Restriction map for
pF1KB7969
(Circular) (Six-base) MAPSORT of: pF1KB7969.seq Check: 9470 from: 1 to: 3968
pF1KB7969 3968 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 314 1006 314
Size: 692 3276
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1793 2225 2972 3240 653
Size: 1140 432 747 268 1381
Fragments arranged by size:
1381 1140 747 432 268
AflIII A'CryG_T
Cuts at: 425 1320 2424 3401 425
Size: 895 1104 977 992
Fragments arranged by size:
1104 992 977 895
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 793 1273 2840 793
Size: 480 1567 1921
Fragments arranged by size:
1921 1567 480
ApaLI G'TGCA_C
Cuts at: 2738 2738
Size: 3968
ApoI r'AATT_y
Cuts at: 140 185 265 406 972 3321 3577 140
Size: 45 80 141 566 2349 256 531
Fragments arranged by size:
2349 566 531 256 141 80 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 988 988
Size: 3968
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 984 1398 1646 1681 984
Size: 414 248 35 3271
Fragments arranged by size:
3271 414 248 35
BanII G_rGCy'C
Cuts at: 982 2012 982
Size: 1030 2938
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3934
BciVI GTATCCnnnnn_n'
Cuts at: 478 1866 2627 3968 478
Size: 1388 761 1341 478
Fragments arranged by size:
1388 1341 761 478
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BfrBI ATG'CAT
Cuts at: 934 934
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 1593 1686 2742 1593
Size: 93 1056 2819
Fragments arranged by size:
2819 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 501 1180 1261 1596 3740 501
Size: 679 81 335 2144 729
Fragments arranged by size:
2144 729 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 2335 562
Size: 1773 2195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 557 359
Size: 198 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1157 1678 2356 2630 2777 3188 1157
Size: 521 678 274 147 411 1937
Fragments arranged by size:
1937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 442 412
Size: 30 3938
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 502
Size: 3968
BseYI C'CCAG_C
Cuts at: 1393 2151 2728 3224 1393
Size: 758 577 496 2137
Fragments arranged by size:
2137 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 982 1760 1950 2742 982
Size: 778 190 792 2208
Fragments arranged by size:
2208 792 778 190
BsmI GAATG_Cn'
Cuts at: 936 936
Size: 3968
Bsp1286I G_dGCh'C
Cuts at: 982 1593 1686 1760 1950 2012 2742 982
Size: 611 93 74 190 62 730 2208
Fragments arranged by size:
2208 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3188 3188
Size: 3968
BspHI T'CATG_A
Cuts at: 701 3144 701
Size: 2443 1525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1018 1232 1534 1915 1018
Size: 214 302 381 3071
Fragments arranged by size:
3071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2260 2314 3798 3963 50
Size: 2210 54 1484 165 55
Fragments arranged by size:
2210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 282 799 1880 282
Size: 517 1081 2370
Fragments arranged by size:
2370 1081 517
BsrFI r'CCGG_y
Cuts at: 1966 2147 2356 1966
Size: 181 209 3578
Fragments arranged by size:
3578 209 181
BsrGI T'GTAC_A
Cuts at: 912 912
Size: 3968
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstYI r'GATC_y
Cuts at: 1 656 790 993 1460 1483 1818 2064 3076
Size: 655 134 203 467 23 335 246 1012
Cuts at: 3076 3162 3174 3520 1
Size: 86 12 346 449
Fragments arranged by size:
1012 655 467 449 346 335 246 203
134 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1211
Size: 3968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 855 890 855
Size: 35 3933
DraI TTT'AAA
Cuts at: 405 477 967 405
Size: 72 490 3406
Fragments arranged by size:
3406 490 72
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 1553 1727 2118 2145 3490 1553
Size: 174 391 27 1345 2031
Fragments arranged by size:
2031 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 167 700 935 1991 2201 167
Size: 533 235 1056 210 1934
Fragments arranged by size:
1934 1056 533 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 557 653 1793 2225 2972 3240 359
Size: 198 96 1140 432 747 268 1087
Fragments arranged by size:
1140 1087 747 432 268 198 96
EcoO109I rG'GnC_Cy
Cuts at: 1107 1107
Size: 3968
EcoRI G'AATT_C
Cuts at: 265 972 265
Size: 707 3261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 658 1987 658
Size: 1329 2639
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 1402 1650 2672 3651 1402
Size: 248 1022 979 1719
Fragments arranged by size:
1719 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1810 1840 1842 1872 1810
Size: 30 2 30 3906
Fragments arranged by size:
3906 30 30 2
HincII GTy'rAC
Cuts at: 1007 1007
Size: 3968
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2639 2823 2639
Size: 184 3784
MscI TGG'CCA
Cuts at: 1729 1729
Size: 3968
MslI CAynn'nnrTG
Cuts at: 811 857 884 1957 2084 811
Size: 46 27 1073 127 2695
Fragments arranged by size:
2695 1073 127 46 27
MspA1I CmG'CkG
Cuts at: 115 1079 1393 1753 2766 3011 115
Size: 964 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 964 360 314 245
NaeI GCC'GGC
Cuts at: 2149 2149
Size: 3968
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NdeI CA'TA_TG
Cuts at: 936 936
Size: 3968
NgoMIV G'CCGG_C
Cuts at: 2147 2147
Size: 3968
NsiI A_TGCA'T
Cuts at: 936 936
Size: 3968
NspI r_CATG'y
Cuts at: 429 1021 1521 2428 429
Size: 592 500 907 1969
Fragments arranged by size:
1969 907 592 500
PciI A'CATG_T
Cuts at: 425 2424 425
Size: 1999 1969
PfoI T'CCnGG_A
Cuts at: 375 375
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PstI C_TGCA'G
Cuts at: 1015 1700 1015
Size: 685 3283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 1393 1753 1393
Size: 360 3608
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 700 1991 2201 700
Size: 1291 210 2467
Fragments arranged by size:
2467 1291 210
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 511 3485 511
Size: 2974 994
SfcI C'TryA_G
Cuts at: 33 122 443 1011 1696 2689 2880 33
Size: 89 321 568 685 993 191 1121
Fragments arranged by size:
1121 993 685 568 321 191 89
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
SspI AAT'ATT
Cuts at: 871 871
Size: 3968
StyI C'CwwG_G
Cuts at: 891 1102 1456 891
Size: 211 354 3403
Fragments arranged by size:
3403 354 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1551 1684 2143 2352 1551
Size: 133 459 209 3167
Fragments arranged by size:
3167 459 209 133
TatI w'GTAC_w
Cuts at: 509 912 3483 509
Size: 403 2571 994
Fragments arranged by size:
2571 994 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 3485 3517 219
Size: 32 3234 32 670
Fragments arranged by size:
3234 670 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1463 1463
Size: 3968
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI