Restriction map for
pF1KB8020
(Circular) (Six-base) MAPSORT of: pF1KB8020.seq Check: 633 from: 1 to: 5183
pF1KB8020 5183 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 2221 2221
Size: 5183
Acc65I G'GTAC_C
Cuts at: 622 2199 622
Size: 1577 3606
AclI AA'CG_TT
Cuts at: 483 5023 483
Size: 4540 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 943 1312 3008 3440 4187 4455 443
Size: 500 369 1696 432 747 268 1171
Fragments arranged by size:
1696 1171 747 500 432 369 268
AfeI AGC'GCT
Cuts at: 750 750
Size: 5183
AflIII A'CryG_T
Cuts at: 2535 3639 4616 2535
Size: 1104 977 3102
Fragments arranged by size:
3102 1104 977
AgeI A'CCGG_T
Cuts at: 3571 3571
Size: 5183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1051 1051
Size: 5183
AlwNI CAG_nnn'CTG
Cuts at: 667 2488 4055 667
Size: 1821 1567 1795
Fragments arranged by size:
1821 1795 1567
ApaI G_GGCC'C
Cuts at: 602 602
Size: 5183
ApaLI G'TGCA_C
Cuts at: 3953 3953
Size: 5183
ApoI r'AATT_y
Cuts at: 812 1350 2187 4536 4792 812
Size: 538 837 2349 256 1203
Fragments arranged by size:
2349 1203 837 538 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
AvaI C'yCGr_G
Cuts at: 1004 2203 1004
Size: 1199 3984
BamHI G'GATC_C
Cuts at: 2208 2208
Size: 5183
BanI G'GyrC_C
Cuts at: 622 2199 2613 2861 2896 622
Size: 1577 414 248 35 2909
Fragments arranged by size:
2909 1577 414 248 35
BanII G_rGCy'C
Cuts at: 602 2197 3227 602
Size: 1595 1030 2558
Fragments arranged by size:
2558 1595 1030
BbeI G_GCGC'C
Cuts at: 2617 2865 2617
Size: 248 4935
BciVI GTATCCnnnnn_n'
Cuts at: 3081 3842 5183 3081
Size: 761 1341 3081
Fragments arranged by size:
3081 1341 761
BclI T'GATC_A
Cuts at: 2703 2703
Size: 5183
BglII A'GATC_T
Cuts at: 2698 4735 2698
Size: 2037 3146
BlpI GC'TnA_GC
Cuts at: 1249 2295 1249
Size: 1046 4137
Bme1580I G_kGCm'C
Cuts at: 602 1286 2808 2901 3957 602
Size: 684 1522 93 1056 1828
Fragments arranged by size:
1828 1522 1056 684 93
BmrI ACTGGGnnnn_n'
Cuts at: 2395 2476 2811 4955 2395
Size: 81 335 2144 2623
Fragments arranged by size:
2623 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 542 568 617 773 965 514
Size: 28 26 49 156 192 4732
Fragments arranged by size:
4732 192 156 49 28 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1786 2359 3238 3730 4028 557
Size: 1229 573 879 492 298 1712
Fragments arranged by size:
1712 1229 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 941 4887 941
Size: 3946 1237
BsaAI yAC'GTr
Cuts at: 2536 2536
Size: 5183
BsaBI GATnn'nnATC
Cuts at: 6 2697 4734 6
Size: 2691 2037 455
Fragments arranged by size:
2691 2037 455
BsaHI Gr'CG_yC
Cuts at: 2614 2862 3564 5060 2614
Size: 248 702 1496 2737
Fragments arranged by size:
2737 1496 702 248
BsaWI w'CCGG_w
Cuts at: 368 1305 2372 2893 3571 3845 3992 4403 368
Size: 937 1067 521 678 274 147 411 1148
Fragments arranged by size:
1148 1067 937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 615 645 1331 1361 615
Size: 30 686 30 4437
Fragments arranged by size:
4437 686 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 144 727 757 1798 1819 141
Size: 3 583 30 1041 21 3505
Fragments arranged by size:
3505 1041 583 30 21 3
BseYI C'CCAG_C
Cuts at: 1019 1379 1558 1882 2608 3366 3943 4439 1019
Size: 360 179 324 726 758 577 496 1763
Fragments arranged by size:
1763 758 726 577 496 360 324 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2087 2087
Size: 5183
BsiEI CG_ry'CG
Cuts at: 104 2725 2771 3979 104
Size: 2621 46 1208 1308
Fragments arranged by size:
2621 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1080 2197 2975 3165 3957 1080
Size: 1117 778 190 792 2306
Fragments arranged by size:
2306 1117 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 698 920 698
Size: 222 4961
Bsp1286I G_dGCh'C
Cuts at: 602 1080 1286 2197 2808 2901 2975 3165 3227
Size: 478 206 911 611 93 74 190 62
Cuts at: 3227 3957 602
Size: 730 1828
Fragments arranged by size:
1828 911 730 611 478 206 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1305 4403 1305
Size: 3098 2085
BspHI T'CATG_A
Cuts at: 4359 4359
Size: 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 2233 2447 2749 3130 610
Size: 1623 214 302 381 2663
Fragments arranged by size:
2663 1623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 675 3475 3529 5013 5178 50
Size: 625 2800 54 1484 165 55
Fragments arranged by size:
2800 1484 625 165 55 54
BsrDI GCAATG_nn'
Cuts at: 917 990 1263 1724 3095 917
Size: 73 273 461 1371 3005
Fragments arranged by size:
3005 1371 461 273 73
BsrFI r'CCGG_y
Cuts at: 3181 3362 3571 3181
Size: 181 209 4793
Fragments arranged by size:
4793 209 181
BsrGI T'GTAC_A
Cuts at: 218 218
Size: 5183
BssSI C'ACGA_G
Cuts at: 1106 1631 3454 3812 1106
Size: 525 1823 358 2477
Fragments arranged by size:
2477 1823 525 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2560 2560
Size: 5183
BstBI TT'CG_AA
Cuts at: 3544 3544
Size: 5183
BstYI r'GATC_y
Cuts at: 1 612 1476 2208 2675 2698 3033 3279 4291
Size: 611 864 732 467 23 335 246 1012
Cuts at: 4291 4377 4389 4735 1
Size: 86 12 346 449
Fragments arranged by size:
1012 864 732 611 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1617 2084 107
Size: 1510 467 3206
Fragments arranged by size:
3206 1510 467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3133 3314 3477 3133
Size: 181 163 4839
Fragments arranged by size:
4839 181 163
BtsI GCAGTG_nn'
Cuts at: 668 1033 2426 668
Size: 365 1393 3425
Fragments arranged by size:
3425 1393 365
DraI TTT'AAA
Cuts at: 2182 2182
Size: 5183
DraIII CAC_nnn'GTG
Cuts at: 301 301
Size: 5183
DrdI GACnn_nn'nnGTC
Cuts at: 690 2889 3747 690
Size: 2199 858 2126
Fragments arranged by size:
2199 2126 858
EaeI y'GGCC_r
Cuts at: 186 1271 2063 2768 2942 3333 3360 4705 186
Size: 1085 792 705 174 391 27 1345 664
Fragments arranged by size:
1345 1085 792 705 664 391 174 27
EagI C'GGCC_G
Cuts at: 2768 2768
Size: 5183
EarI CTCTTCn'nnn_
Cuts at: 162 701 1106 1136 1961 3206 3416 162
Size: 539 405 30 825 1245 210 1929
Fragments arranged by size:
1929 1245 825 539 405 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3391 3701 3847 4993 3391
Size: 310 146 1146 3581
Fragments arranged by size:
3581 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2195 2195
Size: 5183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 514 542 568 617 773 943 965 1312
Size: 71 28 26 49 156 170 22 347
Cuts at: 1312 3008 3440 4187 4455 443
Size: 1696 432 747 268 1171
Fragments arranged by size:
1696 1171 747 432 347 268 170 156
71 49 28 26 22
EcoO109I rG'GnC_Cy
Cuts at: 2322 2322
Size: 5183
EcoRI G'AATT_C
Cuts at: 2187 2187
Size: 5183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1147 3202 1147
Size: 2055 3128
FspI TGC'GCA
Cuts at: 2964 2964
Size: 5183
HaeII r_GCGC'y
Cuts at: 752 1065 2617 2865 3887 4866 752
Size: 313 1552 248 1022 979 1069
Fragments arranged by size:
1552 1069 1022 979 313 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 1084 1116 3025 3055 3057 3087 118
Size: 32 934 32 1909 30 2 30 2214
Fragments arranged by size:
2214 1909 934 32 32 30 30 2
HincII GTy'rAC
Cuts at: 966 2081 2222 966
Size: 1115 141 3927
Fragments arranged by size:
3927 1115 141
KasI G'GCGC_C
Cuts at: 2613 2861 2613
Size: 248 4935
KpnI G_GTAC'C
Cuts at: 626 2203 626
Size: 1577 3606
MfeI C'AATT_G
Cuts at: 1358 1358
Size: 5183
MluI A'CGCG_T
Cuts at: 4616 4616
Size: 5183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 1196 3854 4038 344
Size: 852 2658 184 1489
Fragments arranged by size:
2658 1489 852 184
MscI TGG'CCA
Cuts at: 188 1273 2944 188
Size: 1085 1671 2427
Fragments arranged by size:
2427 1671 1085
MslI CAynn'nnrTG
Cuts at: 998 1597 2059 3172 3299 998
Size: 599 462 1113 127 2882
Fragments arranged by size:
2882 1113 599 462 127
MspA1I CmG'CkG
Cuts at: 2294 2608 2968 3981 4226 2294
Size: 314 360 1013 245 3251
Fragments arranged by size:
3251 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3364 3364
Size: 5183
NarI GG'CG_CC
Cuts at: 2614 2862 2614
Size: 248 4935
NcoI C'CATG_G
Cuts at: 107 1617 107
Size: 1510 3673
NgoMIV G'CCGG_C
Cuts at: 3362 3362
Size: 5183
NspI r_CATG'y
Cuts at: 1499 2236 2736 3643 1499
Size: 737 500 907 3039
Fragments arranged by size:
3039 907 737 500
PciI A'CATG_T
Cuts at: 3639 3639
Size: 5183
PflMI CCAn_nnn'nTGG
Cuts at: 1618 1618
Size: 5183
PfoI T'CCnGG_A
Cuts at: 519 893 519
Size: 374 4809
PmeI GTTT'AAAC
Cuts at: 2182 2182
Size: 5183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 4322
Size: 32 5151
PspOMI G'GGCC_C
Cuts at: 598 598
Size: 5183
PstI C_TGCA'G
Cuts at: 284 621 2230 2915 284
Size: 337 1609 685 2552
Fragments arranged by size:
2552 1609 685 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5183
PvuII CAG'CTG
Cuts at: 2608 2968 2608
Size: 360 4823
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 5183
SacI G_AGCT'C
Cuts at: 2197 2197
Size: 5183
SalI G'TCGA_C
Cuts at: 2220 2220
Size: 5183
SapI GCTCTTCn'nnn_
Cuts at: 1136 3206 3416 1136
Size: 2070 210 2903
Fragments arranged by size:
2903 2070 210
SbfI CC_TGCA'GG
Cuts at: 621 2230 621
Size: 1609 3574
ScaI AGT'ACT
Cuts at: 1818 4700 1818
Size: 2882 2301
SfcI C'TryA_G
Cuts at: 33 280 617 2226 2911 3904 4095 33
Size: 247 337 1609 685 993 191 1121
Fragments arranged by size:
1609 1121 993 685 337 247 191
SfoI GGC'GCC
Cuts at: 2615 2863 2615
Size: 248 4935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
SmaI CCC'GGG
Cuts at: 2205 2205
Size: 5183
SmlI C'TyrA_G
Cuts at: 572 1765 2338 3253 3745 4007 572
Size: 1193 573 915 492 262 1748
Fragments arranged by size:
1748 1193 915 573 492 262
SpeI A'CTAG_T
Cuts at: 67 562 2106 67
Size: 495 1544 3144
Fragments arranged by size:
3144 1544 495
SphI G_CATG'C
Cuts at: 2236 2736 2236
Size: 500 4683
StyI C'CwwG_G
Cuts at: 107 1542 1617 2317 2671 107
Size: 1435 75 700 354 2619
Fragments arranged by size:
2619 1435 700 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 809 847 1277 2766 2899 3358 3567 809
Size: 38 430 1489 133 459 209 2425
Fragments arranged by size:
2425 1489 459 430 209 133 38
TatI w'GTAC_w
Cuts at: 218 1816 4698 218
Size: 1598 2882 703
Fragments arranged by size:
2882 1598 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1331 1363 1754 1786 4700 4732 1331
Size: 32 391 32 2914 32 1782
Fragments arranged by size:
2914 1782 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 250 2980 250
Size: 2730 2453
XbaI T'CTAG_A
Cuts at: 2214 2214
Size: 5183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2678 2678
Size: 5183
XmaI C'CCGG_G
Cuts at: 2203 2203
Size: 5183
XmnI GAAnn'nnTTC
Cuts at: 352 352
Size: 5183
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI