(Circular) (Six-base) MAPSORT of: pF1KB8031.seq Check: 1995 from: 1 to: 4871 pF1KB8031 4871 bp With 182 enzymes: SgfI * May 21, 2013 16:22 .. AccI GT'mk_AC Cuts at: 1909 1909 Size: 4871 Acc65I G'GTAC_C Cuts at: 1887 1887 Size: 4871 AclI AA'CG_TT Cuts at: 4711 4711 Size: 4871 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1064 1698 2696 3128 3875 4143 1064 Size: 634 998 432 747 268 1792 Fragments arranged by size: 1792 998 747 634 432 268 AflIII A'CryG_T Cuts at: 1058 2223 3327 4304 1058 Size: 1165 1104 977 1625 Fragments arranged by size: 1625 1165 1104 977 AgeI A'CCGG_T Cuts at: 3259 3259 Size: 4871 AlwNI CAG_nnn'CTG Cuts at: 492 837 885 2176 3743 492 Size: 345 48 1291 1567 1620 Fragments arranged by size: 1620 1567 1291 345 48 ApaI G_GGCC'C Cuts at: 513 513 Size: 4871 ApaLI G'TGCA_C Cuts at: 482 3641 482 Size: 3159 1712 ApoI r'AATT_y Cuts at: 1875 4224 4480 1875 Size: 2349 256 2266 Fragments arranged by size: 2349 2266 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4871 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4871 AvaI C'yCGr_G Cuts at: 113 434 462 529 901 1154 1415 1891 113 Size: 321 28 67 372 253 261 476 3093 Fragments arranged by size: 3093 476 372 321 261 253 67 28 BamHI G'GATC_C Cuts at: 1896 1896 Size: 4871 BanI G'GyrC_C Cuts at: 444 525 924 973 1426 1521 1887 2301 2549 Size: 81 399 49 453 95 366 414 248 Cuts at: 2549 2584 444 Size: 35 2731 Fragments arranged by size: 2731 453 414 399 366 248 95 81 49 35 BanII G_rGCy'C Cuts at: 304 513 539 1391 1470 1589 1709 1885 2915 Size: 209 26 852 79 119 120 176 1030 Cuts at: 2915 304 Size: 2260 Fragments arranged by size: 2260 1030 852 209 176 120 119 79 26 BbeI G_GCGC'C Cuts at: 2305 2553 2305 Size: 248 4623 BbsI GAAGACnn'nnnn_ Cuts at: 801 801 Size: 4871 BbvCI CC'TCA_GC Cuts at: 646 646 Size: 4871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 327 361 327 Size: 34 4837 BciVI GTATCCnnnnn_n' Cuts at: 2769 3530 4871 2769 Size: 761 1341 2769 Fragments arranged by size: 2769 1341 761 BclI T'GATC_A Cuts at: 1535 2391 1535 Size: 856 4015 BglI GCCn_nnn'nGGC Cuts at: 569 569 Size: 4871 BglII A'GATC_T Cuts at: 2386 4423 2386 Size: 2037 2834 BlpI GC'TnA_GC Cuts at: 1225 1983 1225 Size: 758 4113 Bme1580I G_kGCm'C Cuts at: 486 505 513 530 929 1526 2496 2589 3645 Size: 19 8 17 399 597 970 93 1056 Cuts at: 3645 486 Size: 1712 Fragments arranged by size: 1712 1056 970 597 399 93 19 17 8 BmgBI CAC'GTC Cuts at: 1271 1271 Size: 4871 BmrI ACTGGGnnnn_n' Cuts at: 507 508 1483 2083 2164 2499 4643 507 Size: 1 975 600 81 335 2144 735 Fragments arranged by size: 2144 975 735 600 335 81 1 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 304 3238 304 Size: 2934 1937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 274 799 940 1126 274 Size: 525 141 186 4019 Fragments arranged by size: 4019 525 186 141 Bpu10I CC'TnA_GC Cuts at: 646 646 Size: 4871 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1069 2047 2926 3418 3716 1069 Size: 978 879 492 298 2224 Fragments arranged by size: 2224 978 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 775 1165 4575 775 Size: 390 3410 1071 Fragments arranged by size: 3410 1071 390 BsaAI yAC'GTr Cuts at: 2224 2224 Size: 4871 BsaBI GATnn'nnATC Cuts at: 6 2385 4422 6 Size: 2379 2037 455 Fragments arranged by size: 2379 2037 455 BsaHI Gr'CG_yC Cuts at: 691 2302 2550 3252 4748 691 Size: 1611 248 702 1496 814 Fragments arranged by size: 1611 1496 814 702 248 BsaWI w'CCGG_w Cuts at: 2060 2581 3259 3533 3680 4091 2060 Size: 521 678 274 147 411 2840 Fragments arranged by size: 2840 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1334 1364 1442 1472 1334 Size: 30 78 30 4733 Fragments arranged by size: 4733 78 30 30 BseYI C'CCAG_C Cuts at: 147 825 1113 1164 1181 1287 2296 3054 3631 Size: 678 288 51 17 106 1009 758 577 Cuts at: 3631 4127 147 Size: 496 891 Fragments arranged by size: 1009 891 758 678 577 496 288 106 51 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 321 643 746 1747 321 Size: 322 103 1001 3445 Fragments arranged by size: 3445 1001 322 103 BsiEI CG_ry'CG Cuts at: 104 2413 2459 3667 104 Size: 2309 46 1208 1308 Fragments arranged by size: 2309 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 486 539 1391 1470 1589 1885 2663 2853 3645 Size: 53 852 79 119 296 778 190 792 Cuts at: 3645 486 Size: 1712 Fragments arranged by size: 1712 852 792 778 296 190 119 79 53 BsmI GAATG_Cn' Cuts at: 1152 1152 Size: 4871 BsmBI CGTCTCn'nnnn_ Cuts at: 433 1072 1833 433 Size: 639 761 3471 Fragments arranged by size: 3471 761 639 Bsp1286I G_dGCh'C Cuts at: 304 486 505 513 530 539 929 1391 1470 Size: 182 19 8 17 9 390 462 79 Cuts at: 1470 1526 1589 1709 1885 2496 2589 2663 2853 Size: 56 63 120 176 611 93 74 190 Cuts at: 2853 2915 3645 304 Size: 62 730 1530 Fragments arranged by size: 1530 730 611 462 390 190 182 176 120 93 79 74 63 62 56 19 17 9 8 BspEI T'CCGG_A Cuts at: 4091 4091 Size: 4871 BspHI T'CATG_A Cuts at: 1616 4047 1616 Size: 2431 2440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1169 1921 2135 2437 2818 1169 Size: 752 214 302 381 3222 Fragments arranged by size: 3222 752 381 302 214 BsrBI CCG'CTC Cuts at: 50 1651 3163 3217 4701 4866 50 Size: 1601 1512 54 1484 165 55 Fragments arranged by size: 1601 1512 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 848 1655 2783 848 Size: 807 1128 2936 Fragments arranged by size: 2936 1128 807 BsrFI r'CCGG_y Cuts at: 447 2869 3050 3259 447 Size: 2422 181 209 2059 Fragments arranged by size: 2422 2059 209 181 BssSI C'ACGA_G Cuts at: 3142 3500 3142 Size: 358 4513 BstAPI GCAn_nnn'nTGC Cuts at: 973 2248 973 Size: 1275 3596 BstBI TT'CG_AA Cuts at: 3232 3232 Size: 4871 BstXI CCAn_nnnn'nTGG Cuts at: 755 777 1747 755 Size: 22 970 3879 Fragments arranged by size: 3879 970 22 BstYI r'GATC_y Cuts at: 1 1896 2363 2386 2721 2967 3979 4065 4077 Size: 1895 467 23 335 246 1012 86 12 Cuts at: 4077 4423 1 Size: 346 449 Fragments arranged by size: 1895 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 733 740 1837 733 Size: 7 1097 3767 Fragments arranged by size: 3767 1097 7 BtgI C'CryG_G Cuts at: 107 128 308 540 567 754 785 1257 1307 Size: 21 180 232 27 187 31 472 50 Cuts at: 1307 1360 1517 1746 107 Size: 53 157 229 3232 Fragments arranged by size: 3232 472 232 229 187 180 157 53 50 31 27 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 306 591 1360 1422 2821 3002 3165 306 Size: 285 769 62 1399 181 163 2012 Fragments arranged by size: 2012 1399 769 285 181 163 62 BtsI GCAGTG_nn' Cuts at: 2114 2114 Size: 4871 ClaI AT'CG_AT Cuts at: 1202 1202 Size: 4871 DraI TTT'AAA Cuts at: 1870 1870 Size: 4871 DrdI GACnn_nn'nnGTC Cuts at: 259 1269 2577 3435 259 Size: 1010 1308 858 1695 Fragments arranged by size: 1695 1308 1010 858 EaeI y'GGCC_r Cuts at: 449 570 767 788 887 1034 1206 1241 1652 Size: 121 197 21 99 147 172 35 411 Cuts at: 1652 1749 2456 2630 3021 3048 4393 449 Size: 97 707 174 391 27 1345 927 Fragments arranged by size: 1345 927 707 411 391 197 174 172 147 121 99 97 35 27 21 EagI C'GGCC_G Cuts at: 2456 2456 Size: 4871 EarI CTCTTCn'nnn_ Cuts at: 715 1837 2894 3104 715 Size: 1122 1057 210 2482 Fragments arranged by size: 2482 1122 1057 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 389 1180 3079 3389 3535 4681 389 Size: 791 1899 310 146 1146 579 Fragments arranged by size: 1899 1146 791 579 310 146 EcoICRI GAG'CTC Cuts at: 537 1389 1468 1587 1883 537 Size: 852 79 119 296 3525 Fragments arranged by size: 3525 852 296 119 79 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 274 799 940 1064 1126 1698 2696 3128 3875 Size: 525 141 124 62 572 998 432 747 Cuts at: 3875 4143 274 Size: 268 1002 Fragments arranged by size: 1002 998 747 572 525 432 268 141 124 62 EcoO109I rG'GnC_Cy Cuts at: 207 509 744 882 1119 1158 1769 1811 2010 Size: 302 235 138 237 39 611 42 199 Cuts at: 2010 207 Size: 3068 Fragments arranged by size: 3068 611 302 237 235 199 138 42 39 EcoRI G'AATT_C Cuts at: 1875 1875 Size: 4871 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 4871 FspI TGC'GCA Cuts at: 2652 2652 Size: 4871 HaeII r_GCGC'y Cuts at: 166 1307 2305 2553 3575 4554 166 Size: 1141 998 248 1022 979 483 Fragments arranged by size: 1141 1022 998 979 483 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 269 301 368 400 2713 2743 2745 2775 269 Size: 32 67 32 2313 30 2 30 2365 Fragments arranged by size: 2365 2313 67 32 32 30 30 2 HincII GTy'rAC Cuts at: 351 695 1680 1910 351 Size: 344 985 230 3312 Fragments arranged by size: 3312 985 344 230 KasI G'GCGC_C Cuts at: 2301 2549 2301 Size: 248 4623 KpnI G_GTAC'C Cuts at: 1891 1891 Size: 4871 MluI A'CGCG_T Cuts at: 4304 4304 Size: 4871 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3542 3726 3542 Size: 184 4687 MscI TGG'CCA Cuts at: 769 790 889 1036 1751 2632 769 Size: 21 99 147 715 881 3008 Fragments arranged by size: 3008 881 715 147 99 21 MslI CAynn'nnrTG Cuts at: 753 2860 2987 753 Size: 2107 127 2637 Fragments arranged by size: 2637 2107 127 MspA1I CmG'CkG Cuts at: 591 870 1982 2296 2656 3669 3914 591 Size: 279 1112 314 360 1013 245 1548 Fragments arranged by size: 1548 1112 1013 360 314 279 245 NaeI GCC'GGC Cuts at: 449 3052 449 Size: 2603 2268 NarI GG'CG_CC Cuts at: 2302 2550 2302 Size: 248 4623 NcoI C'CATG_G Cuts at: 107 567 754 1517 107 Size: 460 187 763 3461 Fragments arranged by size: 3461 763 460 187 NgoMIV G'CCGG_C Cuts at: 447 3050 447 Size: 2603 2268 NspI r_CATG'y Cuts at: 631 1678 1924 2424 3331 631 Size: 1047 246 500 907 2171 Fragments arranged by size: 2171 1047 907 500 246 PasI CC'CwG_GG Cuts at: 581 930 1548 581 Size: 349 618 3904 Fragments arranged by size: 3904 618 349 PciI A'CATG_T Cuts at: 3327 3327 Size: 4871 PflMI CCAn_nnn'nTGG Cuts at: 837 837 Size: 4871 PfoI T'CCnGG_A Cuts at: 1391 1470 1391 Size: 79 4792 PmeI GTTT'AAAC Cuts at: 1870 1870 Size: 4871 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 4010 Size: 32 4839 PpuMI rG'GwC_Cy Cuts at: 744 882 1119 1158 744 Size: 138 237 39 4457 Fragments arranged by size: 4457 237 138 39 PspOMI G'GGCC_C Cuts at: 509 509 Size: 4871 PstI C_TGCA'G Cuts at: 881 969 1918 2603 881 Size: 88 949 685 3149 Fragments arranged by size: 3149 949 685 88 PvuI CG_AT'CG Cuts at: 104 104 Size: 4871 PvuII CAG'CTG Cuts at: 591 870 2296 2656 591 Size: 279 1426 360 2806 Fragments arranged by size: 2806 1426 360 279 RsrII CG'GwC_CG Cuts at: 3066 3066 Size: 4871 SacI G_AGCT'C Cuts at: 539 1391 1470 1589 1885 539 Size: 852 79 119 296 3525 Fragments arranged by size: 3525 852 296 119 79 SalI G'TCGA_C Cuts at: 1908 1908 Size: 4871 SanDI GG'GwC_CC Cuts at: 1119 1119 Size: 4871 SapI GCTCTTCn'nnn_ Cuts at: 715 2894 3104 715 Size: 2179 210 2482 Fragments arranged by size: 2482 2179 210 SbfI CC_TGCA'GG Cuts at: 969 1918 969 Size: 949 3922 ScaI AGT'ACT Cuts at: 4388 4388 Size: 4871 SfcI C'TryA_G Cuts at: 33 877 965 1233 1914 2599 3592 3783 33 Size: 844 88 268 681 685 993 191 1121 Fragments arranged by size: 1121 993 844 685 681 268 191 88 SfoI GGC'GCC Cuts at: 2303 2551 2303 Size: 248 4623 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4871 SmaI CCC'GGG Cuts at: 115 436 531 1156 1417 1893 115 Size: 321 95 625 261 476 3093 Fragments arranged by size: 3093 625 476 321 261 95 SmlI C'TyrA_G Cuts at: 1084 2026 2941 3433 3695 1084 Size: 942 915 492 262 2260 Fragments arranged by size: 2260 942 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4871 SphI G_CATG'C Cuts at: 1924 2424 1924 Size: 500 4371 StuI AGG'CCT Cuts at: 226 738 1041 1249 226 Size: 512 303 208 3848 Fragments arranged by size: 3848 512 303 208 StyI C'CwwG_G Cuts at: 107 257 567 754 854 1094 1517 2005 2359 Size: 150 310 187 100 240 423 488 354 Cuts at: 2359 107 Size: 2619 Fragments arranged by size: 2619 488 423 354 310 240 187 150 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1450 2454 2587 3046 3255 1450 Size: 1004 133 459 209 3066 Fragments arranged by size: 3066 1004 459 209 133 TatI w'GTAC_w Cuts at: 947 4386 947 Size: 3439 1432 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1334 1366 1442 1474 4388 4420 1334 Size: 32 76 32 2914 32 1785 Fragments arranged by size: 2914 1785 76 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2668 2668 Size: 4871 XbaI T'CTAG_A Cuts at: 1902 1902 Size: 4871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 211 327 565 798 1201 2366 211 Size: 116 238 233 403 1165 2716 Fragments arranged by size: 2716 1165 403 238 233 116 XmaI C'CCGG_G Cuts at: 113 434 529 1154 1415 1891 113 Size: 321 95 625 261 476 3093 Fragments arranged by size: 3093 625 476 321 261 95 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BfrBI BmtI BseRI BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI