Restriction map for
pF1KB7968
(Circular) (Six-base) MAPSORT of: pF1KB7968.seq Check: 254 from: 1 to: 4334
pF1KB7968 4334 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 237 1150 1234 2159 2591 3338 3606 167
Size: 70 913 84 925 432 747 268 895
Fragments arranged by size:
925 913 895 747 432 268 84 70
AflIII A'CryG_T
Cuts at: 1169 1686 2790 3767 1169
Size: 517 1104 977 1736
Fragments arranged by size:
1736 1104 977 517
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1054 1054
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 232 390 579 1639 3206 232
Size: 158 189 1060 1567 1360
Fragments arranged by size:
1567 1360 1060 189 158
ApaI G_GGCC'C
Cuts at: 205 205
Size: 4334
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 1119 1338 3687 3943 1119
Size: 219 2349 256 1510
Fragments arranged by size:
2349 1510 256 219
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 337 419 1354 337
Size: 82 935 3317
Fragments arranged by size:
3317 935 82
AvrII C'CTAG_G
Cuts at: 604 604
Size: 4334
BamHI G'GATC_C
Cuts at: 1229 1359 1229
Size: 130 4204
BanI G'GyrC_C
Cuts at: 1350 1764 2012 2047 1350
Size: 414 248 35 3637
Fragments arranged by size:
3637 414 248 35
BanII G_rGCy'C
Cuts at: 205 239 628 673 1291 1348 2378 205
Size: 34 389 45 618 57 1030 2161
Fragments arranged by size:
2161 1030 618 389 57 45 34
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 299 333 299
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglII A'GATC_T
Cuts at: 612 1849 3886 612
Size: 1237 2037 1060
Fragments arranged by size:
2037 1237 1060
BlpI GC'TnA_GC
Cuts at: 667 1068 1446 667
Size: 401 378 3555
Fragments arranged by size:
3555 401 378
Bme1580I G_kGCm'C
Cuts at: 205 1959 2052 3108 205
Size: 1754 93 1056 1431
Fragments arranged by size:
1754 1431 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1627 1962 4106 1546
Size: 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 893 2701 315
Size: 578 1808 1948
Fragments arranged by size:
1948 1808 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 1259 614
Size: 645 3689
Bpu10I CC'TnA_GC
Cuts at: 233 233
Size: 4334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1155 4038 1155
Size: 2883 1451
BsaAI yAC'GTr
Cuts at: 1115 1172 1687 1115
Size: 57 515 3762
Fragments arranged by size:
3762 515 57
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 1765 2013 2715 4211 1765
Size: 248 702 1496 1888
Fragments arranged by size:
1888 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 197 688 197
Size: 491 3843
BseYI C'CCAG_C
Cuts at: 302 724 1759 2517 3094 3590 302
Size: 422 1035 758 577 496 1046
Fragments arranged by size:
1046 1035 758 577 496 422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 824 1204 328
Size: 496 380 3458
Fragments arranged by size:
3458 496 380
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 239 542 628 1291 1348 2126 2316 3108 239
Size: 303 86 663 57 778 190 792 1465
Fragments arranged by size:
1465 792 778 663 303 190 86 57
Bsp1286I G_dGCh'C
Cuts at: 205 239 542 628 673 1291 1348 1959 2052
Size: 34 303 86 45 618 57 611 93
Cuts at: 2052 2126 2316 2378 3108 205
Size: 74 190 62 730 1431
Fragments arranged by size:
1431 730 618 611 303 190 93 86
74 62 57 45 34
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 885 1384 1598 1900 2281 885
Size: 499 214 302 381 2938
Fragments arranged by size:
2938 499 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1067 2626 2680 4164 4329 50
Size: 1017 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 1017 165 55 54
BsrDI GCAATG_nn'
Cuts at: 942 2246 942
Size: 1304 3030
BsrFI r'CCGG_y
Cuts at: 662 2332 2513 2722 662
Size: 1670 181 209 2274
Fragments arranged by size:
2274 1670 209 181
BssSI C'ACGA_G
Cuts at: 466 2605 2963 466
Size: 2139 358 1837
Fragments arranged by size:
2139 1837 358
BstAPI GCAn_nnn'nTGC
Cuts at: 537 1711 537
Size: 1174 3160
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstEII G'GTnAC_C
Cuts at: 1051 1051
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 506 506
Size: 4334
BstYI r'GATC_y
Cuts at: 1 228 410 556 612 820 1229 1359 1826
Size: 227 182 146 56 208 409 130 467
Cuts at: 1826 1849 2184 2430 3442 3528 3540 3886 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 409 346 335 246 227
208 182 146 130 86 56 23 12
BtgI C'CryG_G
Cuts at: 107 1320 107
Size: 1213 3121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 511 812 1577 511
Size: 301 765 3268
Fragments arranged by size:
3268 765 301
ClaI AT'CG_AT
Cuts at: 432 432
Size: 4334
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 512 1260 1919 2093 2484 2511 3856 512
Size: 748 659 174 391 27 1345 990
Fragments arranged by size:
1345 990 748 659 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 185 1126 2357 2567 185
Size: 941 1231 210 1952
Fragments arranged by size:
1952 1231 941 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 237 626 1289 1346 237
Size: 389 663 57 3225
Fragments arranged by size:
3225 663 389 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 237 614 1150 1234 1259 2159 2591 3338
Size: 70 377 536 84 25 900 432 747
Cuts at: 3338 3606 167
Size: 268 895
Fragments arranged by size:
900 895 747 536 432 377 268 84
70 25
EcoO109I rG'GnC_Cy
Cuts at: 201 202 735 1473 201
Size: 1 533 738 3062
Fragments arranged by size:
3062 738 533 1
EcoRI G'AATT_C
Cuts at: 1119 1338 1119
Size: 219 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 558 2353 558
Size: 1795 2539
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 298 1768 2016 3038 4017 298
Size: 1470 248 1022 979 615
Fragments arranged by size:
1470 1022 979 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 402 434 660 692 2176 2206 2208 2238 402
Size: 32 226 32 1484 30 2 30 2498
Fragments arranged by size:
2498 1484 226 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1208 1373 1208
Size: 165 4169
HindIII A'AGCT_T
Cuts at: 149 1107 1275 149
Size: 958 168 3208
Fragments arranged by size:
3208 958 168
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 333 3005 3189 164
Size: 169 2672 184 1309
Fragments arranged by size:
2672 1309 184 169
MscI TGG'CCA
Cuts at: 1262 2095 1262
Size: 833 3501
MslI CAynn'nnrTG
Cuts at: 1254 2323 2450 1254
Size: 1069 127 3138
Fragments arranged by size:
3138 1069 127
MspA1I CmG'CkG
Cuts at: 814 1445 1759 2119 3132 3377 814
Size: 631 314 360 1013 245 1771
Fragments arranged by size:
1771 1013 631 360 314 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 1320 107
Size: 1213 3121
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 1387 1887 2794 1387
Size: 500 907 2927
Fragments arranged by size:
2927 907 500
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PmlI CAC'GTG
Cuts at: 1115 1172 1115
Size: 57 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PspOMI G'GGCC_C
Cuts at: 201 201
Size: 4334
PstI C_TGCA'G
Cuts at: 842 1381 2066 842
Size: 539 685 3110
Fragments arranged by size:
3110 685 539
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 814 1759 2119 814
Size: 945 360 3029
Fragments arranged by size:
3029 945 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 239 628 1291 1348 239
Size: 389 663 57 3225
Fragments arranged by size:
3225 663 389 57
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 838 1377 2062 3055 3246 33
Size: 805 539 685 993 191 1121
Fragments arranged by size:
1121 993 805 685 539 191
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StyI C'CwwG_G
Cuts at: 107 350 604 1320 1468 1822 107
Size: 243 254 716 148 354 2619
Fragments arranged by size:
2619 716 354 254 243 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 498 998 1917 2050 2509 2718 498
Size: 500 919 133 459 209 2114
Fragments arranged by size:
2114 919 500 459 209 133
TatI w'GTAC_w
Cuts at: 3849 3849
Size: 4334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 3851 3883 472
Size: 32 3347 32 923
Fragments arranged by size:
3347 923 32 32
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
XmnI GAAnn'nnTTC
Cuts at: 476 1108 476
Size: 632 3702
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI