Restriction map for
pF1KB7952
(Circular) (Six-base) MAPSORT of: pF1KB7952.seq Check: 5238 from: 1 to: 4295
pF1KB7952 4295 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 1034 1311 1034
Size: 277 4018
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 963 1047 2120 2552 3299 3567 589
Size: 374 84 1073 432 747 268 1317
Fragments arranged by size:
1317 1073 747 432 374 268 84
AflIII A'CryG_T
Cuts at: 357 634 1647 2751 3728 357
Size: 277 1013 1104 977 924
Fragments arranged by size:
1104 1013 977 924 277
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AhdI GACnn_n'nnGTC
Cuts at: 553 553
Size: 4295
AleI CACnn'nnGTG
Cuts at: 872 872
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 1279 1600 3167 1279
Size: 321 1567 2407
Fragments arranged by size:
2407 1567 321
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 929 1299 3648 3904 929
Size: 370 2349 256 1320
Fragments arranged by size:
2349 1320 370 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 154 1315 154
Size: 1161 3134
BamHI G'GATC_C
Cuts at: 789 1320 789
Size: 531 3764
BanI G'GyrC_C
Cuts at: 573 1034 1311 1725 1973 2008 573
Size: 461 277 414 248 35 2860
Fragments arranged by size:
2860 461 414 277 248 35
BanII G_rGCy'C
Cuts at: 431 509 907 1309 2339 431
Size: 78 398 402 1030 2387
Fragments arranged by size:
2387 1030 402 398 78
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 1053 1053
Size: 4295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 263 297 263
Size: 34 4261
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1280 1407 1280
Size: 127 4168
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3069 1920
Size: 93 1056 3146
Fragments arranged by size:
3146 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1588 1923 4067 1507
Size: 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 914 2662 914
Size: 1748 2547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 415 418 508 517 959 310
Size: 105 3 90 9 442 3646
Fragments arranged by size:
3646 442 105 90 9 3
Bpu10I CC'TnA_GC
Cuts at: 712 906 712
Size: 194 4101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 2842 3140 1471
Size: 879 492 298 2626
Fragments arranged by size:
2626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 286 916 3999 286
Size: 630 3083 582
Fragments arranged by size:
3083 630 582
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 437 611 1720 2478 3055 3551 437
Size: 174 1109 758 577 496 1181
Fragments arranged by size:
1181 1109 758 577 496 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 1155 1146
Size: 9 4286
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 367 431 509 907 1309 2087 2277 3069 367
Size: 64 78 398 402 778 190 792 1593
Fragments arranged by size:
1593 792 778 402 398 190 78 64
Bsp1286I G_dGCh'C
Cuts at: 367 431 509 907 1309 1920 2013 2087 2277
Size: 64 78 398 402 611 93 74 190
Cuts at: 2277 2339 3069 367
Size: 62 730 1593
Fragments arranged by size:
1593 730 611 402 398 190 93 78
74 64 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 1133 1672 1133
Size: 539 3756
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 664 664
Size: 4295
BstYI r'GATC_y
Cuts at: 1 384 492 789 1265 1320 1787 1810 2145
Size: 383 108 297 476 55 467 23 335
Cuts at: 2145 2391 3403 3489 3501 3847 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 476 467 449 383 346 335 297
246 108 86 55 23 12
Bsu36I CC'TnA_GG
Cuts at: 617 617
Size: 4295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 844 1038 1239 1538 844
Size: 194 201 299 3601
Fragments arranged by size:
3601 299 201 194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 167 202 1032 1067 167
Size: 35 830 35 3395
Fragments arranged by size:
3395 830 35 35
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 1275 1275
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 606 2001 2859 606
Size: 1395 858 2042
Fragments arranged by size:
2042 1395 858
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 464 883 2318 2528 464
Size: 419 1435 210 2231
Fragments arranged by size:
2231 1435 419 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 429 507 905 1307 429
Size: 78 398 402 3417
Fragments arranged by size:
3417 402 398 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 415 418 508 517 589 959 963 1047
Size: 105 3 90 9 72 370 4 84
Cuts at: 1047 2120 2552 3299 3567 310
Size: 1073 432 747 268 1038
Fragments arranged by size:
1073 1038 747 432 370 268 105 90
84 72 9 4 3
EcoO109I rG'GnC_Cy
Cuts at: 1434 1434
Size: 4295
EcoRI G'AATT_C
Cuts at: 929 1299 929
Size: 370 3925
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 1729 1977 2999 3978 1729
Size: 248 1022 979 2046
Fragments arranged by size:
2046 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 369 401 539 561 571 593 894
Size: 32 219 32 138 22 10 22 301
Cuts at: 894 926 1062 1094 1134 1166 2137 2167 2169
Size: 32 136 32 40 32 971 30 2
Cuts at: 2169 2199 118
Size: 30 2214
Fragments arranged by size:
2214 971 301 219 138 136 40 32
32 32 32 32 30 30 22 22
10 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
HindIII A'AGCT_T
Cuts at: 1227 1227
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1038 1315 1038
Size: 277 4018
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 872 2284 2411 872
Size: 1412 127 2756
Fragments arranged by size:
2756 1412 127
MspA1I CmG'CkG
Cuts at: 279 1406 1720 2080 3093 3338 279
Size: 1127 314 360 1013 245 1236
Fragments arranged by size:
1236 1127 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 361 638 1348 1848 2755 361
Size: 277 710 500 907 1901
Fragments arranged by size:
1901 907 710 500 277
PasI CC'CwG_GG
Cuts at: 138 138
Size: 4295
PciI A'CATG_T
Cuts at: 357 634 2751 357
Size: 277 2117 1901
Fragments arranged by size:
2117 1901 277
PflMI CCAn_nnn'nTGG
Cuts at: 463 1275 463
Size: 812 3483
PfoI T'CCnGG_A
Cuts at: 326 326
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PstI C_TGCA'G
Cuts at: 1342 2027 1342
Size: 685 3610
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 279 1720 2080 279
Size: 1441 360 2494
Fragments arranged by size:
2494 1441 360
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 431 509 907 1309 431
Size: 78 398 402 3417
Fragments arranged by size:
3417 402 398 78
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 883 2318 2528 883
Size: 1435 210 2650
Fragments arranged by size:
2650 1435 210
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 1146 1338 2023 3016 3207 33
Size: 1113 192 685 993 191 1121
Fragments arranged by size:
1121 1113 993 685 192 191
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 1450 2365 2857 3119 1450
Size: 915 492 262 2626
Fragments arranged by size:
2626 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StyI C'CwwG_G
Cuts at: 304 422 1429 1783 304
Size: 118 1007 354 2816
Fragments arranged by size:
2816 1007 354 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 429 1878 2011 2470 2679 130
Size: 299 1449 133 459 209 1746
Fragments arranged by size:
1746 1449 459 299 209 133
TatI w'GTAC_w
Cuts at: 3810 3810
Size: 4295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 1790 295
Size: 1495 2800
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 170 976 1060 170
Size: 806 84 3405
Fragments arranged by size:
3405 806 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI