Restriction map for
pF1KB7951
(Circular) (Six-base) MAPSORT of: pF1KB7951.seq Check: 7704 from: 1 to: 3878
pF1KB7951 3878 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 827 894 827
Size: 67 3811
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1703 2135 2882 3150 818
Size: 885 432 747 268 1546
Fragments arranged by size:
1546 885 747 432 268
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AleI CACnn'nnGTG
Cuts at: 445 445
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 1183 2750 1183
Size: 1567 2311
ApaI G_GGCC'C
Cuts at: 720 720
Size: 3878
ApaLI G'TGCA_C
Cuts at: 2648 2648
Size: 3878
ApoI r'AATT_y
Cuts at: 882 3231 3487 882
Size: 2349 256 1273
Fragments arranged by size:
2349 1273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 378 898 378
Size: 520 3358
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 271 827 894 1308 1556 1591 271
Size: 556 67 414 248 35 2558
Fragments arranged by size:
2558 556 414 248 67 35
BanII G_rGCy'C
Cuts at: 590 720 784 802 892 1922 590
Size: 130 64 18 90 1030 2546
Fragments arranged by size:
2546 1030 130 90 64 18
BbeI G_GCGC'C
Cuts at: 275 1312 1560 275
Size: 1037 248 2593
Fragments arranged by size:
2593 1037 248
BciVI GTATCCnnnnn_n'
Cuts at: 1776 2537 3878 1776
Size: 761 1341 1776
Fragments arranged by size:
1776 1341 761
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BglII A'GATC_T
Cuts at: 626 1393 3430 626
Size: 767 2037 1074
Fragments arranged by size:
2037 1074 767
BlpI GC'TnA_GC
Cuts at: 282 990 282
Size: 708 3170
Bme1580I G_kGCm'C
Cuts at: 224 720 1503 1596 2652 224
Size: 496 783 93 1056 1450
Fragments arranged by size:
1450 1056 783 496 93
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1171 1506 3650 1090
Size: 81 335 2144 1318
Fragments arranged by size:
2144 1318 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 709 430
Size: 279 3599
Bpu10I CC'TnA_GC
Cuts at: 783 783
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1933 2425 2723 1054
Size: 879 492 298 2209
Fragments arranged by size:
2209 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 1231 1231
Size: 3878
BsaBI GATnn'nnATC
Cuts at: 6 1392 3429 6
Size: 1386 2037 455
Fragments arranged by size:
2037 1386 455
BsaHI Gr'CG_yC
Cuts at: 272 1309 1557 2259 3755 272
Size: 1037 248 702 1496 395
Fragments arranged by size:
1496 1037 702 395 248
BsaWI w'CCGG_w
Cuts at: 539 1067 1588 2266 2540 2687 3098 539
Size: 528 521 678 274 147 411 1319
Fragments arranged by size:
1319 678 528 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 784 814 784
Size: 30 3848
BseYI C'CCAG_C
Cuts at: 306 811 1303 2061 2638 3134 306
Size: 505 492 758 577 496 1050
Fragments arranged by size:
1050 758 577 505 496 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3878
BsiEI CG_ry'CG
Cuts at: 104 304 714 1420 1466 2674 104
Size: 200 410 706 46 1208 1308
Fragments arranged by size:
1308 1208 706 410 200 46
BsiHKAI G_wGCw'C
Cuts at: 442 590 892 1670 1860 2652 442
Size: 148 302 778 190 792 1668
Fragments arranged by size:
1668 792 778 302 190 148
Bsp1286I G_dGCh'C
Cuts at: 224 442 590 720 784 802 892 1503 1596
Size: 218 148 130 64 18 90 611 93
Cuts at: 1596 1670 1860 1922 2652 224
Size: 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 218 190 148 130 93
90 74 64 62 18
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 928 1142 1444 1825 928
Size: 214 302 381 2981
Fragments arranged by size:
2981 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2170 2224 3708 3873 50
Size: 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 1876 2057 2266 1876
Size: 181 209 3488
Fragments arranged by size:
3488 209 181
BssSI C'ACGA_G
Cuts at: 2149 2507 2149
Size: 358 3520
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 1255
Size: 3878
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstYI r'GATC_y
Cuts at: 1 488 626 903 1370 1393 1728 1974 2986
Size: 487 138 277 467 23 335 246 1012
Cuts at: 2986 3072 3084 3430 1
Size: 86 12 346 449
Fragments arranged by size:
1012 487 467 449 346 335 277 246
138 86 23 12
BtgI C'CryG_G
Cuts at: 216 216
Size: 3878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 1121 1121
Size: 3878
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DraIII CAC_nnn'GTG
Cuts at: 824 824
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 704 1463 1637 2028 2055 3400 704
Size: 759 174 391 27 1345 1182
Fragments arranged by size:
1345 1182 759 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 430 771 1901 2111 430
Size: 341 1130 210 2197
Fragments arranged by size:
2197 1130 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 598 2086 2396 2542 3688 439
Size: 159 1488 310 146 1146 629
Fragments arranged by size:
1488 1146 629 310 159 146
EcoICRI GAG'CTC
Cuts at: 588 890 588
Size: 302 3576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 709 818 1703 2135 2882 3150 430
Size: 279 109 885 432 747 268 1158
Fragments arranged by size:
1158 885 747 432 279 268 109
EcoO109I rG'GnC_Cy
Cuts at: 414 717 1017 414
Size: 303 300 3275
Fragments arranged by size:
3275 303 300
EcoRI G'AATT_C
Cuts at: 882 882
Size: 3878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3878
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 275 389 1312 1560 2582 3561 275
Size: 114 923 248 1022 979 592
Fragments arranged by size:
1022 979 923 592 248 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1750 1752 1782 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 30 30 2
HincII GTy'rAC
Cuts at: 324 917 324
Size: 593 3285
KasI G'GCGC_C
Cuts at: 271 1308 1556 271
Size: 1037 248 2593
Fragments arranged by size:
2593 1037 248
KpnI G_GTAC'C
Cuts at: 831 898 831
Size: 67 3811
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2733 2549
Size: 184 3694
MscI TGG'CCA
Cuts at: 706 1639 706
Size: 933 2945
MslI CAynn'nnrTG
Cuts at: 112 445 1867 1994 112
Size: 333 1422 127 1996
Fragments arranged by size:
1996 1422 333 127
MspA1I CmG'CkG
Cuts at: 306 989 1303 1663 2676 2921 306
Size: 683 314 360 1013 245 1263
Fragments arranged by size:
1263 1013 683 360 314 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 3878
NarI GG'CG_CC
Cuts at: 272 1309 1557 272
Size: 1037 248 2593
Fragments arranged by size:
2593 1037 248
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 3878
NspI r_CATG'y
Cuts at: 931 1431 2338 931
Size: 500 907 2471
Fragments arranged by size:
2471 907 500
PasI CC'CwG_GG
Cuts at: 418 418
Size: 3878
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PflMI CCAn_nnn'nTGG
Cuts at: 870 870
Size: 3878
PfoI T'CCnGG_A
Cuts at: 772 772
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 748 780 3017 3049 748
Size: 32 2237 32 1577
Fragments arranged by size:
2237 1577 32 32
PpuMI rG'GwC_Cy
Cuts at: 414 414
Size: 3878
PshAI GACnn'nnGTC
Cuts at: 619 619
Size: 3878
PspOMI G'GGCC_C
Cuts at: 716 716
Size: 3878
PstI C_TGCA'G
Cuts at: 925 1610 925
Size: 685 3193
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 1303 1663 1303
Size: 360 3518
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 590 892 590
Size: 302 3576
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 430 1901 2111 430
Size: 1471 210 2197
Fragments arranged by size:
2197 1471 210
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 3395 3395
Size: 3878
SfcI C'TryA_G
Cuts at: 33 276 921 1606 2599 2790 33
Size: 243 645 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 243 191
SfoI GGC'GCC
Cuts at: 273 1310 1558 273
Size: 1037 248 2593
Fragments arranged by size:
2593 1037 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 380 900 380
Size: 520 3358
SmlI C'TyrA_G
Cuts at: 1033 1948 2440 2702 1033
Size: 915 492 262 2209
Fragments arranged by size:
2209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 931 1431 931
Size: 500 3378
StyI C'CwwG_G
Cuts at: 1012 1366 1012
Size: 354 3524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 318 1461 1594 2053 2262 318
Size: 1143 133 459 209 1934
Fragments arranged by size:
1934 1143 459 209 133
TatI w'GTAC_w
Cuts at: 3393 3393
Size: 3878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 378 898 378
Size: 520 3358
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI