(Circular) (Six-base) MAPSORT of: pF1KB7971.seq Check: 5605 from: 1 to: 3983 pF1KB7971 3983 bp With 182 enzymes: SgfI * October 7, 2009 14:25 .. AccI GT'mk_AC Cuts at: 252 1021 252 Size: 769 3214 Acc65I G'GTAC_C Cuts at: 999 999 Size: 3983 AclI AA'CG_TT Cuts at: 977 3823 977 Size: 2846 1137 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1808 2240 2987 3255 1808 Size: 432 747 268 2536 Fragments arranged by size: 2536 747 432 268 AflIII A'CryG_T Cuts at: 1335 2439 3416 1335 Size: 1104 977 1902 Fragments arranged by size: 1902 1104 977 AgeI A'CCGG_T Cuts at: 2371 2371 Size: 3983 AleI CACnn'nnGTG Cuts at: 760 760 Size: 3983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 842 874 842 Size: 32 3951 AlwNI CAG_nnn'CTG Cuts at: 507 556 1288 2855 507 Size: 49 732 1567 1635 Fragments arranged by size: 1635 1567 732 49 ApaI G_GGCC'C Cuts at: 722 722 Size: 3983 ApaLI G'TGCA_C Cuts at: 2753 2753 Size: 3983 ApoI r'AATT_y Cuts at: 238 987 3336 3592 238 Size: 749 2349 256 629 Fragments arranged by size: 2349 749 629 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3983 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3983 AvaI C'yCGr_G Cuts at: 199 1003 199 Size: 804 3179 BamHI G'GATC_C Cuts at: 216 801 1008 216 Size: 585 207 3191 Fragments arranged by size: 3191 585 207 BanI G'GyrC_C Cuts at: 999 1413 1661 1696 999 Size: 414 248 35 3286 Fragments arranged by size: 3286 414 248 35 BanII G_rGCy'C Cuts at: 475 722 997 2027 475 Size: 247 275 1030 2431 Fragments arranged by size: 2431 1030 275 247 BbeI G_GCGC'C Cuts at: 1417 1665 1417 Size: 248 3735 BbsI GAAGACnn'nnnn_ Cuts at: 341 341 Size: 3983 BciVI GTATCCnnnnn_n' Cuts at: 309 1881 2642 3983 309 Size: 1572 761 1341 309 Fragments arranged by size: 1572 1341 761 309 BclI T'GATC_A Cuts at: 270 1503 270 Size: 1233 2750 BglII A'GATC_T Cuts at: 1498 3535 1498 Size: 2037 1946 BlpI GC'TnA_GC Cuts at: 142 1095 142 Size: 953 3030 Bme1580I G_kGCm'C Cuts at: 722 1608 1701 2757 722 Size: 886 93 1056 1948 Fragments arranged by size: 1948 1056 886 93 BmrI ACTGGGnnnn_n' Cuts at: 527 1195 1276 1611 3755 527 Size: 668 81 335 2144 755 Fragments arranged by size: 2144 755 668 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 3983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 587 722 587 Size: 135 3848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 887 1159 2038 2530 2828 887 Size: 272 879 492 298 2042 Fragments arranged by size: 2042 879 492 298 272 BsaI GGTCTCn'nnnn_ Cuts at: 3687 3687 Size: 3983 BsaAI yAC'GTr Cuts at: 1336 1336 Size: 3983 BsaBI GATnn'nnATC Cuts at: 6 1497 3534 6 Size: 1491 2037 455 Fragments arranged by size: 2037 1491 455 BsaHI Gr'CG_yC Cuts at: 1414 1662 2364 3860 1414 Size: 248 702 1496 1537 Fragments arranged by size: 1537 1496 702 248 BsaWI w'CCGG_w Cuts at: 179 1172 1693 2371 2645 2792 3203 179 Size: 993 521 678 274 147 411 959 Fragments arranged by size: 993 959 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 161 191 481 511 161 Size: 30 290 30 3633 Fragments arranged by size: 3633 290 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 445 483 445 Size: 38 3945 BseYI C'CCAG_C Cuts at: 832 1408 2166 2743 3239 832 Size: 576 758 577 496 1576 Fragments arranged by size: 1576 758 577 576 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 391 391 Size: 3983 BsiEI CG_ry'CG Cuts at: 104 1525 1571 2779 104 Size: 1421 46 1208 1308 Fragments arranged by size: 1421 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 143 475 997 1775 1965 2757 143 Size: 332 522 778 190 792 1369 Fragments arranged by size: 1369 792 778 522 332 190 BsmI GAATG_Cn' Cuts at: 401 401 Size: 3983 Bsp1286I G_dGCh'C Cuts at: 143 475 722 997 1608 1701 1775 1965 2027 Size: 332 247 275 611 93 74 190 62 Cuts at: 2027 2757 143 Size: 730 1369 Fragments arranged by size: 1369 730 611 332 275 247 190 93 74 62 BspEI T'CCGG_A Cuts at: 179 3203 179 Size: 3024 959 BspHI T'CATG_A Cuts at: 3159 3159 Size: 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1033 1247 1549 1930 1033 Size: 214 302 381 3086 Fragments arranged by size: 3086 381 302 214 BsrBI CCG'CTC Cuts at: 50 2275 2329 3813 3978 50 Size: 2225 54 1484 165 55 Fragments arranged by size: 2225 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1895 1895 Size: 3983 BsrFI r'CCGG_y Cuts at: 1981 2162 2371 1981 Size: 181 209 3593 Fragments arranged by size: 3593 209 181 BssSI C'ACGA_G Cuts at: 2254 2612 2254 Size: 358 3625 BstAPI GCAn_nnn'nTGC Cuts at: 1360 1360 Size: 3983 BstBI TT'CG_AA Cuts at: 2344 2344 Size: 3983 BstYI r'GATC_y Cuts at: 1 216 801 1008 1475 1498 1833 2079 3091 Size: 215 585 207 467 23 335 246 1012 Cuts at: 3091 3177 3189 3535 1 Size: 86 12 346 449 Fragments arranged by size: 1012 585 467 449 346 335 246 215 207 86 23 12 BtgI C'CryG_G Cuts at: 107 441 107 Size: 334 3649 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1933 2114 2277 1933 Size: 181 163 3639 Fragments arranged by size: 3639 181 163 BtsI GCAGTG_nn' Cuts at: 385 767 1226 385 Size: 382 459 3142 Fragments arranged by size: 3142 459 382 DraI TTT'AAA Cuts at: 243 982 243 Size: 739 3244 DrdI GACnn_nn'nnGTC Cuts at: 1689 2547 1689 Size: 858 3125 EaeI y'GGCC_r Cuts at: 1568 1742 2133 2160 3505 1568 Size: 174 391 27 1345 2046 Fragments arranged by size: 2046 1345 391 174 27 EagI C'GGCC_G Cuts at: 1568 1568 Size: 3983 EarI CTCTTCn'nnn_ Cuts at: 887 2006 2216 887 Size: 1119 210 2654 Fragments arranged by size: 2654 1119 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2191 2501 2647 3793 2191 Size: 310 146 1146 2381 Fragments arranged by size: 2381 1146 310 146 EcoICRI GAG'CTC Cuts at: 473 995 473 Size: 522 3461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 587 722 1808 2240 2987 3255 587 Size: 135 1086 432 747 268 1315 Fragments arranged by size: 1315 1086 747 432 268 135 EcoO109I rG'GnC_Cy Cuts at: 113 1122 113 Size: 1009 2974 EcoRI G'AATT_C Cuts at: 987 987 Size: 3983 EcoRV GAT'ATC Cuts at: 698 698 Size: 3983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 928 2002 928 Size: 1074 2909 FspI TGC'GCA Cuts at: 1764 1764 Size: 3983 HaeII r_GCGC'y Cuts at: 1417 1665 2687 3666 1417 Size: 248 1022 979 1734 Fragments arranged by size: 1734 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 161 193 1825 1855 1857 1887 161 Size: 32 1632 30 2 30 2257 Fragments arranged by size: 2257 1632 32 30 30 2 HincII GTy'rAC Cuts at: 1022 1022 Size: 3983 HindIII A'AGCT_T Cuts at: 681 681 Size: 3983 KasI G'GCGC_C Cuts at: 1413 1661 1413 Size: 248 3735 KpnI G_GTAC'C Cuts at: 1003 1003 Size: 3983 MluI A'CGCG_T Cuts at: 3416 3416 Size: 3983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 137 147 885 2654 2838 137 Size: 10 738 1769 184 1282 Fragments arranged by size: 1769 1282 738 184 10 MscI TGG'CCA Cuts at: 1744 1744 Size: 3983 MslI CAynn'nnrTG Cuts at: 760 1972 2099 760 Size: 1212 127 2644 Fragments arranged by size: 2644 1212 127 MspA1I CmG'CkG Cuts at: 1094 1408 1768 2781 3026 1094 Size: 314 360 1013 245 2051 Fragments arranged by size: 2051 1013 360 314 245 NaeI GCC'GGC Cuts at: 2164 2164 Size: 3983 NarI GG'CG_CC Cuts at: 1414 1662 1414 Size: 248 3735 NcoI C'CATG_G Cuts at: 107 441 107 Size: 334 3649 NgoMIV G'CCGG_C Cuts at: 2162 2162 Size: 3983 NspI r_CATG'y Cuts at: 1036 1536 2443 1036 Size: 500 907 2576 Fragments arranged by size: 2576 907 500 PasI CC'CwG_GG Cuts at: 576 576 Size: 3983 PciI A'CATG_T Cuts at: 2439 2439 Size: 3983 PflMI CCAn_nnn'nTGG Cuts at: 442 956 442 Size: 514 3469 PfoI T'CCnGG_A Cuts at: 308 308 Size: 3983 PmeI GTTT'AAAC Cuts at: 982 982 Size: 3983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3122 3154 3122 Size: 32 3951 PpuMI rG'GwC_Cy Cuts at: 113 113 Size: 3983 PspOMI G'GGCC_C Cuts at: 718 718 Size: 3983 PstI C_TGCA'G Cuts at: 1030 1715 1030 Size: 685 3298 PvuI CG_AT'CG Cuts at: 104 104 Size: 3983 PvuII CAG'CTG Cuts at: 1408 1768 1408 Size: 360 3623 RsrII CG'GwC_CG Cuts at: 2178 2178 Size: 3983 SacI G_AGCT'C Cuts at: 475 997 475 Size: 522 3461 SalI G'TCGA_C Cuts at: 1020 1020 Size: 3983 SapI GCTCTTCn'nnn_ Cuts at: 2006 2216 2006 Size: 210 3773 SbfI CC_TGCA'GG Cuts at: 1030 1030 Size: 3983 ScaI AGT'ACT Cuts at: 3500 3500 Size: 3983 SfcI C'TryA_G Cuts at: 33 1026 1711 2704 2895 33 Size: 993 685 993 191 1121 Fragments arranged by size: 1121 993 993 685 191 SfoI GGC'GCC Cuts at: 1415 1663 1415 Size: 248 3735 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3983 SmaI CCC'GGG Cuts at: 1005 1005 Size: 3983 SmlI C'TyrA_G Cuts at: 902 1138 2053 2545 2807 902 Size: 236 915 492 262 2078 Fragments arranged by size: 2078 915 492 262 236 SpeI A'CTAG_T Cuts at: 67 67 Size: 3983 SphI G_CATG'C Cuts at: 1036 1536 1036 Size: 500 3483 StyI C'CwwG_G Cuts at: 107 441 590 1117 1471 107 Size: 334 149 527 354 2619 Fragments arranged by size: 2619 527 354 334 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1566 1699 2158 2367 1566 Size: 133 459 209 3182 Fragments arranged by size: 3182 459 209 133 TatI w'GTAC_w Cuts at: 3498 3498 Size: 3983 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3500 3532 3500 Size: 32 3951 Tth111I GACn'n_nGTC Cuts at: 781 1780 781 Size: 999 2984 XbaI T'CTAG_A Cuts at: 1014 1014 Size: 3983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1478 1478 Size: 3983 XmaI C'CCGG_G Cuts at: 1003 1003 Size: 3983 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI