Restriction map for
pF1KB8003
(Circular) (Six-base) MAPSORT of: pF1KB8003.seq Check: 1422 from: 1 to: 3602
pF1KB8003 3602 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 293 293
Size: 3602
AccI GT'mk_AC
Cuts at: 640 640
Size: 3602
Acc65I G'GTAC_C
Cuts at: 618 618
Size: 3602
AclI AA'CG_TT
Cuts at: 3442 3442
Size: 3602
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 1427 1859 2606 2874 465
Size: 962 432 747 268 1193
Fragments arranged by size:
1193 962 747 432 268
AflIII A'CryG_T
Cuts at: 954 2058 3035 954
Size: 1104 977 1521
Fragments arranged by size:
1521 1104 977
AgeI A'CCGG_T
Cuts at: 1990 1990
Size: 3602
AhdI GACnn_n'nnGTC
Cuts at: 417 417
Size: 3602
AlwNI CAG_nnn'CTG
Cuts at: 443 907 2474 443
Size: 464 1567 1571
Fragments arranged by size:
1571 1567 464
ApaI G_GGCC'C
Cuts at: 554 554
Size: 3602
ApaLI G'TGCA_C
Cuts at: 2372 2372
Size: 3602
ApoI r'AATT_y
Cuts at: 606 2955 3211 606
Size: 2349 256 997
Fragments arranged by size:
2349 997 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3602
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
AvaI C'yCGr_G
Cuts at: 622 622
Size: 3602
BamHI G'GATC_C
Cuts at: 627 627
Size: 3602
BanI G'GyrC_C
Cuts at: 475 618 1032 1280 1315 475
Size: 143 414 248 35 2762
Fragments arranged by size:
2762 414 248 143 35
BanII G_rGCy'C
Cuts at: 554 616 1646 554
Size: 62 1030 2510
Fragments arranged by size:
2510 1030 62
BbeI G_GCGC'C
Cuts at: 1036 1284 1036
Size: 248 3354
BbsI GAAGACnn'nnnn_
Cuts at: 518 518
Size: 3602
BciVI GTATCCnnnnn_n'
Cuts at: 1500 2261 3602 1500
Size: 761 1341 1500
Fragments arranged by size:
1500 1341 761
BclI T'GATC_A
Cuts at: 371 1122 371
Size: 751 2851
BglII A'GATC_T
Cuts at: 1117 3154 1117
Size: 2037 1565
BlpI GC'TnA_GC
Cuts at: 450 714 450
Size: 264 3338
Bme1580I G_kGCm'C
Cuts at: 554 1227 1320 2376 554
Size: 673 93 1056 1780
Fragments arranged by size:
1780 1056 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 814 895 1230 3374 814
Size: 81 335 2144 1042
Fragments arranged by size:
2144 1042 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 3602
Bpu10I CC'TnA_GC
Cuts at: 162 162
Size: 3602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1657 2149 2447 778
Size: 879 492 298 1933
Fragments arranged by size:
1933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 461 3306 461
Size: 2845 757
BsaAI yAC'GTr
Cuts at: 955 955
Size: 3602
BsaBI GATnn'nnATC
Cuts at: 6 1116 3153 6
Size: 1110 2037 455
Fragments arranged by size:
2037 1110 455
BsaHI Gr'CG_yC
Cuts at: 515 1033 1281 1983 3479 515
Size: 518 248 702 1496 638
Fragments arranged by size:
1496 702 638 518 248
BsaWI w'CCGG_w
Cuts at: 791 1312 1990 2264 2411 2822 791
Size: 521 678 274 147 411 1571
Fragments arranged by size:
1571 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 370 421 133
Size: 237 51 3314
Fragments arranged by size:
3314 237 51
BseYI C'CCAG_C
Cuts at: 137 217 1027 1785 2362 2858 137
Size: 80 810 758 577 496 881
Fragments arranged by size:
881 810 758 577 496 80
BsiEI CG_ry'CG
Cuts at: 104 1144 1190 2398 104
Size: 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 46
BsiHKAI G_wGCw'C
Cuts at: 616 1394 1584 2376 616
Size: 778 190 792 1842
Fragments arranged by size:
1842 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 554 616 1227 1320 1394 1584 1646 2376 554
Size: 62 611 93 74 190 62 730 1780
Fragments arranged by size:
1780 730 611 190 93 74 62 62
BspEI T'CCGG_A
Cuts at: 2822 2822
Size: 3602
BspHI T'CATG_A
Cuts at: 2778 2778
Size: 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 652 866 1168 1549 293
Size: 359 214 302 381 2346
Fragments arranged by size:
2346 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 213 548 1894 1948 3432 3597 50
Size: 163 335 1346 54 1484 165 55
Fragments arranged by size:
1484 1346 335 165 163 55 54
BsrDI GCAATG_nn'
Cuts at: 1514 1514
Size: 3602
BsrFI r'CCGG_y
Cuts at: 1600 1781 1990 1600
Size: 181 209 3212
Fragments arranged by size:
3212 209 181
BssSI C'ACGA_G
Cuts at: 1873 2231 1873
Size: 358 3244
BstAPI GCAn_nnn'nTGC
Cuts at: 979 979
Size: 3602
BstBI TT'CG_AA
Cuts at: 1963 1963
Size: 3602
BstYI r'GATC_y
Cuts at: 1 627 1094 1117 1452 1698 2710 2796 2808
Size: 626 467 23 335 246 1012 86 12
Cuts at: 2808 3154 1
Size: 346 449
Fragments arranged by size:
1012 626 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1552 1733 1896 1552
Size: 181 163 3258
Fragments arranged by size:
3258 181 163
BtsI GCAGTG_nn'
Cuts at: 240 845 240
Size: 605 2997
DraI TTT'AAA
Cuts at: 601 601
Size: 3602
DrdI GACnn_nn'nnGTC
Cuts at: 1308 2166 1308
Size: 858 2744
EaeI y'GGCC_r
Cuts at: 1187 1361 1752 1779 3124 1187
Size: 174 391 27 1345 1665
Fragments arranged by size:
1665 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1187 1187
Size: 3602
EarI CTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1810 2120 2266 3412 127
Size: 1683 310 146 1146 317
Fragments arranged by size:
1683 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 614 614
Size: 3602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 465 1427 1859 2606 2874 385
Size: 80 962 432 747 268 1113
Fragments arranged by size:
1113 962 747 432 268 80
EcoO109I rG'GnC_Cy
Cuts at: 360 741 360
Size: 381 3221
EcoRI G'AATT_C
Cuts at: 606 606
Size: 3602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 1621
Size: 3602
FspI TGC'GCA
Cuts at: 1383 1383
Size: 3602
HaeII r_GCGC'y
Cuts at: 1036 1284 2306 3285 1036
Size: 248 1022 979 1353
Fragments arranged by size:
1353 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 433 435 465 1444 1474 1476 1506 403
Size: 30 2 30 979 30 2 30 2499
Fragments arranged by size:
2499 979 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 641 641
Size: 3602
KasI G'GCGC_C
Cuts at: 1032 1280 1032
Size: 248 3354
KpnI G_GTAC'C
Cuts at: 622 622
Size: 3602
MluI A'CGCG_T
Cuts at: 3035 3035
Size: 3602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2457 2273
Size: 184 3418
MscI TGG'CCA
Cuts at: 1363 1363
Size: 3602
MslI CAynn'nnrTG
Cuts at: 1591 1718 1591
Size: 127 3475
MspA1I CmG'CkG
Cuts at: 221 275 713 1027 1387 2400 2645 221
Size: 54 438 314 360 1013 245 1178
Fragments arranged by size:
1178 1013 438 360 314 245 54
NaeI GCC'GGC
Cuts at: 1783 1783
Size: 3602
NarI GG'CG_CC
Cuts at: 1033 1281 1033
Size: 248 3354
NcoI C'CATG_G
Cuts at: 107 107
Size: 3602
NgoMIV G'CCGG_C
Cuts at: 1781 1781
Size: 3602
NspI r_CATG'y
Cuts at: 655 1155 2062 655
Size: 500 907 2195
Fragments arranged by size:
2195 907 500
PciI A'CATG_T
Cuts at: 2058 2058
Size: 3602
PflMI CCAn_nnn'nTGG
Cuts at: 206 206
Size: 3602
PmeI GTTT'AAAC
Cuts at: 601 601
Size: 3602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 3570
PspOMI G'GGCC_C
Cuts at: 550 550
Size: 3602
PstI C_TGCA'G
Cuts at: 448 649 1334 448
Size: 201 685 2716
Fragments arranged by size:
2716 685 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3602
PvuII CAG'CTG
Cuts at: 1027 1387 1027
Size: 360 3242
RsrII CG'GwC_CG
Cuts at: 1797 1797
Size: 3602
SacI G_AGCT'C
Cuts at: 616 616
Size: 3602
SalI G'TCGA_C
Cuts at: 639 639
Size: 3602
SapI GCTCTTCn'nnn_
Cuts at: 1625 1835 1625
Size: 210 3392
SbfI CC_TGCA'GG
Cuts at: 649 649
Size: 3602
ScaI AGT'ACT
Cuts at: 3119 3119
Size: 3602
SfcI C'TryA_G
Cuts at: 33 444 645 1330 2323 2514 33
Size: 411 201 685 993 191 1121
Fragments arranged by size:
1121 993 685 411 201 191
SfoI GGC'GCC
Cuts at: 1034 1282 1034
Size: 248 3354
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3602
SmaI CCC'GGG
Cuts at: 624 624
Size: 3602
SmlI C'TyrA_G
Cuts at: 757 1672 2164 2426 757
Size: 915 492 262 1933
Fragments arranged by size:
1933 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3602
SphI G_CATG'C
Cuts at: 655 1155 655
Size: 500 3102
StyI C'CwwG_G
Cuts at: 107 350 455 736 1090 107
Size: 243 105 281 354 2619
Fragments arranged by size:
2619 354 281 243 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 1185 1318 1777 1986 463
Size: 722 133 459 209 2079
Fragments arranged by size:
2079 722 459 209 133
TatI w'GTAC_w
Cuts at: 173 3117 173
Size: 2944 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3570
Tth111I GACn'n_nGTC
Cuts at: 1399 1399
Size: 3602
XbaI T'CTAG_A
Cuts at: 633 633
Size: 3602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 1097 468
Size: 629 2973
XmaI C'CCGG_G
Cuts at: 622 622
Size: 3602
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI