Restriction map for
pF1KB7957
(Circular) (Six-base) MAPSORT of: pF1KB7957.seq Check: 6347 from: 1 to: 3896
pF1KB7957 3896 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 232 232
Size: 3896
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 809 1721 2153 2900 3168 347
Size: 462 912 432 747 268 1075
Fragments arranged by size:
1075 912 747 462 432 268
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AleI CACnn'nnGTG
Cuts at: 217 217
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 621 1201 2768 621
Size: 580 1567 1749
Fragments arranged by size:
1749 1567 580
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 271 916 271
Size: 645 3251
BamHI G'GATC_C
Cuts at: 554 921 554
Size: 367 3529
BanI G'GyrC_C
Cuts at: 912 1326 1574 1609 912
Size: 414 248 35 3199
Fragments arranged by size:
3199 414 248 35
BanII G_rGCy'C
Cuts at: 677 910 1940 677
Size: 233 1030 2633
Fragments arranged by size:
2633 1030 233
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbvCI CC'TCA_GC
Cuts at: 514 841 514
Size: 327 3569
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 891 2263 891
Size: 1372 2524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 598 470
Size: 128 3768
Bpu10I CC'TnA_GC
Cuts at: 403 514 841 403
Size: 111 327 3458
Fragments arranged by size:
3458 327 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 652 1072 1951 2443 2741 174
Size: 478 420 879 492 298 1329
Fragments arranged by size:
1329 879 492 478 420 298
BsaI GGTCTCn'nnnn_
Cuts at: 832 3600 832
Size: 2768 1128
BsaAI yAC'GTr
Cuts at: 366 1249 366
Size: 883 3013
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 536 1085 1606 2284 2558 2705 3116 536
Size: 549 521 678 274 147 411 1316
Fragments arranged by size:
1316 678 549 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 820 820
Size: 3896
BseYI C'CCAG_C
Cuts at: 121 355 1321 2079 2656 3152 121
Size: 234 966 758 577 496 865
Fragments arranged by size:
966 865 758 577 496 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 254 1438 1484 2692 104
Size: 150 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 150 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 297 297
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 677 910 1521 1614 1688 1878 1940 2670 677
Size: 233 611 93 74 190 62 730 1903
Fragments arranged by size:
1903 730 611 233 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 232 946 1160 1462 1843 232
Size: 714 214 302 381 2285
Fragments arranged by size:
2285 714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 702 2188 2242 3726 3891 50
Size: 652 1486 54 1484 165 55
Fragments arranged by size:
1486 1484 652 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BsrGI T'GTAC_A
Cuts at: 581 581
Size: 3896
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 219 755 219
Size: 536 3360
BstYI r'GATC_y
Cuts at: 1 554 921 1388 1411 1746 1992 3004 3090
Size: 553 367 467 23 335 246 1012 86
Cuts at: 3090 3102 3448 1
Size: 12 346 449
Fragments arranged by size:
1012 553 467 449 367 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 188 218 381 107
Size: 81 30 163 3622
Fragments arranged by size:
3622 163 81 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 558 1139 558
Size: 581 3315
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 321 871 1602 2460 321
Size: 550 731 858 1757
Fragments arranged by size:
1757 858 731 550
EaeI y'GGCC_r
Cuts at: 251 368 1481 1655 2046 2073 3418 251
Size: 117 1113 174 391 27 1345 729
Fragments arranged by size:
1345 1113 729 391 174 117 27
EagI C'GGCC_G
Cuts at: 251 1481 251
Size: 1230 2666
EarI CTCTTCn'nnn_
Cuts at: 818 1919 2129 818
Size: 1101 210 2585
Fragments arranged by size:
2585 1101 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 499 661 2104 2414 2560 3706 499
Size: 162 1443 310 146 1146 689
Fragments arranged by size:
1443 1146 689 310 162 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 470 598 809 1721 2153 2900 3168 347
Size: 123 128 211 912 432 747 268 1075
Fragments arranged by size:
1075 912 747 432 268 211 128 123
EcoNI CCTnn'n_nnAGG
Cuts at: 667 667
Size: 3896
EcoO109I rG'GnC_Cy
Cuts at: 178 399 455 618 1035 178
Size: 221 56 163 417 3039
Fragments arranged by size:
3039 417 221 163 56
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 1738 1768 1770 1800 619
Size: 32 1087 30 2 30 2715
Fragments arranged by size:
2715 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
HindIII A'AGCT_T
Cuts at: 150 150
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 370 1657 370
Size: 1287 2609
MslI CAynn'nnrTG
Cuts at: 217 1885 2012 217
Size: 1668 127 2101
Fragments arranged by size:
2101 1668 127
MspA1I CmG'CkG
Cuts at: 1007 1321 1681 2694 2939 1007
Size: 314 360 1013 245 1964
Fragments arranged by size:
1964 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 188 381 107
Size: 81 193 3622
Fragments arranged by size:
3622 193 81
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PasI CC'CwG_GG
Cuts at: 225 225
Size: 3896
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PflMI CCAn_nnn'nTGG
Cuts at: 621 621
Size: 3896
PfoI T'CCnGG_A
Cuts at: 467 830 467
Size: 363 3533
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PmlI CAC'GTG
Cuts at: 366 366
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 178 178
Size: 3896
PshAI GACnn'nnGTC
Cuts at: 289 289
Size: 3896
PstI C_TGCA'G
Cuts at: 689 943 1628 689
Size: 254 685 2957
Fragments arranged by size:
2957 685 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 549 685 939 1624 2617 2808 33
Size: 516 136 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 254 191 136
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 153 667 1051 1966 2458 2720 153
Size: 514 384 915 492 262 1329
Fragments arranged by size:
1329 915 514 492 384 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StyI C'CwwG_G
Cuts at: 107 168 188 381 1030 1384 107
Size: 61 20 193 649 354 2619
Fragments arranged by size:
2619 649 354 193 61 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 374 1479 1612 2071 2280 374
Size: 1105 133 459 209 1990
Fragments arranged by size:
1990 1105 459 209 133
TatI w'GTAC_w
Cuts at: 581 3411 581
Size: 2830 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 1391 363
Size: 1028 2868
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI