Restriction map for
pF1KB7995
(Circular) (Six-base) MAPSORT of: pF1KB7995.seq Check: 9860 from: 1 to: 4463
pF1KB7995 4463 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 2288 2720 3467 3735 325
Size: 1963 432 747 268 1053
Fragments arranged by size:
1963 1053 747 432 268
AflII C'TTAA_G
Cuts at: 997 997
Size: 4463
AflIII A'CryG_T
Cuts at: 335 1815 2919 3896 335
Size: 1480 1104 977 902
Fragments arranged by size:
1480 1104 977 902
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 341
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 1768 3335 1768
Size: 1567 2896
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 422 800 850 1042 1467 3816 4072 422
Size: 378 50 192 425 2349 256 813
Fragments arranged by size:
2349 813 425 378 256 192 50
AseI AT'TA_AT
Cuts at: 20 631 20
Size: 611 3852
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 620 1483 620
Size: 863 3600
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 969 1479 1893 2141 2176 969
Size: 510 414 248 35 3256
Fragments arranged by size:
3256 510 414 248 35
BanII G_rGCy'C
Cuts at: 876 1477 2507 876
Size: 601 1030 2832
Fragments arranged by size:
2832 1030 601
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BbsI GAAGACnn'nnnn_
Cuts at: 608 775 608
Size: 167 4296
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BfrBI ATG'CAT
Cuts at: 192 529 898 192
Size: 337 369 3757
Fragments arranged by size:
3757 369 337
BglII A'GATC_T
Cuts at: 512 1420 1978 4015 512
Size: 908 558 2037 960
Fragments arranged by size:
2037 960 908 558
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 2088 2181 3237 2088
Size: 93 1056 3314
Fragments arranged by size:
3314 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1675 1756 2091 4235 1099
Size: 576 81 335 2144 1327
Fragments arranged by size:
2144 1327 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1242 2830 1242
Size: 1588 2875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1212 827
Size: 385 4078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 2518 3010 3308 1639
Size: 879 492 298 2794
Fragments arranged by size:
2794 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4167 4167
Size: 4463
BsaAI yAC'GTr
Cuts at: 216 1816 216
Size: 1600 2863
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 494 1894 2142 2844 4340 494
Size: 1400 248 702 1496 617
Fragments arranged by size:
1496 1400 702 617 248
BsaWI w'CCGG_w
Cuts at: 476 1361 1652 2173 2851 3125 3272 3683 476
Size: 885 291 521 678 274 147 411 1256
Fragments arranged by size:
1256 885 678 521 411 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 588 618 588
Size: 30 4433
BseYI C'CCAG_C
Cuts at: 1888 2646 3223 3719 1888
Size: 758 577 496 2632
Fragments arranged by size:
2632 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 374 203
Size: 171 4292
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3259 104
Size: 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 1477 2255 2445 3237 176
Size: 1301 778 190 792 1402
Fragments arranged by size:
1402 1301 792 778 190
BsmI GAATG_Cn'
Cuts at: 194 557 194
Size: 363 4100
Bsp1286I G_dGCh'C
Cuts at: 176 876 1477 2088 2181 2255 2445 2507 3237
Size: 700 601 611 93 74 190 62 730
Cuts at: 3237 176
Size: 1402
Fragments arranged by size:
1402 730 700 611 601 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 497 3639 497
Size: 3142 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1513 1727 2029 2410 1513
Size: 214 302 381 3566
Fragments arranged by size:
3566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2755 2809 4293 4458 50
Size: 2705 54 1484 165 55
Fragments arranged by size:
2705 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 2461 2642 2851 2461
Size: 181 209 4073
Fragments arranged by size:
4073 209 181
BsrGI T'GTAC_A
Cuts at: 338 338
Size: 4463
BssSI C'ACGA_G
Cuts at: 2734 3092 2734
Size: 358 4105
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstYI r'GATC_y
Cuts at: 1 512 1420 1488 1955 1978 2313 2559 3571
Size: 511 908 68 467 23 335 246 1012
Cuts at: 3571 3657 3669 4015 1
Size: 86 12 346 449
Fragments arranged by size:
1012 908 511 467 449 346 335 246
86 68 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 1706 1706
Size: 4463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1201 1236 1201
Size: 35 4428
DraI TTT'AAA
Cuts at: 509 1373 1462 509
Size: 864 89 3510
Fragments arranged by size:
3510 864 89
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 227 1243 2048 2222 2613 2640 3985 227
Size: 1016 805 174 391 27 1345 705
Fragments arranged by size:
1345 1016 805 705 391 174 27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 823 1211 1411 2486 2696 823
Size: 388 200 1075 210 2590
Fragments arranged by size:
2590 1075 388 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 2981 3127 4273 2671
Size: 310 146 1146 2861
Fragments arranged by size:
2861 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 827 1212 2288 2720 3467 3735 325
Size: 502 385 1076 432 747 268 1053
Fragments arranged by size:
1076 1053 747 502 432 385 268
EcoNI CCTnn'n_nnAGG
Cuts at: 643 643
Size: 4463
EcoO109I rG'GnC_Cy
Cuts at: 136 816 1602 136
Size: 680 786 2997
Fragments arranged by size:
2997 786 680
EcoRI G'AATT_C
Cuts at: 850 1042 1467 850
Size: 192 425 3846
Fragments arranged by size:
3846 425 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 534 2482 534
Size: 1948 2515
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1897 2145 3167 4146 1897
Size: 248 1022 979 2214
Fragments arranged by size:
2214 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 993 1025 2305 2335 2337 2367 993
Size: 32 1280 30 2 30 3089
Fragments arranged by size:
3089 1280 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
HindIII A'AGCT_T
Cuts at: 706 1316 706
Size: 610 3853
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1076 3134 3318 1019
Size: 57 2058 184 2164
Fragments arranged by size:
2164 2058 184 57
MscI TGG'CCA
Cuts at: 229 1245 2224 229
Size: 1016 979 2468
Fragments arranged by size:
2468 1016 979
MslI CAynn'nnrTG
Cuts at: 2452 2579 2452
Size: 127 4336
MspA1I CmG'CkG
Cuts at: 764 1574 1888 2248 3261 3506 764
Size: 810 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 810 360 314 245
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NdeI CA'TA_TG
Cuts at: 900 900
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NsiI A_TGCA'T
Cuts at: 194 531 900 194
Size: 337 369 3757
Fragments arranged by size:
3757 369 337
NspI r_CATG'y
Cuts at: 339 529 1516 2016 2923 339
Size: 190 987 500 907 1879
Fragments arranged by size:
1879 987 907 500 190
PciI A'CATG_T
Cuts at: 335 2919 335
Size: 2584 1879
PfoI T'CCnGG_A
Cuts at: 804 1228 804
Size: 424 4039
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 816 816
Size: 4463
PstI C_TGCA'G
Cuts at: 330 1510 2195 330
Size: 1180 685 2598
Fragments arranged by size:
2598 1180 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 764 1888 2248 764
Size: 1124 360 2979
Fragments arranged by size:
2979 1124 360
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SanDI GG'GwC_CC
Cuts at: 816 816
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SexAI A'CCwGG_T
Cuts at: 676 676
Size: 4463
SfcI C'TryA_G
Cuts at: 33 326 1506 2191 3184 3375 33
Size: 293 1180 685 993 191 1121
Fragments arranged by size:
1180 1121 993 685 293 191
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 622 1485 622
Size: 863 3600
SmlI C'TyrA_G
Cuts at: 997 1618 2533 3025 3287 997
Size: 621 915 492 262 2173
Fragments arranged by size:
2173 915 621 492 262
SnaBI TAC'GTA
Cuts at: 216 216
Size: 4463
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
SspI AAT'ATT
Cuts at: 489 629 783 489
Size: 140 154 4169
Fragments arranged by size:
4169 154 140
StuI AGG'CCT
Cuts at: 649 649
Size: 4463
StyI C'CwwG_G
Cuts at: 199 356 1597 1951 199
Size: 157 1241 354 2711
Fragments arranged by size:
2711 1241 354 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2046 2179 2638 2847 2046
Size: 133 459 209 3662
Fragments arranged by size:
3662 459 209 133
TatI w'GTAC_w
Cuts at: 338 3978 338
Size: 3640 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 588 620 3980 4012 588
Size: 32 3360 32 1039
Fragments arranged by size:
3360 1039 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1958 447
Size: 1511 2952
XmaI C'CCGG_G
Cuts at: 620 1483 620
Size: 863 3600
XmnI GAAnn'nnTTC
Cuts at: 911 911
Size: 4463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NheI NotI NruI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI
SrfI SwaI XhoI ZraI