Restriction map for
pF1KB7991
(Circular) (Six-base) MAPSORT of: pF1KB7991.seq Check: 1508 from: 1 to: 4439
pF1KB7991 4439 bp
With 182 enzymes: SgfI *
November 19, 2010 15:54 ..
AccI GT'mk_AC
Cuts at: 218 1477 218
Size: 1259 3180
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1336 2264 2696 3443 3711 1094
Size: 242 928 432 747 268 1822
Fragments arranged by size:
1822 928 747 432 268 242
AflIII A'CryG_T
Cuts at: 1791 2895 3872 1791
Size: 1104 977 2358
Fragments arranged by size:
2358 1104 977
AgeI A'CCGG_T
Cuts at: 2827 2827
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 1744 3311 1744
Size: 1567 2872
ApaI G_GGCC'C
Cuts at: 347 1289 347
Size: 942 3497
ApaLI G'TGCA_C
Cuts at: 3209 3209
Size: 4439
ApoI r'AATT_y
Cuts at: 1443 3792 4048 1443
Size: 2349 256 1834
Fragments arranged by size:
2349 1834 256
AscI GG'CGCG_CC
Cuts at: 558 558
Size: 4439
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 306 346 366 438 485 1459 306
Size: 40 20 72 47 974 3286
Fragments arranged by size:
3286 974 72 47 40 20
BamHI G'GATC_C
Cuts at: 1049 1464 1049
Size: 415 4024
BanI G'GyrC_C
Cuts at: 154 178 199 237 301 953 1455 1869 2117
Size: 24 21 38 64 652 502 414 248
Cuts at: 2117 2152 154
Size: 35 2441
Fragments arranged by size:
2441 652 502 414 248 64 38 35
24 21
BanII G_rGCy'C
Cuts at: 289 347 577 1289 1453 2483 289
Size: 58 230 712 164 1030 2245
Fragments arranged by size:
2245 1030 712 230 164 58
BbeI G_GCGC'C
Cuts at: 182 203 305 957 1873 2121 182
Size: 21 102 652 916 248 2500
Fragments arranged by size:
2500 916 652 248 102 21
BbvCI CC'TCA_GC
Cuts at: 878 878
Size: 4439
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3098 4439 2337
Size: 761 1341 2337
Fragments arranged by size:
2337 1341 761
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 163 539 686 700 908 163
Size: 376 147 14 208 3694
Fragments arranged by size:
3694 376 208 147 14
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 1551 1551
Size: 4439
Bme1580I G_kGCm'C
Cuts at: 227 242 347 1235 1289 2064 2157 3213 227
Size: 15 105 888 54 775 93 1056 1453
Fragments arranged by size:
1453 1056 888 775 105 93 54 15
BmgBI CAC'GTC
Cuts at: 612 612
Size: 4439
BmrI ACTGGGnnnn_n'
Cuts at: 1651 1732 2067 4211 1651
Size: 81 335 2144 1879
Fragments arranged by size:
2144 1879 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 1145 2806 928
Size: 217 1661 2561
Fragments arranged by size:
2561 1661 217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 567 810 1140 253
Size: 314 243 330 3552
Fragments arranged by size:
3552 330 314 243
Bpu10I CC'TnA_GC
Cuts at: 878 878
Size: 4439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2494 2986 3284 1615
Size: 879 492 298 2770
Fragments arranged by size:
2770 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4143 4143
Size: 4439
BsaAI yAC'GTr
Cuts at: 222 1792 222
Size: 1570 2869
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 179 200 302 954 1870 2118 2820 4316 179
Size: 21 102 652 916 248 702 1496 302
Fragments arranged by size:
1496 916 702 652 302 248 102 21
BsaWI w'CCGG_w
Cuts at: 295 319 1628 2149 2827 3101 3248 3659 295
Size: 24 1309 521 678 274 147 411 1075
Fragments arranged by size:
1309 1075 678 521 411 274 147 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1225 1255 1225
Size: 30 4409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 234 1198 234
Size: 964 3475
BseYI C'CCAG_C
Cuts at: 252 353 1864 2622 3199 3695 252
Size: 101 1511 758 577 496 996
Fragments arranged by size:
1511 996 758 577 496 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 4439
BsiEI CG_ry'CG
Cuts at: 104 464 690 704 865 1981 2027 3235 104
Size: 360 226 14 161 1116 46 1208 1308
Fragments arranged by size:
1308 1208 1116 360 226 161 46 14
BsiHKAI G_wGCw'C
Cuts at: 1453 2231 2421 3213 1453
Size: 778 190 792 2679
Fragments arranged by size:
2679 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1346 1346
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 227 242 289 347 577 1235 1289 1453 2064
Size: 15 47 58 230 658 54 164 611
Cuts at: 2064 2157 2231 2421 2483 3213 227
Size: 93 74 190 62 730 1453
Fragments arranged by size:
1453 730 658 611 230 190 164 93
74 62 58 54 47 15
BspEI T'CCGG_A
Cuts at: 295 3659 295
Size: 3364 1075
BspHI T'CATG_A
Cuts at: 3615 3615
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1489 1703 2005 2386 1489
Size: 214 302 381 3542
Fragments arranged by size:
3542 381 302 214
BsrBI CCG'CTC
Cuts at: 50 520 786 867 2731 2785 4269 4434 50
Size: 470 266 81 1864 54 1484 165 55
Fragments arranged by size:
1864 1484 470 266 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 966 990 1040 2351 966
Size: 24 50 1311 3054
Fragments arranged by size:
3054 1311 50 24
BsrFI r'CCGG_y
Cuts at: 414 562 649 860 884 2437 2618 2827 414
Size: 148 87 211 24 1553 181 209 2026
Fragments arranged by size:
2026 1553 211 209 181 148 87 24
BssHII G'CGCG_C
Cuts at: 558 558
Size: 4439
BssSI C'ACGA_G
Cuts at: 2710 3068 2710
Size: 358 4081
BstAPI GCAn_nnn'nTGC
Cuts at: 681 827 989 1816 681
Size: 146 162 827 3304
Fragments arranged by size:
3304 827 162 146
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstYI r'GATC_y
Cuts at: 1 1049 1464 1931 1954 2289 2535 3547 3633
Size: 1048 415 467 23 335 246 1012 86
Cuts at: 3633 3645 3991 1
Size: 12 346 449
Fragments arranged by size:
1048 1012 467 449 415 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 138 232 479 1011 1281 125
Size: 13 94 247 532 270 3283
Fragments arranged by size:
3283 532 270 247 94 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1285 2389 2570 2733 1285
Size: 1104 181 163 2991
Fragments arranged by size:
2991 1104 181 163
BtsI GCAGTG_nn'
Cuts at: 513 1122 1427 1682 513
Size: 609 305 255 3270
Fragments arranged by size:
3270 609 305 255
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DraIII CAC_nnn'GTG
Cuts at: 950 950
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 450 667 2145 3003 450
Size: 217 1478 858 1886
Fragments arranged by size:
1886 1478 858 217
EaeI y'GGCC_r
Cuts at: 122 128 461 605 687 701 862 1278 1403
Size: 6 333 144 82 14 161 416 125
Cuts at: 1403 2024 2198 2589 2616 3961 122
Size: 621 174 391 27 1345 600
Fragments arranged by size:
1345 621 600 416 391 333 174 161
144 125 82 27 14 6
EagI C'GGCC_G
Cuts at: 461 687 701 862 2024 461
Size: 226 14 161 1162 2876
Fragments arranged by size:
2876 1162 226 161 14
EarI CTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 290 530 636 882 2647 2957 3103 4249 290
Size: 240 106 246 1765 310 146 1146 480
Fragments arranged by size:
1765 1146 480 310 246 240 146 106
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 567 810 1094 1140 1336 2264 2696 3443
Size: 314 243 284 46 196 928 432 747
Cuts at: 3443 3711 253
Size: 268 981
Fragments arranged by size:
981 928 747 432 314 284 268 243
196 46
EcoO109I rG'GnC_Cy
Cuts at: 343 350 452 1017 1286 1578 343
Size: 7 102 565 269 292 3204
Fragments arranged by size:
3204 565 292 269 102 7
EcoRI G'AATT_C
Cuts at: 1443 1443
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4439
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 182 203 305 885 957 1873 2121 3143 4122
Size: 21 102 580 72 916 248 1022 979
Cuts at: 4122 182
Size: 499
Fragments arranged by size:
1022 979 916 580 499 248 102 72
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 724 743 756 775 2281 2311 2313 2343 724
Size: 19 13 19 1506 30 2 30 2820
Fragments arranged by size:
2820 1506 30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 762 1478 762
Size: 716 3723
KasI G'GCGC_C
Cuts at: 178 199 301 953 1869 2117 178
Size: 21 102 652 916 248 2500
Fragments arranged by size:
2500 916 652 248 102 21
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3110 3294 3110
Size: 184 4255
MscI TGG'CCA
Cuts at: 124 1280 2200 124
Size: 1156 920 2363
Fragments arranged by size:
2363 1156 920
MslI CAynn'nnrTG
Cuts at: 2428 2555 2428
Size: 127 4312
MspA1I CmG'CkG
Cuts at: 234 475 481 1139 1550 1864 2224 3237 3482
Size: 241 6 658 411 314 360 1013 245
Cuts at: 3482 234
Size: 1191
Fragments arranged by size:
1191 1013 658 411 360 314 245 241
6
NaeI GCC'GGC
Cuts at: 564 651 886 2620 564
Size: 87 235 1734 2383
Fragments arranged by size:
2383 1734 235 87
NarI GG'CG_CC
Cuts at: 179 200 302 954 1870 2118 179
Size: 21 102 652 916 248 2500
Fragments arranged by size:
2500 916 652 248 102 21
NcoI C'CATG_G
Cuts at: 125 1281 125
Size: 1156 3283
NgoMIV G'CCGG_C
Cuts at: 562 649 884 2618 562
Size: 87 235 1734 2383
Fragments arranged by size:
2383 1734 235 87
NotI GC'GGCC_GC
Cuts at: 461 461
Size: 4439
NspI r_CATG'y
Cuts at: 1492 1992 2899 1492
Size: 500 907 3032
Fragments arranged by size:
3032 907 500
PciI A'CATG_T
Cuts at: 2895 2895
Size: 4439
PflMI CCAn_nnn'nTGG
Cuts at: 950 950
Size: 4439
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 350 452 1017 350
Size: 102 565 3772
Fragments arranged by size:
3772 565 102
PspOMI G'GGCC_C
Cuts at: 343 1285 343
Size: 942 3497
PstI C_TGCA'G
Cuts at: 1486 2171 1486
Size: 685 3754
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 1864 2224 1864
Size: 360 4079
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SacII CC_GC'GG
Cuts at: 235 482 235
Size: 247 4192
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SanDI GG'GwC_CC
Cuts at: 350 452 1017 350
Size: 102 565 3772
Fragments arranged by size:
3772 565 102
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 501 1482 2167 3160 3351 33
Size: 468 981 685 993 191 1121
Fragments arranged by size:
1121 993 981 685 468 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 539 700 539
Size: 161 4278
SfoI GGC'GCC
Cuts at: 180 201 303 955 1871 2119 180
Size: 21 102 652 916 248 2500
Fragments arranged by size:
2500 916 652 248 102 21
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SgrAI Cr'CCGG_yG
Cuts at: 414 414
Size: 4439
SmaI CCC'GGG
Cuts at: 348 368 440 487 1461 348
Size: 20 72 47 974 3326
Fragments arranged by size:
3326 974 72 47 20
SmlI C'TyrA_G
Cuts at: 1594 2509 3001 3263 1594
Size: 915 492 262 2770
Fragments arranged by size:
2770 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
StuI AGG'CCT
Cuts at: 1026 1026
Size: 4439
StyI C'CwwG_G
Cuts at: 125 1281 1573 1927 125
Size: 1156 292 354 2637
Fragments arranged by size:
2637 1156 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2022 2155 2614 2823 2022
Size: 133 459 209 3638
Fragments arranged by size:
3638 459 209 133
TatI w'GTAC_w
Cuts at: 3954 3954
Size: 4439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1225 1257 3956 3988 1225
Size: 32 2699 32 1676
Fragments arranged by size:
2699 1676 32 32
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1934 453
Size: 1481 2958
XmaI C'CCGG_G
Cuts at: 346 366 438 485 1459 346
Size: 20 72 47 974 3326
Fragments arranged by size:
3326 974 72 47 20
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SexAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI