Restriction map for
pF1KB7953
(Circular) (Six-base) MAPSORT of: pF1KB7953.seq Check: 1816 from: 1 to: 3881
pF1KB7953 3881 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 2138 2885 3153 1706
Size: 432 747 268 2434
Fragments arranged by size:
2434 747 432 268
AflIII A'CryG_T
Cuts at: 1233 2337 3314 1233
Size: 1104 977 1800
Fragments arranged by size:
1800 1104 977
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AlwNI CAG_nnn'CTG
Cuts at: 1186 2753 1186
Size: 1567 2314
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 885 3234 3490 885
Size: 2349 256 1276
Fragments arranged by size:
2349 1276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 163 901 163
Size: 738 3143
BamHI G'GATC_C
Cuts at: 257 906 257
Size: 649 3232
BanI G'GyrC_C
Cuts at: 897 1311 1559 1594 897
Size: 414 248 35 3184
Fragments arranged by size:
3184 414 248 35
BanII G_rGCy'C
Cuts at: 286 850 895 1925 286
Size: 564 45 1030 2242
Fragments arranged by size:
2242 1030 564 45
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BbsI GAAGACnn'nnnn_
Cuts at: 446 446
Size: 3881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3847
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 1401 1401
Size: 3881
BglI GCCn_nnn'nGGC
Cuts at: 339 339
Size: 3881
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 217 993 217
Size: 776 3105
Bme1580I G_kGCm'C
Cuts at: 170 592 1506 1599 2655 170
Size: 422 914 93 1056 1396
Fragments arranged by size:
1396 1056 914 422 93
BmrI ACTGGGnnnn_n'
Cuts at: 1093 1174 1509 3653 1093
Size: 81 335 2144 1321
Fragments arranged by size:
2144 1321 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 835 328
Size: 507 3374
Bpu10I CC'TnA_GC
Cuts at: 119 119
Size: 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1057 1936 2428 2726 592
Size: 465 879 492 298 1747
Fragments arranged by size:
1747 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 3881
BsaAI yAC'GTr
Cuts at: 750 1234 750
Size: 484 3397
BsaBI GATnn'nnATC
Cuts at: 6 1395 3432 6
Size: 1389 2037 455
Fragments arranged by size:
2037 1389 455
BsaHI Gr'CG_yC
Cuts at: 136 1312 1560 2262 3758 136
Size: 1176 248 702 1496 259
Fragments arranged by size:
1496 1176 702 259 248
BsaWI w'CCGG_w
Cuts at: 776 1070 1591 2269 2543 2690 3101 776
Size: 294 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 3851
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 676
Size: 3881
BseYI C'CCAG_C
Cuts at: 1306 2064 2641 3137 1306
Size: 758 577 496 2050
Fragments arranged by size:
2050 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1423 1469 2677 104
Size: 1319 46 1208 1308
Fragments arranged by size:
1319 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 749 850 895 1673 1863 2655 749
Size: 101 45 778 190 792 1975
Fragments arranged by size:
1975 792 778 190 101 45
Bsp1286I G_dGCh'C
Cuts at: 170 286 592 749 850 895 1506 1599 1673
Size: 116 306 157 101 45 611 93 74
Cuts at: 1673 1863 1925 2655 170
Size: 190 62 730 1396
Fragments arranged by size:
1396 730 611 306 190 157 116 101
93 74 62 45
BspEI T'CCGG_A
Cuts at: 3101 3101
Size: 3881
BspHI T'CATG_A
Cuts at: 677 842 3057 677
Size: 165 2215 1501
Fragments arranged by size:
2215 1501 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 931 1145 1447 1828 931
Size: 214 302 381 2984
Fragments arranged by size:
2984 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2173 2227 3711 3876 50
Size: 2123 54 1484 165 55
Fragments arranged by size:
2123 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 1793
Size: 3881
BsrFI r'CCGG_y
Cuts at: 114 738 1879 2060 2269 114
Size: 624 1141 181 209 1726
Fragments arranged by size:
1726 1141 624 209 181
BssSI C'ACGA_G
Cuts at: 598 2152 2510 598
Size: 1554 358 1969
Fragments arranged by size:
1969 1554 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1258 1258
Size: 3881
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstXI CCAn_nnnn'nTGG
Cuts at: 429 429
Size: 3881
BstYI r'GATC_y
Cuts at: 1 257 906 1373 1396 1731 1977 2989 3075
Size: 256 649 467 23 335 246 1012 86
Cuts at: 3075 3087 3433 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 256 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 476 476
Size: 3881
BtgI C'CryG_G
Cuts at: 317 422 317
Size: 105 3776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1831 2012 2175 1831
Size: 181 163 3537
Fragments arranged by size:
3537 181 163
BtsI GCAGTG_nn'
Cuts at: 1124 1124
Size: 3881
DraI TTT'AAA
Cuts at: 880 880
Size: 3881
DrdI GACnn_nn'nnGTC
Cuts at: 135 1587 2445 135
Size: 1452 858 1571
Fragments arranged by size:
1571 1452 858
EaeI y'GGCC_r
Cuts at: 671 1466 1640 2031 2058 3403 671
Size: 795 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 795 391 174 27
EagI C'GGCC_G
Cuts at: 1466 1466
Size: 3881
EarI CTCTTCn'nnn_
Cuts at: 514 800 1904 2114 514
Size: 286 1104 210 2281
Fragments arranged by size:
2281 1104 286 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 757 2089 2399 2545 3691 757
Size: 1332 310 146 1146 947
Fragments arranged by size:
1332 1146 947 310 146
EcoICRI GAG'CTC
Cuts at: 848 893 848
Size: 45 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 835 1706 2138 2885 3153 328
Size: 507 871 432 747 268 1056
Fragments arranged by size:
1056 871 747 507 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 727 727
Size: 3881
EcoO109I rG'GnC_Cy
Cuts at: 159 1020 159
Size: 861 3020
EcoRI G'AATT_C
Cuts at: 885 885
Size: 3881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 1900 633
Size: 1267 2614
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 1315 1563 2585 3564 1315
Size: 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1723 1753 1755 1785 373
Size: 32 1318 30 2 30 2469
Fragments arranged by size:
2469 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 920 920
Size: 3881
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 2552 2736 119
Size: 2433 184 1264
Fragments arranged by size:
2433 1264 184
MscI TGG'CCA
Cuts at: 673 1642 673
Size: 969 2912
MslI CAynn'nnrTG
Cuts at: 1870 1997 1870
Size: 127 3754
MspA1I CmG'CkG
Cuts at: 351 858 992 1306 1666 2679 2924 351
Size: 507 134 314 360 1013 245 1308
Fragments arranged by size:
1308 1013 507 360 314 245 134
NaeI GCC'GGC
Cuts at: 116 740 2062 116
Size: 624 1322 1935
Fragments arranged by size:
1935 1322 624
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NgoMIV G'CCGG_C
Cuts at: 114 738 2060 114
Size: 624 1322 1935
Fragments arranged by size:
1935 1322 624
NspI r_CATG'y
Cuts at: 934 1434 2341 934
Size: 500 907 2474
Fragments arranged by size:
2474 907 500
PciI A'CATG_T
Cuts at: 2337 2337
Size: 3881
PfoI T'CCnGG_A
Cuts at: 812 812
Size: 3881
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PmlI CAC'GTG
Cuts at: 750 750
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 3881
PstI C_TGCA'G
Cuts at: 356 389 928 1613 356
Size: 33 539 685 2624
Fragments arranged by size:
2624 685 539 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 351 858 1306 1666 351
Size: 507 448 360 2566
Fragments arranged by size:
2566 507 448 360
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 850 895 850
Size: 45 3836
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
SbfI CC_TGCA'GG
Cuts at: 928 928
Size: 3881
ScaI AGT'ACT
Cuts at: 3398 3398
Size: 3881
SfcI C'TryA_G
Cuts at: 33 352 385 396 924 1609 2602 2793 33
Size: 319 33 11 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 319 191 33 11
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SgrAI Cr'CCGG_yG
Cuts at: 738 738
Size: 3881
SmaI CCC'GGG
Cuts at: 903 903
Size: 3881
SmlI C'TyrA_G
Cuts at: 607 1036 1951 2443 2705 607
Size: 429 915 492 262 1783
Fragments arranged by size:
1783 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 934 1434 934
Size: 500 3381
StuI AGG'CCT
Cuts at: 820 820
Size: 3881
StyI C'CwwG_G
Cuts at: 1015 1369 1015
Size: 354 3527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1464 1597 2056 2265 1464
Size: 133 459 209 3080
Fragments arranged by size:
3080 459 209 133
TatI w'GTAC_w
Cuts at: 3396 3396
Size: 3881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 3849
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 912 912
Size: 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 1376 429
Size: 947 2934
XmaI C'CCGG_G
Cuts at: 901 901
Size: 3881
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI