Restriction map for
pF1KB7975
(Circular) (Six-base) MAPSORT of: pF1KB7975.seq Check: 4437 from: 1 to: 4394
pF1KB7975 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1315 1315
Size: 4394
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 676 1287 2219 2651 3398 3666 601
Size: 75 611 932 432 747 268 1329
Fragments arranged by size:
1329 932 747 611 432 268 75
AfeI AGC'GCT
Cuts at: 529 1144 529
Size: 615 3779
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AhdI GACnn_n'nnGTC
Cuts at: 246 456 246
Size: 210 4184
AlwNI CAG_nnn'CTG
Cuts at: 884 1278 1699 3266 884
Size: 394 421 1567 2012
Fragments arranged by size:
2012 1567 421 394
ApaLI G'TGCA_C
Cuts at: 1120 3164 1120
Size: 2044 2350
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
BamHI G'GATC_C
Cuts at: 873 1419 873
Size: 546 3848
BanI G'GyrC_C
Cuts at: 1089 1243 1336 1344 1410 1824 2072 2107 1089
Size: 154 93 8 66 414 248 35 3376
Fragments arranged by size:
3376 414 248 154 93 66 35 8
BanII G_rGCy'C
Cuts at: 1408 2438 1408
Size: 1030 3364
BbeI G_GCGC'C
Cuts at: 1093 1340 1828 2076 1093
Size: 247 488 248 3411
Fragments arranged by size:
3411 488 248 247
BciVI GTATCCnnnnn_n'
Cuts at: 585 2292 3053 4394 585
Size: 1707 761 1341 585
Fragments arranged by size:
1707 1341 761 585
BclI T'GATC_A
Cuts at: 950 1914 950
Size: 964 3430
BfrBI ATG'CAT
Cuts at: 981 981
Size: 4394
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1279 1506 1279
Size: 227 4167
Bme1580I G_kGCm'C
Cuts at: 832 1066 1085 1124 1349 2019 2112 3168 832
Size: 234 19 39 225 670 93 1056 2058
Fragments arranged by size:
2058 1056 670 234 225 93 39 19
BmgBI CAC'GTC
Cuts at: 1080 1080
Size: 4394
BmrI ACTGGGnnnn_n'
Cuts at: 872 1188 1266 1343 1606 1687 2022 4166 872
Size: 316 78 77 263 81 335 2144 1100
Fragments arranged by size:
2144 1100 335 316 263 81 78 77
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 919 958 1069 1132 100
Size: 819 39 111 63 3362
Fragments arranged by size:
3362 819 111 63 39
Bpu10I CC'TnA_GC
Cuts at: 778 778
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1570 2449 2941 3239 298
Size: 1272 879 492 298 1453
Fragments arranged by size:
1453 1272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 4098 760
Size: 3338 1056
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1090 1337 1825 2073 2775 4271 1090
Size: 247 488 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 488 248 247
BsaWI w'CCGG_w
Cuts at: 242 470 989 1583 2104 2782 3056 3203 3614
Size: 228 519 594 521 678 274 147 411
Cuts at: 3614 242
Size: 1022
Fragments arranged by size:
1022 678 594 521 519 411 274 228
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1246 1276 1246
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 1231 346
Size: 885 3509
BseYI C'CCAG_C
Cuts at: 1058 1819 2577 3154 3650 1058
Size: 761 758 577 496 1802
Fragments arranged by size:
1802 761 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 261 276 1936 1982 3190 104
Size: 157 15 1660 46 1208 1308
Fragments arranged by size:
1660 1308 1208 157 46 15
BsiHKAI G_wGCw'C
Cuts at: 1124 1223 1285 1408 2186 2376 3168 1124
Size: 99 62 123 778 190 792 2350
Fragments arranged by size:
2350 792 778 190 123 99 62
BsmBI CGTCTCn'nnnn_
Cuts at: 781 781
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 832 1066 1085 1124 1223 1285 1349 1408 2019
Size: 234 19 39 99 62 64 59 611
Cuts at: 2019 2112 2186 2376 2438 3168 832
Size: 93 74 190 62 730 2058
Fragments arranged by size:
2058 730 611 234 190 99 93 74
64 62 62 59 39 19
BspEI T'CCGG_A
Cuts at: 989 3614 989
Size: 2625 1769
BspHI T'CATG_A
Cuts at: 974 3570 974
Size: 2596 1798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 725 789 1315 1444 1658 1960 2341 725
Size: 64 526 129 214 302 381 2778
Fragments arranged by size:
2778 526 381 302 214 129 64
BsrBI CCG'CTC
Cuts at: 50 320 336 1251 1315 2686 2740 4224 4389
Size: 270 16 915 64 1371 54 1484 165
Cuts at: 4389 50
Size: 55
Fragments arranged by size:
1484 1371 915 270 165 64 55 54
16
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 562 2392 2573 2782 562
Size: 1830 181 209 2174
Fragments arranged by size:
2174 1830 209 181
BssHII G'CGCG_C
Cuts at: 125 125
Size: 4394
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 1147 1356 1147
Size: 209 4185
BstYI r'GATC_y
Cuts at: 1 873 986 1419 1886 1909 2244 2490 3502
Size: 872 113 433 467 23 335 246 1012
Cuts at: 3502 3588 3600 3946 1
Size: 86 12 346 449
Fragments arranged by size:
1012 872 467 449 433 346 335 246
113 86 23 12
BtgI C'CryG_G
Cuts at: 107 399 1265 107
Size: 292 866 3236
Fragments arranged by size:
3236 866 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1371 1637 1371
Size: 266 4128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 303 338 303
Size: 35 4359
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 194 258 564 1013 1979 2153 2544 2571 3916
Size: 64 306 449 966 174 391 27 1345
Cuts at: 3916 194
Size: 672
Fragments arranged by size:
1345 966 672 449 391 306 174 64
27
EagI C'GGCC_G
Cuts at: 258 1979 258
Size: 1721 2673
EarI CTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 450 2602 2912 3058 4204 450
Size: 2152 310 146 1146 640
Fragments arranged by size:
2152 1146 640 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 601 676 919 958 1069 1132 1287 2219
Size: 501 75 243 39 111 63 155 932
Cuts at: 2219 2651 3398 3666 100
Size: 432 747 268 828
Fragments arranged by size:
932 828 747 501 432 268 243 155
111 75 63 39
EcoO109I rG'GnC_Cy
Cuts at: 248 422 704 933 1533 248
Size: 174 282 229 600 3109
Fragments arranged by size:
3109 600 282 229 174
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 740 2175 740
Size: 1435 2959
HaeII r_GCGC'y
Cuts at: 531 785 1093 1146 1340 1828 2076 3098 4077
Size: 254 308 53 194 488 248 1022 979
Cuts at: 4077 531
Size: 848
Fragments arranged by size:
1022 979 848 488 308 254 248 194
53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 442 474 1246 1278 2236 2266 2268
Size: 32 178 32 772 32 958 30 2
Cuts at: 2268 2298 232
Size: 30 2328
Fragments arranged by size:
2328 958 772 178 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 804 1433 804
Size: 629 3765
KasI G'GCGC_C
Cuts at: 1089 1336 1824 2072 1089
Size: 247 488 248 3411
Fragments arranged by size:
3411 488 248 247
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MfeI C'AATT_G
Cuts at: 440 440
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 1015 2155 1015
Size: 1140 3254
MslI CAynn'nnrTG
Cuts at: 2383 2510 2383
Size: 127 4267
MspA1I CmG'CkG
Cuts at: 257 1058 1173 1505 1819 2179 3192 3437 257
Size: 801 115 332 314 360 1013 245 1214
Fragments arranged by size:
1214 1013 801 360 332 314 245 115
NaeI GCC'GGC
Cuts at: 564 2575 564
Size: 2011 2383
NarI GG'CG_CC
Cuts at: 1090 1337 1825 2073 1090
Size: 247 488 248 3411
Fragments arranged by size:
3411 488 248 247
NcoI C'CATG_G
Cuts at: 107 1265 107
Size: 1158 3236
NgoMIV G'CCGG_C
Cuts at: 562 2573 562
Size: 2011 2383
NsiI A_TGCA'T
Cuts at: 983 983
Size: 4394
NspI r_CATG'y
Cuts at: 233 408 1447 1947 2854 233
Size: 175 1039 500 907 1773
Fragments arranged by size:
1773 1039 907 500 175
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PfoI T'CCnGG_A
Cuts at: 896 1109 896
Size: 213 4181
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 248 422 704 248
Size: 174 282 3938
Fragments arranged by size:
3938 282 174
PstI C_TGCA'G
Cuts at: 256 797 1441 2126 256
Size: 541 644 685 2524
Fragments arranged by size:
2524 685 644 541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1058 1819 2179 1058
Size: 761 360 3273
Fragments arranged by size:
3273 761 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 252 793 1437 2122 3115 3306 33
Size: 219 541 644 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 541 219 191
SfoI GGC'GCC
Cuts at: 1091 1338 1826 2074 1091
Size: 247 488 248 3411
Fragments arranged by size:
3411 488 248 247
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 313 1549 2464 2956 3218 313
Size: 1236 915 492 262 1489
Fragments arranged by size:
1489 1236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 233 408 1447 1947 233
Size: 175 1039 500 2680
Fragments arranged by size:
2680 1039 500 175
StyI C'CwwG_G
Cuts at: 107 1085 1265 1340 1528 1882 107
Size: 978 180 75 188 354 2619
Fragments arranged by size:
2619 978 354 188 180 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 905 1133 3909 905
Size: 228 2776 1390
Fragments arranged by size:
2776 1390 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 170 277 2191 170
Size: 107 1914 2373
Fragments arranged by size:
2373 1914 107
XbaI T'CTAG_A
Cuts at: 606 1425 606
Size: 819 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmtI BsgI BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI