Restriction map for
pF1KB7943
(Circular) (Six-base) MAPSORT of: pF1KB7943.seq Check: 8198 from: 1 to: 4244
pF1KB7943 4244 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 429 429
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 1238 4084 1238
Size: 2846 1398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 2069 2501 3248 3516 209
Size: 1860 432 747 268 937
Fragments arranged by size:
1860 937 747 432 268
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AleI CACnn'nnGTG
Cuts at: 817 1088 817
Size: 271 3973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 712 1062 1075 1549 3116 712
Size: 350 13 474 1567 1840
Fragments arranged by size:
1840 1567 474 350 13
ApaI G_GGCC'C
Cuts at: 847 847
Size: 4244
ApaLI G'TGCA_C
Cuts at: 411 934 3014 411
Size: 523 2080 1641
Fragments arranged by size:
2080 1641 523
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 340 431 670 1264 340
Size: 91 239 594 3320
Fragments arranged by size:
3320 594 239 91
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 1260 1674 1922 1957 1260
Size: 414 248 35 3547
Fragments arranged by size:
3547 414 248 35
BanII G_rGCy'C
Cuts at: 532 739 847 1033 1258 2288 532
Size: 207 108 186 225 1030 2488
Fragments arranged by size:
2488 1030 225 207 186 108
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 373 373
Size: 4244
BbvCI CC'TCA_GC
Cuts at: 524 713 524
Size: 189 4055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 601 635 90
Size: 34 477 34 3699
Fragments arranged by size:
3699 477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 196 842 196
Size: 646 3598
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 248 332 623 874 1356 248
Size: 84 291 251 482 3136
Fragments arranged by size:
3136 482 291 251 84
Bme1580I G_kGCm'C
Cuts at: 415 847 938 1001 1869 1962 3018 415
Size: 432 91 63 868 93 1056 1641
Fragments arranged by size:
1641 1056 868 432 93 91 63
BmgBI CAC'GTC
Cuts at: 598 598
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 548 490
Size: 58 4186
Bpu10I CC'TnA_GC
Cuts at: 524 713 524
Size: 189 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2299 2791 3089 1420
Size: 879 492 298 2575
Fragments arranged by size:
2575 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1106 3948 1106
Size: 2842 1402
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 1010 1675 1923 2625 4121 1010
Size: 665 248 702 1496 1133
Fragments arranged by size:
1496 1133 702 665 248
BsaWI w'CCGG_w
Cuts at: 470 1433 1954 2632 2906 3053 3464 470
Size: 963 521 678 274 147 411 1250
Fragments arranged by size:
1250 963 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 798 828 1195 1225 798
Size: 30 367 30 3817
Fragments arranged by size:
3817 367 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 1094 706
Size: 388 3856
BseYI C'CCAG_C
Cuts at: 191 200 290 572 759 1026 1122 1669 2427
Size: 9 90 282 187 267 96 547 758
Cuts at: 2427 3004 3500 191
Size: 577 496 935
Fragments arranged by size:
935 758 577 547 496 282 267 187
96 90 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 160 457 1021 1100 124
Size: 36 297 564 79 3268
Fragments arranged by size:
3268 564 297 79 36
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 415 938 1258 2036 2226 3018 415
Size: 523 320 778 190 792 1641
Fragments arranged by size:
1641 792 778 523 320 190
Bsp1286I G_dGCh'C
Cuts at: 415 532 739 847 938 1001 1033 1258 1869
Size: 117 207 108 91 63 32 225 611
Cuts at: 1869 1962 2036 2226 2288 3018 415
Size: 93 74 190 62 730 1641
Fragments arranged by size:
1641 730 611 225 207 190 117 108
93 91 74 63 62 32
BspEI T'CCGG_A
Cuts at: 470 3464 470
Size: 2994 1250
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 428 429 1190 1294 1508 1810 2191 182
Size: 246 1 761 104 214 302 381 2235
Fragments arranged by size:
2235 761 381 302 246 214 104 1
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 218 2242 2423 2632 218
Size: 2024 181 209 1830
Fragments arranged by size:
2024 1830 209 181
BsrGI T'GTAC_A
Cuts at: 167 167
Size: 4244
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1621 1075
Size: 546 3698
BstBI TT'CG_AA
Cuts at: 950 2605 950
Size: 1655 2589
BstXI CCAn_nnnn'nTGG
Cuts at: 930 930
Size: 4244
BstYI r'GATC_y
Cuts at: 1 1269 1736 1759 2094 2340 3352 3438 3450
Size: 1268 467 23 335 246 1012 86 12
Cuts at: 3450 3796 1
Size: 346 449
Fragments arranged by size:
1268 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 425 425
Size: 4244
BtgI C'CryG_G
Cuts at: 534 534
Size: 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 136 1033 1038 1487 136
Size: 897 5 449 2893
Fragments arranged by size:
2893 897 449 5
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 404 917 1829 2003 2394 2421 3766 404
Size: 513 912 174 391 27 1345 882
Fragments arranged by size:
1345 912 882 513 391 174 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 727 886 2267 2477 727
Size: 159 1381 210 2494
Fragments arranged by size:
2494 1381 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 892 2452 2762 2908 4054 892
Size: 1560 310 146 1146 1082
Fragments arranged by size:
1560 1146 1082 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 490 548 2069 2501 3248 3516 209
Size: 281 58 1521 432 747 268 937
Fragments arranged by size:
1521 937 747 432 281 268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 424 493 424
Size: 69 4175
EcoO109I rG'GnC_Cy
Cuts at: 345 555 696 717 844 1383 345
Size: 210 141 21 127 539 3206
Fragments arranged by size:
3206 539 210 141 127 21
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 164 255 1678 1926 2948 3927 164
Size: 91 1423 248 1022 979 481
Fragments arranged by size:
1423 1022 979 481 248 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 798 830 2086 2116 2118 2148 798
Size: 32 1256 30 2 30 2894
Fragments arranged by size:
2894 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 1014 1283 1014
Size: 269 3975
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 406 2005 406
Size: 1599 2645
MslI CAynn'nnrTG
Cuts at: 817 1088 2233 2360 817
Size: 271 1145 127 2701
Fragments arranged by size:
2701 1145 271 127
MspA1I CmG'CkG
Cuts at: 576 873 982 1026 1075 1355 1669 2029 3042
Size: 297 109 44 49 280 314 360 1013
Cuts at: 3042 3287 576
Size: 245 1533
Fragments arranged by size:
1533 1013 360 314 297 280 245 109
49 44
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 534 534
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 208 235 1297 1797 2704 208
Size: 27 1062 500 907 1748
Fragments arranged by size:
1748 1062 907 500 27
PasI CC'CwG_GG
Cuts at: 701 739 826 1215 701
Size: 38 87 389 3730
Fragments arranged by size:
3730 389 87 38
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 832 912 832
Size: 80 4164
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 118 150 3383 3415 118
Size: 32 3233 32 947
Fragments arranged by size:
3233 947 32 32
PpuMI rG'GwC_Cy
Cuts at: 696 717 696
Size: 21 4223
PspOMI G'GGCC_C
Cuts at: 843 843
Size: 4244
PspXI vC'TCGA_Gb
Cuts at: 340 340
Size: 4244
PstI C_TGCA'G
Cuts at: 494 893 981 1291 1976 494
Size: 399 88 310 685 2762
Fragments arranged by size:
2762 685 399 310 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 576 1669 2029 576
Size: 1093 360 2791
Fragments arranged by size:
2791 1093 360
RsrII CG'GwC_CG
Cuts at: 684 2439 684
Size: 1755 2489
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 727 2267 2477 727
Size: 1540 210 2494
Fragments arranged by size:
2494 1540 210
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SexAI A'CCwGG_T
Cuts at: 929 929
Size: 4244
SfcI C'TryA_G
Cuts at: 33 490 889 977 1287 1972 2965 3156 33
Size: 457 399 88 310 685 993 191 1121
Fragments arranged by size:
1121 993 685 457 399 310 191 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 842 842
Size: 4244
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 433 1266 433
Size: 833 3411
SmlI C'TyrA_G
Cuts at: 340 1399 2314 2806 3068 340
Size: 1059 915 492 262 1516
Fragments arranged by size:
1516 1059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 208 235 1297 1797 208
Size: 27 1062 500 2655
Fragments arranged by size:
2655 1062 500 27
StuI AGG'CCT
Cuts at: 1130 1130
Size: 4244
StyI C'CwwG_G
Cuts at: 534 782 1378 1732 534
Size: 248 596 354 3046
Fragments arranged by size:
3046 596 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1827 1960 2419 2628 1827
Size: 133 459 209 3443
Fragments arranged by size:
3443 459 209 133
TatI w'GTAC_w
Cuts at: 167 3759 167
Size: 3592 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 928 960 1030 1062 1195 1227 3761
Size: 32 52 32 70 32 133 32 2534
Cuts at: 3761 3793 844
Size: 32 1295
Fragments arranged by size:
2534 1295 133 70 52 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 927 1739 816
Size: 111 812 3321
Fragments arranged by size:
3321 812 111
XhoI C'TCGA_G
Cuts at: 340 340
Size: 4244
XmaI C'CCGG_G
Cuts at: 431 1264 431
Size: 833 3411
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PsrI SacII SanDI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI