Restriction map for
pF1KB8011
(Circular) (Six-base) MAPSORT of: pF1KB8011.seq Check: 9661 from: 1 to: 4355
pF1KB8011 4355 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 921 2180 2612 3359 3627 710
Size: 211 1259 432 747 268 1438
Fragments arranged by size:
1438 1259 747 432 268 211
AfeI AGC'GCT
Cuts at: 116 116
Size: 4355
AflIII A'CryG_T
Cuts at: 555 1707 2811 3788 555
Size: 1152 1104 977 1122
Fragments arranged by size:
1152 1122 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AleI CACnn'nnGTG
Cuts at: 1183 1183
Size: 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 701 701
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 862 1660 3227 862
Size: 798 1567 1990
Fragments arranged by size:
1990 1567 798
ApaLI G'TGCA_C
Cuts at: 551 3125 551
Size: 2574 1781
ApoI r'AATT_y
Cuts at: 452 1359 3708 3964 452
Size: 907 2349 256 843
Fragments arranged by size:
2349 907 843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 1371 1785 2033 2068 1371
Size: 414 248 35 3658
Fragments arranged by size:
3658 414 248 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 235 235
Size: 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1050 1084 1050
Size: 34 4321
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglI GCCn_nnn'nGGC
Cuts at: 1346 1346
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 855 1306 1467 855
Size: 451 161 3743
Fragments arranged by size:
3743 451 161
Bme1580I G_kGCm'C
Cuts at: 555 815 1980 2073 3129 555
Size: 260 1165 93 1056 1781
Fragments arranged by size:
1781 1165 1056 260 93
BmgBI CAC'GTC
Cuts at: 1058 1058
Size: 4355
BmrI ACTGGGnnnn_n'
Cuts at: 1567 1648 1983 4127 1567
Size: 81 335 2144 1795
Fragments arranged by size:
2144 1795 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 518 780 802 820 1231 442
Size: 76 262 22 18 411 3566
Fragments arranged by size:
3566 411 262 76 22 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 778 885 4059 778
Size: 107 3174 1074
Fragments arranged by size:
3174 1074 107
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 395 1869 3906 6
Size: 389 1474 2037 455
Fragments arranged by size:
2037 1474 455 389
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 238 823 873 190
Size: 48 585 50 3672
Fragments arranged by size:
3672 585 50 48
BseYI C'CCAG_C
Cuts at: 170 197 1780 2538 3115 3611 170
Size: 27 1583 758 577 496 914
Fragments arranged by size:
1583 914 758 577 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1012 697
Size: 315 4040
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 555 1369 2147 2337 3129 555
Size: 814 778 190 792 1781
Fragments arranged by size:
1781 814 792 778 190
BsmI GAATG_Cn'
Cuts at: 694 694
Size: 4355
BsmBI CGTCTCn'nnnn_
Cuts at: 1276 1276
Size: 4355
Bsp1286I G_dGCh'C
Cuts at: 555 815 1369 1980 2073 2147 2337 2399 3129
Size: 260 554 611 93 74 190 62 730
Cuts at: 3129 555
Size: 1781
Fragments arranged by size:
1781 730 611 554 260 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 854 2647 2701 4185 4350 50
Size: 804 1793 54 1484 165 55
Fragments arranged by size:
1793 1484 804 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 2353 2534 2743 2353
Size: 181 209 3965
Fragments arranged by size:
3965 209 181
BsrGI T'GTAC_A
Cuts at: 1118 1118
Size: 4355
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 748 1732 748
Size: 984 3371
BstBI TT'CG_AA
Cuts at: 521 2716 521
Size: 2195 2160
BstXI CCAn_nnnn'nTGG
Cuts at: 293 363 447 293
Size: 70 84 4201
Fragments arranged by size:
4201 84 70
BstYI r'GATC_y
Cuts at: 1 1380 1847 1870 2205 2451 3463 3549 3561
Size: 1379 467 23 335 246 1012 86 12
Cuts at: 3561 3907 1
Size: 346 449
Fragments arranged by size:
1379 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 446 546 107
Size: 339 100 3916
Fragments arranged by size:
3916 339 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 988 1087 2305 2486 2649 988
Size: 99 1218 181 163 2694
Fragments arranged by size:
2694 1218 181 163 99
BtsI GCAGTG_nn'
Cuts at: 440 605 1073 1123 1598 440
Size: 165 468 50 475 3197
Fragments arranged by size:
3197 475 468 165 50
ClaI AT'CG_AT
Cuts at: 897 897
Size: 4355
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3877 1940
Size: 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 722 1274 2378 2588 722
Size: 552 1104 210 2489
Fragments arranged by size:
2489 1104 552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 518 710 780 802 820 921 1231 2180
Size: 76 192 70 22 18 101 310 949
Cuts at: 2180 2612 3359 3627 442
Size: 432 747 268 1170
Fragments arranged by size:
1170 949 747 432 310 268 192 101
76 70 22 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1140 1140
Size: 4355
EcoO109I rG'GnC_Cy
Cuts at: 1494 1494
Size: 4355
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1348 2374 1348
Size: 1026 3329
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 118 1306 1789 2037 3059 4038 118
Size: 1188 483 248 1022 979 435
Fragments arranged by size:
1188 1022 979 483 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2197 2227 2229 2259 2197
Size: 30 2 30 4293
Fragments arranged by size:
4293 30 30 2
HincII GTy'rAC
Cuts at: 336 1394 336
Size: 1058 3297
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 976 1043 1118 3026 3210 424
Size: 552 67 75 1908 184 1569
Fragments arranged by size:
1908 1569 552 184 75 67
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 291 445 1183 2344 2471 291
Size: 154 738 1161 127 2175
Fragments arranged by size:
2175 1161 738 154 127
MspA1I CmG'CkG
Cuts at: 1109 1250 1466 1780 2140 3153 3398 1109
Size: 141 216 314 360 1013 245 2066
Fragments arranged by size:
2066 1013 360 314 245 216 141
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 107 446 546 107
Size: 339 100 3916
Fragments arranged by size:
3916 339 100
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NspI r_CATG'y
Cuts at: 559 1257 1408 1908 2815 559
Size: 698 151 500 907 2099
Fragments arranged by size:
2099 907 698 500 151
PciI A'CATG_T
Cuts at: 555 2811 555
Size: 2256 2099
PflMI CCAn_nnn'nTGG
Cuts at: 253 253
Size: 4355
PfoI T'CCnGG_A
Cuts at: 836 1074 836
Size: 238 4117
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PshAI GACnn'nnGTC
Cuts at: 938 938
Size: 4355
PsiI TTA'TAA
Cuts at: 986 986
Size: 4355
PstI C_TGCA'G
Cuts at: 574 1402 2087 574
Size: 828 685 2842
Fragments arranged by size:
2842 828 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1109 1780 2140 1109
Size: 671 360 3324
Fragments arranged by size:
3324 671 360
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 574 1402 574
Size: 828 3527
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 570 1398 2083 3076 3267 33
Size: 537 828 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 537 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1257 1408 1908 1257
Size: 151 500 3704
Fragments arranged by size:
3704 500 151
StuI AGG'CCT
Cuts at: 1146 1146
Size: 4355
StyI C'CwwG_G
Cuts at: 107 188 446 546 632 920 1136 1489 1843
Size: 81 258 100 86 288 216 353 354
Cuts at: 1843 107
Size: 2619
Fragments arranged by size:
2619 354 353 288 258 216 100 86
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1104 1938 2071 2530 2739 1104
Size: 834 133 459 209 2720
Fragments arranged by size:
2720 834 459 209 133
TatI w'GTAC_w
Cuts at: 1088 1118 1187 1229 3870 1088
Size: 30 69 42 2641 1573
Fragments arranged by size:
2641 1573 69 42 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 3872 3904 832
Size: 32 3008 32 1283
Fragments arranged by size:
3008 1283 32 32
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 777 1330 1850 777
Size: 553 520 3282
Fragments arranged by size:
3282 553 520
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI