Restriction map for
pF1KB7987
(Circular) (Six-base) MAPSORT of: pF1KB7987.seq Check: 5017 from: 1 to: 4436
pF1KB7987 4436 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1474 1474
Size: 4436
Acc65I G'GTAC_C
Cuts at: 1250 1452 1250
Size: 202 4234
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 964 981 1039 1168 2261 2693 3440 3708
Size: 702 17 58 129 1093 432 747 268
Cuts at: 3708 262
Size: 990
Fragments arranged by size:
1093 990 747 702 432 268 129 58
17
AflIII A'CryG_T
Cuts at: 1788 2892 3869 1788
Size: 1104 977 2355
Fragments arranged by size:
2355 1104 977
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AhdI GACnn_n'nnGTC
Cuts at: 276 429 276
Size: 153 4283
AleI CACnn'nnGTG
Cuts at: 1418 1418
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 555 783 800 942 1297 1741 3308 555
Size: 228 17 142 355 444 1567 1683
Fragments arranged by size:
1683 1567 444 355 228 142 17
ApaI G_GGCC'C
Cuts at: 176 327 1015 176
Size: 151 688 3597
Fragments arranged by size:
3597 688 151
ApaLI G'TGCA_C
Cuts at: 979 1216 3206 979
Size: 237 1990 2209
Fragments arranged by size:
2209 1990 237
ApoI r'AATT_y
Cuts at: 1440 3789 4045 1440
Size: 2349 256 1831
Fragments arranged by size:
2349 1831 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 902 1396 1456 902
Size: 494 60 3882
Fragments arranged by size:
3882 494 60
BamHI G'GATC_C
Cuts at: 620 956 1133 1461 620
Size: 336 177 328 3595
Fragments arranged by size:
3595 336 328 177
BanI G'GyrC_C
Cuts at: 1250 1452 1866 2114 2149 1250
Size: 202 414 248 35 3537
Fragments arranged by size:
3537 414 248 202 35
BanII G_rGCy'C
Cuts at: 176 327 1015 1229 1450 2480 176
Size: 151 688 214 221 1030 2132
Fragments arranged by size:
2132 1030 688 221 214 151
BbeI G_GCGC'C
Cuts at: 1870 2118 1870
Size: 248 4188
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 259 259
Size: 4436
BglII A'GATC_T
Cuts at: 1951 3988 1951
Size: 2037 2399
BlpI GC'TnA_GC
Cuts at: 1548 1548
Size: 4436
Bme1580I G_kGCm'C
Cuts at: 176 327 470 568 983 1015 1220 2061 2154
Size: 151 143 98 415 32 205 841 93
Cuts at: 2154 3210 176
Size: 1056 1402
Fragments arranged by size:
1402 1056 841 415 205 151 143 98
93 32
BmgBI CAC'GTC
Cuts at: 249 249
Size: 4436
BmrI ACTGGGnnnn_n'
Cuts at: 1200 1648 1729 2064 4208 1200
Size: 448 81 335 2144 1428
Fragments arranged by size:
2144 1428 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 4436
Bpu10I CC'TnA_GC
Cuts at: 1223 1223
Size: 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1612 2491 2983 3281 1612
Size: 879 492 298 2767
Fragments arranged by size:
2767 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 625 1403 4140 625
Size: 778 2737 921
Fragments arranged by size:
2737 921 778
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 235 1950 3987 6
Size: 229 1715 2037 455
Fragments arranged by size:
2037 1715 455 229
BsaHI Gr'CG_yC
Cuts at: 149 475 604 1867 2115 2817 4313 149
Size: 326 129 1263 248 702 1496 272
Fragments arranged by size:
1496 1263 702 326 272 248 129
BsaWI w'CCGG_w
Cuts at: 395 1625 2146 2824 3098 3245 3656 395
Size: 1230 521 678 274 147 411 1175
Fragments arranged by size:
1230 1175 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1108 499
Size: 609 3827
BseYI C'CCAG_C
Cuts at: 750 1290 1315 1861 2619 3196 3692 750
Size: 540 25 546 758 577 496 1494
Fragments arranged by size:
1494 758 577 546 540 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 204 529 796 1026 163
Size: 41 325 267 230 3573
Fragments arranged by size:
3573 325 267 230 41
BsiEI CG_ry'CG
Cuts at: 104 437 494 1978 2024 3232 104
Size: 333 57 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 333 57 46
BsiHKAI G_wGCw'C
Cuts at: 710 833 983 1220 1229 1450 2228 2418 3210
Size: 123 150 237 9 221 778 190 792
Cuts at: 3210 710
Size: 1936
Fragments arranged by size:
1936 792 778 237 221 190 150 123
9
BsmBI CGTCTCn'nnnn_
Cuts at: 481 481
Size: 4436
Bsp1286I G_dGCh'C
Cuts at: 176 327 470 568 710 833 983 1015 1220
Size: 151 143 98 142 123 150 32 205
Cuts at: 1220 1229 1450 2061 2154 2228 2418 2480 3210
Size: 9 221 611 93 74 190 62 730
Cuts at: 3210 176
Size: 1402
Fragments arranged by size:
1402 730 611 221 205 190 151 150
143 142 123 98 93 74 62 32
9
BspEI T'CCGG_A
Cuts at: 395 3656 395
Size: 3261 1175
BspHI T'CATG_A
Cuts at: 164 3612 164
Size: 3448 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 1238 1486 1700 2002 2383 791
Size: 447 248 214 302 381 2844
Fragments arranged by size:
2844 447 381 302 248 214
BsrBI CCG'CTC
Cuts at: 50 744 2728 2782 4266 4431 50
Size: 694 1984 54 1484 165 55
Fragments arranged by size:
1984 1484 694 165 55 54
BsrDI GCAATG_nn'
Cuts at: 199 449 2348 199
Size: 250 1899 2287
Fragments arranged by size:
2287 1899 250
BsrFI r'CCGG_y
Cuts at: 527 2434 2615 2824 527
Size: 1907 181 209 2139
Fragments arranged by size:
2139 1907 209 181
BssSI C'ACGA_G
Cuts at: 569 982 2707 3065 569
Size: 413 1725 358 1940
Fragments arranged by size:
1940 1725 413 358
BstAPI GCAn_nnn'nTGC
Cuts at: 783 1813 783
Size: 1030 3406
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstYI r'GATC_y
Cuts at: 1 290 590 620 956 1109 1133 1461 1928
Size: 289 300 30 336 153 24 328 467
Cuts at: 1928 1951 2286 2532 3544 3630 3642 3988 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 336 335 328 300
289 246 153 86 30 24 23 12
BtgI C'CryG_G
Cuts at: 107 184 470 624 960 1335 107
Size: 77 286 154 336 375 3208
Fragments arranged by size:
3208 375 336 286 154 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 411 677 813 1679 411
Size: 266 136 866 3168
Fragments arranged by size:
3168 866 266 136
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DrdI GACnn_nn'nnGTC
Cuts at: 859 1210 2142 3000 859
Size: 351 932 858 2295
Fragments arranged by size:
2295 932 858 351
EaeI y'GGCC_r
Cuts at: 210 251 434 491 1380 2021 2195 2586 2613
Size: 41 183 57 889 641 174 391 27
Cuts at: 2613 3958 210
Size: 1345 688
Fragments arranged by size:
1345 889 688 641 391 183 174 57
41 27
EagI C'GGCC_G
Cuts at: 434 491 2021 434
Size: 57 1530 2849
Fragments arranged by size:
2849 1530 57
EarI CTCTTCn'nnn_
Cuts at: 715 793 2459 2669 715
Size: 78 1666 210 2482
Fragments arranged by size:
2482 1666 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1417 2644 2954 3100 4246 1417
Size: 1227 310 146 1146 1607
Fragments arranged by size:
1607 1227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1227 1448 1227
Size: 221 4215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 385 964 981 1039 1168 2261 2693 3440
Size: 123 579 17 58 129 1093 432 747
Cuts at: 3440 3708 262
Size: 268 990
Fragments arranged by size:
1093 990 747 579 432 268 129 123
58 17
EcoNI CCTnn'n_nnAGG
Cuts at: 310 946 1245 310
Size: 636 299 3501
Fragments arranged by size:
3501 636 299
EcoO109I rG'GnC_Cy
Cuts at: 135 173 303 323 324 360 780 881 939
Size: 38 130 20 1 36 420 101 58
Cuts at: 939 1011 1012 1575 135
Size: 72 1 563 2996
Fragments arranged by size:
2996 563 420 130 101 72 58 38
36 20 1 1
EcoRI G'AATT_C
Cuts at: 1440 1440
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4436
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 556 614 679 751 931 1322 1870 2118 3140
Size: 58 65 72 180 391 548 248 1022
Cuts at: 3140 4119 556
Size: 979 873
Fragments arranged by size:
1022 979 873 548 391 248 180 72
65 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 262 294 415 447 589 621 1060
Size: 32 84 32 121 32 142 32 439
Cuts at: 1060 1092 2278 2308 2310 2340 146
Size: 32 1186 30 2 30 2242
Fragments arranged by size:
2242 1186 439 142 121 84 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1161 1475 1161
Size: 314 4122
KasI G'GCGC_C
Cuts at: 1866 2114 1866
Size: 248 4188
KpnI G_GTAC'C
Cuts at: 1254 1456 1254
Size: 202 4234
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 177 710 968 3107 3291 177
Size: 533 258 2139 184 1322
Fragments arranged by size:
2139 1322 533 258 184
MscI TGG'CCA
Cuts at: 253 1382 2197 253
Size: 1129 815 2492
Fragments arranged by size:
2492 1129 815
MslI CAynn'nnrTG
Cuts at: 1084 1418 2425 2552 1084
Size: 334 1007 127 2968
Fragments arranged by size:
2968 1007 334 127
MspA1I CmG'CkG
Cuts at: 186 472 626 962 1294 1547 1861 2221 3234
Size: 286 154 336 332 253 314 360 1013
Cuts at: 3234 3479 186
Size: 245 1143
Fragments arranged by size:
1143 1013 360 336 332 314 286 253
245 154
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 1867 2115 1867
Size: 248 4188
NcoI C'CATG_G
Cuts at: 107 107
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NspI r_CATG'y
Cuts at: 1489 1989 2896 1489
Size: 500 907 3029
Fragments arranged by size:
3029 907 500
PciI A'CATG_T
Cuts at: 2892 2892
Size: 4436
PflMI CCAn_nnn'nTGG
Cuts at: 1190 1190
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4404
PpuMI rG'GwC_Cy
Cuts at: 303 360 780 303
Size: 57 420 3959
Fragments arranged by size:
3959 420 57
PspOMI G'GGCC_C
Cuts at: 172 323 1011 172
Size: 151 688 3597
Fragments arranged by size:
3597 688 151
PstI C_TGCA'G
Cuts at: 761 779 1483 2168 761
Size: 18 704 685 3029
Fragments arranged by size:
3029 704 685 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 1294 1861 2221 1294
Size: 567 360 3509
Fragments arranged by size:
3509 567 360
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1229 1450 1229
Size: 221 4215
SacII CC_GC'GG
Cuts at: 187 473 627 963 187
Size: 286 154 336 3660
Fragments arranged by size:
3660 336 286 154
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 715 793 2459 2669 715
Size: 78 1666 210 2482
Fragments arranged by size:
2482 1666 210 78
SbfI CC_TGCA'GG
Cuts at: 779 1483 779
Size: 704 3732
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SfcI C'TryA_G
Cuts at: 33 757 775 1242 1479 2164 3157 3348 33
Size: 724 18 467 237 685 993 191 1121
Fragments arranged by size:
1121 993 724 685 467 237 191 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 259 259
Size: 4436
SfoI GGC'GCC
Cuts at: 1868 2116 1868
Size: 248 4188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 904 1458 904
Size: 554 3882
SmlI C'TyrA_G
Cuts at: 1591 2506 2998 3260 1591
Size: 915 492 262 2767
Fragments arranged by size:
2767 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
StuI AGG'CCT
Cuts at: 691 691
Size: 4436
StyI C'CwwG_G
Cuts at: 107 635 686 1570 1924 107
Size: 528 51 884 354 2619
Fragments arranged by size:
2619 884 528 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1044 1201 2019 2152 2611 2820 1044
Size: 157 818 133 459 209 2660
Fragments arranged by size:
2660 818 459 209 157 133
TatI w'GTAC_w
Cuts at: 3951 3951
Size: 4436
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 390 422 1094 1126 3953 3985 390
Size: 32 672 32 2827 32 841
Fragments arranged by size:
2827 841 672 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 653 2233 653
Size: 1580 2856
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 1425 1931 360
Size: 1065 506 2865
Fragments arranged by size:
2865 1065 506
XmaI C'CCGG_G
Cuts at: 902 1456 902
Size: 554 3882
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI