Restriction map for
pF1KB7937
(Circular) (Six-base) MAPSORT of: pF1KB7937.seq Check: 4337 from: 1 to: 4241
pF1KB7937 4241 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 538 708 1020 538
Size: 170 312 3759
Fragments arranged by size:
3759 312 170
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 971 1040 2066 2498 3245 3513 580
Size: 391 69 1026 432 747 268 1308
Fragments arranged by size:
1308 1026 747 432 391 268 69
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 512 512
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 723 1546 3113 723
Size: 823 1567 1851
Fragments arranged by size:
1851 1567 823
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 579 1156 1245 3594 3850 579
Size: 577 89 2349 256 970
Fragments arranged by size:
2349 970 577 256 89
AseI AT'TA_AT
Cuts at: 20 173 20
Size: 153 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 619 1257 1671 1919 1954 619
Size: 638 414 248 35 2906
Fragments arranged by size:
2906 638 414 248 35
BanII G_rGCy'C
Cuts at: 1046 1255 2285 1046
Size: 209 1030 3002
Fragments arranged by size:
3002 1030 209
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 980 1004 980
Size: 24 4217
BbvCI CC'TCA_GC
Cuts at: 383 398 641 776 383
Size: 15 243 135 3848
Fragments arranged by size:
3848 243 135 15
BciVI GTATCCnnnnn_n'
Cuts at: 933 2139 2900 4241 933
Size: 1206 761 1341 933
Fragments arranged by size:
1341 1206 933 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BfrBI ATG'CAT
Cuts at: 499 499
Size: 4241
BglII A'GATC_T
Cuts at: 151 1756 3793 151
Size: 1605 2037 599
Fragments arranged by size:
2037 1605 599
BlpI GC'TnA_GC
Cuts at: 377 1353 377
Size: 976 3265
Bme1580I G_kGCm'C
Cuts at: 624 1866 1959 3015 624
Size: 1242 93 1056 1850
Fragments arranged by size:
1850 1242 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 944 1453 1534 1869 4013 944
Size: 509 81 335 2144 1172
Fragments arranged by size:
2144 1172 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 758
Size: 4241
Bpu10I CC'TnA_GC
Cuts at: 383 398 534 641 776 383
Size: 15 136 107 135 3848
Fragments arranged by size:
3848 136 135 107 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1150 1417 2296 2788 3086 791
Size: 359 267 879 492 298 1946
Fragments arranged by size:
1946 879 492 359 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 891 921 891
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 719 1006 719
Size: 287 3954
BseYI C'CCAG_C
Cuts at: 335 413 887 1666 2424 3001 3497 335
Size: 78 474 779 758 577 496 1079
Fragments arranged by size:
1079 779 758 577 496 474 78
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3015 1255
Size: 778 190 792 2481
Fragments arranged by size:
2481 792 778 190
BsmI GAATG_Cn'
Cuts at: 501 501
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 624 1046 1255 1866 1959 2033 2223 2285 3015
Size: 422 209 611 93 74 190 62 730
Cuts at: 3015 624
Size: 1850
Fragments arranged by size:
1850 730 611 422 209 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 538 708 1020 1291 1505 1807 2188 408
Size: 130 170 312 271 214 302 381 2461
Fragments arranged by size:
2461 381 312 302 271 214 170 130
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 544 1618 544
Size: 1074 3167
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 151 1266 1733 1756 2091 2337 3349 3435
Size: 150 1115 467 23 335 246 1012 86
Cuts at: 3435 3447 3793 1
Size: 12 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 150
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 867 1826 2000 2391 2418 3763 867
Size: 959 174 391 27 1345 1345
Fragments arranged by size:
1345 1345 959 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 287 737 1034 1188 2264 2474 287
Size: 450 297 154 1076 210 2054
Fragments arranged by size:
2054 1076 450 297 210 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 758 971 1040 2066 2498 3245 3513 580
Size: 178 213 69 1026 432 747 268 1308
Fragments arranged by size:
1308 1026 747 432 268 213 178 69
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 381 413 931 963 1073 1105 2083 2113 2115
Size: 32 518 32 110 32 978 30 2
Cuts at: 2115 2145 381
Size: 30 2477
Fragments arranged by size:
2477 978 518 110 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2912 3096 2912
Size: 184 4057
MscI TGG'CCA
Cuts at: 869 2002 869
Size: 1133 3108
MslI CAynn'nnrTG
Cuts at: 494 2230 2357 494
Size: 1736 127 2378
Fragments arranged by size:
2378 1736 127
MspA1I CmG'CkG
Cuts at: 526 1352 1666 2026 3039 3284 526
Size: 826 314 360 1013 245 1483
Fragments arranged by size:
1483 1013 826 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 107
Size: 4241
NdeI CA'TA_TG
Cuts at: 491 491
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NsiI A_TGCA'T
Cuts at: 501 501
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PfoI T'CCnGG_A
Cuts at: 735 735
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PshAI GACnn'nnGTC
Cuts at: 760 760
Size: 4241
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 924 956 924
Size: 32 4209
PstI C_TGCA'G
Cuts at: 549 1288 1973 549
Size: 739 685 2817
Fragments arranged by size:
2817 739 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 526 1666 2026 526
Size: 1140 360 2741
Fragments arranged by size:
2741 1140 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 287 1034 2264 2474 287
Size: 747 1230 210 2054
Fragments arranged by size:
2054 1230 747 210
SbfI CC_TGCA'GG
Cuts at: 549 1288 549
Size: 739 3502
ScaI AGT'ACT
Cuts at: 221 3758 221
Size: 3537 704
SfcI C'TryA_G
Cuts at: 33 521 545 1284 1969 2962 3153 33
Size: 488 24 739 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 488 191 24
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 806 1165 1396 2311 2803 3065 806
Size: 359 231 915 492 262 1982
Fragments arranged by size:
1982 915 492 359 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 107 1375 1729 107
Size: 1268 354 2619
Fragments arranged by size:
2619 1268 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1824 1957 2416 2625 1824
Size: 133 459 209 3440
Fragments arranged by size:
3440 459 209 133
TatI w'GTAC_w
Cuts at: 219 696 3756 219
Size: 477 3060 704
Fragments arranged by size:
3060 704 477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PshAI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PflMI PmlI
PpuMI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI