(Circular) (Six-base) MAPSORT of: pF1KB7985.seq Check: 8163 from: 1 to: 4406 pF1KB7985 4406 bp With 182 enzymes: SgfI * March 2, 2010 14:28 .. AccI GT'mk_AC Cuts at: 1444 1444 Size: 4406 Acc65I G'GTAC_C Cuts at: 1422 1422 Size: 4406 AclI AA'CG_TT Cuts at: 944 4246 944 Size: 3302 1104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 2663 3410 3678 2231 Size: 432 747 268 2959 Fragments arranged by size: 2959 747 432 268 AflIII A'CryG_T Cuts at: 824 1007 1758 2862 3839 824 Size: 183 751 1104 977 1391 Fragments arranged by size: 1391 1104 977 751 183 AgeI A'CCGG_T Cuts at: 2794 2794 Size: 4406 AleI CACnn'nnGTG Cuts at: 813 813 Size: 4406 AlwNI CAG_nnn'CTG Cuts at: 1042 1711 3278 1042 Size: 669 1567 2170 Fragments arranged by size: 2170 1567 669 ApaLI G'TGCA_C Cuts at: 3176 3176 Size: 4406 ApoI r'AATT_y Cuts at: 1021 1045 1410 3759 4015 1021 Size: 24 365 2349 256 1412 Fragments arranged by size: 2349 1412 365 256 24 AseI AT'TA_AT Cuts at: 20 20 Size: 4406 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4406 AvaI C'yCGr_G Cuts at: 582 811 1426 582 Size: 229 615 3562 Fragments arranged by size: 3562 615 229 BamHI G'GATC_C Cuts at: 453 1431 453 Size: 978 3428 BanI G'GyrC_C Cuts at: 148 1422 1836 2084 2119 148 Size: 1274 414 248 35 2435 Fragments arranged by size: 2435 1274 414 248 35 BanII G_rGCy'C Cuts at: 410 1199 1420 2450 410 Size: 789 221 1030 2366 Fragments arranged by size: 2366 1030 789 221 BbeI G_GCGC'C Cuts at: 1840 2088 1840 Size: 248 4158 BbsI GAAGACnn'nnnn_ Cuts at: 584 711 584 Size: 127 4279 BbvCI CC'TCA_GC Cuts at: 751 1330 751 Size: 579 3827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1009 1043 1009 Size: 34 4372 BciVI GTATCCnnnnn_n' Cuts at: 2304 3065 4406 2304 Size: 761 1341 2304 Fragments arranged by size: 2304 1341 761 BclI T'GATC_A Cuts at: 1926 1926 Size: 4406 BglI GCCn_nnn'nGGC Cuts at: 1309 1309 Size: 4406 BglII A'GATC_T Cuts at: 877 1921 3958 877 Size: 1044 2037 1325 Fragments arranged by size: 2037 1325 1044 BlpI GC'TnA_GC Cuts at: 508 1518 508 Size: 1010 3396 Bme1580I G_kGCm'C Cuts at: 187 2031 2124 3180 187 Size: 1844 93 1056 1413 Fragments arranged by size: 1844 1413 1056 93 BmgBI CAC'GTC Cuts at: 315 315 Size: 4406 BmrI ACTGGGnnnn_n' Cuts at: 285 1618 1699 2034 4178 285 Size: 1333 81 335 2144 513 Fragments arranged by size: 2144 1333 513 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2773 2773 Size: 4406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1213 1213 Size: 4406 Bpu10I CC'TnA_GC Cuts at: 513 751 1148 1330 513 Size: 238 397 182 3589 Fragments arranged by size: 3589 397 238 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1291 1582 2461 2953 3251 1291 Size: 291 879 492 298 2446 Fragments arranged by size: 2446 879 492 298 291 BsaI GGTCTCn'nnnn_ Cuts at: 518 4110 518 Size: 3592 814 BsaAI yAC'GTr Cuts at: 1008 1759 1008 Size: 751 3655 BsaBI GATnn'nnATC Cuts at: 6 1920 3957 6 Size: 1914 2037 455 Fragments arranged by size: 2037 1914 455 BsaHI Gr'CG_yC Cuts at: 1837 2085 2787 4283 1837 Size: 248 702 1496 1960 Fragments arranged by size: 1960 1496 702 248 BsaWI w'CCGG_w Cuts at: 848 1595 2116 2794 3068 3215 3626 848 Size: 747 521 678 274 147 411 1628 Fragments arranged by size: 1628 747 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 965 995 1219 1249 965 Size: 30 224 30 4122 Fragments arranged by size: 4122 224 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 574 739 1399 574 Size: 165 660 3581 Fragments arranged by size: 3581 660 165 BseYI C'CCAG_C Cuts at: 176 180 350 834 1831 2589 3166 3662 176 Size: 4 170 484 997 758 577 496 920 Fragments arranged by size: 997 920 758 577 496 484 170 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 328 397 475 811 328 Size: 69 78 336 3923 Fragments arranged by size: 3923 336 78 69 BsiEI CG_ry'CG Cuts at: 104 1948 1994 3202 104 Size: 1844 46 1208 1308 Fragments arranged by size: 1844 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 848 1420 2198 2388 3180 848 Size: 572 778 190 792 2074 Fragments arranged by size: 2074 792 778 572 190 BsmBI CGTCTCn'nnnn_ Cuts at: 663 921 663 Size: 258 4148 Bsp1286I G_dGCh'C Cuts at: 187 410 848 1199 1420 2031 2124 2198 2388 Size: 223 438 351 221 611 93 74 190 Cuts at: 2388 2450 3180 187 Size: 62 730 1413 Fragments arranged by size: 1413 730 611 438 351 223 221 190 93 74 62 BspEI T'CCGG_A Cuts at: 848 3626 848 Size: 2778 1628 BspHI T'CATG_A Cuts at: 3582 3582 Size: 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1148 1456 1670 1972 2353 1148 Size: 308 214 302 381 3201 Fragments arranged by size: 3201 381 308 302 214 BsrBI CCG'CTC Cuts at: 50 2698 2752 4236 4401 50 Size: 2648 54 1484 165 55 Fragments arranged by size: 2648 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 657 2318 657 Size: 1661 2745 BsrFI r'CCGG_y Cuts at: 2404 2585 2794 2404 Size: 181 209 4016 Fragments arranged by size: 4016 209 181 BssSI C'ACGA_G Cuts at: 207 478 2677 3035 207 Size: 271 2199 358 1578 Fragments arranged by size: 2199 1578 358 271 BstAPI GCAn_nnn'nTGC Cuts at: 1783 1783 Size: 4406 BstBI TT'CG_AA Cuts at: 2767 2767 Size: 4406 BstXI CCAn_nnnn'nTGG Cuts at: 403 403 Size: 4406 BstYI r'GATC_y Cuts at: 1 300 453 877 1431 1898 1921 2256 2502 Size: 299 153 424 554 467 23 335 246 Cuts at: 2502 3514 3600 3612 3958 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 554 467 449 424 346 335 299 246 153 86 23 12 BtgI C'CryG_G Cuts at: 131 1239 1256 1268 1313 131 Size: 1108 17 12 45 3224 Fragments arranged by size: 3224 1108 45 17 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2356 2537 2700 2356 Size: 181 163 4062 Fragments arranged by size: 4062 181 163 BtsI GCAGTG_nn' Cuts at: 1380 1649 1380 Size: 269 4137 DraI TTT'AAA Cuts at: 1405 1405 Size: 4406 DrdI GACnn_nn'nnGTC Cuts at: 2112 2970 2112 Size: 858 3548 EaeI y'GGCC_r Cuts at: 381 1991 2165 2556 2583 3928 381 Size: 1610 174 391 27 1345 859 Fragments arranged by size: 1610 1345 859 391 174 27 EagI C'GGCC_G Cuts at: 1991 1991 Size: 4406 EarI CTCTTCn'nnn_ Cuts at: 694 2429 2639 694 Size: 1735 210 2461 Fragments arranged by size: 2461 1735 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 651 799 854 2614 2924 3070 4216 651 Size: 148 55 1760 310 146 1146 841 Fragments arranged by size: 1760 1146 841 310 148 146 55 EcoICRI GAG'CTC Cuts at: 1418 1418 Size: 4406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1213 2231 2663 3410 3678 1213 Size: 1018 432 747 268 1941 Fragments arranged by size: 1941 1018 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 483 564 622 1302 1545 483 Size: 81 58 680 243 3344 Fragments arranged by size: 3344 680 243 81 58 EcoRI G'AATT_C Cuts at: 1410 1410 Size: 4406 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 4406 FspI TGC'GCA Cuts at: 2187 2187 Size: 4406 HaeII r_GCGC'y Cuts at: 215 1840 2088 3110 4089 215 Size: 1625 248 1022 979 532 Fragments arranged by size: 1625 1022 979 532 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 292 324 965 997 2248 2278 2280 2310 292 Size: 32 641 32 1251 30 2 30 2388 Fragments arranged by size: 2388 1251 641 32 32 30 30 2 HincII GTy'rAC Cuts at: 318 1445 318 Size: 1127 3279 KasI G'GCGC_C Cuts at: 1836 2084 1836 Size: 248 4158 KpnI G_GTAC'C Cuts at: 1426 1426 Size: 4406 MluI A'CGCG_T Cuts at: 824 3839 824 Size: 3015 1391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 668 3077 3261 668 Size: 2409 184 1813 Fragments arranged by size: 2409 1813 184 MscI TGG'CCA Cuts at: 2167 2167 Size: 4406 MslI CAynn'nnrTG Cuts at: 813 2395 2522 813 Size: 1582 127 2697 Fragments arranged by size: 2697 1582 127 MspA1I CmG'CkG Cuts at: 180 222 441 795 1258 1270 1517 1831 2191 Size: 42 219 354 463 12 247 314 360 Cuts at: 2191 3204 3449 180 Size: 1013 245 1137 Fragments arranged by size: 1137 1013 463 360 354 314 247 245 219 42 12 NaeI GCC'GGC Cuts at: 2587 2587 Size: 4406 NarI GG'CG_CC Cuts at: 1837 2085 1837 Size: 248 4158 NcoI C'CATG_G Cuts at: 131 1313 131 Size: 1182 3224 NgoMIV G'CCGG_C Cuts at: 2585 2585 Size: 4406 NspI r_CATG'y Cuts at: 574 1101 1459 1959 2866 574 Size: 527 358 500 907 2114 Fragments arranged by size: 2114 907 527 500 358 PasI CC'CwG_GG Cuts at: 1164 1164 Size: 4406 PciI A'CATG_T Cuts at: 2862 2862 Size: 4406 PfoI T'CCnGG_A Cuts at: 1190 1190 Size: 4406 PmeI GTTT'AAAC Cuts at: 1405 1405 Size: 4406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 3545 Size: 32 4374 PpuMI rG'GwC_Cy Cuts at: 483 564 483 Size: 81 4325 PstI C_TGCA'G Cuts at: 924 1286 1453 2138 924 Size: 362 167 685 3192 Fragments arranged by size: 3192 685 362 167 PvuI CG_AT'CG Cuts at: 104 104 Size: 4406 PvuII CAG'CTG Cuts at: 180 441 1831 2191 180 Size: 261 1390 360 2395 Fragments arranged by size: 2395 1390 360 261 RsrII CG'GwC_CG Cuts at: 2601 2601 Size: 4406 SacI G_AGCT'C Cuts at: 1420 1420 Size: 4406 SacII CC_GC'GG Cuts at: 1259 1271 1259 Size: 12 4394 SalI G'TCGA_C Cuts at: 1443 1443 Size: 4406 SapI GCTCTTCn'nnn_ Cuts at: 2429 2639 2429 Size: 210 4196 SbfI CC_TGCA'GG Cuts at: 924 1453 924 Size: 529 3877 ScaI AGT'ACT Cuts at: 3923 3923 Size: 4406 SfcI C'TryA_G Cuts at: 33 188 327 468 920 1282 1449 2134 3127 Size: 155 139 141 452 362 167 685 993 Cuts at: 3127 3318 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 452 362 191 167 155 141 139 SfiI GGCCn_nnn'nGGCC Cuts at: 1309 1309 Size: 4406 SfoI GGC'GCC Cuts at: 1838 2086 1838 Size: 248 4158 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4406 SmaI CCC'GGG Cuts at: 584 1428 584 Size: 844 3562 SmlI C'TyrA_G Cuts at: 1306 1561 2476 2968 3230 1306 Size: 255 915 492 262 2482 Fragments arranged by size: 2482 915 492 262 255 SpeI A'CTAG_T Cuts at: 67 963 67 Size: 896 3510 SphI G_CATG'C Cuts at: 1459 1959 1459 Size: 500 3906 StyI C'CwwG_G Cuts at: 131 1313 1540 1894 131 Size: 1182 227 354 2643 Fragments arranged by size: 2643 1182 354 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 383 1169 1989 2122 2581 2790 383 Size: 786 820 133 459 209 1999 Fragments arranged by size: 1999 820 786 459 209 133 TatI w'GTAC_w Cuts at: 767 3921 767 Size: 3154 1252 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3923 3955 3923 Size: 32 4374 Tth111I GACn'n_nGTC Cuts at: 2203 2203 Size: 4406 XbaI T'CTAG_A Cuts at: 1437 1437 Size: 4406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1188 1901 1188 Size: 713 3693 XmaI C'CCGG_G Cuts at: 582 1426 582 Size: 844 3562 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI