Restriction map for
pF1KB8004
(Circular) (Six-base) MAPSORT of: pF1KB8004.seq Check: 3274 from: 1 to: 5225
pF1KB8004 5225 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 629 836 2263 629
Size: 207 1427 3591
Fragments arranged by size:
3591 1427 207
Acc65I G'GTAC_C
Cuts at: 169 1529 2241 169
Size: 1360 712 3153
Fragments arranged by size:
3153 1360 712
AclI AA'CG_TT
Cuts at: 5065 5065
Size: 5225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 608 665 757 1422 3050 3482 4229 4497
Size: 22 57 92 665 1628 432 747 268
Cuts at: 4497 586
Size: 1314
Fragments arranged by size:
1628 1314 747 665 432 268 92 57
22
AfeI AGC'GCT
Cuts at: 1892 1892
Size: 5225
AflIII A'CryG_T
Cuts at: 2577 3681 4658 2577
Size: 1104 977 3144
Fragments arranged by size:
3144 1104 977
AgeI A'CCGG_T
Cuts at: 3613 3613
Size: 5225
AleI CACnn'nnGTG
Cuts at: 2026 2026
Size: 5225
AlwNI CAG_nnn'CTG
Cuts at: 318 869 2094 2530 4097 318
Size: 551 1225 436 1567 1446
Fragments arranged by size:
1567 1446 1225 551 436
ApaI G_GGCC'C
Cuts at: 1724 1724
Size: 5225
ApaLI G'TGCA_C
Cuts at: 3995 3995
Size: 5225
ApoI r'AATT_y
Cuts at: 2229 4578 4834 2229
Size: 2349 256 2620
Fragments arranged by size:
2620 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
AvaI C'yCGr_G
Cuts at: 134 1505 2178 2245 134
Size: 1371 673 67 3114
Fragments arranged by size:
3114 1371 673 67
BamHI G'GATC_C
Cuts at: 1229 2250 1229
Size: 1021 4204
BanI G'GyrC_C
Cuts at: 169 528 1529 2067 2241 2655 2903 2938 169
Size: 359 1001 538 174 414 248 35 2456
Fragments arranged by size:
2456 1001 538 414 359 248 174 35
BanII G_rGCy'C
Cuts at: 1304 1724 1787 1831 1841 2239 3269 1304
Size: 420 63 44 10 398 1030 3260
Fragments arranged by size:
3260 1030 420 398 63 44 10
BbeI G_GCGC'C
Cuts at: 2071 2659 2907 2071
Size: 588 248 4389
Fragments arranged by size:
4389 588 248
BbsI GAAGACnn'nnnn_
Cuts at: 1064 1510 1064
Size: 446 4779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 369 403 736 770 369
Size: 34 333 34 4824
Fragments arranged by size:
4824 333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 556 3123 3884 5225 556
Size: 2567 761 1341 556
Fragments arranged by size:
2567 1341 761 556
BclI T'GATC_A
Cuts at: 2745 2745
Size: 5225
BglI GCCn_nnn'nGGC
Cuts at: 2065 2082 2065
Size: 17 5208
BglII A'GATC_T
Cuts at: 1196 2740 4777 1196
Size: 1544 2037 1644
Fragments arranged by size:
2037 1644 1544
BlpI GC'TnA_GC
Cuts at: 2337 2337
Size: 5225
Bme1580I G_kGCm'C
Cuts at: 533 1724 2850 2943 3999 533
Size: 1191 1126 93 1056 1759
Fragments arranged by size:
1759 1191 1126 1056 93
BmgBI CAC'GTC
Cuts at: 1152 1152
Size: 5225
BmrI ACTGGGnnnn_n'
Cuts at: 499 536 642 1318 2437 2518 2853 4997 499
Size: 37 106 676 1119 81 335 2144 727
Fragments arranged by size:
2144 1119 727 676 335 106 81 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 5225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 841 1141 1234 285
Size: 556 300 93 4276
Fragments arranged by size:
4276 556 300 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 1240 1843 2401 3280 3772 4070 384
Size: 856 603 558 879 492 298 1539
Fragments arranged by size:
1539 879 856 603 558 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1806 4929 1806
Size: 3123 2102
BsaAI yAC'GTr
Cuts at: 2578 2578
Size: 5225
BsaBI GATnn'nnATC
Cuts at: 6 499 2739 4776 6
Size: 493 2240 2037 455
Fragments arranged by size:
2240 2037 493 455
BsaHI Gr'CG_yC
Cuts at: 1625 2068 2074 2656 2904 3606 5102 1625
Size: 443 6 582 248 702 1496 1748
Fragments arranged by size:
1748 1496 702 582 443 248 6
BsaWI w'CCGG_w
Cuts at: 1397 1833 2414 2935 3613 3887 4034 4445 1397
Size: 436 581 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 581 521 436 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 1078 1108 1125 1155 1192 1222 1400
Size: 30 918 30 17 30 37 30 178
Cuts at: 1400 1430 1696 1726 1828 1858 130
Size: 30 266 30 102 30 3497
Fragments arranged by size:
3497 918 266 178 102 37 30 30
30 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 1291 1795 2145 247
Size: 1044 504 350 3327
Fragments arranged by size:
3327 1044 504 350
BseYI C'CCAG_C
Cuts at: 251 336 532 862 1357 1973 2650 3408 3985
Size: 85 196 330 495 616 677 758 577
Cuts at: 3985 4481 251
Size: 496 995
Fragments arranged by size:
995 758 677 616 577 496 495 330
196 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1328
Size: 5225
BsiEI CG_ry'CG
Cuts at: 104 1160 2767 2813 4021 104
Size: 1056 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 1056 46
BsiHKAI G_wGCw'C
Cuts at: 1787 1831 2033 2239 3017 3207 3999 1787
Size: 44 202 206 778 190 792 3013
Fragments arranged by size:
3013 792 778 206 202 190 44
BsmI GAATG_Cn'
Cuts at: 381 381
Size: 5225
BsmBI CGTCTCn'nnnn_
Cuts at: 1158 1158
Size: 5225
Bsp1286I G_dGCh'C
Cuts at: 533 1304 1724 1787 1831 1841 2033 2239 2850
Size: 771 420 63 44 10 192 206 611
Cuts at: 2850 2943 3017 3207 3269 3999 533
Size: 93 74 190 62 730 1759
Fragments arranged by size:
1759 771 730 611 420 206 192 190
93 74 63 62 44 10
BspEI T'CCGG_A
Cuts at: 4445 4445
Size: 5225
BspHI T'CATG_A
Cuts at: 908 4401 908
Size: 3493 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2275 2489 2791 3172 2275
Size: 214 302 381 4328
Fragments arranged by size:
4328 381 302 214
BsrBI CCG'CTC
Cuts at: 50 722 3517 3571 5055 5220 50
Size: 672 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 672 165 55 54
BsrDI GCAATG_nn'
Cuts at: 480 3137 480
Size: 2657 2568
BsrFI r'CCGG_y
Cuts at: 2108 3223 3404 3613 2108
Size: 1115 181 209 3720
Fragments arranged by size:
3720 1115 209 181
BssSI C'ACGA_G
Cuts at: 3496 3854 3496
Size: 358 4867
BstAPI GCAn_nnn'nTGC
Cuts at: 2602 2602
Size: 5225
BstBI TT'CG_AA
Cuts at: 3586 3586
Size: 5225
BstXI CCAn_nnnn'nTGG
Cuts at: 2134 2134
Size: 5225
BstYI r'GATC_y
Cuts at: 1 844 1196 1229 2250 2717 2740 3075 3321
Size: 843 352 33 1021 467 23 335 246
Cuts at: 3321 4333 4419 4431 4777 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1021 1012 843 467 449 352 346 335
246 86 33 23 12
Bsu36I CC'TnA_GG
Cuts at: 1292 1292
Size: 5225
BtgI C'CryG_G
Cuts at: 107 793 1175 1716 1796 107
Size: 686 382 541 80 3536
Fragments arranged by size:
3536 686 541 382 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3175 3356 3519 3175
Size: 181 163 4881
Fragments arranged by size:
4881 181 163
BtsI GCAGTG_nn'
Cuts at: 385 1971 2468 385
Size: 1586 497 3142
Fragments arranged by size:
3142 1586 497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 389 424 389
Size: 35 5190
DraI TTT'AAA
Cuts at: 975 2224 975
Size: 1249 3976
DraIII CAC_nnn'GTG
Cuts at: 351 1747 351
Size: 1396 3829
DrdI GACnn_nn'nnGTC
Cuts at: 2931 3789 2931
Size: 858 4367
EaeI y'GGCC_r
Cuts at: 110 1157 2810 2984 3375 3402 4747 110
Size: 1047 1653 174 391 27 1345 588
Fragments arranged by size:
1653 1345 1047 588 391 174 27
EagI C'GGCC_G
Cuts at: 1157 2810 1157
Size: 1653 3572
EarI CTCTTCn'nnn_
Cuts at: 452 2038 3248 3458 452
Size: 1586 1210 210 2219
Fragments arranged by size:
2219 1586 1210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 1657 3433 3743 3889 5035 199
Size: 1458 1776 310 146 1146 389
Fragments arranged by size:
1776 1458 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 1785 1829 2237 1785
Size: 44 408 4773
Fragments arranged by size:
4773 408 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 586 608 665 757 841 1141 1234 1422
Size: 301 22 57 92 84 300 93 188
Cuts at: 1422 3050 3482 4229 4497 285
Size: 1628 432 747 268 1013
Fragments arranged by size:
1628 1013 747 432 301 300 268 188
93 92 84 57 22
EcoNI CCTnn'n_nnAGG
Cuts at: 217 229 1491 1558 217
Size: 12 1262 67 3884
Fragments arranged by size:
3884 1262 67 12
EcoO109I rG'GnC_Cy
Cuts at: 222 636 676 1287 1605 1721 2194 2364 222
Size: 414 40 611 318 116 473 170 3083
Fragments arranged by size:
3083 611 473 414 318 170 116 40
EcoRI G'AATT_C
Cuts at: 2229 2229
Size: 5225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 5225
FspI TGC'GCA
Cuts at: 3006 3006
Size: 5225
HaeII r_GCGC'y
Cuts at: 966 1894 2071 2659 2907 3929 4908 966
Size: 928 177 588 248 1022 979 1283
Fragments arranged by size:
1283 1022 979 928 588 248 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 264 296 829 861 1400 1432 1828
Size: 32 102 32 533 32 539 32 396
Cuts at: 1828 1860 2161 2193 3067 3097 3099 3129 130
Size: 32 301 32 874 30 2 30 2226
Fragments arranged by size:
2226 874 539 533 396 301 102 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2264 2264
Size: 5225
HindIII A'AGCT_T
Cuts at: 402 739 402
Size: 337 4888
KasI G'GCGC_C
Cuts at: 2067 2655 2903 2067
Size: 588 248 4389
Fragments arranged by size:
4389 588 248
KpnI G_GTAC'C
Cuts at: 173 1533 2245 173
Size: 1360 712 3153
Fragments arranged by size:
3153 1360 712
MluI A'CGCG_T
Cuts at: 4658 4658
Size: 5225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1548 3896 4080 1548
Size: 2348 184 2693
Fragments arranged by size:
2693 2348 184
MscI TGG'CCA
Cuts at: 112 2986 112
Size: 2874 2351
MslI CAynn'nnrTG
Cuts at: 1900 2026 3214 3341 1900
Size: 126 1188 127 3784
Fragments arranged by size:
3784 1188 127 126
MspA1I CmG'CkG
Cuts at: 812 1469 1718 2336 2650 3010 4023 4268 812
Size: 657 249 618 314 360 1013 245 1769
Fragments arranged by size:
1769 1013 657 618 360 314 249 245
NaeI GCC'GGC
Cuts at: 3406 3406
Size: 5225
NarI GG'CG_CC
Cuts at: 2068 2656 2904 2068
Size: 588 248 4389
Fragments arranged by size:
4389 588 248
NcoI C'CATG_G
Cuts at: 107 1796 107
Size: 1689 3536
NgoMIV G'CCGG_C
Cuts at: 3404 3404
Size: 5225
NspI r_CATG'y
Cuts at: 247 1597 2278 2778 3685 247
Size: 1350 681 500 907 1787
Fragments arranged by size:
1787 1350 907 681 500
PciI A'CATG_T
Cuts at: 3681 3681
Size: 5225
PflMI CCAn_nnn'nTGG
Cuts at: 1747 1747
Size: 5225
PfoI T'CCnGG_A
Cuts at: 947 1009 947
Size: 62 5163
PmeI GTTT'AAAC
Cuts at: 2224 2224
Size: 5225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 4364
Size: 32 5193
PpuMI rG'GwC_Cy
Cuts at: 222 636 2194 222
Size: 414 1558 3253
Fragments arranged by size:
3253 1558 414
PspOMI G'GGCC_C
Cuts at: 1720 1720
Size: 5225
PstI C_TGCA'G
Cuts at: 1192 1577 2090 2272 2957 1192
Size: 385 513 182 685 3460
Fragments arranged by size:
3460 685 513 385 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5225
PvuII CAG'CTG
Cuts at: 1469 2650 3010 1469
Size: 1181 360 3684
Fragments arranged by size:
3684 1181 360
RsrII CG'GwC_CG
Cuts at: 3420 3420
Size: 5225
SacI G_AGCT'C
Cuts at: 1787 1831 2239 1787
Size: 44 408 4773
Fragments arranged by size:
4773 408 44
SacII CC_GC'GG
Cuts at: 1719 1719
Size: 5225
SalI G'TCGA_C
Cuts at: 2262 2262
Size: 5225
SapI GCTCTTCn'nnn_
Cuts at: 452 2038 3248 3458 452
Size: 1586 1210 210 2219
Fragments arranged by size:
2219 1586 1210 210
SbfI CC_TGCA'GG
Cuts at: 1577 2272 1577
Size: 695 4530
ScaI AGT'ACT
Cuts at: 1675 4742 1675
Size: 3067 2158
SfcI C'TryA_G
Cuts at: 33 1188 1464 1573 2038 2086 2268 2953 3946
Size: 1155 276 109 465 48 182 685 993
Cuts at: 3946 4137 33
Size: 191 1121
Fragments arranged by size:
1155 1121 993 685 465 276 191 182
109 48
SfoI GGC'GCC
Cuts at: 2069 2657 2905 2069
Size: 588 248 4389
Fragments arranged by size:
4389 588 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
SmaI CCC'GGG
Cuts at: 136 1507 2247 136
Size: 1371 740 3114
Fragments arranged by size:
3114 1371 740
SmlI C'TyrA_G
Cuts at: 399 1255 1822 2380 3295 3787 4049 399
Size: 856 567 558 915 492 262 1575
Fragments arranged by size:
1575 915 856 567 558 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5225
SphI G_CATG'C
Cuts at: 247 2278 2778 247
Size: 2031 500 2694
Fragments arranged by size:
2694 2031 500
StyI C'CwwG_G
Cuts at: 107 1097 1166 1796 2165 2359 2713 107
Size: 990 69 630 369 194 354 2619
Fragments arranged by size:
2619 990 630 369 354 194 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 528 2808 2941 3400 3609 528
Size: 2280 133 459 209 2144
Fragments arranged by size:
2280 2144 459 209 133
TatI w'GTAC_w
Cuts at: 1313 1673 4740 1313
Size: 360 3067 1798
Fragments arranged by size:
3067 1798 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1110 1125 1157 4742 4774 1078
Size: 32 15 32 3585 32 1529
Fragments arranged by size:
3585 1529 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 3022 3022
Size: 5225
XbaI T'CTAG_A
Cuts at: 2256 2256
Size: 5225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1173 1359 1618 2720 1173
Size: 186 259 1102 3678
Fragments arranged by size:
3678 1102 259 186
XmaI C'CCGG_G
Cuts at: 134 1505 2245 134
Size: 1371 740 3114
Fragments arranged by size:
3114 1371 740
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsrGI BssHII
BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI