Restriction map for
pF1KB7920
(Circular) (Six-base) MAPSORT of: pF1KB7920.seq Check: 3943 from: 1 to: 4151
pF1KB7920 4151 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 860 860
Size: 4151
AccI GT'mk_AC
Cuts at: 613 1189 613
Size: 576 3575
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 939 1976 2408 3155 3423 939
Size: 1037 432 747 268 1667
Fragments arranged by size:
1667 1037 747 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 1456 3023 1456
Size: 1567 2584
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 249 341 1155 3504 3760 249
Size: 92 814 2349 256 640
Fragments arranged by size:
2349 814 640 256 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1007 1171 1007
Size: 164 3987
BamHI G'GATC_C
Cuts at: 632 1176 632
Size: 544 3607
BanI G'GyrC_C
Cuts at: 357 1167 1581 1829 1864 357
Size: 810 414 248 35 2644
Fragments arranged by size:
2644 810 414 248 35
BanII G_rGCy'C
Cuts at: 443 839 1165 2195 443
Size: 396 326 1030 2399
Fragments arranged by size:
2399 1030 396 326
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbvCI CC'TCA_GC
Cuts at: 353 857 353
Size: 504 3647
BciVI GTATCCnnnnn_n'
Cuts at: 942 2049 2810 4151 942
Size: 1107 761 1341 942
Fragments arranged by size:
1341 1107 942 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 707 1263 707
Size: 556 3595
Bme1580I G_kGCm'C
Cuts at: 673 1776 1869 2925 673
Size: 1103 93 1056 1899
Fragments arranged by size:
1899 1103 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BmtI G_CTAG'C
Cuts at: 352 352
Size: 4151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 517 310
Size: 207 3944
Bpu10I CC'TnA_GC
Cuts at: 353 857 353
Size: 504 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1327 2206 2698 2996 853
Size: 474 879 492 298 2008
Fragments arranged by size:
2008 879 492 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 597 1504 597
Size: 907 3244
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 208 971 1340 1861 2539 2813 2960 3371 208
Size: 763 369 521 678 274 147 411 988
Fragments arranged by size:
988 763 678 521 411 369 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 874 874
Size: 4151
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 919
Size: 4151
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1943 2133 2925 1165
Size: 778 190 792 2391
Fragments arranged by size:
2391 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 535 535
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 443 673 839 1165 1776 1869 1943 2133 2195
Size: 230 166 326 611 93 74 190 62
Cuts at: 2195 2925 443
Size: 730 1669
Fragments arranged by size:
1669 730 611 326 230 190 166 93
74 62
BspEI T'CCGG_A
Cuts at: 971 3371 971
Size: 2400 1751
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 743 860 1201 1415 1717 2098 743
Size: 117 341 214 302 381 2796
Fragments arranged by size:
2796 381 341 302 214 117
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 180 320 2063 180
Size: 140 1743 2268
Fragments arranged by size:
2268 1743 140
BsrFI r'CCGG_y
Cuts at: 1106 2149 2330 2539 1106
Size: 1043 181 209 2718
Fragments arranged by size:
2718 1043 209 181
BsrGI T'GTAC_A
Cuts at: 389 389
Size: 4151
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 322 1528 322
Size: 1206 2945
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 632 1176 1643 1666 2001 2247 3259 3345
Size: 631 544 467 23 335 246 1012 86
Cuts at: 3345 3357 3703 1
Size: 12 346 449
Fragments arranged by size:
1012 631 544 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 614 614
Size: 4151
BtgI C'CryG_G
Cuts at: 107 839 107
Size: 732 3419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 687 2101 2282 2445 687
Size: 1414 181 163 2393
Fragments arranged by size:
2393 1414 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DraIII CAC_nnn'GTG
Cuts at: 609 886 609
Size: 277 3874
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 720 923 1736 1910 2301 2328 3673 720
Size: 203 813 174 391 27 1345 1198
Fragments arranged by size:
1345 1198 813 391 203 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 517 939 1976 2408 3155 3423 310
Size: 207 422 1037 432 747 268 1038
Fragments arranged by size:
1038 1037 747 432 422 268 207
EcoO109I rG'GnC_Cy
Cuts at: 381 1290 381
Size: 909 3242
EcoRI G'AATT_C
Cuts at: 341 1155 341
Size: 814 3337
EcoRV GAT'ATC
Cuts at: 696 696
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1113 1585 1833 2855 3834 1113
Size: 472 248 1022 979 1430
Fragments arranged by size:
1430 1022 979 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 411 443 501 533 1993 2023 2025
Size: 32 111 32 58 32 1460 30 2
Cuts at: 2025 2055 268
Size: 30 2364
Fragments arranged by size:
2364 1460 111 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1043 1190 1043
Size: 147 4004
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 702 2822 3006 702
Size: 2120 184 1847
Fragments arranged by size:
2120 1847 184
MscI TGG'CCA
Cuts at: 722 925 1912 722
Size: 203 987 2961
Fragments arranged by size:
2961 987 203
MslI CAynn'nnrTG
Cuts at: 275 2140 2267 275
Size: 1865 127 2159
Fragments arranged by size:
2159 1865 127
MspA1I CmG'CkG
Cuts at: 711 856 1262 1576 1936 2949 3194 711
Size: 145 406 314 360 1013 245 1668
Fragments arranged by size:
1668 1013 406 360 314 245 145
NaeI GCC'GGC
Cuts at: 1108 2332 1108
Size: 1224 2927
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NdeI CA'TA_TG
Cuts at: 623 623
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 1106 2330 1106
Size: 1224 2927
NheI G'CTAG_C
Cuts at: 348 348
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PasI CC'CwG_GG
Cuts at: 639 639
Size: 4151
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 381 381
Size: 4151
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 711 856 1576 1936 711
Size: 145 720 360 2926
Fragments arranged by size:
2926 720 360 145
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 1091 3668 1091
Size: 2577 1574
SexAI A'CCwGG_T
Cuts at: 882 882
Size: 4151
SfcI C'TryA_G
Cuts at: 33 520 1194 1879 2872 3063 33
Size: 487 674 685 993 191 1121
Fragments arranged by size:
1121 993 685 674 487 191
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1009 1173 1009
Size: 164 3987
SmlI C'TyrA_G
Cuts at: 832 1306 2221 2713 2975 832
Size: 474 915 492 262 2008
Fragments arranged by size:
2008 915 492 474 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 107 486 909 1285 1639 107
Size: 379 423 376 354 2619
Fragments arranged by size:
2619 423 379 376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 267 389 1089 3666 267
Size: 122 700 2577 752
Fragments arranged by size:
2577 752 700 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 942 1646 942
Size: 704 3447
XmaI C'CCGG_G
Cuts at: 1007 1171 1007
Size: 164 3987
XmnI GAAnn'nnTTC
Cuts at: 250 250
Size: 4151
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BsaXI
BsiWI BsmI BssHII BstEII BstXI Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI