Restriction map for
pF1KB8108
(Circular) (Six-base) MAPSORT of: pF1KB8108.seq Check: 1909 from: 1 to: 4547
pF1KB8108 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2372 2804 3551 3819 1423
Size: 949 432 747 268 2151
Fragments arranged by size:
2151 949 747 432 268
AflIII A'CryG_T
Cuts at: 1278 1507 1899 3003 3980 1278
Size: 229 392 1104 977 1845
Fragments arranged by size:
1845 1104 977 392 229
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 974 1006 1475 1507 422
Size: 32 520 32 469 32 3462
Fragments arranged by size:
3462 520 469 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1852 3419 1852
Size: 1567 2980
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1263 1551 3900 4156 1263
Size: 288 2349 256 1654
Fragments arranged by size:
2349 1654 288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 1567 1567
Size: 4547
BamHI G'GATC_C
Cuts at: 162 1572 162
Size: 1410 3137
BanI G'GyrC_C
Cuts at: 1563 1977 2225 2260 1563
Size: 414 248 35 3850
Fragments arranged by size:
3850 414 248 35
BanII G_rGCy'C
Cuts at: 1561 2591 1561
Size: 1030 3517
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbsI GAAGACnn'nnnn_
Cuts at: 716 1066 716
Size: 350 4197
BbvCI CC'TCA_GC
Cuts at: 200 200
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 488 2445 3206 4547 488
Size: 1957 761 1341 488
Fragments arranged by size:
1957 1341 761 488
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 196 1290 196
Size: 1094 3453
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 2172 2265 3321 2172
Size: 93 1056 3398
Fragments arranged by size:
3398 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1759 1840 2175 4319 1759
Size: 81 335 2144 1987
Fragments arranged by size:
2144 1987 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
Bpu10I CC'TnA_GC
Cuts at: 200 200
Size: 4547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3094 3392 1723
Size: 879 492 298 2878
Fragments arranged by size:
2878 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4251 4251
Size: 4547
BsaAI yAC'GTr
Cuts at: 654 1900 654
Size: 1246 3301
BsaBI GATnn'nnATC
Cuts at: 6 691 1436 2061 4098 6
Size: 685 745 625 2037 455
Fragments arranged by size:
2037 745 685 625 455
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 281 311 435 465 281
Size: 30 124 30 4363
Fragments arranged by size:
4363 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 1544 136
Size: 1408 3139
BseYI C'CCAG_C
Cuts at: 1972 2730 3307 3803 1972
Size: 758 577 496 2716
Fragments arranged by size:
2716 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1561 2339 2529 3321 1561
Size: 778 190 792 2787
Fragments arranged by size:
2787 792 778 190
BsmI GAATG_Cn'
Cuts at: 514 514
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 1561 2172 2265 2339 2529 2591 3321 1561
Size: 611 93 74 190 62 730 2787
Fragments arranged by size:
2787 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 1230 3723 1230
Size: 2493 2054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1597 1811 2113 2494 1597
Size: 214 302 381 3650
Fragments arranged by size:
3650 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2839 2893 4377 4542 50
Size: 2789 54 1484 165 55
Fragments arranged by size:
2789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 814 2545 2726 2935 814
Size: 1731 181 209 2426
Fragments arranged by size:
2426 1731 209 181
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 151 151
Size: 4547
BstYI r'GATC_y
Cuts at: 1 162 1431 1572 2039 2062 2397 2643 3655
Size: 161 1269 141 467 23 335 246 1012
Cuts at: 3655 3741 3753 4099 1
Size: 86 12 346 449
Fragments arranged by size:
1269 1012 467 449 346 335 246 161
141 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 392 2497 2678 2841 392
Size: 2105 181 163 2098
Fragments arranged by size:
2105 2098 181 163
BtsI GCAGTG_nn'
Cuts at: 1790 1790
Size: 4547
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 2132 2306 2697 2724 4069 2132
Size: 174 391 27 1345 2610
Fragments arranged by size:
2610 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 232 418 590 2570 2780 232
Size: 186 172 1980 210 1999
Fragments arranged by size:
1999 1980 210 186 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3065 3211 4357 2755
Size: 310 146 1146 2945
Fragments arranged by size:
2945 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2372 2804 3551 3819 1423
Size: 949 432 747 268 2151
Fragments arranged by size:
2151 949 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 247 1686 247
Size: 1439 3108
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 1981 2229 3251 4230 1981
Size: 248 1022 979 2298
Fragments arranged by size:
2298 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 439 471 764 796 2389 2419 2421
Size: 32 139 32 293 32 1593 30 2
Cuts at: 2421 2451 268
Size: 30 2364
Fragments arranged by size:
2364 1593 293 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MfeI C'AATT_G
Cuts at: 1103 1193 1103
Size: 90 4457
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 686 1254 1528 3218 3402 299
Size: 387 568 274 1690 184 1444
Fragments arranged by size:
1690 1444 568 387 274 184
MscI TGG'CCA
Cuts at: 2308 2308
Size: 4547
MslI CAynn'nnrTG
Cuts at: 149 875 1446 2536 2663 149
Size: 726 571 1090 127 2033
Fragments arranged by size:
2033 1090 726 571 127
MspA1I CmG'CkG
Cuts at: 544 1658 1972 2332 3345 3590 544
Size: 1114 314 360 1013 245 1501
Fragments arranged by size:
1501 1114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2728 2728
Size: 4547
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 107 107
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 2726 2726
Size: 4547
NspI r_CATG'y
Cuts at: 1126 1282 1511 1600 2100 3007 1126
Size: 156 229 89 500 907 2666
Fragments arranged by size:
2666 907 500 229 156 89
PciI A'CATG_T
Cuts at: 1278 1507 3003 1278
Size: 229 1496 2822
Fragments arranged by size:
2822 1496 229
PfoI T'CCnGG_A
Cuts at: 136 136
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PstI C_TGCA'G
Cuts at: 258 627 1594 2279 258
Size: 369 967 685 2526
Fragments arranged by size:
2526 967 685 369
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 544 1972 2332 544
Size: 1428 360 2759
Fragments arranged by size:
2759 1428 360
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 232 2570 2780 232
Size: 2338 210 1999
Fragments arranged by size:
2338 1999 210
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 254 623 1590 2275 3268 3459 33
Size: 221 369 967 685 993 191 1121
Fragments arranged by size:
1121 993 967 685 369 221 191
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 1569 1569
Size: 4547
SmlI C'TyrA_G
Cuts at: 1702 2617 3109 3371 1702
Size: 915 492 262 2878
Fragments arranged by size:
2878 915 492 262
SnaBI TAC'GTA
Cuts at: 654 654
Size: 4547
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 755 755
Size: 4547
StyI C'CwwG_G
Cuts at: 107 1681 2035 107
Size: 1574 354 2619
Fragments arranged by size:
2619 1574 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 537 2130 2263 2722 2931 537
Size: 1593 133 459 209 2153
Fragments arranged by size:
2153 1593 459 209 133
TatI w'GTAC_w
Cuts at: 1210 4062 1210
Size: 2852 1695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 900 932 1121 1153 4064 4096 281
Size: 32 587 32 189 32 2911 32 732
Fragments arranged by size:
2911 732 587 189 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2042 114
Size: 1928 2619
XmaI C'CCGG_G
Cuts at: 1567 1567
Size: 4547
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI