Restriction map for
pF1KB8112
(Circular) (Six-base) MAPSORT of: pF1KB8112.seq Check: 2604 from: 1 to: 3569
pF1KB8112 3569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 347 607 347
Size: 260 3309
Acc65I G'GTAC_C
Cuts at: 585 585
Size: 3569
AclI AA'CG_TT
Cuts at: 3409 3409
Size: 3569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1826 2573 2841 1394
Size: 432 747 268 2122
Fragments arranged by size:
2122 747 432 268
AflIII A'CryG_T
Cuts at: 921 2025 3002 921
Size: 1104 977 1488
Fragments arranged by size:
1488 1104 977
AgeI A'CCGG_T
Cuts at: 1957 1957
Size: 3569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 3569
AlwNI CAG_nnn'CTG
Cuts at: 169 874 2441 169
Size: 705 1567 1297
Fragments arranged by size:
1567 1297 705
ApaLI G'TGCA_C
Cuts at: 2339 2339
Size: 3569
ApoI r'AATT_y
Cuts at: 329 573 2922 3178 329
Size: 244 2349 256 720
Fragments arranged by size:
2349 720 256 244
AseI AT'TA_AT
Cuts at: 20 20
Size: 3569
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
AvaI C'yCGr_G
Cuts at: 589 589
Size: 3569
BamHI G'GATC_C
Cuts at: 594 594
Size: 3569
BanI G'GyrC_C
Cuts at: 585 999 1247 1282 585
Size: 414 248 35 2872
Fragments arranged by size:
2872 414 248 35
BanII G_rGCy'C
Cuts at: 583 1613 583
Size: 1030 2539
BbeI G_GCGC'C
Cuts at: 1003 1251 1003
Size: 248 3321
BbsI GAAGACnn'nnnn_
Cuts at: 502 502
Size: 3569
BciVI GTATCCnnnnn_n'
Cuts at: 1467 2228 3569 1467
Size: 761 1341 1467
Fragments arranged by size:
1467 1341 761
BclI T'GATC_A
Cuts at: 1089 1089
Size: 3569
BglII A'GATC_T
Cuts at: 1084 3121 1084
Size: 2037 1532
BlpI GC'TnA_GC
Cuts at: 681 681
Size: 3569
Bme1580I G_kGCm'C
Cuts at: 1194 1287 2343 1194
Size: 93 1056 2420
Fragments arranged by size:
2420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 781 862 1197 3341 781
Size: 81 335 2144 1009
Fragments arranged by size:
2144 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1624 2116 2414 745
Size: 879 492 298 1900
Fragments arranged by size:
1900 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3273 3273
Size: 3569
BsaAI yAC'GTr
Cuts at: 922 922
Size: 3569
BsaBI GATnn'nnATC
Cuts at: 6 1083 3120 6
Size: 1077 2037 455
Fragments arranged by size:
2037 1077 455
BsaHI Gr'CG_yC
Cuts at: 1000 1248 1950 3446 1000
Size: 248 702 1496 1123
Fragments arranged by size:
1496 1123 702 248
BsaWI w'CCGG_w
Cuts at: 758 1279 1957 2231 2378 2789 758
Size: 521 678 274 147 411 1538
Fragments arranged by size:
1538 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 143 396 994 1752 2329 2825 143
Size: 253 598 758 577 496 887
Fragments arranged by size:
887 758 598 577 496 253
BsiEI CG_ry'CG
Cuts at: 104 1111 1157 2365 104
Size: 1007 46 1208 1308
Fragments arranged by size:
1308 1208 1007 46
BsiHKAI G_wGCw'C
Cuts at: 450 583 1361 1551 2343 450
Size: 133 778 190 792 1676
Fragments arranged by size:
1676 792 778 190 133
Bsp1286I G_dGCh'C
Cuts at: 450 583 1194 1287 1361 1551 1613 2343 450
Size: 133 611 93 74 190 62 730 1676
Fragments arranged by size:
1676 730 611 190 133 93 74 62
BspEI T'CCGG_A
Cuts at: 2789 2789
Size: 3569
BspHI T'CATG_A
Cuts at: 320 2745 320
Size: 2425 1144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 619 833 1135 1516 619
Size: 214 302 381 2672
Fragments arranged by size:
2672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1861 1915 3399 3564 50
Size: 1811 54 1484 165 55
Fragments arranged by size:
1811 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 221 1481 221
Size: 1260 2309
BsrFI r'CCGG_y
Cuts at: 1567 1748 1957 1567
Size: 181 209 3179
Fragments arranged by size:
3179 209 181
BssSI C'ACGA_G
Cuts at: 1840 2198 1840
Size: 358 3211
BstAPI GCAn_nnn'nTGC
Cuts at: 946 946
Size: 3569
BstBI TT'CG_AA
Cuts at: 1930 1930
Size: 3569
BstYI r'GATC_y
Cuts at: 1 407 594 1061 1084 1419 1665 2677 2763
Size: 406 187 467 23 335 246 1012 86
Cuts at: 2763 2775 3121 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 406 346 335 246 187
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 233 233
Size: 3569
BtgI C'CryG_G
Cuts at: 107 107
Size: 3569
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1519 1700 1863 1519
Size: 181 163 3225
Fragments arranged by size:
3225 181 163
BtsI GCAGTG_nn'
Cuts at: 400 496 812 400
Size: 96 316 3157
Fragments arranged by size:
3157 316 96
ClaI AT'CG_AT
Cuts at: 427 427
Size: 3569
DraI TTT'AAA
Cuts at: 264 568 264
Size: 304 3265
DrdI GACnn_nn'nnGTC
Cuts at: 1275 2133 1275
Size: 858 2711
EaeI y'GGCC_r
Cuts at: 110 1154 1328 1719 1746 3091 110
Size: 1044 174 391 27 1345 588
Fragments arranged by size:
1345 1044 588 391 174 27
EagI C'GGCC_G
Cuts at: 1154 1154
Size: 3569
EarI CTCTTCn'nnn_
Cuts at: 1592 1802 1592
Size: 210 3359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1777 2087 2233 3379 1777
Size: 310 146 1146 1967
Fragments arranged by size:
1967 1146 310 146
EcoICRI GAG'CTC
Cuts at: 581 581
Size: 3569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1826 2573 2841 1394
Size: 432 747 268 2122
Fragments arranged by size:
2122 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 708 708
Size: 3569
EcoRI G'AATT_C
Cuts at: 573 573
Size: 3569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1588 1588
Size: 3569
FspI TGC'GCA
Cuts at: 1350 1350
Size: 3569
HaeII r_GCGC'y
Cuts at: 1003 1251 2273 3252 1003
Size: 248 1022 979 1320
Fragments arranged by size:
1320 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 1411 1441 1443 1473 376
Size: 32 1003 30 2 30 2472
Fragments arranged by size:
2472 1003 32 30 30 2
HincII GTy'rAC
Cuts at: 608 608
Size: 3569
KasI G'GCGC_C
Cuts at: 999 1247 999
Size: 248 3321
KpnI G_GTAC'C
Cuts at: 589 589
Size: 3569
MluI A'CGCG_T
Cuts at: 3002 3002
Size: 3569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2424 2240
Size: 184 3385
MscI TGG'CCA
Cuts at: 1330 1330
Size: 3569
MslI CAynn'nnrTG
Cuts at: 341 1558 1685 341
Size: 1217 127 2225
Fragments arranged by size:
2225 1217 127
MspA1I CmG'CkG
Cuts at: 680 994 1354 2367 2612 680
Size: 314 360 1013 245 1637
Fragments arranged by size:
1637 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1750 1750
Size: 3569
NarI GG'CG_CC
Cuts at: 1000 1248 1000
Size: 248 3321
NcoI C'CATG_G
Cuts at: 107 107
Size: 3569
NgoMIV G'CCGG_C
Cuts at: 1748 1748
Size: 3569
NspI r_CATG'y
Cuts at: 622 1122 2029 622
Size: 500 907 2162
Fragments arranged by size:
2162 907 500
PciI A'CATG_T
Cuts at: 2025 2025
Size: 3569
PflMI CCAn_nnn'nTGG
Cuts at: 169 169
Size: 3569
PmeI GTTT'AAAC
Cuts at: 568 568
Size: 3569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 3537
PstI C_TGCA'G
Cuts at: 407 616 1301 407
Size: 209 685 2675
Fragments arranged by size:
2675 685 209
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3569
PvuII CAG'CTG
Cuts at: 994 1354 994
Size: 360 3209
RsrII CG'GwC_CG
Cuts at: 1764 1764
Size: 3569
SacI G_AGCT'C
Cuts at: 583 583
Size: 3569
SalI G'TCGA_C
Cuts at: 606 606
Size: 3569
SapI GCTCTTCn'nnn_
Cuts at: 1592 1802 1592
Size: 210 3359
SbfI CC_TGCA'GG
Cuts at: 616 616
Size: 3569
ScaI AGT'ACT
Cuts at: 3086 3086
Size: 3569
SfcI C'TryA_G
Cuts at: 33 403 612 1297 2290 2481 33
Size: 370 209 685 993 191 1121
Fragments arranged by size:
1121 993 685 370 209 191
SfoI GGC'GCC
Cuts at: 1001 1249 1001
Size: 248 3321
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
SmaI CCC'GGG
Cuts at: 591 591
Size: 3569
SmlI C'TyrA_G
Cuts at: 724 1639 2131 2393 724
Size: 915 492 262 1900
Fragments arranged by size:
1900 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3569
SphI G_CATG'C
Cuts at: 622 1122 622
Size: 500 3069
SspI AAT'ATT
Cuts at: 561 561
Size: 3569
StuI AGG'CCT
Cuts at: 436 436
Size: 3569
StyI C'CwwG_G
Cuts at: 107 703 1057 107
Size: 596 354 2619
Fragments arranged by size:
2619 596 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 1285 1744 1953 1152
Size: 133 459 209 2768
Fragments arranged by size:
2768 459 209 133
TatI w'GTAC_w
Cuts at: 3084 3084
Size: 3569
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 392 424 3086 3118 392
Size: 32 2662 32 843
Fragments arranged by size:
2662 843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1366 1366
Size: 3569
XbaI T'CTAG_A
Cuts at: 600 600
Size: 3569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1064 1064
Size: 3569
XmaI C'CCGG_G
Cuts at: 589 589
Size: 3569
XmnI GAAnn'nnTTC
Cuts at: 164 164
Size: 3569
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI