Restriction map for
pF1KB7921
(Circular) (Six-base) MAPSORT of: pF1KB7921.seq Check: 5749 from: 1 to: 3656
pF1KB7921 3656 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 694 694
Size: 3656
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 511 1481 1913 2660 2928 377
Size: 134 970 432 747 268 1105
Fragments arranged by size:
1105 970 747 432 268 134
AflIII A'CryG_T
Cuts at: 562 1008 2112 3089 562
Size: 446 1104 977 1129
Fragments arranged by size:
1129 1104 977 446
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 304 961 2528 304
Size: 657 1567 1432
Fragments arranged by size:
1567 1432 657
ApaLI G'TGCA_C
Cuts at: 2426 2426
Size: 3656
ApoI r'AATT_y
Cuts at: 660 3009 3265 660
Size: 2349 256 1051
Fragments arranged by size:
2349 1051 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 322 676 322
Size: 354 3302
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 672 1086 1334 1369 672
Size: 414 248 35 2959
Fragments arranged by size:
2959 414 248 35
BanII G_rGCy'C
Cuts at: 512 579 670 1700 512
Size: 67 91 1030 2468
Fragments arranged by size:
2468 1030 91 67
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BbsI GAAGACnn'nnnn_
Cuts at: 442 442
Size: 3656
BbvCI CC'TCA_GC
Cuts at: 241 241
Size: 3656
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 768 768
Size: 3656
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2430 1281
Size: 93 1056 2507
Fragments arranged by size:
2507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 949 1284 3428 868
Size: 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 3656
Bpu10I CC'TnA_GC
Cuts at: 241 241
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 832 1711 2203 2501 496
Size: 336 879 492 298 1651
Fragments arranged by size:
1651 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 138 3360 138
Size: 3222 434
BsaAI yAC'GTr
Cuts at: 563 1009 563
Size: 446 3210
BsaBI GATnn'nnATC
Cuts at: 6 1170 3207 6
Size: 1164 2037 455
Fragments arranged by size:
2037 1164 455
BsaHI Gr'CG_yC
Cuts at: 220 1087 1335 2037 3533 220
Size: 867 248 702 1496 343
Fragments arranged by size:
1496 867 702 343 248
BsaWI w'CCGG_w
Cuts at: 275 845 1366 2044 2318 2465 2876 275
Size: 570 521 678 274 147 411 1055
Fragments arranged by size:
1055 678 570 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 520
Size: 3656
BseYI C'CCAG_C
Cuts at: 1081 1839 2416 2912 1081
Size: 758 577 496 1825
Fragments arranged by size:
1825 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1198 1244 2452 104
Size: 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 46
BsiHKAI G_wGCw'C
Cuts at: 512 670 1448 1638 2430 512
Size: 158 778 190 792 1738
Fragments arranged by size:
1738 792 778 190 158
BsmI GAATG_Cn'
Cuts at: 486 486
Size: 3656
Bsp1286I G_dGCh'C
Cuts at: 512 579 670 1281 1374 1448 1638 1700 2430
Size: 67 91 611 93 74 190 62 730
Cuts at: 2430 512
Size: 1738
Fragments arranged by size:
1738 730 611 190 93 91 74 67
62
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 2832 2832
Size: 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 920 1222 1603 706
Size: 214 302 381 2759
Fragments arranged by size:
2759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 640 1568 640
Size: 928 2728
BsrFI r'CCGG_y
Cuts at: 1654 1835 2044 1654
Size: 181 209 3266
Fragments arranged by size:
3266 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 681 1148 1171 1506 1752 2764 2850 2862
Size: 680 467 23 335 246 1012 86 12
Cuts at: 2862 3208 1
Size: 346 449
Fragments arranged by size:
1012 680 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 899 899
Size: 3656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 549 584 549
Size: 35 3621
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2220 1362
Size: 858 2798
EaeI y'GGCC_r
Cuts at: 1241 1415 1806 1833 3178 1241
Size: 174 391 27 1345 1719
Fragments arranged by size:
1719 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1241 1241
Size: 3656
EarI CTCTTCn'nnn_
Cuts at: 230 1679 1889 230
Size: 1449 210 1997
Fragments arranged by size:
1997 1449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1864 2174 2320 3466 1864
Size: 310 146 1146 2054
Fragments arranged by size:
2054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 510 668 510
Size: 158 3498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 511 578 1481 1913 2660 2928 377
Size: 134 67 903 432 747 268 1105
Fragments arranged by size:
1105 903 747 432 268 134 67
EcoO109I rG'GnC_Cy
Cuts at: 795 795
Size: 3656
EcoRI G'AATT_C
Cuts at: 660 660
Size: 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 1437 1437
Size: 3656
HaeII r_GCGC'y
Cuts at: 1090 1338 2360 3339 1090
Size: 248 1022 979 1407
Fragments arranged by size:
1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1498 1528 1530 1560 1498
Size: 30 2 30 3594
Fragments arranged by size:
3594 30 30 2
HincII GTy'rAC
Cuts at: 695 695
Size: 3656
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 645 2327 2511 440
Size: 205 1682 184 1585
Fragments arranged by size:
1682 1585 205 184
MscI TGG'CCA
Cuts at: 1417 1417
Size: 3656
MslI CAynn'nnrTG
Cuts at: 1645 1772 1645
Size: 127 3529
MspA1I CmG'CkG
Cuts at: 406 767 1081 1441 2454 2699 406
Size: 361 314 360 1013 245 1363
Fragments arranged by size:
1363 1013 361 360 314 245
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 543 709 1209 2116 543
Size: 166 500 907 2083
Fragments arranged by size:
2083 907 500 166
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PmlI CAC'GTG
Cuts at: 563 563
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PstI C_TGCA'G
Cuts at: 452 703 1388 452
Size: 251 685 2720
Fragments arranged by size:
2720 685 251
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 406 1081 1441 406
Size: 675 360 2621
Fragments arranged by size:
2621 675 360
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 512 670 512
Size: 158 3498
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 230 1679 1889 230
Size: 1449 210 1997
Fragments arranged by size:
1997 1449 210
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 3173 3173
Size: 3656
SfcI C'TryA_G
Cuts at: 33 448 699 1384 2377 2568 33
Size: 415 251 685 993 191 1121
Fragments arranged by size:
1121 993 685 415 251 191
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 511 811 1726 2218 2480 511
Size: 300 915 492 262 1687
Fragments arranged by size:
1687 915 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StyI C'CwwG_G
Cuts at: 790 1144 790
Size: 354 3302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 137 1239 1372 1831 2040 137
Size: 1102 133 459 209 1753
Fragments arranged by size:
1753 1102 459 209 133
TatI w'GTAC_w
Cuts at: 3171 3171
Size: 3656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 265 297 3173 3205 265
Size: 32 2876 32 716
Fragments arranged by size:
2876 716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1453 1453
Size: 3656
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI