Restriction map for
pF1KB8107
(Circular) (Six-base) MAPSORT of: pF1KB8107.seq Check: 495 from: 1 to: 3779
pF1KB8107 3779 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 817 817
Size: 3779
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 3619 3619
Size: 3779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1604 2036 2783 3051 1604
Size: 432 747 268 2332
Fragments arranged by size:
2332 747 432 268
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 2167 2167
Size: 3779
AleI CACnn'nnGTG
Cuts at: 433 433
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 1084 2651 1084
Size: 1567 2212
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 608 783 3132 3388 608
Size: 175 2349 256 999
Fragments arranged by size:
2349 999 256 175
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 799 799
Size: 3779
BamHI G'GATC_C
Cuts at: 804 804
Size: 3779
BanI G'GyrC_C
Cuts at: 795 1209 1457 1492 795
Size: 414 248 35 3082
Fragments arranged by size:
3082 414 248 35
BanII G_rGCy'C
Cuts at: 793 1823 793
Size: 1030 2749
BbeI G_GCGC'C
Cuts at: 1213 1461 1213
Size: 248 3531
BciVI GTATCCnnnnn_n'
Cuts at: 1677 2438 3779 1677
Size: 761 1341 1677
Fragments arranged by size:
1677 1341 761
BclI T'GATC_A
Cuts at: 308 1299 308
Size: 991 2788
BfrBI ATG'CAT
Cuts at: 343 343
Size: 3779
BglII A'GATC_T
Cuts at: 1294 3331 1294
Size: 2037 1742
BlpI GC'TnA_GC
Cuts at: 891 891
Size: 3779
Bme1580I G_kGCm'C
Cuts at: 1404 1497 2553 1404
Size: 93 1056 2630
Fragments arranged by size:
2630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 644 991 1072 1407 3551 644
Size: 347 81 335 2144 872
Fragments arranged by size:
2144 872 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 3779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 757 671
Size: 86 3693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 1132 1132
Size: 3779
BsaBI GATnn'nnATC
Cuts at: 6 1293 3330 6
Size: 1287 2037 455
Fragments arranged by size:
2037 1287 455
BsaHI Gr'CG_yC
Cuts at: 1210 1458 2160 3656 1210
Size: 248 702 1496 1333
Fragments arranged by size:
1496 1333 702 248
BsaWI w'CCGG_w
Cuts at: 968 1489 2167 2441 2588 2999 968
Size: 521 678 274 147 411 1748
Fragments arranged by size:
1748 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 730 733 736 544
Size: 186 3 3 3587
Fragments arranged by size:
3587 186 3 3
BseYI C'CCAG_C
Cuts at: 517 1204 1962 2539 3035 517
Size: 687 758 577 496 1261
Fragments arranged by size:
1261 758 687 577 496
BsiEI CG_ry'CG
Cuts at: 104 1321 1367 2575 104
Size: 1217 46 1208 1308
Fragments arranged by size:
1308 1217 1208 46
BsiHKAI G_wGCw'C
Cuts at: 371 430 793 1571 1761 2553 371
Size: 59 363 778 190 792 1597
Fragments arranged by size:
1597 792 778 363 190 59
Bsp1286I G_dGCh'C
Cuts at: 371 430 793 1404 1497 1571 1761 1823 2553
Size: 59 363 611 93 74 190 62 730
Cuts at: 2553 371
Size: 1597
Fragments arranged by size:
1597 730 611 363 190 93 74 62
59
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1043 1345 1726 829
Size: 214 302 381 2882
Fragments arranged by size:
2882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2071 2125 3609 3774 50
Size: 2021 54 1484 165 55
Fragments arranged by size:
2021 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1691 1691
Size: 3779
BsrFI r'CCGG_y
Cuts at: 1777 1958 2167 1777
Size: 181 209 3389
Fragments arranged by size:
3389 209 181
BssSI C'ACGA_G
Cuts at: 2050 2408 2050
Size: 358 3421
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1156
Size: 3779
BstBI TT'CG_AA
Cuts at: 2140 2140
Size: 3779
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 3779
BstYI r'GATC_y
Cuts at: 1 804 1271 1294 1629 1875 2887 2973 2985
Size: 803 467 23 335 246 1012 86 12
Cuts at: 2985 3331 1
Size: 346 449
Fragments arranged by size:
1012 803 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 709 1729 1910 2073 709
Size: 1020 181 163 2415
Fragments arranged by size:
2415 1020 181 163
BtsI GCAGTG_nn'
Cuts at: 1022 1022
Size: 3779
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 296 758 1364 1538 1929 1956 3301 296
Size: 462 606 174 391 27 1345 774
Fragments arranged by size:
1345 774 606 462 391 174 27
EagI C'GGCC_G
Cuts at: 1364 1364
Size: 3779
EarI CTCTTCn'nnn_
Cuts at: 418 722 1802 2012 418
Size: 304 1080 210 2185
Fragments arranged by size:
2185 1080 304 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1987 2297 2443 3589 1987
Size: 310 146 1146 2177
Fragments arranged by size:
2177 1146 310 146
EcoICRI GAG'CTC
Cuts at: 791 791
Size: 3779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 757 1604 2036 2783 3051 671
Size: 86 847 432 747 268 1399
Fragments arranged by size:
1399 847 747 432 268 86
EcoO109I rG'GnC_Cy
Cuts at: 632 918 632
Size: 286 3493
EcoRI G'AATT_C
Cuts at: 608 783 608
Size: 175 3604
EcoRV GAT'ATC
Cuts at: 216 216
Size: 3779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3779
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 1213 1461 2483 3462 1213
Size: 248 1022 979 1530
Fragments arranged by size:
1530 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1621 1651 1653 1683 1621
Size: 30 2 30 3717
Fragments arranged by size:
3717 30 30 2
HincII GTy'rAC
Cuts at: 198 818 198
Size: 620 3159
KasI G'GCGC_C
Cuts at: 1209 1457 1209
Size: 248 3531
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2634 2450
Size: 184 3595
MscI TGG'CCA
Cuts at: 298 760 1540 298
Size: 462 780 2537
Fragments arranged by size:
2537 780 462
MslI CAynn'nnrTG
Cuts at: 376 433 1768 1895 376
Size: 57 1335 127 2260
Fragments arranged by size:
2260 1335 127 57
MspA1I CmG'CkG
Cuts at: 890 1204 1564 2577 2822 890
Size: 314 360 1013 245 1847
Fragments arranged by size:
1847 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1960 1960
Size: 3779
NarI GG'CG_CC
Cuts at: 1210 1458 1210
Size: 248 3531
NcoI C'CATG_G
Cuts at: 107 107
Size: 3779
NgoMIV G'CCGG_C
Cuts at: 1958 1958
Size: 3779
NsiI A_TGCA'T
Cuts at: 345 345
Size: 3779
NspI r_CATG'y
Cuts at: 832 1332 2239 832
Size: 500 907 2372
Fragments arranged by size:
2372 907 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PfoI T'CCnGG_A
Cuts at: 687 687
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PstI C_TGCA'G
Cuts at: 422 826 1511 422
Size: 404 685 2690
Fragments arranged by size:
2690 685 404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 1204 1564 1204
Size: 360 3419
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 793 793
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 418 1802 2012 418
Size: 1384 210 2185
Fragments arranged by size:
2185 1384 210
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SfcI C'TryA_G
Cuts at: 33 418 822 1507 2500 2691 33
Size: 385 404 685 993 191 1121
Fragments arranged by size:
1121 993 685 404 385 191
SfoI GGC'GCC
Cuts at: 1211 1459 1211
Size: 248 3531
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 801 801
Size: 3779
SmlI C'TyrA_G
Cuts at: 934 1849 2341 2603 934
Size: 915 492 262 2110
Fragments arranged by size:
2110 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
StyI C'CwwG_G
Cuts at: 107 299 635 913 1267 107
Size: 192 336 278 354 2619
Fragments arranged by size:
2619 354 336 278 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 663 1362 1495 1954 2163 663
Size: 699 133 459 209 2279
Fragments arranged by size:
2279 699 459 209 133
TatI w'GTAC_w
Cuts at: 3294 3294
Size: 3779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 810 810
Size: 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1274 1274
Size: 3779
XmaI C'CCGG_G
Cuts at: 799 799
Size: 3779
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI