(Circular) (Six-base) MAPSORT of: pF1KB8106.seq Check: 6825 from: 1 to: 3644 pF1KB8106 3644 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 519 519 Size: 3644 AccI GT'mk_AC Cuts at: 139 311 682 139 Size: 172 371 3101 Fragments arranged by size: 3101 371 172 Acc65I G'GTAC_C Cuts at: 660 660 Size: 3644 AclI AA'CG_TT Cuts at: 3484 3484 Size: 3644 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1469 1901 2648 2916 1469 Size: 432 747 268 2197 Fragments arranged by size: 2197 747 432 268 AflIII A'CryG_T Cuts at: 996 2100 3077 996 Size: 1104 977 1563 Fragments arranged by size: 1563 1104 977 AgeI A'CCGG_T Cuts at: 2032 2032 Size: 3644 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 454 486 454 Size: 32 3612 AlwNI CAG_nnn'CTG Cuts at: 420 597 949 2516 420 Size: 177 352 1567 1548 Fragments arranged by size: 1567 1548 352 177 ApaLI G'TGCA_C Cuts at: 2414 2414 Size: 3644 ApoI r'AATT_y Cuts at: 648 2997 3253 648 Size: 2349 256 1039 Fragments arranged by size: 2349 1039 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3644 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3644 AvaI C'yCGr_G Cuts at: 664 664 Size: 3644 BamHI G'GATC_C Cuts at: 669 669 Size: 3644 BanI G'GyrC_C Cuts at: 660 1074 1322 1357 660 Size: 414 248 35 2947 Fragments arranged by size: 2947 414 248 35 BanII G_rGCy'C Cuts at: 658 1688 658 Size: 1030 2614 BbeI G_GCGC'C Cuts at: 1078 1326 1078 Size: 248 3396 BbvCI CC'TCA_GC Cuts at: 421 421 Size: 3644 BciVI GTATCCnnnnn_n' Cuts at: 1542 2303 3644 1542 Size: 761 1341 1542 Fragments arranged by size: 1542 1341 761 BclI T'GATC_A Cuts at: 1164 1164 Size: 3644 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 3644 BglII A'GATC_T Cuts at: 327 1159 3196 327 Size: 832 2037 775 Fragments arranged by size: 2037 832 775 BlpI GC'TnA_GC Cuts at: 756 756 Size: 3644 Bme1580I G_kGCm'C Cuts at: 1269 1362 2418 1269 Size: 93 1056 2495 Fragments arranged by size: 2495 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 209 856 937 1272 3416 209 Size: 647 81 335 2144 437 Fragments arranged by size: 2144 647 437 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2011 Size: 3644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 472 472 Size: 3644 Bpu10I CC'TnA_GC Cuts at: 421 421 Size: 3644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 820 1699 2191 2489 820 Size: 879 492 298 1975 Fragments arranged by size: 1975 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3348 3348 Size: 3644 BsaAI yAC'GTr Cuts at: 997 997 Size: 3644 BsaBI GATnn'nnATC Cuts at: 6 176 1158 3195 6 Size: 170 982 2037 455 Fragments arranged by size: 2037 982 455 170 BsaHI Gr'CG_yC Cuts at: 1075 1323 2025 3521 1075 Size: 248 702 1496 1198 Fragments arranged by size: 1496 1198 702 248 BsaWI w'CCGG_w Cuts at: 833 1354 2032 2306 2453 2864 833 Size: 521 678 274 147 411 1613 Fragments arranged by size: 1613 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 454 484 454 Size: 30 3614 BseYI C'CCAG_C Cuts at: 524 1069 1827 2404 2900 524 Size: 545 758 577 496 1268 Fragments arranged by size: 1268 758 577 545 496 BsiEI CG_ry'CG Cuts at: 104 1186 1232 2440 104 Size: 1082 46 1208 1308 Fragments arranged by size: 1308 1208 1082 46 BsiHKAI G_wGCw'C Cuts at: 447 658 1436 1626 2418 447 Size: 211 778 190 792 1673 Fragments arranged by size: 1673 792 778 211 190 Bsp1286I G_dGCh'C Cuts at: 447 658 1269 1362 1436 1626 1688 2418 447 Size: 211 611 93 74 190 62 730 1673 Fragments arranged by size: 1673 730 611 211 190 93 74 62 BspEI T'CCGG_A Cuts at: 2864 2864 Size: 3644 BspHI T'CATG_A Cuts at: 2820 2820 Size: 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 519 694 908 1210 1591 519 Size: 175 214 302 381 2572 Fragments arranged by size: 2572 381 302 214 175 BsrBI CCG'CTC Cuts at: 50 1936 1990 3474 3639 50 Size: 1886 54 1484 165 55 Fragments arranged by size: 1886 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1556 1556 Size: 3644 BsrFI r'CCGG_y Cuts at: 1642 1823 2032 1642 Size: 181 209 3254 Fragments arranged by size: 3254 209 181 BssSI C'ACGA_G Cuts at: 1915 2273 1915 Size: 358 3286 BstAPI GCAn_nnn'nTGC Cuts at: 1021 1021 Size: 3644 BstBI TT'CG_AA Cuts at: 2005 2005 Size: 3644 BstYI r'GATC_y Cuts at: 1 327 669 1136 1159 1494 1740 2752 2838 Size: 326 342 467 23 335 246 1012 86 Cuts at: 2838 2850 3196 1 Size: 12 346 449 Fragments arranged by size: 1012 467 449 346 342 335 326 246 86 23 12 BstZ17I GTA'TAC Cuts at: 140 312 140 Size: 172 3472 BtgI C'CryG_G Cuts at: 107 107 Size: 3644 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1594 1775 1938 1594 Size: 181 163 3300 Fragments arranged by size: 3300 181 163 BtsI GCAGTG_nn' Cuts at: 445 887 445 Size: 442 3202 DraI TTT'AAA Cuts at: 643 643 Size: 3644 DraIII CAC_nnn'GTG Cuts at: 543 543 Size: 3644 DrdI GACnn_nn'nnGTC Cuts at: 1350 2208 1350 Size: 858 2786 EaeI y'GGCC_r Cuts at: 1229 1403 1794 1821 3166 1229 Size: 174 391 27 1345 1707 Fragments arranged by size: 1707 1345 391 174 27 EagI C'GGCC_G Cuts at: 1229 1229 Size: 3644 EarI CTCTTCn'nnn_ Cuts at: 403 1667 1877 403 Size: 1264 210 2170 Fragments arranged by size: 2170 1264 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1852 2162 2308 3454 1852 Size: 310 146 1146 2042 Fragments arranged by size: 2042 1146 310 146 EcoICRI GAG'CTC Cuts at: 656 656 Size: 3644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 472 1469 1901 2648 2916 472 Size: 997 432 747 268 1200 Fragments arranged by size: 1200 997 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 783 783 Size: 3644 EcoRI G'AATT_C Cuts at: 648 648 Size: 3644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1663 1663 Size: 3644 FspI TGC'GCA Cuts at: 1425 1425 Size: 3644 HaeII r_GCGC'y Cuts at: 118 1078 1326 2348 3327 118 Size: 960 248 1022 979 435 Fragments arranged by size: 1022 979 960 435 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 491 523 573 605 1486 1516 1518 1548 491 Size: 32 50 32 881 30 2 30 2587 Fragments arranged by size: 2587 881 50 32 32 30 30 2 HincII GTy'rAC Cuts at: 683 683 Size: 3644 KasI G'GCGC_C Cuts at: 1074 1322 1074 Size: 248 3396 KpnI G_GTAC'C Cuts at: 664 664 Size: 3644 MluI A'CGCG_T Cuts at: 3077 3077 Size: 3644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2315 2499 2315 Size: 184 3460 MscI TGG'CCA Cuts at: 1405 1405 Size: 3644 MslI CAynn'nnrTG Cuts at: 1633 1760 1633 Size: 127 3517 MspA1I CmG'CkG Cuts at: 755 1069 1429 2442 2687 755 Size: 314 360 1013 245 1712 Fragments arranged by size: 1712 1013 360 314 245 NaeI GCC'GGC Cuts at: 1825 1825 Size: 3644 NarI GG'CG_CC Cuts at: 1075 1323 1075 Size: 248 3396 NcoI C'CATG_G Cuts at: 107 107 Size: 3644 NgoMIV G'CCGG_C Cuts at: 1823 1823 Size: 3644 NspI r_CATG'y Cuts at: 697 1197 2104 697 Size: 500 907 2237 Fragments arranged by size: 2237 907 500 PciI A'CATG_T Cuts at: 2100 2100 Size: 3644 PflMI CCAn_nnn'nTGG Cuts at: 531 531 Size: 3644 PmeI GTTT'AAAC Cuts at: 643 643 Size: 3644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 454 486 2783 2815 454 Size: 32 2297 32 1283 Fragments arranged by size: 2297 1283 32 32 PstI C_TGCA'G Cuts at: 602 691 1376 602 Size: 89 685 2870 Fragments arranged by size: 2870 685 89 PvuI CG_AT'CG Cuts at: 104 104 Size: 3644 PvuII CAG'CTG Cuts at: 1069 1429 1069 Size: 360 3284 RsrII CG'GwC_CG Cuts at: 1839 1839 Size: 3644 SacI G_AGCT'C Cuts at: 658 658 Size: 3644 SalI G'TCGA_C Cuts at: 681 681 Size: 3644 SapI GCTCTTCn'nnn_ Cuts at: 1667 1877 1667 Size: 210 3434 SbfI CC_TGCA'GG Cuts at: 691 691 Size: 3644 ScaI AGT'ACT Cuts at: 196 3161 196 Size: 2965 679 SfcI C'TryA_G Cuts at: 33 598 687 1372 2365 2556 33 Size: 565 89 685 993 191 1121 Fragments arranged by size: 1121 993 685 565 191 89 SfoI GGC'GCC Cuts at: 1076 1324 1076 Size: 248 3396 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3644 SmaI CCC'GGG Cuts at: 666 666 Size: 3644 SmlI C'TyrA_G Cuts at: 799 1714 2206 2468 799 Size: 915 492 262 1975 Fragments arranged by size: 1975 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3644 SphI G_CATG'C Cuts at: 697 1197 697 Size: 500 3144 StuI AGG'CCT Cuts at: 235 299 235 Size: 64 3580 StyI C'CwwG_G Cuts at: 107 778 1132 107 Size: 671 354 2619 Fragments arranged by size: 2619 671 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1227 1360 1819 2028 1227 Size: 133 459 209 2843 Fragments arranged by size: 2843 459 209 133 TatI w'GTAC_w Cuts at: 194 3159 194 Size: 2965 679 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 261 293 3161 3193 261 Size: 32 2868 32 712 Fragments arranged by size: 2868 712 32 32 Tth111I GACn'n_nGTC Cuts at: 585 1441 585 Size: 856 2788 XbaI T'CTAG_A Cuts at: 675 675 Size: 3644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 159 1139 159 Size: 980 2664 XmaI C'CCGG_G Cuts at: 664 664 Size: 3644 XmnI GAAnn'nnTTC Cuts at: 250 250 Size: 3644 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI