Restriction map for
pF1KB7978
(Circular) (Six-base) MAPSORT of: pF1KB7978.seq Check: 2931 from: 1 to: 4400
pF1KB7978 4400 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1438 1438
Size: 4400
Acc65I G'GTAC_C
Cuts at: 1416 1416
Size: 4400
AclI AA'CG_TT
Cuts at: 4240 4240
Size: 4400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 2225 2657 3404 3672 807
Size: 1418 432 747 268 1535
Fragments arranged by size:
1535 1418 747 432 268
AfeI AGC'GCT
Cuts at: 1251 1251
Size: 4400
AflIII A'CryG_T
Cuts at: 1752 2856 3833 1752
Size: 1104 977 2319
Fragments arranged by size:
2319 1104 977
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AleI CACnn'nnGTG
Cuts at: 634 634
Size: 4400
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 859 891 859
Size: 32 4368
AlwNI CAG_nnn'CTG
Cuts at: 708 1705 3272 708
Size: 997 1567 1836
Fragments arranged by size:
1836 1567 997
ApaI G_GGCC'C
Cuts at: 281 1095 1115 281
Size: 814 20 3566
Fragments arranged by size:
3566 814 20
ApaLI G'TGCA_C
Cuts at: 3170 3170
Size: 4400
ApoI r'AATT_y
Cuts at: 1404 3753 4009 1404
Size: 2349 256 1795
Fragments arranged by size:
2349 1795 256
AscI GG'CGCG_CC
Cuts at: 288 288
Size: 4400
AseI AT'TA_AT
Cuts at: 20 20
Size: 4400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 298 680 794 961 1002 1086 1420 298
Size: 382 114 167 41 84 334 3278
Fragments arranged by size:
3278 382 334 167 114 84 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 452 485 452
Size: 33 4367
BamHI G'GATC_C
Cuts at: 1425 1425
Size: 4400
BanI G'GyrC_C
Cuts at: 695 853 1416 1830 2078 2113 695
Size: 158 563 414 248 35 2982
Fragments arranged by size:
2982 563 414 248 158 35
BanII G_rGCy'C
Cuts at: 281 347 689 1095 1115 1414 2444 281
Size: 66 342 406 20 299 1030 2237
Fragments arranged by size:
2237 1030 406 342 299 66 20
BbeI G_GCGC'C
Cuts at: 1834 2082 1834
Size: 248 4152
BbvCI CC'TCA_GC
Cuts at: 1024 1024
Size: 4400
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 1920 1920
Size: 4400
BfrBI ATG'CAT
Cuts at: 954 954
Size: 4400
BglI GCCn_nnn'nGGC
Cuts at: 285 693 797 1295 285
Size: 408 104 498 3390
Fragments arranged by size:
3390 498 408 104
BglII A'GATC_T
Cuts at: 1915 3952 1915
Size: 2037 2363
BlpI GC'TnA_GC
Cuts at: 256 506 1512 256
Size: 250 1006 3144
Fragments arranged by size:
3144 1006 250
Bme1580I G_kGCm'C
Cuts at: 281 1009 1095 1115 2025 2118 3174 281
Size: 728 86 20 910 93 1056 1507
Fragments arranged by size:
1507 1056 910 728 93 86 20
BmgBI CAC'GTC
Cuts at: 321 450 321
Size: 129 4271
BmrI ACTGGGnnnn_n'
Cuts at: 1612 1693 2028 4172 1612
Size: 81 335 2144 1840
Fragments arranged by size:
2144 1840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 324 516 820 2767 324
Size: 192 304 1947 1957
Fragments arranged by size:
1957 1947 304 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4400
Bpu10I CC'TnA_GC
Cuts at: 1024 1351 1024
Size: 327 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1576 2455 2947 3245 1576
Size: 879 492 298 2731
Fragments arranged by size:
2731 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4104 4104
Size: 4400
BsaAI yAC'GTr
Cuts at: 1753 1753
Size: 4400
BsaBI GATnn'nnATC
Cuts at: 6 1914 3951 6
Size: 1908 2037 455
Fragments arranged by size:
2037 1908 455
BsaHI Gr'CG_yC
Cuts at: 1831 2079 2781 4277 1831
Size: 248 702 1496 1954
Fragments arranged by size:
1954 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1589 2110 2788 3062 3209 3620 1589
Size: 521 678 274 147 411 2369
Fragments arranged by size:
2369 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 859 889 1162 1192 859
Size: 30 273 30 4067
Fragments arranged by size:
4067 273 30 30
BseYI C'CCAG_C
Cuts at: 787 1285 1825 2583 3160 3656 787
Size: 498 540 758 577 496 1531
Fragments arranged by size:
1531 758 577 540 498 496
BsiEI CG_ry'CG
Cuts at: 104 272 527 1212 1942 1988 3196 104
Size: 168 255 685 730 46 1208 1308
Fragments arranged by size:
1308 1208 730 685 255 168 46
BsiHKAI G_wGCw'C
Cuts at: 347 784 1357 1414 2192 2382 3174 347
Size: 437 573 57 778 190 792 1573
Fragments arranged by size:
1573 792 778 573 437 190 57
Bsp1286I G_dGCh'C
Cuts at: 281 347 689 784 1009 1095 1115 1357 1414
Size: 66 342 95 225 86 20 242 57
Cuts at: 1414 2025 2118 2192 2382 2444 3174 281
Size: 611 93 74 190 62 730 1507
Fragments arranged by size:
1507 730 611 342 242 225 190 95
93 86 74 66 62 57 20
BspEI T'CCGG_A
Cuts at: 3620 3620
Size: 4400
BspHI T'CATG_A
Cuts at: 530 1277 3576 530
Size: 747 2299 1354
Fragments arranged by size:
2299 1354 747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1450 1664 1966 2347 1450
Size: 214 302 381 3503
Fragments arranged by size:
3503 381 302 214
BsrBI CCG'CTC
Cuts at: 50 899 1109 1214 2692 2746 4230 4395 50
Size: 849 210 105 1478 54 1484 165 55
Fragments arranged by size:
1484 1478 849 210 165 105 55 54
BsrDI GCAATG_nn'
Cuts at: 492 522 2312 492
Size: 30 1790 2580
Fragments arranged by size:
2580 1790 30
BsrFI r'CCGG_y
Cuts at: 698 1041 1189 1267 2398 2579 2788 698
Size: 343 148 78 1131 181 209 2310
Fragments arranged by size:
2310 1131 343 209 181 148 78
BssHII G'CGCG_C
Cuts at: 251 260 288 251
Size: 9 28 4363
Fragments arranged by size:
4363 28 9
BssSI C'ACGA_G
Cuts at: 346 1008 2671 3029 346
Size: 662 1663 358 1717
Fragments arranged by size:
1717 1663 662 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1777 1777
Size: 4400
BstBI TT'CG_AA
Cuts at: 2761 2761
Size: 4400
BstEII G'GTnAC_C
Cuts at: 663 663
Size: 4400
BstXI CCAn_nnnn'nTGG
Cuts at: 636 1306 636
Size: 670 3730
BstYI r'GATC_y
Cuts at: 1 1425 1892 1915 2250 2496 3508 3594 3606
Size: 1424 467 23 335 246 1012 86 12
Cuts at: 3606 3952 1
Size: 346 449
Fragments arranged by size:
1424 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 272 668 1181 1343 107
Size: 165 396 513 162 3164
Fragments arranged by size:
3164 513 396 165 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2350 2531 2694 2350
Size: 181 163 4056
Fragments arranged by size:
4056 181 163
BtsI GCAGTG_nn'
Cuts at: 585 1206 1643 585
Size: 621 437 3342
Fragments arranged by size:
3342 621 437
DraI TTT'AAA
Cuts at: 1399 1399
Size: 4400
DrdI GACnn_nn'nnGTC
Cuts at: 2106 2964 2106
Size: 858 3542
EaeI y'GGCC_r
Cuts at: 269 524 545 1043 1187 1296 1985 2159 2550
Size: 255 21 498 144 109 689 174 391
Cuts at: 2550 2577 3922 269
Size: 27 1345 747
Fragments arranged by size:
1345 747 689 498 391 255 174 144
109 27 21
EagI C'GGCC_G
Cuts at: 269 524 1985 269
Size: 255 1461 2684
Fragments arranged by size:
2684 1461 255
EarI CTCTTCn'nnn_
Cuts at: 586 2423 2633 586
Size: 1837 210 2353
Fragments arranged by size:
2353 1837 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 343 2608 2918 3064 4210 343
Size: 2265 310 146 1146 533
Fragments arranged by size:
2265 1146 533 310 146
EcoICRI GAG'CTC
Cuts at: 345 1412 345
Size: 1067 3333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 807 2225 2657 3404 3672 730
Size: 77 1418 432 747 268 1458
Fragments arranged by size:
1458 1418 747 432 268 77
EcoO109I rG'GnC_Cy
Cuts at: 277 747 1091 1092 1112 1539 277
Size: 470 344 1 20 427 3138
Fragments arranged by size:
3138 470 427 344 20 1
EcoRI G'AATT_C
Cuts at: 1404 1404
Size: 4400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4400
FspI TGC'GCA
Cuts at: 352 514 763 2181 352
Size: 162 249 1418 2571
Fragments arranged by size:
2571 1418 249 162
FspAI rTGC'GCAy
Cuts at: 352 352
Size: 4400
HaeII r_GCGC'y
Cuts at: 402 995 1253 1834 2082 3104 4083 402
Size: 593 258 581 248 1022 979 719
Fragments arranged by size:
1022 979 719 593 581 258 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 942 974 2242 2272 2274 2304 942
Size: 32 1268 30 2 30 3038
Fragments arranged by size:
3038 1268 32 30 30 2
HincII GTy'rAC
Cuts at: 1439 1439
Size: 4400
KasI G'GCGC_C
Cuts at: 1830 2078 1830
Size: 248 4152
KpnI G_GTAC'C
Cuts at: 1420 1420
Size: 4400
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 902 3071 3255 902
Size: 2169 184 2047
Fragments arranged by size:
2169 2047 184
MscI TGG'CCA
Cuts at: 547 2161 547
Size: 1614 2786
MslI CAynn'nnrTG
Cuts at: 613 634 661 769 2389 2516 613
Size: 21 27 108 1620 127 2497
Fragments arranged by size:
2497 1620 127 108 27 21
MspA1I CmG'CkG
Cuts at: 248 505 976 1511 1825 2185 3198 3443 248
Size: 257 471 535 314 360 1013 245 1205
Fragments arranged by size:
1205 1013 535 471 360 314 257 245
NaeI GCC'GGC
Cuts at: 1043 1191 1269 2581 1043
Size: 148 78 1312 2862
Fragments arranged by size:
2862 1312 148 78
NarI GG'CG_CC
Cuts at: 1831 2079 1831
Size: 248 4152
NcoI C'CATG_G
Cuts at: 107 1181 107
Size: 1074 3326
NgoMIV G'CCGG_C
Cuts at: 1041 1189 1267 2579 1041
Size: 148 78 1312 2862
Fragments arranged by size:
2862 1312 148 78
NsiI A_TGCA'T
Cuts at: 956 956
Size: 4400
NspI r_CATG'y
Cuts at: 768 1041 1230 1453 1953 2860 768
Size: 273 189 223 500 907 2308
Fragments arranged by size:
2308 907 500 273 223 189
PciI A'CATG_T
Cuts at: 2856 2856
Size: 4400
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 859 891 3539 3571 859
Size: 32 2648 32 1688
Fragments arranged by size:
2648 1688 32 32
PpuMI rG'GwC_Cy
Cuts at: 747 747
Size: 4400
PspOMI G'GGCC_C
Cuts at: 277 1091 1111 277
Size: 814 20 3566
Fragments arranged by size:
3566 814 20
PstI C_TGCA'G
Cuts at: 1447 2132 1447
Size: 685 3715
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 976 1825 2185 976
Size: 849 360 3191
Fragments arranged by size:
3191 849 360
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 347 1414 347
Size: 1067 3333
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
SbfI CC_TGCA'GG
Cuts at: 1447 1447
Size: 4400
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SexAI A'CCwGG_T
Cuts at: 539 539
Size: 4400
SfcI C'TryA_G
Cuts at: 33 987 1443 2128 3121 3312 33
Size: 954 456 685 993 191 1121
Fragments arranged by size:
1121 993 954 685 456 191
SfoI GGC'GCC
Cuts at: 1832 2080 1832
Size: 248 4152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 682 963 1088 1422 682
Size: 281 125 334 3660
Fragments arranged by size:
3660 334 281 125
SmlI C'TyrA_G
Cuts at: 1555 2470 2962 3224 1555
Size: 915 492 262 2731
Fragments arranged by size:
2731 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 768 1041 1230 1453 1953 768
Size: 273 189 223 500 3215
Fragments arranged by size:
3215 500 273 223 189
StyI C'CwwG_G
Cuts at: 107 849 1181 1534 1888 107
Size: 742 332 353 354 2619
Fragments arranged by size:
2619 742 354 353 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 646 801 1356 1983 2116 2575 2784 646
Size: 155 555 627 133 459 209 2262
Fragments arranged by size:
2262 627 555 459 209 155 133
TatI w'GTAC_w
Cuts at: 3915 3915
Size: 4400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4368
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 1431 1431
Size: 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1895 1895
Size: 4400
XmaI C'CCGG_G
Cuts at: 680 961 1086 1420 680
Size: 281 125 334 3660
Fragments arranged by size:
3660 334 281 125
XmnI GAAnn'nnTTC
Cuts at: 595 871 595
Size: 276 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AvrII BbsI BcgI
BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI