Restriction map for
pF1KB7965
(Circular) (Six-base) MAPSORT of: pF1KB7965.seq Check: 4696 from: 1 to: 3953
pF1KB7965 3953 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 848 991 848
Size: 143 3810
Acc65I G'GTAC_C
Cuts at: 771 969 771
Size: 198 3755
AclI AA'CG_TT
Cuts at: 947 3793 947
Size: 2846 1107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 432 1778 2210 2957 3225 322
Size: 110 1346 432 747 268 1050
Fragments arranged by size:
1346 1050 747 432 268 110
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AleI CACnn'nnGTG
Cuts at: 871 871
Size: 3953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 202 1258 2825 202
Size: 1056 1567 1330
Fragments arranged by size:
1567 1330 1056
ApaI G_GGCC'C
Cuts at: 880 880
Size: 3953
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 132 957 3306 3562 132
Size: 825 2349 256 523
Fragments arranged by size:
2349 825 523 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 574 679 973 574
Size: 105 294 3554
Fragments arranged by size:
3554 294 105
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 600 690 706 771 865 969 1383 1631 1666
Size: 90 16 65 94 104 414 248 35
Cuts at: 1666 600
Size: 2887
Fragments arranged by size:
2887 414 248 104 94 90 65 35
16
BanII G_rGCy'C
Cuts at: 880 967 1997 880
Size: 87 1030 2836
Fragments arranged by size:
2836 1030 87
BbeI G_GCGC'C
Cuts at: 604 694 710 1387 1635 604
Size: 90 16 677 248 2922
Fragments arranged by size:
2922 677 248 90 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3919
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglI GCCn_nnn'nGGC
Cuts at: 598 598
Size: 3953
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 406 1065 406
Size: 659 3294
Bme1580I G_kGCm'C
Cuts at: 674 880 1578 1671 2727 674
Size: 206 698 93 1056 1900
Fragments arranged by size:
1900 1056 698 206 93
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 755 352
Size: 403 3550
Bpu10I CC'TnA_GC
Cuts at: 746 746
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3657 3657
Size: 3953
BsaAI yAC'GTr
Cuts at: 488 1306 488
Size: 818 3135
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 601 691 707 1384 1632 2334 3830 601
Size: 90 16 677 248 702 1496 724
Fragments arranged by size:
1496 724 702 677 248 90 16
BsaWI w'CCGG_w
Cuts at: 180 371 1142 1663 2341 2615 2762 3173 180
Size: 191 771 521 678 274 147 411 960
Fragments arranged by size:
960 771 678 521 411 274 191 147
BseYI C'CCAG_C
Cuts at: 159 426 635 720 1378 2136 2713 3209 159
Size: 267 209 85 658 758 577 496 903
Fragments arranged by size:
903 758 658 577 496 267 209 85
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 209 412 967 1745 1935 2727 209
Size: 203 555 778 190 792 1435
Fragments arranged by size:
1435 792 778 555 203 190
BsmI GAATG_Cn'
Cuts at: 321 321
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 209 412 674 880 967 1578 1671 1745 1935
Size: 203 262 206 87 611 93 74 190
Cuts at: 1935 1997 2727 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 611 262 206 203 190 93
87 74 62
BspEI T'CCGG_A
Cuts at: 371 3173 371
Size: 2802 1151
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 1003 1217 1519 1900 386
Size: 617 214 302 381 2439
Fragments arranged by size:
2439 617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 442 774 1951 2132 2341 442
Size: 332 1177 181 209 2054
Fragments arranged by size:
2054 1177 332 209 181
BssSI C'ACGA_G
Cuts at: 667 2224 2582 667
Size: 1557 358 2038
Fragments arranged by size:
2038 1557 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstEII G'GTnAC_C
Cuts at: 468 468
Size: 3953
BstYI r'GATC_y
Cuts at: 1 978 1445 1468 1803 2049 3061 3147 3159
Size: 977 467 23 335 246 1012 86 12
Cuts at: 3159 3505 1
Size: 346 449
Fragments arranged by size:
1012 977 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 872 937 872
Size: 65 3888
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 893 1196 893
Size: 303 3650
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 528 536 786 1538 1712 2103 2130 3475 528
Size: 8 250 752 174 391 27 1345 1006
Fragments arranged by size:
1345 1006 752 391 250 174 27 8
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 240 1976 2186 240
Size: 1736 210 2007
Fragments arranged by size:
2007 1736 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 355 934 957 2161 2471 2617 3763 355
Size: 579 23 1204 310 146 1146 545
Fragments arranged by size:
1204 1146 579 545 310 146 23
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 352 432 755 1778 2210 2957 3225 322
Size: 30 80 323 1023 432 747 268 1050
Fragments arranged by size:
1050 1023 747 432 323 268 80 30
EcoO109I rG'GnC_Cy
Cuts at: 622 815 877 1092 622
Size: 193 62 215 3483
Fragments arranged by size:
3483 215 193 62
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 514 1734 514
Size: 1220 2733
FspAI rTGC'GCAy
Cuts at: 514 514
Size: 3953
HaeII r_GCGC'y
Cuts at: 604 694 710 1387 1635 2657 3636 604
Size: 90 16 677 248 1022 979 921
Fragments arranged by size:
1022 979 921 677 248 90 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1795 1825 1827 1857 1795
Size: 30 2 30 3891
Fragments arranged by size:
3891 30 30 2
HincII GTy'rAC
Cuts at: 171 992 171
Size: 821 3132
KasI G'GCGC_C
Cuts at: 600 690 706 1383 1631 600
Size: 90 16 677 248 2922
Fragments arranged by size:
2922 677 248 90 16
KpnI G_GTAC'C
Cuts at: 775 973 775
Size: 198 3755
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 538 788 1714 538
Size: 250 926 2777
Fragments arranged by size:
2777 926 250
MslI CAynn'nnrTG
Cuts at: 871 1942 2069 871
Size: 1071 127 2755
Fragments arranged by size:
2755 1071 127
MspA1I CmG'CkG
Cuts at: 115 276 384 830 863 902 1064 1378 1738
Size: 161 108 446 33 39 162 314 360
Cuts at: 1738 2751 2996 115
Size: 1013 245 1072
Fragments arranged by size:
1072 1013 446 360 314 245 162 161
108 39 33
NaeI GCC'GGC
Cuts at: 444 2134 444
Size: 1690 2263
NarI GG'CG_CC
Cuts at: 601 691 707 1384 1632 601
Size: 90 16 677 248 2922
Fragments arranged by size:
2922 677 248 90 16
NgoMIV G'CCGG_C
Cuts at: 442 2132 442
Size: 1690 2263
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PmlI CAC'GTG
Cuts at: 488 488
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 622 622
Size: 3953
PspOMI G'GGCC_C
Cuts at: 876 876
Size: 3953
PstI C_TGCA'G
Cuts at: 383 1000 1685 383
Size: 617 685 2651
Fragments arranged by size:
2651 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 115 276 1378 1738 115
Size: 161 1102 360 2330
Fragments arranged by size:
2330 1102 360 161
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SanDI GG'GwC_CC
Cuts at: 622 622
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 240 1976 2186 240
Size: 1736 210 2007
Fragments arranged by size:
2007 1736 210
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 379 996 1681 2674 2865 33
Size: 346 617 685 993 191 1121
Fragments arranged by size:
1121 993 685 617 346 191
SfoI GGC'GCC
Cuts at: 602 692 708 1385 1633 602
Size: 90 16 677 248 2922
Fragments arranged by size:
2922 677 248 90 16
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 576 681 975 576
Size: 105 294 3554
Fragments arranged by size:
3554 294 105
SmlI C'TyrA_G
Cuts at: 1108 2023 2515 2777 1108
Size: 915 492 262 2284
Fragments arranged by size:
2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 1087 1441 1087
Size: 354 3599
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1536 1669 2128 2337 1536
Size: 133 459 209 3152
Fragments arranged by size:
3152 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 913 945 3470 3502 913
Size: 32 2525 32 1364
Fragments arranged by size:
2525 1364 32 32
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XmaI C'CCGG_G
Cuts at: 574 679 973 574
Size: 105 294 3554
Fragments arranged by size:
3554 294 105
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI