(Circular) (Six-base) MAPSORT of: pF1KB7965.seq Check: 4696 from: 1 to: 3953 pF1KB7965 3953 bp With 182 enzymes: SgfI * October 7, 2009 14:25 .. AccI GT'mk_AC Cuts at: 848 991 848 Size: 143 3810 Acc65I G'GTAC_C Cuts at: 771 969 771 Size: 198 3755 AclI AA'CG_TT Cuts at: 947 3793 947 Size: 2846 1107 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 322 432 1778 2210 2957 3225 322 Size: 110 1346 432 747 268 1050 Fragments arranged by size: 1346 1050 747 432 268 110 AflIII A'CryG_T Cuts at: 1305 2409 3386 1305 Size: 1104 977 1872 Fragments arranged by size: 1872 1104 977 AgeI A'CCGG_T Cuts at: 2341 2341 Size: 3953 AleI CACnn'nnGTG Cuts at: 871 871 Size: 3953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 538 538 Size: 3953 AlwNI CAG_nnn'CTG Cuts at: 202 1258 2825 202 Size: 1056 1567 1330 Fragments arranged by size: 1567 1330 1056 ApaI G_GGCC'C Cuts at: 880 880 Size: 3953 ApaLI G'TGCA_C Cuts at: 2723 2723 Size: 3953 ApoI r'AATT_y Cuts at: 132 957 3306 3562 132 Size: 825 2349 256 523 Fragments arranged by size: 2349 825 523 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3953 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3953 AvaI C'yCGr_G Cuts at: 574 679 973 574 Size: 105 294 3554 Fragments arranged by size: 3554 294 105 BamHI G'GATC_C Cuts at: 978 978 Size: 3953 BanI G'GyrC_C Cuts at: 600 690 706 771 865 969 1383 1631 1666 Size: 90 16 65 94 104 414 248 35 Cuts at: 1666 600 Size: 2887 Fragments arranged by size: 2887 414 248 104 94 90 65 35 16 BanII G_rGCy'C Cuts at: 880 967 1997 880 Size: 87 1030 2836 Fragments arranged by size: 2836 1030 87 BbeI G_GCGC'C Cuts at: 604 694 710 1387 1635 604 Size: 90 16 677 248 2922 Fragments arranged by size: 2922 677 248 90 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 3919 BciVI GTATCCnnnnn_n' Cuts at: 1851 2612 3953 1851 Size: 761 1341 1851 Fragments arranged by size: 1851 1341 761 BclI T'GATC_A Cuts at: 1473 1473 Size: 3953 BglI GCCn_nnn'nGGC Cuts at: 598 598 Size: 3953 BglII A'GATC_T Cuts at: 1468 3505 1468 Size: 2037 1916 BlpI GC'TnA_GC Cuts at: 406 1065 406 Size: 659 3294 Bme1580I G_kGCm'C Cuts at: 674 880 1578 1671 2727 674 Size: 206 698 93 1056 1900 Fragments arranged by size: 1900 1056 698 206 93 BmrI ACTGGGnnnn_n' Cuts at: 1165 1246 1581 3725 1165 Size: 81 335 2144 1393 Fragments arranged by size: 2144 1393 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 3953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 352 755 352 Size: 403 3550 Bpu10I CC'TnA_GC Cuts at: 746 746 Size: 3953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1129 2008 2500 2798 1129 Size: 879 492 298 2284 Fragments arranged by size: 2284 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3657 3657 Size: 3953 BsaAI yAC'GTr Cuts at: 488 1306 488 Size: 818 3135 BsaBI GATnn'nnATC Cuts at: 6 1467 3504 6 Size: 1461 2037 455 Fragments arranged by size: 2037 1461 455 BsaHI Gr'CG_yC Cuts at: 601 691 707 1384 1632 2334 3830 601 Size: 90 16 677 248 702 1496 724 Fragments arranged by size: 1496 724 702 677 248 90 16 BsaWI w'CCGG_w Cuts at: 180 371 1142 1663 2341 2615 2762 3173 180 Size: 191 771 521 678 274 147 411 960 Fragments arranged by size: 960 771 678 521 411 274 191 147 BseYI C'CCAG_C Cuts at: 159 426 635 720 1378 2136 2713 3209 159 Size: 267 209 85 658 758 577 496 903 Fragments arranged by size: 903 758 658 577 496 267 209 85 BsiEI CG_ry'CG Cuts at: 104 1495 1541 2749 104 Size: 1391 46 1208 1308 Fragments arranged by size: 1391 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 209 412 967 1745 1935 2727 209 Size: 203 555 778 190 792 1435 Fragments arranged by size: 1435 792 778 555 203 190 BsmI GAATG_Cn' Cuts at: 321 321 Size: 3953 Bsp1286I G_dGCh'C Cuts at: 209 412 674 880 967 1578 1671 1745 1935 Size: 203 262 206 87 611 93 74 190 Cuts at: 1935 1997 2727 209 Size: 62 730 1435 Fragments arranged by size: 1435 730 611 262 206 203 190 93 87 74 62 BspEI T'CCGG_A Cuts at: 371 3173 371 Size: 2802 1151 BspHI T'CATG_A Cuts at: 3129 3129 Size: 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 386 1003 1217 1519 1900 386 Size: 617 214 302 381 2439 Fragments arranged by size: 2439 617 381 302 214 BsrBI CCG'CTC Cuts at: 50 2245 2299 3783 3948 50 Size: 2195 54 1484 165 55 Fragments arranged by size: 2195 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1865 1865 Size: 3953 BsrFI r'CCGG_y Cuts at: 442 774 1951 2132 2341 442 Size: 332 1177 181 209 2054 Fragments arranged by size: 2054 1177 332 209 181 BssSI C'ACGA_G Cuts at: 667 2224 2582 667 Size: 1557 358 2038 Fragments arranged by size: 2038 1557 358 BstAPI GCAn_nnn'nTGC Cuts at: 1330 1330 Size: 3953 BstBI TT'CG_AA Cuts at: 2314 2314 Size: 3953 BstEII G'GTnAC_C Cuts at: 468 468 Size: 3953 BstYI r'GATC_y Cuts at: 1 978 1445 1468 1803 2049 3061 3147 3159 Size: 977 467 23 335 246 1012 86 12 Cuts at: 3159 3505 1 Size: 346 449 Fragments arranged by size: 1012 977 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 872 937 872 Size: 65 3888 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1903 2084 2247 1903 Size: 181 163 3609 Fragments arranged by size: 3609 181 163 BtsI GCAGTG_nn' Cuts at: 893 1196 893 Size: 303 3650 DraI TTT'AAA Cuts at: 952 952 Size: 3953 DrdI GACnn_nn'nnGTC Cuts at: 1659 2517 1659 Size: 858 3095 EaeI y'GGCC_r Cuts at: 528 536 786 1538 1712 2103 2130 3475 528 Size: 8 250 752 174 391 27 1345 1006 Fragments arranged by size: 1345 1006 752 391 250 174 27 8 EagI C'GGCC_G Cuts at: 1538 1538 Size: 3953 EarI CTCTTCn'nnn_ Cuts at: 240 1976 2186 240 Size: 1736 210 2007 Fragments arranged by size: 2007 1736 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 355 934 957 2161 2471 2617 3763 355 Size: 579 23 1204 310 146 1146 545 Fragments arranged by size: 1204 1146 579 545 310 146 23 EcoICRI GAG'CTC Cuts at: 965 965 Size: 3953 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 322 352 432 755 1778 2210 2957 3225 322 Size: 30 80 323 1023 432 747 268 1050 Fragments arranged by size: 1050 1023 747 432 323 268 80 30 EcoO109I rG'GnC_Cy Cuts at: 622 815 877 1092 622 Size: 193 62 215 3483 Fragments arranged by size: 3483 215 193 62 EcoRI G'AATT_C Cuts at: 957 957 Size: 3953 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1972 1972 Size: 3953 FspI TGC'GCA Cuts at: 514 1734 514 Size: 1220 2733 FspAI rTGC'GCAy Cuts at: 514 514 Size: 3953 HaeII r_GCGC'y Cuts at: 604 694 710 1387 1635 2657 3636 604 Size: 90 16 677 248 1022 979 921 Fragments arranged by size: 1022 979 921 677 248 90 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1795 1825 1827 1857 1795 Size: 30 2 30 3891 Fragments arranged by size: 3891 30 30 2 HincII GTy'rAC Cuts at: 171 992 171 Size: 821 3132 KasI G'GCGC_C Cuts at: 600 690 706 1383 1631 600 Size: 90 16 677 248 2922 Fragments arranged by size: 2922 677 248 90 16 KpnI G_GTAC'C Cuts at: 775 973 775 Size: 198 3755 MluI A'CGCG_T Cuts at: 3386 3386 Size: 3953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2624 2808 2624 Size: 184 3769 MscI TGG'CCA Cuts at: 538 788 1714 538 Size: 250 926 2777 Fragments arranged by size: 2777 926 250 MslI CAynn'nnrTG Cuts at: 871 1942 2069 871 Size: 1071 127 2755 Fragments arranged by size: 2755 1071 127 MspA1I CmG'CkG Cuts at: 115 276 384 830 863 902 1064 1378 1738 Size: 161 108 446 33 39 162 314 360 Cuts at: 1738 2751 2996 115 Size: 1013 245 1072 Fragments arranged by size: 1072 1013 446 360 314 245 162 161 108 39 33 NaeI GCC'GGC Cuts at: 444 2134 444 Size: 1690 2263 NarI GG'CG_CC Cuts at: 601 691 707 1384 1632 601 Size: 90 16 677 248 2922 Fragments arranged by size: 2922 677 248 90 16 NgoMIV G'CCGG_C Cuts at: 442 2132 442 Size: 1690 2263 NspI r_CATG'y Cuts at: 1006 1506 2413 1006 Size: 500 907 2546 Fragments arranged by size: 2546 907 500 PciI A'CATG_T Cuts at: 2409 2409 Size: 3953 PmeI GTTT'AAAC Cuts at: 952 952 Size: 3953 PmlI CAC'GTG Cuts at: 488 488 Size: 3953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3092 3124 3092 Size: 32 3921 PpuMI rG'GwC_Cy Cuts at: 622 622 Size: 3953 PspOMI G'GGCC_C Cuts at: 876 876 Size: 3953 PstI C_TGCA'G Cuts at: 383 1000 1685 383 Size: 617 685 2651 Fragments arranged by size: 2651 685 617 PvuI CG_AT'CG Cuts at: 104 104 Size: 3953 PvuII CAG'CTG Cuts at: 115 276 1378 1738 115 Size: 161 1102 360 2330 Fragments arranged by size: 2330 1102 360 161 RsrII CG'GwC_CG Cuts at: 2148 2148 Size: 3953 SacI G_AGCT'C Cuts at: 967 967 Size: 3953 SalI G'TCGA_C Cuts at: 990 990 Size: 3953 SanDI GG'GwC_CC Cuts at: 622 622 Size: 3953 SapI GCTCTTCn'nnn_ Cuts at: 240 1976 2186 240 Size: 1736 210 2007 Fragments arranged by size: 2007 1736 210 SbfI CC_TGCA'GG Cuts at: 1000 1000 Size: 3953 ScaI AGT'ACT Cuts at: 3470 3470 Size: 3953 SfcI C'TryA_G Cuts at: 33 379 996 1681 2674 2865 33 Size: 346 617 685 993 191 1121 Fragments arranged by size: 1121 993 685 617 346 191 SfoI GGC'GCC Cuts at: 602 692 708 1385 1633 602 Size: 90 16 677 248 2922 Fragments arranged by size: 2922 677 248 90 16 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3953 SmaI CCC'GGG Cuts at: 576 681 975 576 Size: 105 294 3554 Fragments arranged by size: 3554 294 105 SmlI C'TyrA_G Cuts at: 1108 2023 2515 2777 1108 Size: 915 492 262 2284 Fragments arranged by size: 2284 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3953 SphI G_CATG'C Cuts at: 1006 1506 1006 Size: 500 3453 StyI C'CwwG_G Cuts at: 1087 1441 1087 Size: 354 3599 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1536 1669 2128 2337 1536 Size: 133 459 209 3152 Fragments arranged by size: 3152 459 209 133 TatI w'GTAC_w Cuts at: 3468 3468 Size: 3953 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 913 945 3470 3502 913 Size: 32 2525 32 1364 Fragments arranged by size: 2525 1364 32 32 Tth111I GACn'n_nGTC Cuts at: 1750 1750 Size: 3953 XbaI T'CTAG_A Cuts at: 984 984 Size: 3953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1448 1448 Size: 3953 XmaI C'CCGG_G Cuts at: 574 679 973 574 Size: 105 294 3554 Fragments arranged by size: 3554 294 105 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI