Restriction map for
pF1KB7938
(Circular) (Six-base) MAPSORT of: pF1KB7938.seq Check: 9309 from: 1 to: 3812
pF1KB7938 3812 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 185 850 185
Size: 665 3147
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 284 1637 2069 2816 3084 170
Size: 114 1353 432 747 268 898
Fragments arranged by size:
1353 898 747 432 268 114
AflII C'TTAA_G
Cuts at: 780 780
Size: 3812
AflIII A'CryG_T
Cuts at: 180 1164 2268 3245 180
Size: 984 1104 977 747
Fragments arranged by size:
1104 984 977 747
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AleI CACnn'nnGTG
Cuts at: 151 151
Size: 3812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 490
Size: 32 3780
AlwNI CAG_nnn'CTG
Cuts at: 1117 2684 1117
Size: 1567 2245
ApaLI G'TGCA_C
Cuts at: 2582 2582
Size: 3812
ApoI r'AATT_y
Cuts at: 174 269 516 624 816 3165 3421 174
Size: 95 247 108 192 2349 256 565
Fragments arranged by size:
2349 565 256 247 192 108 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 832 832
Size: 3812
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 828 1242 1490 1525 828
Size: 414 248 35 3115
Fragments arranged by size:
3115 414 248 35
BanII G_rGCy'C
Cuts at: 116 708 826 1856 116
Size: 592 118 1030 2072
Fragments arranged by size:
2072 1030 592 118
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 3812
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 222 1332 222
Size: 1110 2702
BglII A'GATC_T
Cuts at: 723 1327 3364 723
Size: 604 2037 1171
Fragments arranged by size:
2037 1171 604
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 1437 1530 2586 1437
Size: 93 1056 2663
Fragments arranged by size:
2663 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 492
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1867 2359 2657 988
Size: 879 492 298 2143
Fragments arranged by size:
2143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 3812
BsaAI yAC'GTr
Cuts at: 181 232 790 1165 181
Size: 51 558 375 2828
Fragments arranged by size:
2828 558 375 51
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 164 595 1243 1491 2193 3689 164
Size: 431 648 248 702 1496 287
Fragments arranged by size:
1496 702 648 431 287 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 490
Size: 30 3782
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 698 151
Size: 547 3265
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 826 1604 1794 2586 826
Size: 778 190 792 2052
Fragments arranged by size:
2052 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 116 708 826 1437 1530 1604 1794 1856 2586
Size: 592 118 611 93 74 190 62 730
Cuts at: 2586 116
Size: 1342
Fragments arranged by size:
1342 730 611 592 190 118 93 74
62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1076 1378 1759 862
Size: 214 302 381 2915
Fragments arranged by size:
2915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 723 837 1304 1327 1662 1908 2920 3006
Size: 722 114 467 23 335 246 1012 86
Cuts at: 3006 3018 3364 1
Size: 12 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 114
86 23 12
BstZ17I GTA'TAC
Cuts at: 186 186
Size: 3812
BtgI C'CryG_G
Cuts at: 107 107
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 1397 1571 1962 1989 3334 1397
Size: 174 391 27 1345 1875
Fragments arranged by size:
1875 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 675 1835 2045 675
Size: 1160 210 2442
Fragments arranged by size:
2442 1160 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 284 492 1637 2069 2816 3084 170
Size: 114 208 1145 432 747 268 898
Fragments arranged by size:
1145 898 747 432 268 208 114
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 3812
EcoO109I rG'GnC_Cy
Cuts at: 141 696 951 141
Size: 555 255 3002
Fragments arranged by size:
3002 555 255
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 1831 183
Size: 1648 2164
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 1246 1494 2516 3495 1246
Size: 248 1022 979 1563
Fragments arranged by size:
1563 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1654 1684 1686 1716 1654
Size: 30 2 30 3750
Fragments arranged by size:
3750 30 30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 1573 1573
Size: 3812
MslI CAynn'nnrTG
Cuts at: 151 230 562 1801 1928 151
Size: 79 332 1239 127 2035
Fragments arranged by size:
2035 1239 332 127 79
MspA1I CmG'CkG
Cuts at: 923 1237 1597 2610 2855 923
Size: 314 360 1013 245 1880
Fragments arranged by size:
1880 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NcoI C'CATG_G
Cuts at: 107 107
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NspI r_CATG'y
Cuts at: 865 1365 2272 865
Size: 500 907 2405
Fragments arranged by size:
2405 907 500
PasI CC'CwG_GG
Cuts at: 700 700
Size: 3812
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 490 522 2951 2983 490
Size: 32 2429 32 1319
Fragments arranged by size:
2429 1319 32 32
PpuMI rG'GwC_Cy
Cuts at: 141 696 141
Size: 555 3257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 642 674 642
Size: 32 3780
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 1237 1597 1237
Size: 360 3452
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SanDI GG'GwC_CC
Cuts at: 696 696
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 727 855 1540 2533 2724 33
Size: 694 128 685 993 191 1121
Fragments arranged by size:
1121 993 694 685 191 128
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 780 967 1882 2374 2636 780
Size: 187 915 492 262 1956
Fragments arranged by size:
1956 915 492 262 187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StyI C'CwwG_G
Cuts at: 107 946 1300 107
Size: 839 354 2619
Fragments arranged by size:
2619 839 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 1395 1528 1987 2196 148
Size: 1247 133 459 209 1764
Fragments arranged by size:
1764 1247 459 209 133
TatI w'GTAC_w
Cuts at: 3327 3327
Size: 3812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 371 403 3329 3361 371
Size: 32 2926 32 822
Fragments arranged by size:
2926 822 32 32
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 636 843 636
Size: 207 3605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
XmnI GAAnn'nnTTC
Cuts at: 362 479 362
Size: 117 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI