Restriction map for pF1KB7971
 (Circular) (Six-base) MAPSORT of: pF1KB7971.seq  Check: 5605  from: 1  to: 3983



pF1KB7971  3983 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:    252    1021     252 
   Size:        769    3214
 
Acc65I G'GTAC_C

Cuts at:    999     999 
   Size:       3983
 
AclI AA'CG_TT

Cuts at:    977    3823     977 
   Size:       2846    1137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1808    2240    2987    3255    1808 
   Size:        432     747     268    2536

  Fragments arranged by size: 

                2536     747     432     268
 
AflIII A'CryG_T

Cuts at:   1335    2439    3416    1335 
   Size:       1104     977    1902

  Fragments arranged by size: 

                1902    1104     977
 
AgeI A'CCGG_T

Cuts at:   2371    2371 
   Size:       3983
 
AleI CACnn'nnGTG

Cuts at:    760     760 
   Size:       3983
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    842     874     842 
   Size:         32    3951
 
AlwNI CAG_nnn'CTG

Cuts at:    507     556    1288    2855     507 
   Size:         49     732    1567    1635

  Fragments arranged by size: 

                1635    1567     732      49
 
ApaI G_GGCC'C

Cuts at:    722     722 
   Size:       3983
 
ApaLI G'TGCA_C

Cuts at:   2753    2753 
   Size:       3983
 
ApoI r'AATT_y

Cuts at:    238     987    3336    3592     238 
   Size:        749    2349     256     629

  Fragments arranged by size: 

                2349     749     629     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3983
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3983
 
AvaI C'yCGr_G

Cuts at:    199    1003     199 
   Size:        804    3179
 
BamHI G'GATC_C

Cuts at:    216     801    1008     216 
   Size:        585     207    3191

  Fragments arranged by size: 

                3191     585     207
 
BanI G'GyrC_C

Cuts at:    999    1413    1661    1696     999 
   Size:        414     248      35    3286

  Fragments arranged by size: 

                3286     414     248      35
 
BanII G_rGCy'C

Cuts at:    475     722     997    2027     475 
   Size:        247     275    1030    2431

  Fragments arranged by size: 

                2431    1030     275     247
 
BbeI G_GCGC'C

Cuts at:   1417    1665    1417 
   Size:        248    3735
 
BbsI GAAGACnn'nnnn_

Cuts at:    341     341 
   Size:       3983
 
BciVI GTATCCnnnnn_n'

Cuts at:    309    1881    2642    3983     309 
   Size:       1572     761    1341     309

  Fragments arranged by size: 

                1572    1341     761     309
 
BclI T'GATC_A

Cuts at:    270    1503     270 
   Size:       1233    2750
 
BglII A'GATC_T

Cuts at:   1498    3535    1498 
   Size:       2037    1946
 
BlpI GC'TnA_GC

Cuts at:    142    1095     142 
   Size:        953    3030
 
Bme1580I G_kGCm'C

Cuts at:    722    1608    1701    2757     722 
   Size:        886      93    1056    1948

  Fragments arranged by size: 

                1948    1056     886      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    527    1195    1276    1611    3755     527 
   Size:        668      81     335    2144     755

  Fragments arranged by size: 

                2144     755     668     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2350    2350 
   Size:       3983
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     722     587 
   Size:        135    3848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    887    1159    2038    2530    2828     887 
   Size:        272     879     492     298    2042

  Fragments arranged by size: 

                2042     879     492     298     272
 
BsaI GGTCTCn'nnnn_

Cuts at:   3687    3687 
   Size:       3983
 
BsaAI yAC'GTr

Cuts at:   1336    1336 
   Size:       3983
 
BsaBI GATnn'nnATC

Cuts at:      6    1497    3534       6 
   Size:       1491    2037     455

  Fragments arranged by size: 

                2037    1491     455
 
BsaHI Gr'CG_yC

Cuts at:   1414    1662    2364    3860    1414 
   Size:        248     702    1496    1537

  Fragments arranged by size: 

                1537    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    179    1172    1693    2371    2645    2792    3203     179 
   Size:        993     521     678     274     147     411     959

  Fragments arranged by size: 

                 993     959     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    161     191     481     511     161 
   Size:         30     290      30    3633

  Fragments arranged by size: 

                3633     290      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    445     483     445 
   Size:         38    3945
 
BseYI C'CCAG_C

Cuts at:    832    1408    2166    2743    3239     832 
   Size:        576     758     577     496    1576

  Fragments arranged by size: 

                1576     758     577     576     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    391     391 
   Size:       3983
 
BsiEI CG_ry'CG

Cuts at:    104    1525    1571    2779     104 
   Size:       1421      46    1208    1308

  Fragments arranged by size: 

                1421    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    143     475     997    1775    1965    2757     143 
   Size:        332     522     778     190     792    1369

  Fragments arranged by size: 

                1369     792     778     522     332     190
 
BsmI GAATG_Cn'

Cuts at:    401     401 
   Size:       3983
 
Bsp1286I G_dGCh'C

Cuts at:    143     475     722     997    1608    1701    1775    1965    2027 
   Size:        332     247     275     611      93      74     190      62

Cuts at:   2027    2757     143 
   Size:        730    1369

  Fragments arranged by size: 

                1369     730     611     332     275     247     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:    179    3203     179 
   Size:       3024     959
 
BspHI T'CATG_A

Cuts at:   3159    3159 
   Size:       3983
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1033    1247    1549    1930    1033 
   Size:        214     302     381    3086

  Fragments arranged by size: 

                3086     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2275    2329    3813    3978      50 
   Size:       2225      54    1484     165      55

  Fragments arranged by size: 

                2225    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1895    1895 
   Size:       3983
 
BsrFI r'CCGG_y

Cuts at:   1981    2162    2371    1981 
   Size:        181     209    3593

  Fragments arranged by size: 

                3593     209     181
 
BssSI C'ACGA_G

Cuts at:   2254    2612    2254 
   Size:        358    3625
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1360    1360 
   Size:       3983
 
BstBI TT'CG_AA

Cuts at:   2344    2344 
   Size:       3983
 
BstYI r'GATC_y

Cuts at:      1     216     801    1008    1475    1498    1833    2079    3091 
   Size:        215     585     207     467      23     335     246    1012

Cuts at:   3091    3177    3189    3535       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     585     467     449     346     335     246     215
                 207      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     441     107 
   Size:        334    3649
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1933    2114    2277    1933 
   Size:        181     163    3639

  Fragments arranged by size: 

                3639     181     163
 
BtsI GCAGTG_nn'

Cuts at:    385     767    1226     385 
   Size:        382     459    3142

  Fragments arranged by size: 

                3142     459     382
 
DraI TTT'AAA

Cuts at:    243     982     243 
   Size:        739    3244
 
DrdI GACnn_nn'nnGTC

Cuts at:   1689    2547    1689 
   Size:        858    3125
 
EaeI y'GGCC_r

Cuts at:   1568    1742    2133    2160    3505    1568 
   Size:        174     391      27    1345    2046

  Fragments arranged by size: 

                2046    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1568    1568 
   Size:       3983
 
EarI CTCTTCn'nnn_

Cuts at:    887    2006    2216     887 
   Size:       1119     210    2654

  Fragments arranged by size: 

                2654    1119     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2191    2501    2647    3793    2191 
   Size:        310     146    1146    2381

  Fragments arranged by size: 

                2381    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    473     995     473 
   Size:        522    3461
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     722    1808    2240    2987    3255     587 
   Size:        135    1086     432     747     268    1315

  Fragments arranged by size: 

                1315    1086     747     432     268     135
 
EcoO109I rG'GnC_Cy

Cuts at:    113    1122     113 
   Size:       1009    2974
 
EcoRI G'AATT_C

Cuts at:    987     987 
   Size:       3983
 
EcoRV GAT'ATC

Cuts at:    698     698 
   Size:       3983
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    928    2002     928 
   Size:       1074    2909
 
FspI TGC'GCA

Cuts at:   1764    1764 
   Size:       3983
 
HaeII r_GCGC'y

Cuts at:   1417    1665    2687    3666    1417 
   Size:        248    1022     979    1734

  Fragments arranged by size: 

                1734    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    161     193    1825    1855    1857    1887     161 
   Size:         32    1632      30       2      30    2257

  Fragments arranged by size: 

                2257    1632      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1022    1022 
   Size:       3983
 
HindIII A'AGCT_T

Cuts at:    681     681 
   Size:       3983
 
KasI G'GCGC_C

Cuts at:   1413    1661    1413 
   Size:        248    3735
 
KpnI G_GTAC'C

Cuts at:   1003    1003 
   Size:       3983
 
MluI A'CGCG_T

Cuts at:   3416    3416 
   Size:       3983
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    137     147     885    2654    2838     137 
   Size:         10     738    1769     184    1282

  Fragments arranged by size: 

                1769    1282     738     184      10
 
MscI TGG'CCA

Cuts at:   1744    1744 
   Size:       3983
 
MslI CAynn'nnrTG

Cuts at:    760    1972    2099     760 
   Size:       1212     127    2644

  Fragments arranged by size: 

                2644    1212     127
 
MspA1I CmG'CkG

Cuts at:   1094    1408    1768    2781    3026    1094 
   Size:        314     360    1013     245    2051

  Fragments arranged by size: 

                2051    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2164    2164 
   Size:       3983
 
NarI GG'CG_CC

Cuts at:   1414    1662    1414 
   Size:        248    3735
 
NcoI C'CATG_G

Cuts at:    107     441     107 
   Size:        334    3649
 
NgoMIV G'CCGG_C

Cuts at:   2162    2162 
   Size:       3983
 
NspI r_CATG'y

Cuts at:   1036    1536    2443    1036 
   Size:        500     907    2576

  Fragments arranged by size: 

                2576     907     500
 
PasI CC'CwG_GG

Cuts at:    576     576 
   Size:       3983
 
PciI A'CATG_T

Cuts at:   2439    2439 
   Size:       3983
 
PflMI CCAn_nnn'nTGG

Cuts at:    442     956     442 
   Size:        514    3469
 
PfoI T'CCnGG_A

Cuts at:    308     308 
   Size:       3983
 
PmeI GTTT'AAAC

Cuts at:    982     982 
   Size:       3983
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3122    3154    3122 
   Size:         32    3951
 
PpuMI rG'GwC_Cy

Cuts at:    113     113 
   Size:       3983
 
PspOMI G'GGCC_C

Cuts at:    718     718 
   Size:       3983
 
PstI C_TGCA'G

Cuts at:   1030    1715    1030 
   Size:        685    3298
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3983
 
PvuII CAG'CTG

Cuts at:   1408    1768    1408 
   Size:        360    3623
 
RsrII CG'GwC_CG

Cuts at:   2178    2178 
   Size:       3983
 
SacI G_AGCT'C

Cuts at:    475     997     475 
   Size:        522    3461
 
SalI G'TCGA_C

Cuts at:   1020    1020 
   Size:       3983
 
SapI GCTCTTCn'nnn_

Cuts at:   2006    2216    2006 
   Size:        210    3773
 
SbfI CC_TGCA'GG

Cuts at:   1030    1030 
   Size:       3983
 
ScaI AGT'ACT

Cuts at:   3500    3500 
   Size:       3983
 
SfcI C'TryA_G

Cuts at:     33    1026    1711    2704    2895      33 
   Size:        993     685     993     191    1121

  Fragments arranged by size: 

                1121     993     993     685     191
 
SfoI GGC'GCC

Cuts at:   1415    1663    1415 
   Size:        248    3735
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3983
 
SmaI CCC'GGG

Cuts at:   1005    1005 
   Size:       3983
 
SmlI C'TyrA_G

Cuts at:    902    1138    2053    2545    2807     902 
   Size:        236     915     492     262    2078

  Fragments arranged by size: 

                2078     915     492     262     236
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3983
 
SphI G_CATG'C

Cuts at:   1036    1536    1036 
   Size:        500    3483
 
StyI C'CwwG_G

Cuts at:    107     441     590    1117    1471     107 
   Size:        334     149     527     354    2619

  Fragments arranged by size: 

                2619     527     354     334     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1566    1699    2158    2367    1566 
   Size:        133     459     209    3182

  Fragments arranged by size: 

                3182     459     209     133
 
TatI w'GTAC_w

Cuts at:   3498    3498 
   Size:       3983
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3500    3532    3500 
   Size:         32    3951
 
Tth111I GACn'n_nGTC

Cuts at:    781    1780     781 
   Size:        999    2984
 
XbaI T'CTAG_A

Cuts at:   1014    1014 
   Size:       3983
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1478    1478 
   Size:       3983
 
XmaI C'CCGG_G

Cuts at:   1003    1003 
   Size:       3983
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI   Bpu10I
    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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