Restriction map for
pF1KB7934
(Circular) (Six-base) MAPSORT of: pF1KB7934.seq Check: 2752 from: 1 to: 3758
pF1KB7934 3758 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 796 796
Size: 3758
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 3598 3598
Size: 3758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 580 1583 2015 2762 3030 194
Size: 386 1003 432 747 268 922
Fragments arranged by size:
1003 922 747 432 386 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 221 221
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 283 762 3111 3367 283
Size: 479 2349 256 674
Fragments arranged by size:
2349 674 479 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 378 783 378
Size: 405 3353
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 772 1802 772
Size: 1030 2728
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BbsI GAAGACnn'nnnn_
Cuts at: 262 262
Size: 3758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 157 191 157
Size: 34 3724
BciVI GTATCCnnnnn_n'
Cuts at: 1656 2417 3758 1656
Size: 761 1341 1656
Fragments arranged by size:
1656 1341 761
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BfrBI ATG'CAT
Cuts at: 157 157
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 1383 1476 2532 1383
Size: 93 1056 2609
Fragments arranged by size:
2609 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 502 970 1051 1386 3530 502
Size: 468 81 335 2144 730
Fragments arranged by size:
2144 730 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 682 712 715 718 196
Size: 486 30 3 3 3236
Fragments arranged by size:
3236 486 30 3 3
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 244 772 1550 1740 2532 244
Size: 528 778 190 792 1470
Fragments arranged by size:
1470 792 778 528 190
Bsp1286I G_dGCh'C
Cuts at: 244 772 1383 1476 1550 1740 1802 2532 244
Size: 528 611 93 74 190 62 730 1470
Fragments arranged by size:
1470 730 611 528 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1135
Size: 3758
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 120 378 783 1250 1273 1608 1854 2866
Size: 119 258 405 467 23 335 246 1012
Cuts at: 2866 2952 2964 3310 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 405 346 335 258 246
119 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3758
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 512 1001 512
Size: 489 3269
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 1343 1517 1908 1935 3280 1343
Size: 174 391 27 1345 1821
Fragments arranged by size:
1821 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 424 659 683 1781 1991 424
Size: 235 24 1098 210 2191
Fragments arranged by size:
2191 1098 235 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 580 1583 2015 2762 3030 194
Size: 386 1003 432 747 268 922
Fragments arranged by size:
1003 922 747 432 386 268
EcoNI CCTnn'n_nnAGG
Cuts at: 350 350
Size: 3758
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1630 1632 1662 1600
Size: 30 2 30 3696
Fragments arranged by size:
3696 30 30 2
HincII GTy'rAC
Cuts at: 216 797 216
Size: 581 3177
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 1519 1519
Size: 3758
MslI CAynn'nnrTG
Cuts at: 203 1747 1874 203
Size: 1544 127 2087
Fragments arranged by size:
2087 1544 127
MspA1I CmG'CkG
Cuts at: 869 1183 1543 2556 2801 869
Size: 314 360 1013 245 1826
Fragments arranged by size:
1826 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NcoI C'CATG_G
Cuts at: 107 107
Size: 3758
NdeI CA'TA_TG
Cuts at: 153 153
Size: 3758
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NsiI A_TGCA'T
Cuts at: 159 159
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 522 805 1490 522
Size: 283 685 2790
Fragments arranged by size:
2790 685 283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 1183 1543 1183
Size: 360 3398
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 3275 3275
Size: 3758
SfcI C'TryA_G
Cuts at: 33 518 801 1486 2479 2670 33
Size: 485 283 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 283 191
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 913 1828 2320 2582 913
Size: 915 492 262 2089
Fragments arranged by size:
2089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3758
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
StuI AGG'CCT
Cuts at: 399 399
Size: 3758
StyI C'CwwG_G
Cuts at: 107 892 1246 107
Size: 785 354 2619
Fragments arranged by size:
2619 785 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 215 405 1341 1474 1933 2142 215
Size: 190 936 133 459 209 1831
Fragments arranged by size:
1831 936 459 209 190 133
TatI w'GTAC_w
Cuts at: 3273 3273
Size: 3758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 261 1555 261
Size: 1294 2464
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1253
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI