size: 418 214 302 381 2338
Fragments arranged by size:
2338 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 230 312 529 1030 230
Size: 82 217 501 2853
Fragments arranged by size:
2853 501 217 82
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 471 678 1145 1168 1503 1749 2761 2847
Size: 470 207 467 23 335 246 1012 86
Cuts at: 2847 2859 3205 1
Size: 12 346 449
Fragments arranged by size:
1012 470 467 449 346 335 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 248 1603 1784 1947 248
Size: 1355 181 163 1954
Fragments arranged by size:
1954 1355 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 184 1359 2217 184
Size: 1175 858 1620
Fragments arranged by size:
1620 1175 858
EaeI y'GGCC_r
Cuts at: 1238 1412 1803 1830 3175 1238
Size: 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 115 1676 1886 115
Size: 1561 210 1882
Fragments arranged by size:
1882 1561 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 792 792
Size: 3653
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 209 1087 1335 2357 3336 209
Size: 878 248 1022 979 526
Fragments arranged by size:
1022 979 878 526 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 447 2324 2508 447
Size: 1877 184 1592
Fragments arranged by size:
1877 1592 184
MscI TGG'CCA
Cuts at: 1414 1414
Size: 3653
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 764 1078 1438 2451 2696 764
Size: 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PstI C_TGCA'G
Cuts at: 543 700 1385 543
Size: 157 685 2811
Fragments arranged by size:
2811 685 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 115 1676 1886 115
Size: 1561 210 1882
Fragments arranged by size:
1882 1561 210
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 180 539 696 1381 2374 2565 33
Size: 147 359 157 685 993 191 1121
Fragments arranged by size:
1121 993 685 359 191 157 147
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StuI AGG'CCT
Cuts at: 214 214
Size: 3653
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 624 1236 1369 1828 2037 624
Size: 612 133 459 209 2240
Fragments arranged by size:
2240 612 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI