Restriction map for
pF1KB7974
(Circular) (Six-base) MAPSORT of: pF1KB7974.seq Check: 5050 from: 1 to: 4352
pF1KB7974 4352 bp
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 764 2177 2609 3356 3624 676
Size: 88 1413 432 747 268 1404
Fragments arranged by size:
1413 1404 747 432 268 88
AflIII A'CryG_T
Cuts at: 1704 2808 3785 1704
Size: 1104 977 2271
Fragments arranged by size:
2271 1104 977
AgeI A'CCGG_T
Cuts at: 2740 2740
Size: 4352
AleI CACnn'nnGTG
Cuts at: 1188 1188
Size: 4352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1028 1028
Size: 4352
AlwNI CAG_nnn'CTG
Cuts at: 904 1657 3224 904
Size: 753 1567 2032
Fragments arranged by size:
2032 1567 753
ApaI G_GGCC'C
Cuts at: 585 979 585
Size: 394 3958
ApaLI G'TGCA_C
Cuts at: 3122 3122
Size: 4352
ApoI r'AATT_y
Cuts at: 1356 3705 3961 1356
Size: 2349 256 1747
Fragments arranged by size:
2349 1747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 141 377 1266 1372 141
Size: 236 889 106 3121
Fragments arranged by size:
3121 889 236 106
BamHI G'GATC_C
Cuts at: 1377 1377
Size: 4352
BanI G'GyrC_C
Cuts at: 129 592 606 1368 1782 2030 2065 129
Size: 463 14 762 414 248 35 2416
Fragments arranged by size:
2416 762 463 414 248 35 14
BanII G_rGCy'C
Cuts at: 407 585 712 763 959 979 1118 1201 1366
Size: 178 127 51 196 20 139 83 165
Cuts at: 1366 2396 407
Size: 1030 2363
Fragments arranged by size:
2363 1030 196 178 165 139 127 83
51 20
BbeI G_GCGC'C
Cuts at: 133 1786 2034 133
Size: 1653 248 2451
Fragments arranged by size:
2451 1653 248
BbsI GAAGACnn'nnnn_
Cuts at: 1001 1001
Size: 4352
BbvCI CC'TCA_GC
Cuts at: 842 842
Size: 4352
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3011 4352 2250
Size: 761 1341 2250
Fragments arranged by size:
2250 1341 761
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 580 580
Size: 4352
BglII A'GATC_T
Cuts at: 1867 3904 1867
Size: 2037 2315
BlpI GC'TnA_GC
Cuts at: 1464 1464
Size: 4352
Bme1580I G_kGCm'C
Cuts at: 148 585 611 979 1977 2070 3126 148
Size: 437 26 368 998 93 1056 1374
Fragments arranged by size:
1374 1056 998 437 368 93 26
BmgBI CAC'GTC
Cuts at: 501 829 501
Size: 328 4024
BmrI ACTGGGnnnn_n'
Cuts at: 804 982 1564 1645 1980 4124 804
Size: 178 582 81 335 2144 1032
Fragments arranged by size:
2144 1032 582 335 178 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 2719 583
Size: 2136 2216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 622 739 1231 578
Size: 44 117 492 3699
Fragments arranged by size:
3699 492 117 44
Bpu10I CC'TnA_GC
Cuts at: 842 842
Size: 4352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 391 1528 2407 2899 3197 220
Size: 171 1137 879 492 298 1375
Fragments arranged by size:
1375 1137 879 492 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 628 1265 4056 628
Size: 637 2791 924
Fragments arranged by size:
2791 924 637
BsaAI yAC'GTr
Cuts at: 1705 1705
Size: 4352
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 130 1783 2031 2733 4229 130
Size: 1653 248 702 1496 253
Fragments arranged by size:
1653 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 482 1087 1541 2062 2740 3014 3161 3572 482
Size: 605 454 521 678 274 147 411 1262
Fragments arranged by size:
1262 678 605 521 454 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1207 1237 1207
Size: 30 4322
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 1228 436
Size: 792 3560
BseYI C'CCAG_C
Cuts at: 897 1777 2535 3112 3608 897
Size: 880 758 577 496 1641
Fragments arranged by size:
1641 880 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 523 893 490
Size: 33 370 3949
Fragments arranged by size:
3949 370 33
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3148 104
Size: 1790 46 1208 1308
Fragments arranged by size:
1790 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 712 959 1201 1366 2144 2334 3126 407
Size: 305 247 242 165 778 190 792 1633
Fragments arranged by size:
1633 792 778 305 247 242 190 165
Bsp1286I G_dGCh'C
Cuts at: 148 407 585 611 712 763 959 979 1118
Size: 259 178 26 101 51 196 20 139
Cuts at: 1118 1201 1366 1977 2070 2144 2334 2396 3126
Size: 83 165 611 93 74 190 62 730
Cuts at: 3126 148
Size: 1374
Fragments arranged by size:
1374 730 611 259 196 190 178 165
139 101 93 83 74 62 51 26
20
BspEI T'CCGG_A
Cuts at: 482 1087 3572 482
Size: 605 2485 1262
Fragments arranged by size:
2485 1262 605
BspHI T'CATG_A
Cuts at: 531 3528 531
Size: 2997 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1402 1616 1918 2299 1402
Size: 214 302 381 3455
Fragments arranged by size:
3455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2644 2698 4182 4347 50
Size: 2594 54 1484 165 55
Fragments arranged by size:
2594 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2264 2264
Size: 4352
BsrFI r'CCGG_y
Cuts at: 155 2350 2531 2740 155
Size: 2195 181 209 1767
Fragments arranged by size:
2195 1767 209 181
BssSI C'ACGA_G
Cuts at: 1184 2623 2981 1184
Size: 1439 358 2555
Fragments arranged by size:
2555 1439 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1729 1729
Size: 4352
BstBI TT'CG_AA
Cuts at: 2713 2713
Size: 4352
BstXI CCAn_nnnn'nTGG
Cuts at: 300 300
Size: 4352
BstYI r'GATC_y
Cuts at: 1 1377 1844 1867 2202 2448 3460 3546 3558
Size: 1376 467 23 335 246 1012 86 12
Cuts at: 3558 3904 1
Size: 346 449
Fragments arranged by size:
1376 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 2483 2646 2302
Size: 181 163 4008
Fragments arranged by size:
4008 181 163
BtsI GCAGTG_nn'
Cuts at: 854 876 1000 1595 854
Size: 22 124 595 3611
Fragments arranged by size:
3611 595 124 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 930 965 930
Size: 35 4317
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DraIII CAC_nnn'GTG
Cuts at: 462 462
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 2058 2916 2058
Size: 858 3494
EaeI y'GGCC_r
Cuts at: 572 997 1937 2111 2502 2529 3874 572
Size: 425 940 174 391 27 1345 1050
Fragments arranged by size:
1345 1050 940 425 391 174 27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 652 2375 2585 652
Size: 1723 210 2419
Fragments arranged by size:
2419 1723 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 291 2560 2870 3016 4162 127
Size: 164 2269 310 146 1146 317
Fragments arranged by size:
2269 1146 317 310 164 146
EcoICRI GAG'CTC
Cuts at: 405 710 957 1199 1364 405
Size: 305 247 242 165 3393
Fragments arranged by size:
3393 305 247 242 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 622 676 739 764 1231 2177 2609 3356
Size: 44 54 63 25 467 946 432 747
Cuts at: 3356 3624 578
Size: 268 1306
Fragments arranged by size:
1306 946 747 467 432 268 63 54
44 25
EcoNI CCTnn'n_nnAGG
Cuts at: 205 205
Size: 4352
EcoO109I rG'GnC_Cy
Cuts at: 361 582 1491 361
Size: 221 909 3222
Fragments arranged by size:
3222 909 221
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 133 1786 2034 3056 4035 133
Size: 1653 248 1022 979 450
Fragments arranged by size:
1653 1022 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1207 1239 2194 2224 2226 2256 313
Size: 32 862 32 955 30 2 30 2409
Fragments arranged by size:
2409 955 862 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1391 1391
Size: 4352
KasI G'GCGC_C
Cuts at: 129 1782 2030 129
Size: 1653 248 2451
Fragments arranged by size:
2451 1653 248
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3207 3023
Size: 184 4168
MscI TGG'CCA
Cuts at: 574 2113 574
Size: 1539 2813
MslI CAynn'nnrTG
Cuts at: 1188 2341 2468 1188
Size: 1153 127 3072
Fragments arranged by size:
3072 1153 127
MspA1I CmG'CkG
Cuts at: 247 435 512 904 1463 1777 2137 3150 3395
Size: 188 77 392 559 314 360 1013 245
Cuts at: 3395 247
Size: 1204
Fragments arranged by size:
1204 1013 559 392 360 314 245 188
77
NaeI GCC'GGC
Cuts at: 157 2533 157
Size: 2376 1976
NarI GG'CG_CC
Cuts at: 130 1783 2031 130
Size: 1653 248 2451
Fragments arranged by size:
2451 1653 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4352
NgoMIV G'CCGG_C
Cuts at: 155 2531 155
Size: 2376 1976
NspI r_CATG'y
Cuts at: 1405 1905 2812 1405
Size: 500 907 2945
Fragments arranged by size:
2945 907 500
PasI CC'CwG_GG
Cuts at: 928 928
Size: 4352
PciI A'CATG_T
Cuts at: 2808 2808
Size: 4352
PflMI CCAn_nnn'nTGG
Cuts at: 888 888
Size: 4352
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 4320
PspOMI G'GGCC_C
Cuts at: 581 975 581
Size: 394 3958
PstI C_TGCA'G
Cuts at: 209 431 547 1007 1399 2084 209
Size: 222 116 460 392 685 2477
Fragments arranged by size:
2477 685 460 392 222 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 247 512 904 1777 2137 247
Size: 265 392 873 360 2462
Fragments arranged by size:
2462 873 392 360 265
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 407 712 959 1201 1366 407
Size: 305 247 242 165 3393
Fragments arranged by size:
3393 305 247 242 165
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SfcI C'TryA_G
Cuts at: 33 205 427 543 1003 1395 2080 3073 3264
Size: 172 222 116 460 392 685 993 191
Cuts at: 3264 33
Size: 1121
Fragments arranged by size:
1121 993 685 460 392 222 191 172
116
SfiI GGCCn_nnn'nGGCC
Cuts at: 580 580
Size: 4352
SfoI GGC'GCC
Cuts at: 131 1784 2032 131
Size: 1653 248 2451
Fragments arranged by size:
2451 1653 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 143 379 1268 1374 143
Size: 236 889 106 3121
Fragments arranged by size:
3121 889 236 106
SmlI C'TyrA_G
Cuts at: 235 406 1507 2422 2914 3176 235
Size: 171 1101 915 492 262 1411
Fragments arranged by size:
1411 1101 915 492 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StyI C'CwwG_G
Cuts at: 107 215 1486 1840 107
Size: 108 1271 354 2619
Fragments arranged by size:
2619 1271 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 456 1935 2068 2527 2736 456
Size: 1479 133 459 209 2072
Fragments arranged by size:
2072 1479 459 209 133
TatI w'GTAC_w
Cuts at: 3867 3867
Size: 4352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 889 1140 1847 889
Size: 251 707 3394
Fragments arranged by size:
3394 707 251
XmaI C'CCGG_G
Cuts at: 141 377 1266 1372 141
Size: 236 889 106 3121
Fragments arranged by size:
3121 889 236 106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI