Restriction map for
pF1KB7985
(Circular) (Six-base) MAPSORT of: pF1KB7985.seq Check: 8163 from: 1 to: 4406
pF1KB7985 4406 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 944 4246 944
Size: 3302 1104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2663 3410 3678 2231
Size: 432 747 268 2959
Fragments arranged by size:
2959 747 432 268
AflIII A'CryG_T
Cuts at: 824 1007 1758 2862 3839 824
Size: 183 751 1104 977 1391
Fragments arranged by size:
1391 1104 977 751 183
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AleI CACnn'nnGTG
Cuts at: 813 813
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1042 1711 3278 1042
Size: 669 1567 2170
Fragments arranged by size:
2170 1567 669
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 1021 1045 1410 3759 4015 1021
Size: 24 365 2349 256 1412
Fragments arranged by size:
2349 1412 365 256 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 582 811 1426 582
Size: 229 615 3562
Fragments arranged by size:
3562 615 229
BamHI G'GATC_C
Cuts at: 453 1431 453
Size: 978 3428
BanI G'GyrC_C
Cuts at: 148 1422 1836 2084 2119 148
Size: 1274 414 248 35 2435
Fragments arranged by size:
2435 1274 414 248 35
BanII G_rGCy'C
Cuts at: 410 1199 1420 2450 410
Size: 789 221 1030 2366
Fragments arranged by size:
2366 1030 789 221
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 584 711 584
Size: 127 4279
BbvCI CC'TCA_GC
Cuts at: 751 1330 751
Size: 579 3827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1009 1043 1009
Size: 34 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglI GCCn_nnn'nGGC
Cuts at: 1309 1309
Size: 4406
BglII A'GATC_T
Cuts at: 877 1921 3958 877
Size: 1044 2037 1325
Fragments arranged by size:
2037 1325 1044
BlpI GC'TnA_GC
Cuts at: 508 1518 508
Size: 1010 3396
Bme1580I G_kGCm'C
Cuts at: 187 2031 2124 3180 187
Size: 1844 93 1056 1413
Fragments arranged by size:
1844 1413 1056 93
BmgBI CAC'GTC
Cuts at: 315 315
Size: 4406
BmrI ACTGGGnnnn_n'
Cuts at: 285 1618 1699 2034 4178 285
Size: 1333 81 335 2144 513
Fragments arranged by size:
2144 1333 513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1213
Size: 4406
Bpu10I CC'TnA_GC
Cuts at: 513 751 1148 1330 513
Size: 238 397 182 3589
Fragments arranged by size:
3589 397 238 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 1582 2461 2953 3251 1291
Size: 291 879 492 298 2446
Fragments arranged by size:
2446 879 492 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 518 4110 518
Size: 3592 814
BsaAI yAC'GTr
Cuts at: 1008 1759 1008
Size: 751 3655
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 848 1595 2116 2794 3068 3215 3626 848
Size: 747 521 678 274 147 411 1628
Fragments arranged by size:
1628 747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 965 995 1219 1249 965
Size: 30 224 30 4122
Fragments arranged by size:
4122 224 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 739 1399 574
Size: 165 660 3581
Fragments arranged by size:
3581 660 165
BseYI C'CCAG_C
Cuts at: 176 180 350 834 1831 2589 3166 3662 176
Size: 4 170 484 997 758 577 496 920
Fragments arranged by size:
997 920 758 577 496 484 170 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 397 475 811 328
Size: 69 78 336 3923
Fragments arranged by size:
3923 336 78 69
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 848 1420 2198 2388 3180 848
Size: 572 778 190 792 2074
Fragments arranged by size:
2074 792 778 572 190
BsmBI CGTCTCn'nnnn_
Cuts at: 663 921 663
Size: 258 4148
Bsp1286I G_dGCh'C
Cuts at: 187 410 848 1199 1420 2031 2124 2198 2388
Size: 223 438 351 221 611 93 74 190
Cuts at: 2388 2450 3180 187
Size: 62 730 1413
Fragments arranged by size:
1413 730 611 438 351 223 221 190
93 74 62
BspEI T'CCGG_A
Cuts at: 848 3626 848
Size: 2778 1628
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1148 1456 1670 1972 2353 1148
Size: 308 214 302 381 3201
Fragments arranged by size:
3201 381 308 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 657 2318 657
Size: 1661 2745
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 207 478 2677 3035 207
Size: 271 2199 358 1578
Fragments arranged by size:
2199 1578 358 271
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 403 403
Size: 4406
BstYI r'GATC_y
Cuts at: 1 300 453 877 1431 1898 1921 2256 2502
Size: 299 153 424 554 467 23 335 246
Cuts at: 2502 3514 3600 3612 3958 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 554 467 449 424 346 335 299
246 153 86 23 12
BtgI C'CryG_G
Cuts at: 131 1239 1256 1268 1313 131
Size: 1108 17 12 45 3224
Fragments arranged by size:
3224 1108 45 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 1380 1649 1380
Size: 269 4137
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 381 1991 2165 2556 2583 3928 381
Size: 1610 174 391 27 1345 859
Fragments arranged by size:
1610 1345 859 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 694 2429 2639 694
Size: 1735 210 2461
Fragments arranged by size:
2461 1735 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 651 799 854 2614 2924 3070 4216 651
Size: 148 55 1760 310 146 1146 841
Fragments arranged by size:
1760 1146 841 310 148 146 55
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2231 2663 3410 3678 1213
Size: 1018 432 747 268 1941
Fragments arranged by size:
1941 1018 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 483 564 622 1302 1545 483
Size: 81 58 680 243 3344
Fragments arranged by size:
3344 680 243 81 58
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 215 1840 2088 3110 4089 215
Size: 1625 248 1022 979 532
Fragments arranged by size:
1625 1022 979 532 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 965 997 2248 2278 2280 2310 292
Size: 32 641 32 1251 30 2 30 2388
Fragments arranged by size:
2388 1251 641 32 32 30 30 2
HincII GTy'rAC
Cuts at: 318 1445 318
Size: 1127 3279
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 824 3839 824
Size: 3015 1391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 3077 3261 668
Size: 2409 184 1813
Fragments arranged by size:
2409 1813 184
MscI TGG'CCA
Cuts at: 2167 2167
Size: 4406
MslI CAynn'nnrTG
Cuts at: 813 2395 2522 813
Size: 1582 127 2697
Fragments arranged by size:
2697 1582 127
MspA1I CmG'CkG
Cuts at: 180 222 441 795 1258 1270 1517 1831 2191
Size: 42 219 354 463 12 247 314 360
Cuts at: 2191 3204 3449 180
Size: 1013 245 1137
Fragments arranged by size:
1137 1013 463 360 354 314 247 245
219 42 12
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 131 1313 131
Size: 1182 3224
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 574 1101 1459 1959 2866 574
Size: 527 358 500 907 2114
Fragments arranged by size:
2114 907 527 500 358
PasI CC'CwG_GG
Cuts at: 1164 1164
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PfoI T'CCnGG_A
Cuts at: 1190 1190
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PpuMI rG'GwC_Cy
Cuts at: 483 564 483
Size: 81 4325
PstI C_TGCA'G
Cuts at: 924 1286 1453 2138 924
Size: 362 167 685 3192
Fragments arranged by size:
3192 685 362 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 180 441 1831 2191 180
Size: 261 1390 360 2395
Fragments arranged by size:
2395 1390 360 261
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SacII CC_GC'GG
Cuts at: 1259 1271 1259
Size: 12 4394
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 924 1453 924
Size: 529 3877
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 188 327 468 920 1282 1449 2134 3127
Size: 155 139 141 452 362 167 685 993
Cuts at: 3127 3318 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 452 362 191 167 155
141 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 1309 1309
Size: 4406
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 584 1428 584
Size: 844 3562
SmlI C'TyrA_G
Cuts at: 1306 1561 2476 2968 3230 1306
Size: 255 915 492 262 2482
Fragments arranged by size:
2482 915 492 262 255
SpeI A'CTAG_T
Cuts at: 67 963 67
Size: 896 3510
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 131 1313 1540 1894 131
Size: 1182 227 354 2643
Fragments arranged by size:
2643 1182 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 383 1169 1989 2122 2581 2790 383
Size: 786 820 133 459 209 1999
Fragments arranged by size:
1999 820 786 459 209 133
TatI w'GTAC_w
Cuts at: 767 3921 767
Size: 3154 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1188 1901 1188
Size: 713 3693
XmaI C'CCGG_G
Cuts at: 582 1426 582
Size: 844 3562
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI