Restriction map for
pF1KB7928
(Circular) (Six-base) MAPSORT of: pF1KB7928.seq Check: 9057 from: 1 to: 3713
pF1KB7928 3713 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 671 1538 1970 2717 2985 553
Size: 118 867 432 747 268 1281
Fragments arranged by size:
1281 867 747 432 268 118
AflIII A'CryG_T
Cuts at: 1065 2169 3146 1065
Size: 1104 977 1632
Fragments arranged by size:
1632 1104 977
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 1018 2585 1018
Size: 1567 2146
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 717 3066 3322 717
Size: 2349 256 1108
Fragments arranged by size:
2349 1108 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 225 682 733 225
Size: 457 51 3205
Fragments arranged by size:
3205 457 51
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 194 649 727 1757 194
Size: 455 78 1030 2150
Fragments arranged by size:
2150 1030 455 78
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BbsI GAAGACnn'nnnn_
Cuts at: 604 604
Size: 3713
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 359 1233 359
Size: 874 2839
BglII A'GATC_T
Cuts at: 1228 3265 1228
Size: 2037 1676
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 683 1338 1431 2487 683
Size: 655 93 1056 1909
Fragments arranged by size:
1909 1056 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 108 925 1006 1341 3485 108
Size: 817 81 335 2144 336
Fragments arranged by size:
2144 817 336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 582 2080 582
Size: 1498 2215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 643 178
Size: 465 3248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1768 2260 2558 889
Size: 879 492 298 2044
Fragments arranged by size:
2044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 1144 1392 2094 3590 1144
Size: 248 702 1496 1267
Fragments arranged by size:
1496 1267 702 248
BsaWI w'CCGG_w
Cuts at: 433 902 1423 2101 2375 2522 2933 433
Size: 469 521 678 274 147 411 1213
Fragments arranged by size:
1213 678 521 469 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 415 428 445 458 415
Size: 13 17 13 3670
Fragments arranged by size:
3670 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 532 532
Size: 3713
BseYI C'CCAG_C
Cuts at: 569 1138 1896 2473 2969 569
Size: 569 758 577 496 1313
Fragments arranged by size:
1313 758 577 569 496
BsiEI CG_ry'CG
Cuts at: 104 1255 1301 2509 104
Size: 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 46
BsiHKAI G_wGCw'C
Cuts at: 727 1505 1695 2487 727
Size: 778 190 792 1953
Fragments arranged by size:
1953 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 194 649 683 727 1338 1431 1505 1695 1757
Size: 455 34 44 611 93 74 190 62
Cuts at: 1757 2487 194
Size: 730 1420
Fragments arranged by size:
1420 730 611 455 190 93 74 62
44 34
BspEI T'CCGG_A
Cuts at: 433 2933 433
Size: 2500 1213
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 977 1279 1660 763
Size: 214 302 381 2816
Fragments arranged by size:
2816 381 302 214
BsrBI CCG'CTC
Cuts at: 50 134 2005 2059 3543 3708 50
Size: 84 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 1625 1625
Size: 3713
BsrFI r'CCGG_y
Cuts at: 333 1711 1892 2101 333
Size: 1378 181 209 1945
Fragments arranged by size:
1945 1378 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 164 1090 164
Size: 926 2787
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstXI CCAn_nnnn'nTGG
Cuts at: 187 447 483 187
Size: 260 36 3417
Fragments arranged by size:
3417 260 36
BstYI r'GATC_y
Cuts at: 1 480 527 542 738 1205 1228 1563 1809
Size: 479 47 15 196 467 23 335 246
Cuts at: 1809 2821 2907 2919 3265 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 479 467 449 346 335 246 196
86 47 23 15 12
BtgI C'CryG_G
Cuts at: 107 476 107
Size: 369 3344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1663 1844 2007 1663
Size: 181 163 3369
Fragments arranged by size:
3369 181 163
BtsI GCAGTG_nn'
Cuts at: 956 956
Size: 3713
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 449 1298 1472 1863 1890 3235 449
Size: 849 174 391 27 1345 927
Fragments arranged by size:
1345 927 849 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 421 647 1736 1946 421
Size: 226 1089 210 2188
Fragments arranged by size:
2188 1089 226 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 553 643 671 1538 1970 2717 2985 178
Size: 375 90 28 867 432 747 268 906
Fragments arranged by size:
906 867 747 432 375 268 90 28
EcoO109I rG'GnC_Cy
Cuts at: 345 687 852 345
Size: 342 165 3206
Fragments arranged by size:
3206 342 165
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 1732 291
Size: 1441 2272
FspI TGC'GCA
Cuts at: 1494 1494
Size: 3713
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 1555 1585 1587 1617 118
Size: 32 1405 30 2 30 2214
Fragments arranged by size:
2214 1405 32 30 30 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2568 2384
Size: 184 3529
MscI TGG'CCA
Cuts at: 451 1474 451
Size: 1023 2690
MslI CAynn'nnrTG
Cuts at: 185 1702 1829 185
Size: 1517 127 2069
Fragments arranged by size:
2069 1517 127
MspA1I CmG'CkG
Cuts at: 209 258 621 824 1138 1498 2511 2756 209
Size: 49 363 203 314 360 1013 245 1166
Fragments arranged by size:
1166 1013 363 360 314 245 203 49
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 476 107
Size: 369 3344
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 766 1266 2173 766
Size: 500 907 2306
Fragments arranged by size:
2306 907 500
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PpuMI rG'GwC_Cy
Cuts at: 345 345
Size: 3713
PstI C_TGCA'G
Cuts at: 263 760 1445 263
Size: 497 685 2531
Fragments arranged by size:
2531 685 497
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 258 621 1138 1498 258
Size: 363 517 360 2473
Fragments arranged by size:
2473 517 363 360
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SanDI GG'GwC_CC
Cuts at: 345 345
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 421 647 1736 1946 421
Size: 226 1089 210 2188
Fragments arranged by size:
2188 1089 226 210
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 236 259 756 1441 2434 2625 33
Size: 203 23 497 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 203 191 23
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 227 735 227
Size: 508 3205
SmlI C'TyrA_G
Cuts at: 868 1783 2275 2537 868
Size: 915 492 262 2044
Fragments arranged by size:
2044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3713
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
StyI C'CwwG_G
Cuts at: 107 476 847 1201 107
Size: 369 371 354 2619
Fragments arranged by size:
2619 371 369 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1296 1429 1888 2097 1296
Size: 133 459 209 2912
Fragments arranged by size:
2912 459 209 133
TatI w'GTAC_w
Cuts at: 3228 3228
Size: 3713
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 428 447 460 3230 3262 415
Size: 13 19 13 2770 32 866
Fragments arranged by size:
2770 866 32 19 13 13
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1208 1208
Size: 3713
XmaI C'CCGG_G
Cuts at: 225 733 225
Size: 508 3205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI