Restriction map for
pF1KB7970
(Circular) (Six-base) MAPSORT of: pF1KB7970.seq Check: 3543 from: 1 to: 4343
pF1KB7970 4343 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1381 1381
Size: 4343
Acc65I G'GTAC_C
Cuts at: 546 1359 546
Size: 813 3530
AclI AA'CG_TT
Cuts at: 921 4183 921
Size: 3262 1081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1015 2168 2600 3347 3615 845
Size: 170 1153 432 747 268 1573
Fragments arranged by size:
1573 1153 747 432 268 170
AflIII A'CryG_T
Cuts at: 534 1695 2799 3776 534
Size: 1161 1104 977 1101
Fragments arranged by size:
1161 1104 1101 977
AgeI A'CCGG_T
Cuts at: 2731 2731
Size: 4343
AlwNI CAG_nnn'CTG
Cuts at: 946 1648 3215 946
Size: 702 1567 2074
Fragments arranged by size:
2074 1567 702
ApaI G_GGCC'C
Cuts at: 1287 1287
Size: 4343
ApaLI G'TGCA_C
Cuts at: 243 3113 243
Size: 2870 1473
ApoI r'AATT_y
Cuts at: 469 986 1347 3696 3952 469
Size: 517 361 2349 256 860
Fragments arranged by size:
2349 860 517 361 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 1363 1363
Size: 4343
BamHI G'GATC_C
Cuts at: 708 1368 708
Size: 660 3683
BanI G'GyrC_C
Cuts at: 546 1359 1773 2021 2056 546
Size: 813 414 248 35 2833
Fragments arranged by size:
2833 813 414 248 35
BanII G_rGCy'C
Cuts at: 701 719 1287 1357 2387 701
Size: 18 568 70 1030 2657
Fragments arranged by size:
2657 1030 568 70 18
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BbsI GAAGACnn'nnnn_
Cuts at: 876 876
Size: 4343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4309
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3002 4343 2241
Size: 761 1341 2241
Fragments arranged by size:
2241 1341 761
BclI T'GATC_A
Cuts at: 1863 1863
Size: 4343
BfrBI ATG'CAT
Cuts at: 1151 1151
Size: 4343
BglII A'GATC_T
Cuts at: 1858 3895 1858
Size: 2037 2306
BlpI GC'TnA_GC
Cuts at: 1455 1455
Size: 4343
Bme1580I G_kGCm'C
Cuts at: 247 1287 1968 2061 3117 247
Size: 1040 681 93 1056 1473
Fragments arranged by size:
1473 1056 1040 681 93
BmrI ACTGGGnnnn_n'
Cuts at: 598 1555 1636 1971 4115 598
Size: 957 81 335 2144 826
Fragments arranged by size:
2144 957 826 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4343
Bpu10I CC'TnA_GC
Cuts at: 713 713
Size: 4343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1519 2398 2890 3188 536
Size: 983 879 492 298 1691
Fragments arranged by size:
1691 983 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4047 4047
Size: 4343
BsaAI yAC'GTr
Cuts at: 1696 1696
Size: 4343
BsaBI GATnn'nnATC
Cuts at: 6 1857 3894 6
Size: 1851 2037 455
Fragments arranged by size:
2037 1851 455
BsaHI Gr'CG_yC
Cuts at: 1774 2022 2724 4220 1774
Size: 248 702 1496 1897
Fragments arranged by size:
1897 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1532 2053 2731 3005 3152 3563 1532
Size: 521 678 274 147 411 2312
Fragments arranged by size:
2312 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 798 798
Size: 4343
BseYI C'CCAG_C
Cuts at: 1768 2526 3103 3599 1768
Size: 758 577 496 2512
Fragments arranged by size:
2512 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1885 1931 3139 104
Size: 1781 46 1208 1308
Fragments arranged by size:
1781 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 247 719 1357 2135 2325 3117 247
Size: 472 638 778 190 792 1473
Fragments arranged by size:
1473 792 778 638 472 190
Bsp1286I G_dGCh'C
Cuts at: 247 701 719 1287 1357 1968 2061 2135 2325
Size: 454 18 568 70 611 93 74 190
Cuts at: 2325 2387 3117 247
Size: 62 730 1473
Fragments arranged by size:
1473 730 611 568 454 190 93 74
70 62 18
BspEI T'CCGG_A
Cuts at: 3563 3563
Size: 4343
BspHI T'CATG_A
Cuts at: 3519 3519
Size: 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1393 1607 1909 2290 1393
Size: 214 302 381 3446
Fragments arranged by size:
3446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2635 2689 4173 4338 50
Size: 2585 54 1484 165 55
Fragments arranged by size:
2585 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 822 2255 262
Size: 560 1433 2350
Fragments arranged by size:
2350 1433 560
BsrFI r'CCGG_y
Cuts at: 2341 2522 2731 2341
Size: 181 209 3953
Fragments arranged by size:
3953 209 181
BssSI C'ACGA_G
Cuts at: 2614 2972 2614
Size: 358 3985
BstAPI GCAn_nnn'nTGC
Cuts at: 1720 1720
Size: 4343
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 1305 1305
Size: 4343
BstYI r'GATC_y
Cuts at: 1 708 1368 1835 1858 2193 2439 3451 3537
Size: 707 660 467 23 335 246 1012 86
Cuts at: 3537 3549 3895 1
Size: 12 346 449
Fragments arranged by size:
1012 707 660 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1123 1123
Size: 4343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2293 2474 2637 2293
Size: 181 163 3999
Fragments arranged by size:
3999 181 163
BtsI GCAGTG_nn'
Cuts at: 276 1041 1135 1586 276
Size: 765 94 451 3033
Fragments arranged by size:
3033 765 451 94
DraI TTT'AAA
Cuts at: 153 1342 153
Size: 1189 3154
DrdI GACnn_nn'nnGTC
Cuts at: 2049 2907 2049
Size: 858 3485
EaeI y'GGCC_r
Cuts at: 1065 1928 2102 2493 2520 3865 1065
Size: 863 174 391 27 1345 1543
Fragments arranged by size:
1543 1345 863 391 174 27
EagI C'GGCC_G
Cuts at: 1928 1928
Size: 4343
EarI CTCTTCn'nnn_
Cuts at: 504 2366 2576 504
Size: 1862 210 2271
Fragments arranged by size:
2271 1862 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2551 2861 3007 4153 2551
Size: 310 146 1146 2741
Fragments arranged by size:
2741 1146 310 146
EcoICRI GAG'CTC
Cuts at: 717 1355 717
Size: 638 3705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1015 2168 2600 3347 3615 845
Size: 170 1153 432 747 268 1573
Fragments arranged by size:
1573 1153 747 432 268 170
EcoNI CCTnn'n_nnAGG
Cuts at: 153 153
Size: 4343
EcoO109I rG'GnC_Cy
Cuts at: 1284 1482 1284
Size: 198 4145
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 530 2362 530
Size: 1832 2511
FspI TGC'GCA
Cuts at: 268 2124 268
Size: 1856 2487
HaeII r_GCGC'y
Cuts at: 1777 2025 3047 4026 1777
Size: 248 1022 979 2094
Fragments arranged by size:
2094 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 793 825 2185 2215 2217 2247 793
Size: 32 1360 30 2 30 2889
Fragments arranged by size:
2889 1360 32 30 30 2
HincII GTy'rAC
Cuts at: 355 1382 355
Size: 1027 3316
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 550 1363 550
Size: 813 3530
MluI A'CGCG_T
Cuts at: 3776 3776
Size: 4343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1034 3014 3198 1034
Size: 1980 184 2179
Fragments arranged by size:
2179 1980 184
MscI TGG'CCA
Cuts at: 1067 2104 1067
Size: 1037 3306
MslI CAynn'nnrTG
Cuts at: 1128 2332 2459 1128
Size: 1204 127 3012
Fragments arranged by size:
3012 1204 127
MspA1I CmG'CkG
Cuts at: 1281 1454 1768 2128 3141 3386 1281
Size: 173 314 360 1013 245 2238
Fragments arranged by size:
2238 1013 360 314 245 173
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 4343
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 1123 1123
Size: 4343
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 4343
NsiI A_TGCA'T
Cuts at: 1153 1153
Size: 4343
NspI r_CATG'y
Cuts at: 319 538 1151 1396 1896 2803 319
Size: 219 613 245 500 907 1859
Fragments arranged by size:
1859 907 613 500 245 219
PciI A'CATG_T
Cuts at: 534 2799 534
Size: 2265 2078
PfoI T'CCnGG_A
Cuts at: 629 629
Size: 4343
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 4311
PshAI GACnn'nnGTC
Cuts at: 1274 1274
Size: 4343
PsiI TTA'TAA
Cuts at: 374 641 374
Size: 267 4076
PspOMI G'GGCC_C
Cuts at: 1283 1283
Size: 4343
PstI C_TGCA'G
Cuts at: 1390 2075 1390
Size: 685 3658
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 1768 2128 1768
Size: 360 3983
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 719 1357 719
Size: 638 3705
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 504 2366 2576 504
Size: 1862 210 2271
Fragments arranged by size:
2271 1862 210
SbfI CC_TGCA'GG
Cuts at: 1390 1390
Size: 4343
ScaI AGT'ACT
Cuts at: 3860 3860
Size: 4343
SfcI C'TryA_G
Cuts at: 33 1386 2071 3064 3255 33
Size: 1353 685 993 191 1121
Fragments arranged by size:
1353 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 1365 1365
Size: 4343
SmlI C'TyrA_G
Cuts at: 551 1498 2413 2905 3167 551
Size: 947 915 492 262 1727
Fragments arranged by size:
1727 947 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 1396 1896 1396
Size: 500 3843
SspI AAT'ATT
Cuts at: 133 637 133
Size: 504 3839
StyI C'CwwG_G
Cuts at: 1123 1236 1477 1831 1123
Size: 113 241 354 3635
Fragments arranged by size:
3635 354 241 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 1311 1926 2059 2518 2727 127
Size: 1184 615 133 459 209 1743
Fragments arranged by size:
1743 1184 615 459 209 133
TatI w'GTAC_w
Cuts at: 560 927 3858 560
Size: 367 2931 1045
Fragments arranged by size:
2931 1045 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 3860 3892 524
Size: 32 3304 32 975
Fragments arranged by size:
3304 975 32 32
Tth111I GACn'n_nGTC
Cuts at: 2140 2140
Size: 4343
XbaI T'CTAG_A
Cuts at: 726 1374 726
Size: 648 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1838 1256
Size: 582 3761
XmaI C'CCGG_G
Cuts at: 1363 1363
Size: 4343
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI